Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA
The detection of protein/small molecule interactions plays important roles in drug discovery and protein/metabolite interactions in biology. In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP...
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| Veröffentlicht in: | Science China. Chemistry 2016-06, Vol.59 (6), p.770-775 |
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| creator | Wang, Min Li, Fangzhen Jiang, Bingying Xu, Junqiang Li, Shuo Xiang, Yun Yuan, Ruo |
| description | The detection of protein/small molecule interactions plays important roles in drug discovery and protein/metabolite interactions in biology. In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP) nanoprobes, we have developed a sensitive and simple colorimetric sensor for the detection of folate receptor, a highly expressed protein in many kinds of malignant tumors. The target folate receptor binds the folate moieties of the folate-linked ssDNA through high affinity interactions and protects the protein-bound ssDNA from digestion by exonuclease I. The protected ssDNA thus adsorbs the ((+)AuNP) through electrostatic interactions, leading to a red-to-blue color change of the sensing solution for sensitive colorimetric detection of folate receptor at the sub-nanomolar level. Besides, this colorimetric sensor shows high selectivity toward folate receptor against other control proteins. The developed sensor avoids the modification/conjugation of the AuNP nanoprobes and the involvement of any expensive instruments for signal transduction in protein detection. Featured with these obvious advantages, the colorimetric sensor strategy demonstrated herein can be easily expanded for sensitive and convenient detection of various protein/small molecule interactions. |
| doi_str_mv | 10.1007/s11426-016-5589-9 |
| format | Article |
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In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP) nanoprobes, we have developed a sensitive and simple colorimetric sensor for the detection of folate receptor, a highly expressed protein in many kinds of malignant tumors. The target folate receptor binds the folate moieties of the folate-linked ssDNA through high affinity interactions and protects the protein-bound ssDNA from digestion by exonuclease I. The protected ssDNA thus adsorbs the ((+)AuNP) through electrostatic interactions, leading to a red-to-blue color change of the sensing solution for sensitive colorimetric detection of folate receptor at the sub-nanomolar level. Besides, this colorimetric sensor shows high selectivity toward folate receptor against other control proteins. The developed sensor avoids the modification/conjugation of the AuNP nanoprobes and the involvement of any expensive instruments for signal transduction in protein detection. Featured with these obvious advantages, the colorimetric sensor strategy demonstrated herein can be easily expanded for sensitive and convenient detection of various protein/small molecule interactions.</description><identifier>ISSN: 1674-7291</identifier><identifier>EISSN: 1869-1870</identifier><identifier>DOI: 10.1007/s11426-016-5589-9</identifier><language>eng</language><publisher>Beijing: Science China Press</publisher><subject>Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Colorimetry ; Conjugation ; Gold ; Metabolites ; Nanoparticles ; Proteins ; Receptors ; Sensors ; Signal transduction ; Vitamin B</subject><ispartof>Science China. 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Chemistry</title><addtitle>Sci. China Chem</addtitle><description>The detection of protein/small molecule interactions plays important roles in drug discovery and protein/metabolite interactions in biology. In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP) nanoprobes, we have developed a sensitive and simple colorimetric sensor for the detection of folate receptor, a highly expressed protein in many kinds of malignant tumors. The target folate receptor binds the folate moieties of the folate-linked ssDNA through high affinity interactions and protects the protein-bound ssDNA from digestion by exonuclease I. The protected ssDNA thus adsorbs the ((+)AuNP) through electrostatic interactions, leading to a red-to-blue color change of the sensing solution for sensitive colorimetric detection of folate receptor at the sub-nanomolar level. Besides, this colorimetric sensor shows high selectivity toward folate receptor against other control proteins. The developed sensor avoids the modification/conjugation of the AuNP nanoprobes and the involvement of any expensive instruments for signal transduction in protein detection. Featured with these obvious advantages, the colorimetric sensor strategy demonstrated herein can be easily expanded for sensitive and convenient detection of various protein/small molecule interactions.</description><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Colorimetry</subject><subject>Conjugation</subject><subject>Gold</subject><subject>Metabolites</subject><subject>Nanoparticles</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Sensors</subject><subject>Signal transduction</subject><subject>Vitamin B</subject><issn>1674-7291</issn><issn>1869-1870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1rHSEUhqW00HCTH5Cd0LWtx7k64zKkHwmEZtOsxfHjxtTRqXoD-T39o_UyhXRTN4o8zzlHX4QugX4ESsdPFWDPBKEgCOeTJPINOoNJSALTSN_2sxj3ZGQS3qOLWp9oX8NA2cjP0O-HtGQbfHAW62Txmmto4dnFF2wedTn060OOFied8qpLCya6in0uuLq0odjkmEtYXCvBYOuaMy3khLPvXNTN4eKMW1t3noPGzZUlJB3xWvI_aF10jHjJ0ZljdCSG9LP3rvXz96tz9M7rWN3F332HHr5--XF9Q-7uv91eX90RM4BoxIxScBAw84EOYpJces-c1MxTz2bhJ26poV7O-1nKwc8WJj7DzAwYZr3dDzv0YavbJ_t1dLWpp3wsfdSq-t9NfATo4g7BRpmSay3Oq7U_XpcXBVSd4lBbHKrHoU5xqJPDNqd2Nh1cea38f-kPzEaRsA</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Wang, Min</creator><creator>Li, Fangzhen</creator><creator>Jiang, Bingying</creator><creator>Xu, Junqiang</creator><creator>Li, Shuo</creator><creator>Xiang, Yun</creator><creator>Yuan, Ruo</creator><general>Science China Press</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M2P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20160601</creationdate><title>Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA</title><author>Wang, Min ; Li, Fangzhen ; Jiang, Bingying ; Xu, Junqiang ; Li, Shuo ; Xiang, Yun ; Yuan, Ruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-c7965161b530368959ff2e9a2f0f2b6f85d0c0f9b4b993fbd185b1b2c1c2dfd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Colorimetry</topic><topic>Conjugation</topic><topic>Gold</topic><topic>Metabolites</topic><topic>Nanoparticles</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Sensors</topic><topic>Signal transduction</topic><topic>Vitamin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Li, Fangzhen</creatorcontrib><creatorcontrib>Jiang, Bingying</creatorcontrib><creatorcontrib>Xu, Junqiang</creatorcontrib><creatorcontrib>Li, Shuo</creatorcontrib><creatorcontrib>Xiang, Yun</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Science China. Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Min</au><au>Li, Fangzhen</au><au>Jiang, Bingying</au><au>Xu, Junqiang</au><au>Li, Shuo</au><au>Xiang, Yun</au><au>Yuan, Ruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA</atitle><jtitle>Science China. Chemistry</jtitle><stitle>Sci. China Chem</stitle><date>2016-06-01</date><risdate>2016</risdate><volume>59</volume><issue>6</issue><spage>770</spage><epage>775</epage><pages>770-775</pages><issn>1674-7291</issn><eissn>1869-1870</eissn><abstract>The detection of protein/small molecule interactions plays important roles in drug discovery and protein/metabolite interactions in biology. In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP) nanoprobes, we have developed a sensitive and simple colorimetric sensor for the detection of folate receptor, a highly expressed protein in many kinds of malignant tumors. The target folate receptor binds the folate moieties of the folate-linked ssDNA through high affinity interactions and protects the protein-bound ssDNA from digestion by exonuclease I. The protected ssDNA thus adsorbs the ((+)AuNP) through electrostatic interactions, leading to a red-to-blue color change of the sensing solution for sensitive colorimetric detection of folate receptor at the sub-nanomolar level. Besides, this colorimetric sensor shows high selectivity toward folate receptor against other control proteins. The developed sensor avoids the modification/conjugation of the AuNP nanoprobes and the involvement of any expensive instruments for signal transduction in protein detection. Featured with these obvious advantages, the colorimetric sensor strategy demonstrated herein can be easily expanded for sensitive and convenient detection of various protein/small molecule interactions.</abstract><cop>Beijing</cop><pub>Science China Press</pub><doi>10.1007/s11426-016-5589-9</doi><tpages>6</tpages></addata></record> |
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| subjects | Chemistry Chemistry and Materials Science Chemistry/Food Science Colorimetry Conjugation Gold Metabolites Nanoparticles Proteins Receptors Sensors Signal transduction Vitamin B |
| title | Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA |
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