Leveraging Indian pulse proteins for plant‐based mock meat III: physicochemical characterisation, FTIR spectra and texture profile analysis of meat analogues

Summary The current study was designed to supersede the meat protein with pulse‐based proteins and to determine the suitability of the processing method for commercialisation of plant protein meat analogues. The pulse protein concentrates (PPCs) were extracted from green gram, horse gram and cowpea using alkaline/isoelectric precipitation method. The PPCs were subjected for physicochemical, morphological, GC–MS and thermal analysis. The PPCs of green gram to horse gram to cowpea were used in the ratio of (20:20:20) T1, (30:15:15) T2 and (15:20:15) T3 to prepare deep‐fried meatballs. All the PPCs exhibited collapsed and wrinkled surface. The horse gram protein concentrates exhibited the highest denaturation temperature (Td °C) 89.50 ± 2.57 and enthalpy (ΔH (J g−1)) (287.73 ± 9.64) iterating better thermal stability compared to other samples. FTIR spectra indicated the presence of O–H stretching wide bands for mutton deep‐fried meatballs (3321.22 cm−1) and plant‐based deep‐fried meatballs (3288.28 cm−1), whereas PPCs in the region of (3275–3278 cm−1). Two C‐H bands and the main secondary structural components such as α‐helix, β‐sheet, β‐turn and random coil of PPCs were observed in the region of 1600–1700 cm−1. Amide N–H bending (1400–1500 cm−1) and the C–O stretching bands (1000–1300 cm−1) were observed for all the samples. The plant‐based deep‐fried meatball formulated at the ratio of 20:20:20 (T1) was closely related to the mutton deep‐fried meatballs in terms of organoleptic properties (colour, texture, juiciness and overall acceptability), colour properties (L* and b*) and textural properties such as hardness, adhesiveness and cohesiveness. These findings will open new research horizons in this area and pave the way for the commercialisation of meat substitutes, which will reduce the environmental impact and carbon footprint. Production of pulse protein‐based meatball analogues.