Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide
Background Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydro...
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| Veröffentlicht in: | Sport sciences for health 2019-12, Vol.15 (3), p.569-576 |
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| creator | Ramezani, Somayeh Peeri, Maghsoud Azarbayjani, Mohammad Ali Dehghan, Firouzeh |
| description | Background
Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydrogen peroxide (H
2
O
2
).
Methods
48 male rats were assigned into six groups: H
2
O
2
, (H); H
2
O
2
+ D
3
, (HD); H
2
O
2
+ training, (HE); H
2
O
2
+ D
3
+ E, (HDE); dimethyl sulfoxide, (DMSO) and control intact. 1 mmol/kg of H
2
O
2
was injected three times per week. The exercising rats performed the program on a rodent’s treadmill for 8 weeks, 5 days a week. The HD and HDE groups received a daily dose of 0.5 µg for 8 weeks. The lung tissue was exposed and stored at − 80. Then, the RT-PCR method was employed to examine the gene expressions of BAX, BCL2, Caspase-3 and Bcl-2/Bax ratios.
Results
Results indicated that training, as well as a combination of training and vitamin D had a significant effect on BAX, BCL
2
and Bel
2
/Bax ratio in case of H
2
O
2
toxicity. The training and vitamin D groups both had no significant effect on Caspase-3 gene expression.
Conclusion
Based on the results of this research, it can be concluded that regular aerobic training alongside consumption of D
3
might result in significant alteration of the genes involved in apoptosis caused by H
2
O
2
presence in lung tissues. |
| doi_str_mv | 10.1007/s11332-019-00546-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2918497715</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2918497715</sourcerecordid><originalsourceid>FETCH-LOGICAL-c270t-e57871301d24586e000c91b5f676e826a501f9d7be84201ed86d0c42a22869903</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhiMEEqXwB5gsMRvOThzHbFX5lCqxwGy58SWktHaw04ou_HbcFomNySfreZ_TvVl2yeCaAcibyFiecwpMUQBRlBSOshGruKCM58Xxfi6oLBScZmcxLhJUCqFG2ffErjrXxSGYofOO-IZsusGkP3JHjLPEYPDzriYJSJxrb0nA2m8wbHfscu1aYnrfDz52kaxM-MAQSUqvzBJJkkaCX72PaMngyfvWBt-iI32yfnUWz7OTxiwjXvy-4-zt4f51-kRnL4_P08mM1lzCQFHISrIcmOWFqEoEgFqxuWhKWWLFSyOANcrKOVYFB4a2Ki3UBTecV6VSkI-zq4O3D_5zjXHQC78OLq3UXLGqUFIykSh-oOrgYwzY6D506aatZqB3PetDzzr1rPc96506P4Rigl2L4U_9T-oHYBOBFA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918497715</pqid></control><display><type>article</type><title>Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide</title><source>SpringerLink Journals</source><source>ProQuest Central</source><creator>Ramezani, Somayeh ; Peeri, Maghsoud ; Azarbayjani, Mohammad Ali ; Dehghan, Firouzeh</creator><creatorcontrib>Ramezani, Somayeh ; Peeri, Maghsoud ; Azarbayjani, Mohammad Ali ; Dehghan, Firouzeh</creatorcontrib><description>Background
Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydrogen peroxide (H
2
O
2
).
Methods
48 male rats were assigned into six groups: H
2
O
2
, (H); H
2
O
2
+ D
3
, (HD); H
2
O
2
+ training, (HE); H
2
O
2
+ D
3
+ E, (HDE); dimethyl sulfoxide, (DMSO) and control intact. 1 mmol/kg of H
2
O
2
was injected three times per week. The exercising rats performed the program on a rodent’s treadmill for 8 weeks, 5 days a week. The HD and HDE groups received a daily dose of 0.5 µg for 8 weeks. The lung tissue was exposed and stored at − 80. Then, the RT-PCR method was employed to examine the gene expressions of BAX, BCL2, Caspase-3 and Bcl-2/Bax ratios.
Results
Results indicated that training, as well as a combination of training and vitamin D had a significant effect on BAX, BCL
2
and Bel
2
/Bax ratio in case of H
2
O
2
toxicity. The training and vitamin D groups both had no significant effect on Caspase-3 gene expression.
Conclusion
Based on the results of this research, it can be concluded that regular aerobic training alongside consumption of D
3
might result in significant alteration of the genes involved in apoptosis caused by H
2
O
2
presence in lung tissues.</description><identifier>ISSN: 1824-7490</identifier><identifier>EISSN: 1825-1234</identifier><identifier>DOI: 10.1007/s11332-019-00546-0</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Aerobics ; Apoptosis ; Exercise ; Fitness equipment ; Food ; Human Physiology ; Laboratory animals ; Lungs ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Original Article ; Oxidative stress ; Physical fitness ; Pollutants ; Proteins ; Solvents ; Sports Medicine ; Toxicity ; Vitamin D</subject><ispartof>Sport sciences for health, 2019-12, Vol.15 (3), p.569-576</ispartof><rights>Springer-Verlag Italia S.r.l., part of Springer Nature 2019</rights><rights>Springer-Verlag Italia S.r.l., part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-e57871301d24586e000c91b5f676e826a501f9d7be84201ed86d0c42a22869903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11332-019-00546-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918497715?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,41464,42533,43781,51294</link.rule.ids></links><search><creatorcontrib>Ramezani, Somayeh</creatorcontrib><creatorcontrib>Peeri, Maghsoud</creatorcontrib><creatorcontrib>Azarbayjani, Mohammad Ali</creatorcontrib><creatorcontrib>Dehghan, Firouzeh</creatorcontrib><title>Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide</title><title>Sport sciences for health</title><addtitle>Sport Sci Health</addtitle><description>Background
Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydrogen peroxide (H
2
O
2
).
Methods
48 male rats were assigned into six groups: H
2
O
2
, (H); H
2
O
2
+ D
3
, (HD); H
2
O
2
+ training, (HE); H
2
O
2
+ D
3
+ E, (HDE); dimethyl sulfoxide, (DMSO) and control intact. 1 mmol/kg of H
2
O
2
was injected three times per week. The exercising rats performed the program on a rodent’s treadmill for 8 weeks, 5 days a week. The HD and HDE groups received a daily dose of 0.5 µg for 8 weeks. The lung tissue was exposed and stored at − 80. Then, the RT-PCR method was employed to examine the gene expressions of BAX, BCL2, Caspase-3 and Bcl-2/Bax ratios.
Results
Results indicated that training, as well as a combination of training and vitamin D had a significant effect on BAX, BCL
2
and Bel
2
/Bax ratio in case of H
2
O
2
toxicity. The training and vitamin D groups both had no significant effect on Caspase-3 gene expression.
Conclusion
Based on the results of this research, it can be concluded that regular aerobic training alongside consumption of D
3
might result in significant alteration of the genes involved in apoptosis caused by H
2
O
2
presence in lung tissues.</description><subject>Aerobics</subject><subject>Apoptosis</subject><subject>Exercise</subject><subject>Fitness equipment</subject><subject>Food</subject><subject>Human Physiology</subject><subject>Laboratory animals</subject><subject>Lungs</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Original Article</subject><subject>Oxidative stress</subject><subject>Physical fitness</subject><subject>Pollutants</subject><subject>Proteins</subject><subject>Solvents</subject><subject>Sports Medicine</subject><subject>Toxicity</subject><subject>Vitamin D</subject><issn>1824-7490</issn><issn>1825-1234</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kD1PwzAQhiMEEqXwB5gsMRvOThzHbFX5lCqxwGy58SWktHaw04ou_HbcFomNySfreZ_TvVl2yeCaAcibyFiecwpMUQBRlBSOshGruKCM58Xxfi6oLBScZmcxLhJUCqFG2ffErjrXxSGYofOO-IZsusGkP3JHjLPEYPDzriYJSJxrb0nA2m8wbHfscu1aYnrfDz52kaxM-MAQSUqvzBJJkkaCX72PaMngyfvWBt-iI32yfnUWz7OTxiwjXvy-4-zt4f51-kRnL4_P08mM1lzCQFHISrIcmOWFqEoEgFqxuWhKWWLFSyOANcrKOVYFB4a2Ki3UBTecV6VSkI-zq4O3D_5zjXHQC78OLq3UXLGqUFIykSh-oOrgYwzY6D506aatZqB3PetDzzr1rPc96506P4Rigl2L4U_9T-oHYBOBFA</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Ramezani, Somayeh</creator><creator>Peeri, Maghsoud</creator><creator>Azarbayjani, Mohammad Ali</creator><creator>Dehghan, Firouzeh</creator><general>Springer Milan</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20191201</creationdate><title>Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide</title><author>Ramezani, Somayeh ; Peeri, Maghsoud ; Azarbayjani, Mohammad Ali ; Dehghan, Firouzeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-e57871301d24586e000c91b5f676e826a501f9d7be84201ed86d0c42a22869903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aerobics</topic><topic>Apoptosis</topic><topic>Exercise</topic><topic>Fitness equipment</topic><topic>Food</topic><topic>Human Physiology</topic><topic>Laboratory animals</topic><topic>Lungs</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Original Article</topic><topic>Oxidative stress</topic><topic>Physical fitness</topic><topic>Pollutants</topic><topic>Proteins</topic><topic>Solvents</topic><topic>Sports Medicine</topic><topic>Toxicity</topic><topic>Vitamin D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramezani, Somayeh</creatorcontrib><creatorcontrib>Peeri, Maghsoud</creatorcontrib><creatorcontrib>Azarbayjani, Mohammad Ali</creatorcontrib><creatorcontrib>Dehghan, Firouzeh</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Sport sciences for health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramezani, Somayeh</au><au>Peeri, Maghsoud</au><au>Azarbayjani, Mohammad Ali</au><au>Dehghan, Firouzeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide</atitle><jtitle>Sport sciences for health</jtitle><stitle>Sport Sci Health</stitle><date>2019-12-01</date><risdate>2019</risdate><volume>15</volume><issue>3</issue><spage>569</spage><epage>576</epage><pages>569-576</pages><issn>1824-7490</issn><eissn>1825-1234</eissn><abstract>Background
Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydrogen peroxide (H
2
O
2
).
Methods
48 male rats were assigned into six groups: H
2
O
2
, (H); H
2
O
2
+ D
3
, (HD); H
2
O
2
+ training, (HE); H
2
O
2
+ D
3
+ E, (HDE); dimethyl sulfoxide, (DMSO) and control intact. 1 mmol/kg of H
2
O
2
was injected three times per week. The exercising rats performed the program on a rodent’s treadmill for 8 weeks, 5 days a week. The HD and HDE groups received a daily dose of 0.5 µg for 8 weeks. The lung tissue was exposed and stored at − 80. Then, the RT-PCR method was employed to examine the gene expressions of BAX, BCL2, Caspase-3 and Bcl-2/Bax ratios.
Results
Results indicated that training, as well as a combination of training and vitamin D had a significant effect on BAX, BCL
2
and Bel
2
/Bax ratio in case of H
2
O
2
toxicity. The training and vitamin D groups both had no significant effect on Caspase-3 gene expression.
Conclusion
Based on the results of this research, it can be concluded that regular aerobic training alongside consumption of D
3
might result in significant alteration of the genes involved in apoptosis caused by H
2
O
2
presence in lung tissues.</abstract><cop>Milan</cop><pub>Springer Milan</pub><doi>10.1007/s11332-019-00546-0</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | SpringerLink Journals; ProQuest Central |
| subjects | Aerobics Apoptosis Exercise Fitness equipment Food Human Physiology Laboratory animals Lungs Medicine Medicine & Public Health Metabolic Diseases Original Article Oxidative stress Physical fitness Pollutants Proteins Solvents Sports Medicine Toxicity Vitamin D |
| title | Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide |
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