Analysis of 19-norandrosterone in human urine by gas chromatography-isotope-dilution mass spectrometry : method adopted by LGC for participation in the Comité Consultatif pour la Quantité de Matière (CCQM) Pilot Study P68
The method used at LGC for analysis of “total” 19-norandrosterone (19-norandrosterone glucuronide plus “free” 19-norandrosterone) in urine for the Comité Consultatif pour la Quantité de Matière Pilot Study (CCQM-P68) is described. The analytical method used was a modified version of the method devel...
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| Veröffentlicht in: | Accreditation and quality assurance 2007-09, Vol.12 (9), p.469-474 |
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| creator | MUSSELL, Chris WOLFF BRICHE, Celine S. J HOPLEY, Chris O'CONNOR, Gavin |
| description | The method used at LGC for analysis of “total” 19-norandrosterone (19-norandrosterone glucuronide plus “free” 19-norandrosterone) in urine for the Comité Consultatif pour la Quantité de Matière Pilot Study (CCQM-P68) is described. The analytical method used was a modified version of the method developed at the National Measurement Institute of Australia, which used a hydrolysis and derivatisation procedure first described by the German Sports University. This method is routinely used by World Anti-Doping Agency-accredited laboratories for sports drug testing. The main modifications made to the method were the use of 19-norandrosterone glucuronide as a calibration standard and 19-norandrosterone glucuronide-d4 as an isotopically labelled internal standard, and the use of a bench-top quadrupole gas chromatograph–mass spectrometer. The results produced by LGC (2.14 ± 0.15 ng g−1 expanded uncertainty, coverage factor k = 2) were in excellent agreement with those from other participating national metrology institutes and thus further validates the exact-matching isotope-dilution mass spectrometric procedures used at LGC for a wide range of reference measurement applications, including measurement of ng g−1 levels of steroids in a biological matrix. |
| doi_str_mv | 10.1007/s00769-007-0282-5 |
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The main modifications made to the method were the use of 19-norandrosterone glucuronide as a calibration standard and 19-norandrosterone glucuronide-d4 as an isotopically labelled internal standard, and the use of a bench-top quadrupole gas chromatograph–mass spectrometer. The results produced by LGC (2.14 ± 0.15 ng g−1 expanded uncertainty, coverage factor k = 2) were in excellent agreement with those from other participating national metrology institutes and thus further validates the exact-matching isotope-dilution mass spectrometric procedures used at LGC for a wide range of reference measurement applications, including measurement of ng g−1 levels of steroids in a biological matrix.</description><identifier>ISSN: 0949-1775</identifier><identifier>EISSN: 1432-0517</identifier><identifier>DOI: 10.1007/s00769-007-0282-5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Analytical chemistry ; Calibration ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography ; Dilution ; Exact sciences and technology ; Gas chromatographic methods ; Gas chromatography ; Laboratories ; Mass spectrometry ; Participation ; Potassium ; Quadrupoles ; Quality control ; Quality standards ; Reagents ; Scientific imaging ; Signal to noise ratio ; Spectrometric and optical methods ; Urine</subject><ispartof>Accreditation and quality assurance, 2007-09, Vol.12 (9), p.469-474</ispartof><rights>2007 INIST-CNRS</rights><rights>LGC Limited 2007 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-10ee0e33fcd3fb940690b179848a4b9761760c9c69fbdc4f22a920b5a7e2751c3</citedby><cites>FETCH-LOGICAL-c303t-10ee0e33fcd3fb940690b179848a4b9761760c9c69fbdc4f22a920b5a7e2751c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2917922749?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21388,21389,27924,27925,33530,33744,43659,43805,64385,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19087566$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>MUSSELL, Chris</creatorcontrib><creatorcontrib>WOLFF BRICHE, Celine S. 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J</au><au>HOPLEY, Chris</au><au>O'CONNOR, Gavin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of 19-norandrosterone in human urine by gas chromatography-isotope-dilution mass spectrometry : method adopted by LGC for participation in the Comité Consultatif pour la Quantité de Matière (CCQM) Pilot Study P68</atitle><jtitle>Accreditation and quality assurance</jtitle><date>2007-09-01</date><risdate>2007</risdate><volume>12</volume><issue>9</issue><spage>469</spage><epage>474</epage><pages>469-474</pages><issn>0949-1775</issn><eissn>1432-0517</eissn><abstract>The method used at LGC for analysis of “total” 19-norandrosterone (19-norandrosterone glucuronide plus “free” 19-norandrosterone) in urine for the Comité Consultatif pour la Quantité de Matière Pilot Study (CCQM-P68) is described. The analytical method used was a modified version of the method developed at the National Measurement Institute of Australia, which used a hydrolysis and derivatisation procedure first described by the German Sports University. This method is routinely used by World Anti-Doping Agency-accredited laboratories for sports drug testing. The main modifications made to the method were the use of 19-norandrosterone glucuronide as a calibration standard and 19-norandrosterone glucuronide-d4 as an isotopically labelled internal standard, and the use of a bench-top quadrupole gas chromatograph–mass spectrometer. The results produced by LGC (2.14 ± 0.15 ng g−1 expanded uncertainty, coverage factor k = 2) were in excellent agreement with those from other participating national metrology institutes and thus further validates the exact-matching isotope-dilution mass spectrometric procedures used at LGC for a wide range of reference measurement applications, including measurement of ng g−1 levels of steroids in a biological matrix.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00769-007-0282-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Analytical chemistry Calibration Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Dilution Exact sciences and technology Gas chromatographic methods Gas chromatography Laboratories Mass spectrometry Participation Potassium Quadrupoles Quality control Quality standards Reagents Scientific imaging Signal to noise ratio Spectrometric and optical methods Urine |
| title | Analysis of 19-norandrosterone in human urine by gas chromatography-isotope-dilution mass spectrometry : method adopted by LGC for participation in the Comité Consultatif pour la Quantité de Matière (CCQM) Pilot Study P68 |
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