Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment

Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovativ...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Sustainability 2023-12, Vol.15 (24), p.16616
Hauptverfasser: Manríquez-Zúñiga, Aranza Nallely, de la Torre, Argelia Rosillo, Valdés-Santiago, Laura, Hernández-Bustos, Diana A, Cuéllar-Sojo, Samantha, Hernández-Rayas, Angélica, Perez-Vega, Samuel, Molina-Guerrero, Carlos Eduardo
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 24
container_start_page 16616
container_title Sustainability
container_volume 15
creator Manríquez-Zúñiga, Aranza Nallely
de la Torre, Argelia Rosillo
Valdés-Santiago, Laura
Hernández-Bustos, Diana A
Cuéllar-Sojo, Samantha
Hernández-Rayas, Angélica
Perez-Vega, Samuel
Molina-Guerrero, Carlos Eduardo
description Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovative products. In this research, we used and characterized dynamic maceration to extract BACs and CBBs from broccoli leaves (BLs). A central composite design (CCD) was selected to evaluate the effect of temperature (from 4 °C to 70 °C), ethanol concentration (from 30% to 70% (v/v)), and exposition time (15 to 60 min) on total phenolic content (TPC) (mg of gallic acid equivalents (GAEs) per 100 g of dry biomass (db)). A confirmation experiment (CE) was performed to reproduce the optimal conditions (50 °C, 36.92 min, and 30% (v/v)) for BAC extraction. Results indicated a GAE concentration of 112.95 ± 0.92 mg/100 g db, while the statistical model predicted a value of 111.87 mg of GAEs/100 g db (error of 0.95%) with a rate constant (k) value of 0.0154 mg/g·min (R2 of 0.9894). BACs and CBBs were identified with gas chromatography–electron impact mass spectrometry detecting l-isoleucine, l-leucine, malonic acid, and succinic acid, among others. Finally, a life cycle inventory (LCI) was developed to determine global warming (GW) and water consumption (WC), among others, for 10 g of BL extract. Findings reported herein prove the sustainability of eco-friendly extraction of BACs and CBBs for the effective use of agricultural by-products.
doi_str_mv 10.3390/su152416616
format Article
fullrecord <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2904935817</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A779344050</galeid><sourcerecordid>A779344050</sourcerecordid><originalsourceid>FETCH-LOGICAL-c287t-46c118bcc9de74397465d473b9a7533722b7fba165c56c90cb3c2c4751795dbc3</originalsourceid><addsrcrecordid>eNptksFq3DAQhk1poCHJqS8g6KWh7FayZGvV23qTtoENgaY5C3ksb5Xa0lZjL9kn6-tFbgrJQqSDxMz3_8MMk2XvGZ1zruhnHFmRC1aWrHyTHedUshmjBX374v8uO0O8p-lwzhQrj7O_VQwAoXNkbc3OIvlYRYPowJDQ2WjAGrIzcU7cYLoUPScGiSG3YYxgSWhJ5YKBwe0sWYV-G0bfpLxvyOqX7RPfkWp0XeP8hlRdgN_4hdxsB9enxOXDECdp8OQOJ-Bi703SkOtUNZp_iclp7dpkvofOkiWiReytH06zo9Z0aM_-vyfZ3dfLn6vvs_XNt6vVcj2DfCGHmSiBsUUNoBorBVdSlEUjJK-VkQXnMs9r2daGlQUUJSgKNYcchCyYVEVTAz_JPjz5bmP4M1oc9H1q3aeSOldUKF4smHymNqaz2vk2TK31DkEvpVRciDT8RM1fodJtplEFb1uX4geC8wNBYgb7MGzMiKivbn8csp-eWIgBMdpWb2Mac9xrRvW0H_rFfvBHeOmr1g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2904935817</pqid></control><display><type>article</type><title>Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Manríquez-Zúñiga, Aranza Nallely ; de la Torre, Argelia Rosillo ; Valdés-Santiago, Laura ; Hernández-Bustos, Diana A ; Cuéllar-Sojo, Samantha ; Hernández-Rayas, Angélica ; Perez-Vega, Samuel ; Molina-Guerrero, Carlos Eduardo</creator><creatorcontrib>Manríquez-Zúñiga, Aranza Nallely ; de la Torre, Argelia Rosillo ; Valdés-Santiago, Laura ; Hernández-Bustos, Diana A ; Cuéllar-Sojo, Samantha ; Hernández-Rayas, Angélica ; Perez-Vega, Samuel ; Molina-Guerrero, Carlos Eduardo</creatorcontrib><description>Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovative products. In this research, we used and characterized dynamic maceration to extract BACs and CBBs from broccoli leaves (BLs). A central composite design (CCD) was selected to evaluate the effect of temperature (from 4 °C to 70 °C), ethanol concentration (from 30% to 70% (v/v)), and exposition time (15 to 60 min) on total phenolic content (TPC) (mg of gallic acid equivalents (GAEs) per 100 g of dry biomass (db)). A confirmation experiment (CE) was performed to reproduce the optimal conditions (50 °C, 36.92 min, and 30% (v/v)) for BAC extraction. Results indicated a GAE concentration of 112.95 ± 0.92 mg/100 g db, while the statistical model predicted a value of 111.87 mg of GAEs/100 g db (error of 0.95%) with a rate constant (k) value of 0.0154 mg/g·min (R2 of 0.9894). BACs and CBBs were identified with gas chromatography–electron impact mass spectrometry detecting l-isoleucine, l-leucine, malonic acid, and succinic acid, among others. Finally, a life cycle inventory (LCI) was developed to determine global warming (GW) and water consumption (WC), among others, for 10 g of BL extract. Findings reported herein prove the sustainability of eco-friendly extraction of BACs and CBBs for the effective use of agricultural by-products.</description><identifier>ISSN: 2071-1050</identifier><identifier>EISSN: 2071-1050</identifier><identifier>DOI: 10.3390/su152416616</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Amino acids ; Carotenoids ; Energy consumption ; Environmental impact ; Ethanol ; Global warming ; Mass spectrometry ; Organic chemicals ; Pharmaceutical industry ; Polyphenols ; Ratios ; Reproducibility ; Solvents ; Temperature ; Vegetable industry ; Vegetables</subject><ispartof>Sustainability, 2023-12, Vol.15 (24), p.16616</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c287t-46c118bcc9de74397465d473b9a7533722b7fba165c56c90cb3c2c4751795dbc3</cites><orcidid>0000-0001-5992-0379</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Manríquez-Zúñiga, Aranza Nallely</creatorcontrib><creatorcontrib>de la Torre, Argelia Rosillo</creatorcontrib><creatorcontrib>Valdés-Santiago, Laura</creatorcontrib><creatorcontrib>Hernández-Bustos, Diana A</creatorcontrib><creatorcontrib>Cuéllar-Sojo, Samantha</creatorcontrib><creatorcontrib>Hernández-Rayas, Angélica</creatorcontrib><creatorcontrib>Perez-Vega, Samuel</creatorcontrib><creatorcontrib>Molina-Guerrero, Carlos Eduardo</creatorcontrib><title>Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment</title><title>Sustainability</title><description>Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovative products. In this research, we used and characterized dynamic maceration to extract BACs and CBBs from broccoli leaves (BLs). A central composite design (CCD) was selected to evaluate the effect of temperature (from 4 °C to 70 °C), ethanol concentration (from 30% to 70% (v/v)), and exposition time (15 to 60 min) on total phenolic content (TPC) (mg of gallic acid equivalents (GAEs) per 100 g of dry biomass (db)). A confirmation experiment (CE) was performed to reproduce the optimal conditions (50 °C, 36.92 min, and 30% (v/v)) for BAC extraction. Results indicated a GAE concentration of 112.95 ± 0.92 mg/100 g db, while the statistical model predicted a value of 111.87 mg of GAEs/100 g db (error of 0.95%) with a rate constant (k) value of 0.0154 mg/g·min (R2 of 0.9894). BACs and CBBs were identified with gas chromatography–electron impact mass spectrometry detecting l-isoleucine, l-leucine, malonic acid, and succinic acid, among others. Finally, a life cycle inventory (LCI) was developed to determine global warming (GW) and water consumption (WC), among others, for 10 g of BL extract. Findings reported herein prove the sustainability of eco-friendly extraction of BACs and CBBs for the effective use of agricultural by-products.</description><subject>Amino acids</subject><subject>Carotenoids</subject><subject>Energy consumption</subject><subject>Environmental impact</subject><subject>Ethanol</subject><subject>Global warming</subject><subject>Mass spectrometry</subject><subject>Organic chemicals</subject><subject>Pharmaceutical industry</subject><subject>Polyphenols</subject><subject>Ratios</subject><subject>Reproducibility</subject><subject>Solvents</subject><subject>Temperature</subject><subject>Vegetable industry</subject><subject>Vegetables</subject><issn>2071-1050</issn><issn>2071-1050</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptksFq3DAQhk1poCHJqS8g6KWh7FayZGvV23qTtoENgaY5C3ksb5Xa0lZjL9kn6-tFbgrJQqSDxMz3_8MMk2XvGZ1zruhnHFmRC1aWrHyTHedUshmjBX374v8uO0O8p-lwzhQrj7O_VQwAoXNkbc3OIvlYRYPowJDQ2WjAGrIzcU7cYLoUPScGiSG3YYxgSWhJ5YKBwe0sWYV-G0bfpLxvyOqX7RPfkWp0XeP8hlRdgN_4hdxsB9enxOXDECdp8OQOJ-Bi703SkOtUNZp_iclp7dpkvofOkiWiReytH06zo9Z0aM_-vyfZ3dfLn6vvs_XNt6vVcj2DfCGHmSiBsUUNoBorBVdSlEUjJK-VkQXnMs9r2daGlQUUJSgKNYcchCyYVEVTAz_JPjz5bmP4M1oc9H1q3aeSOldUKF4smHymNqaz2vk2TK31DkEvpVRciDT8RM1fodJtplEFb1uX4geC8wNBYgb7MGzMiKivbn8csp-eWIgBMdpWb2Mac9xrRvW0H_rFfvBHeOmr1g</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Manríquez-Zúñiga, Aranza Nallely</creator><creator>de la Torre, Argelia Rosillo</creator><creator>Valdés-Santiago, Laura</creator><creator>Hernández-Bustos, Diana A</creator><creator>Cuéllar-Sojo, Samantha</creator><creator>Hernández-Rayas, Angélica</creator><creator>Perez-Vega, Samuel</creator><creator>Molina-Guerrero, Carlos Eduardo</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>4U-</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0001-5992-0379</orcidid></search><sort><creationdate>20231201</creationdate><title>Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment</title><author>Manríquez-Zúñiga, Aranza Nallely ; de la Torre, Argelia Rosillo ; Valdés-Santiago, Laura ; Hernández-Bustos, Diana A ; Cuéllar-Sojo, Samantha ; Hernández-Rayas, Angélica ; Perez-Vega, Samuel ; Molina-Guerrero, Carlos Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c287t-46c118bcc9de74397465d473b9a7533722b7fba165c56c90cb3c2c4751795dbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amino acids</topic><topic>Carotenoids</topic><topic>Energy consumption</topic><topic>Environmental impact</topic><topic>Ethanol</topic><topic>Global warming</topic><topic>Mass spectrometry</topic><topic>Organic chemicals</topic><topic>Pharmaceutical industry</topic><topic>Polyphenols</topic><topic>Ratios</topic><topic>Reproducibility</topic><topic>Solvents</topic><topic>Temperature</topic><topic>Vegetable industry</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manríquez-Zúñiga, Aranza Nallely</creatorcontrib><creatorcontrib>de la Torre, Argelia Rosillo</creatorcontrib><creatorcontrib>Valdés-Santiago, Laura</creatorcontrib><creatorcontrib>Hernández-Bustos, Diana A</creatorcontrib><creatorcontrib>Cuéllar-Sojo, Samantha</creatorcontrib><creatorcontrib>Hernández-Rayas, Angélica</creatorcontrib><creatorcontrib>Perez-Vega, Samuel</creatorcontrib><creatorcontrib>Molina-Guerrero, Carlos Eduardo</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>University Readers</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Sustainability</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manríquez-Zúñiga, Aranza Nallely</au><au>de la Torre, Argelia Rosillo</au><au>Valdés-Santiago, Laura</au><au>Hernández-Bustos, Diana A</au><au>Cuéllar-Sojo, Samantha</au><au>Hernández-Rayas, Angélica</au><au>Perez-Vega, Samuel</au><au>Molina-Guerrero, Carlos Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment</atitle><jtitle>Sustainability</jtitle><date>2023-12-01</date><risdate>2023</risdate><volume>15</volume><issue>24</issue><spage>16616</spage><pages>16616-</pages><issn>2071-1050</issn><eissn>2071-1050</eissn><abstract>Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovative products. In this research, we used and characterized dynamic maceration to extract BACs and CBBs from broccoli leaves (BLs). A central composite design (CCD) was selected to evaluate the effect of temperature (from 4 °C to 70 °C), ethanol concentration (from 30% to 70% (v/v)), and exposition time (15 to 60 min) on total phenolic content (TPC) (mg of gallic acid equivalents (GAEs) per 100 g of dry biomass (db)). A confirmation experiment (CE) was performed to reproduce the optimal conditions (50 °C, 36.92 min, and 30% (v/v)) for BAC extraction. Results indicated a GAE concentration of 112.95 ± 0.92 mg/100 g db, while the statistical model predicted a value of 111.87 mg of GAEs/100 g db (error of 0.95%) with a rate constant (k) value of 0.0154 mg/g·min (R2 of 0.9894). BACs and CBBs were identified with gas chromatography–electron impact mass spectrometry detecting l-isoleucine, l-leucine, malonic acid, and succinic acid, among others. Finally, a life cycle inventory (LCI) was developed to determine global warming (GW) and water consumption (WC), among others, for 10 g of BL extract. Findings reported herein prove the sustainability of eco-friendly extraction of BACs and CBBs for the effective use of agricultural by-products.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/su152416616</doi><orcidid>https://orcid.org/0000-0001-5992-0379</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2071-1050
ispartof Sustainability, 2023-12, Vol.15 (24), p.16616
issn 2071-1050
2071-1050
language eng
recordid cdi_proquest_journals_2904935817
source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Amino acids
Carotenoids
Energy consumption
Environmental impact
Ethanol
Global warming
Mass spectrometry
Organic chemicals
Pharmaceutical industry
Polyphenols
Ratios
Reproducibility
Solvents
Temperature
Vegetable industry
Vegetables
title Broccoli Leaves (Brassica oleracea var. italica) as a Source of Bioactive Compounds and Chemical Building Blocks: Optimal Extraction Using Dynamic Maceration and Life Cycle Assessment
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T14%3A17%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Broccoli%20Leaves%20(Brassica%20oleracea%20var.%20italica)%20as%20a%20Source%20of%20Bioactive%20Compounds%20and%20Chemical%20Building%20Blocks:%20Optimal%20Extraction%20Using%20Dynamic%20Maceration%20and%20Life%20Cycle%20Assessment&rft.jtitle=Sustainability&rft.au=Manr%C3%ADquez-Z%C3%BA%C3%B1iga,%20Aranza%20Nallely&rft.date=2023-12-01&rft.volume=15&rft.issue=24&rft.spage=16616&rft.pages=16616-&rft.issn=2071-1050&rft.eissn=2071-1050&rft_id=info:doi/10.3390/su152416616&rft_dat=%3Cgale_proqu%3EA779344050%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2904935817&rft_id=info:pmid/&rft_galeid=A779344050&rfr_iscdi=true