Evaluation of the sealing ability of White Mineral Trioxide Aggregate, Biodentine and Mineral Trioxide Aggregate Repair HP as root end filling materials with two different Retro-cavity preparation techniques. An in?vitro Ultraviolet-Spectrophotometric analysis

This study aims to evaluate sealing ability of White Mineral Trioxide Aggregate, Biodentine and Mineral Trioxide Aggregate Repair HP as root end filling materials with two different Retro-cavity preparation techniques by in-vitro Ultraviolet-Spectrophotometric analysis.140 extracted human mandibular single rooted premolar teeth were included.In all specimens, Endodontic access cavity preparations were done, pulp tissue was extirpated and working length was established. Biomechanical preparation was done in step-back technique with K?file no.40 as master apical file along with the use of root canal irrigants.Root canals were then dried, coated with AH-plus sealer, obturated with guttapercha by cold lateral condensation technique and access cavities were restored with Glass ionomer cement. In all the specimens, the apical 3mm of roots were resected and were then divided into 2 groups; Experimental group with 120 specimens and Control group with 20 specimens.Experimental group is divided into Group-I and Group-II with 60 specimens each. 3mm depth of retro-cavity preparations were done. In Group-I, Class-1 Retro-cavity preparation was done using diamond bur and in Group-II, Retro-cavity preparation was done using Pro-ultrasonic tip. Group-I and II were further divided into three groups each with 20 specimens per group.In Group-IA and IIA; White MTA, Group-IB and IIB; Biodentine, Group-IC and IIC; MTA Repair HP were used as root end filling materials.All specimens were immersed in Indian ink dye for 48hours and were then subjected to dye extraction method, obtained solutions were analysed using Ultraviolet-Spectrophotometer and readings were recorded as absorbanceunits. Obtained data was statistically analysed using One-way ANOVA andTukey’s post-hoc tests.Retro-cavity preparation using ultrasonic tip with biodentine as root end filling material showed the maximum sealing ability. However, there was no significant difference in the sealing ability between Biodentine and MTA Repair HP as root end filling material.