Extraction, statistical optimization, and immunomodulatory activity of exopolysaccharide from seaweed-associated Bacillus megaterium DSKPDF CMST3
Seaweed-associated Bacillus megaterium DSKPDF CMST3 was screened for EPS production and its production was statistically optimized using central composite design and response surface methodology. Structural characterization of EPS was investigated by FT-IR and NMR spectroscopy. Furthermore, morpholo...
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| Veröffentlicht in: | Biomass conversion and biorefinery 2024-01, Vol.14 (1), p.405-418 |
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| creator | Sathishkumar, Ramamoorthy Thirumalaikumar, Eswaramoorthy Rajeswari, Mayavan Veeramuthu Arun, Jeganathan Vimal, Sugumar Babu, Mariavincent Michael Citarasu, Thavasimuthu |
| description | Seaweed-associated
Bacillus megaterium
DSKPDF CMST3 was screened for EPS production and its production was statistically optimized using central composite design and response surface methodology. Structural characterization of EPS was investigated by FT-IR and NMR spectroscopy. Furthermore, morphological structure of the EPS investigated by SEM demonstrated it to be less porous with flake-like structure. The XRD and TGA observations revealed the semi-crystalline nature of EPS. EPS-supplemented diet significantly enhanced immunological and hematological parameters in
Labeo rohita
. The immune gene expression (IL10, IL-1β, and TGFβ) were significantly upregulated in lymphoid organs of the fish. Moreover, the dietary supplementation of EPS showed significantly (
p
|
| doi_str_mv | 10.1007/s13399-021-02180-8 |
| format | Article |
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Bacillus megaterium
DSKPDF CMST3 was screened for EPS production and its production was statistically optimized using central composite design and response surface methodology. Structural characterization of EPS was investigated by FT-IR and NMR spectroscopy. Furthermore, morphological structure of the EPS investigated by SEM demonstrated it to be less porous with flake-like structure. The XRD and TGA observations revealed the semi-crystalline nature of EPS. EPS-supplemented diet significantly enhanced immunological and hematological parameters in
Labeo rohita
. The immune gene expression (IL10, IL-1β, and TGFβ) were significantly upregulated in lymphoid organs of the fish. Moreover, the dietary supplementation of EPS showed significantly (
p
< 0.001) higher survival rates of DII (71%) compared with basal diet (56.40%) fed fish against
Aeromonas hydrophila
infection. The result suggests that the exopolysaccharide can be applied as a fish feed supplement to enhance fish immunity and disease resistance against
A. hydrophila
.</description><identifier>ISSN: 2190-6815</identifier><identifier>EISSN: 2190-6823</identifier><identifier>DOI: 10.1007/s13399-021-02180-8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biotechnology ; Design optimization ; Diet ; Energy ; Fish ; Gene expression ; Immunology ; Infrared spectroscopy ; NMR spectroscopy ; Original Article ; Renewable and Green Energy ; Response surface methodology ; Seaweeds ; Structural analysis</subject><ispartof>Biomass conversion and biorefinery, 2024-01, Vol.14 (1), p.405-418</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-aa423a0c8da1aa5312c142d4e97f15388e2bea0784035a2af068884a5604837a3</citedby><cites>FETCH-LOGICAL-c319t-aa423a0c8da1aa5312c142d4e97f15388e2bea0784035a2af068884a5604837a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13399-021-02180-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13399-021-02180-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Sathishkumar, Ramamoorthy</creatorcontrib><creatorcontrib>Thirumalaikumar, Eswaramoorthy</creatorcontrib><creatorcontrib>Rajeswari, Mayavan Veeramuthu</creatorcontrib><creatorcontrib>Arun, Jeganathan</creatorcontrib><creatorcontrib>Vimal, Sugumar</creatorcontrib><creatorcontrib>Babu, Mariavincent Michael</creatorcontrib><creatorcontrib>Citarasu, Thavasimuthu</creatorcontrib><title>Extraction, statistical optimization, and immunomodulatory activity of exopolysaccharide from seaweed-associated Bacillus megaterium DSKPDF CMST3</title><title>Biomass conversion and biorefinery</title><addtitle>Biomass Conv. Bioref</addtitle><description>Seaweed-associated
Bacillus megaterium
DSKPDF CMST3 was screened for EPS production and its production was statistically optimized using central composite design and response surface methodology. Structural characterization of EPS was investigated by FT-IR and NMR spectroscopy. Furthermore, morphological structure of the EPS investigated by SEM demonstrated it to be less porous with flake-like structure. The XRD and TGA observations revealed the semi-crystalline nature of EPS. EPS-supplemented diet significantly enhanced immunological and hematological parameters in
Labeo rohita
. The immune gene expression (IL10, IL-1β, and TGFβ) were significantly upregulated in lymphoid organs of the fish. Moreover, the dietary supplementation of EPS showed significantly (
p
< 0.001) higher survival rates of DII (71%) compared with basal diet (56.40%) fed fish against
Aeromonas hydrophila
infection. The result suggests that the exopolysaccharide can be applied as a fish feed supplement to enhance fish immunity and disease resistance against
A. hydrophila
.</description><subject>Biotechnology</subject><subject>Design optimization</subject><subject>Diet</subject><subject>Energy</subject><subject>Fish</subject><subject>Gene expression</subject><subject>Immunology</subject><subject>Infrared spectroscopy</subject><subject>NMR spectroscopy</subject><subject>Original Article</subject><subject>Renewable and Green Energy</subject><subject>Response surface methodology</subject><subject>Seaweeds</subject><subject>Structural analysis</subject><issn>2190-6815</issn><issn>2190-6823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kM1KxTAQhYsoeFFfwFXArdVJ0p90qVevioqCug5jmmoubVOTVK1v4Rvba0V3LoYZZs45A18U7VI4oAD5oaecF0UMjK5KQCzWohmjBcSZYHz9d6bpZrTj_RIAGM-54DCLPk_fg0MVjG33iQ8YjA9GYU1sF0xjPnC6YFsS0zR9axtb9jUG6waysr2aMBBbEf1uO1sPHpV6RmdKTSpnG-I1vmldxui9VQaDLskxKlPXvSeNfhoXzvQNObm7vD1ZkPn13T3fjjYqrL3e-elb0cPi9H5-Hl_dnF3Mj65ixWkRYsSEcQQlSqSIKadM0YSViS7yiqZcCM0eNUIuEuApMqwgE0IkmGaQCJ4j34r2ptzO2Zde-yCXtnft-FKygmYJh7QoRhWbVMpZ752uZOdMg26QFOSKvpzoy5G8_KYvxWjik8mP4vZJu7_of1xfpXiJ5A</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Sathishkumar, Ramamoorthy</creator><creator>Thirumalaikumar, Eswaramoorthy</creator><creator>Rajeswari, Mayavan Veeramuthu</creator><creator>Arun, Jeganathan</creator><creator>Vimal, Sugumar</creator><creator>Babu, Mariavincent Michael</creator><creator>Citarasu, Thavasimuthu</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20240101</creationdate><title>Extraction, statistical optimization, and immunomodulatory activity of exopolysaccharide from seaweed-associated Bacillus megaterium DSKPDF CMST3</title><author>Sathishkumar, Ramamoorthy ; Thirumalaikumar, Eswaramoorthy ; Rajeswari, Mayavan Veeramuthu ; Arun, Jeganathan ; Vimal, Sugumar ; Babu, Mariavincent Michael ; Citarasu, Thavasimuthu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-aa423a0c8da1aa5312c142d4e97f15388e2bea0784035a2af068884a5604837a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biotechnology</topic><topic>Design optimization</topic><topic>Diet</topic><topic>Energy</topic><topic>Fish</topic><topic>Gene expression</topic><topic>Immunology</topic><topic>Infrared spectroscopy</topic><topic>NMR spectroscopy</topic><topic>Original Article</topic><topic>Renewable and Green Energy</topic><topic>Response surface methodology</topic><topic>Seaweeds</topic><topic>Structural analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Sathishkumar, Ramamoorthy</creatorcontrib><creatorcontrib>Thirumalaikumar, Eswaramoorthy</creatorcontrib><creatorcontrib>Rajeswari, Mayavan Veeramuthu</creatorcontrib><creatorcontrib>Arun, Jeganathan</creatorcontrib><creatorcontrib>Vimal, Sugumar</creatorcontrib><creatorcontrib>Babu, Mariavincent Michael</creatorcontrib><creatorcontrib>Citarasu, Thavasimuthu</creatorcontrib><collection>CrossRef</collection><jtitle>Biomass conversion and biorefinery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sathishkumar, Ramamoorthy</au><au>Thirumalaikumar, Eswaramoorthy</au><au>Rajeswari, Mayavan Veeramuthu</au><au>Arun, Jeganathan</au><au>Vimal, Sugumar</au><au>Babu, Mariavincent Michael</au><au>Citarasu, Thavasimuthu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction, statistical optimization, and immunomodulatory activity of exopolysaccharide from seaweed-associated Bacillus megaterium DSKPDF CMST3</atitle><jtitle>Biomass conversion and biorefinery</jtitle><stitle>Biomass Conv. Bioref</stitle><date>2024-01-01</date><risdate>2024</risdate><volume>14</volume><issue>1</issue><spage>405</spage><epage>418</epage><pages>405-418</pages><issn>2190-6815</issn><eissn>2190-6823</eissn><abstract>Seaweed-associated
Bacillus megaterium
DSKPDF CMST3 was screened for EPS production and its production was statistically optimized using central composite design and response surface methodology. Structural characterization of EPS was investigated by FT-IR and NMR spectroscopy. Furthermore, morphological structure of the EPS investigated by SEM demonstrated it to be less porous with flake-like structure. The XRD and TGA observations revealed the semi-crystalline nature of EPS. EPS-supplemented diet significantly enhanced immunological and hematological parameters in
Labeo rohita
. The immune gene expression (IL10, IL-1β, and TGFβ) were significantly upregulated in lymphoid organs of the fish. Moreover, the dietary supplementation of EPS showed significantly (
p
< 0.001) higher survival rates of DII (71%) compared with basal diet (56.40%) fed fish against
Aeromonas hydrophila
infection. The result suggests that the exopolysaccharide can be applied as a fish feed supplement to enhance fish immunity and disease resistance against
A. hydrophila
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s13399-021-02180-8</doi><tpages>14</tpages></addata></record> |
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| subjects | Biotechnology Design optimization Diet Energy Fish Gene expression Immunology Infrared spectroscopy NMR spectroscopy Original Article Renewable and Green Energy Response surface methodology Seaweeds Structural analysis |
| title | Extraction, statistical optimization, and immunomodulatory activity of exopolysaccharide from seaweed-associated Bacillus megaterium DSKPDF CMST3 |
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