A protocol for in vitro propagation of Morella pubescens: a protected species in the Tambillo community protected area—Ecuador
A protocol was developed for the in vitro propagation of Morella pubescens (Humb. & Bonpl. ex Willd.) Wilbur, a species poorly investigated and not previously reported, with the purpose of massively obtaining plants and meeting the goal of reforesting Tambillo, the first protected community area...
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container_title | Plant cell, tissue and organ culture |
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description | A protocol was developed for the in vitro propagation of
Morella pubescens
(Humb. & Bonpl. ex Willd.) Wilbur, a species poorly investigated and not previously reported, with the purpose of massively obtaining plants and meeting the goal of reforesting Tambillo, the first protected community area in Ecuador. Apexes of
M. pubescens
as explants, which were aseptically disinfected with a sodium hypochlorite solution, were subcultured in WPM basal medium, supplemented with GA
3
and BAP to determine the number of leaves and shoots. Subsequently, the explants were incubated in WPM supplemented with IBA and IAA and the number and length of roots were evaluated. A completely randomized design was used with five and seven replicates, respectively. And for
ex vitro
acclimatization, the survival of 200 vitroplants was evaluated. The best response in multiplication was observed in WPM with 0.25 mg L
−1
GA
3
+ 1.5 mg L
−1
BAP. The number and length of roots per explant was higher in the treatment with 1 mg L
−1
IBA and IAA. Out of 200 vitroplants, 65% survived the environmental conditions. The proper selection of the concentration and type of the growth phytoregulator and the appropriate environmental conditions are important factors to micropropagate
Morella pubescens
.
Key message
The successful development of the protocol for the in vitro propagation of
Morella pubescens
a protected species in Ecuador, establishes the foundations for mass plant production and reforestation in the area. |
doi_str_mv | 10.1007/s11240-023-02643-y |
format | Article |
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Morella pubescens
(Humb. & Bonpl. ex Willd.) Wilbur, a species poorly investigated and not previously reported, with the purpose of massively obtaining plants and meeting the goal of reforesting Tambillo, the first protected community area in Ecuador. Apexes of
M. pubescens
as explants, which were aseptically disinfected with a sodium hypochlorite solution, were subcultured in WPM basal medium, supplemented with GA
3
and BAP to determine the number of leaves and shoots. Subsequently, the explants were incubated in WPM supplemented with IBA and IAA and the number and length of roots were evaluated. A completely randomized design was used with five and seven replicates, respectively. And for
ex vitro
acclimatization, the survival of 200 vitroplants was evaluated. The best response in multiplication was observed in WPM with 0.25 mg L
−1
GA
3
+ 1.5 mg L
−1
BAP. The number and length of roots per explant was higher in the treatment with 1 mg L
−1
IBA and IAA. Out of 200 vitroplants, 65% survived the environmental conditions. The proper selection of the concentration and type of the growth phytoregulator and the appropriate environmental conditions are important factors to micropropagate
Morella pubescens
.
Key message
The successful development of the protocol for the in vitro propagation of
Morella pubescens
a protected species in Ecuador, establishes the foundations for mass plant production and reforestation in the area.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02643-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acclimatization ; Biomedical and Life Sciences ; Disinfectants ; Environmental conditions ; Explants ; Life Sciences ; Morella pubescens ; Original Article ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant production ; Plant propagation ; Plant Sciences ; Propagation ; Protected areas ; Protected species ; Reforestation ; Roots ; Sodium hypochlorite</subject><ispartof>Plant cell, tissue and organ culture, 2024, Vol.156 (1), p.20, Article 20</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-e83d91cc1a10a51c9bc3da5849db8e4ed544712ac55ef03c3e2a809f38c57753</cites><orcidid>0000-0002-3619-9598</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02643-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02643-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Borrero, Kevin Brayan Murillo</creatorcontrib><creatorcontrib>Corozo-Quiñónez, Liliana</creatorcontrib><creatorcontrib>Durán, Mateo León</creatorcontrib><creatorcontrib>Ponce, Fátima Macías</creatorcontrib><creatorcontrib>Pinoargote, Miryan</creatorcontrib><creatorcontrib>Saltos-Rezabala, Luis Alberto</creatorcontrib><title>A protocol for in vitro propagation of Morella pubescens: a protected species in the Tambillo community protected area—Ecuador</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>A protocol was developed for the in vitro propagation of
Morella pubescens
(Humb. & Bonpl. ex Willd.) Wilbur, a species poorly investigated and not previously reported, with the purpose of massively obtaining plants and meeting the goal of reforesting Tambillo, the first protected community area in Ecuador. Apexes of
M. pubescens
as explants, which were aseptically disinfected with a sodium hypochlorite solution, were subcultured in WPM basal medium, supplemented with GA
3
and BAP to determine the number of leaves and shoots. Subsequently, the explants were incubated in WPM supplemented with IBA and IAA and the number and length of roots were evaluated. A completely randomized design was used with five and seven replicates, respectively. And for
ex vitro
acclimatization, the survival of 200 vitroplants was evaluated. The best response in multiplication was observed in WPM with 0.25 mg L
−1
GA
3
+ 1.5 mg L
−1
BAP. The number and length of roots per explant was higher in the treatment with 1 mg L
−1
IBA and IAA. Out of 200 vitroplants, 65% survived the environmental conditions. The proper selection of the concentration and type of the growth phytoregulator and the appropriate environmental conditions are important factors to micropropagate
Morella pubescens
.
Key message
The successful development of the protocol for the in vitro propagation of
Morella pubescens
a protected species in Ecuador, establishes the foundations for mass plant production and reforestation in the area.</description><subject>Acclimatization</subject><subject>Biomedical and Life Sciences</subject><subject>Disinfectants</subject><subject>Environmental conditions</subject><subject>Explants</subject><subject>Life Sciences</subject><subject>Morella pubescens</subject><subject>Original Article</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant production</subject><subject>Plant propagation</subject><subject>Plant Sciences</subject><subject>Propagation</subject><subject>Protected areas</subject><subject>Protected species</subject><subject>Reforestation</subject><subject>Roots</subject><subject>Sodium hypochlorite</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kM1Kw0AUhQdRsFZfwNWA6-j85sddKfUHKm66HyaTm5qSZOJMImTXh_AJfRKnjaArF4cLl_OdezkIXVNySwlJ7jylTJCIMB4UCx6NJ2hGZcIjSYQ4RTNC4ySKU5mcowvvd4SQmAs6Q_sF7pztrbE1Lq3DVYs_qt7Zw7bTW91XtsW2xC_WQV1r3A05eAOtv8f6SILpocC-A1OBP-D9G-CNbvKqri02tmmGturHP17tQH_tP1dm0IV1l-is1LWHq585R5uH1Wb5FK1fH5-Xi3VkWEL6CFJeZNQYqinRkposN7zQMhVZkacgoJBCJJRpIyWUhBsOTKckK3lqZJJIPkc3U2z4430A36udHVwbLiqWERrEmAguNrmMs947KFXnqka7UVGiDkWrqWgVilbHotUYID5BPpjbLbjf6H-ob01IhII</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Borrero, Kevin Brayan Murillo</creator><creator>Corozo-Quiñónez, Liliana</creator><creator>Durán, Mateo León</creator><creator>Ponce, Fátima Macías</creator><creator>Pinoargote, Miryan</creator><creator>Saltos-Rezabala, Luis Alberto</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3619-9598</orcidid></search><sort><creationdate>2024</creationdate><title>A protocol for in vitro propagation of Morella pubescens: a protected species in the Tambillo community protected area—Ecuador</title><author>Borrero, Kevin Brayan Murillo ; Corozo-Quiñónez, Liliana ; Durán, Mateo León ; Ponce, Fátima Macías ; Pinoargote, Miryan ; Saltos-Rezabala, Luis Alberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-e83d91cc1a10a51c9bc3da5849db8e4ed544712ac55ef03c3e2a809f38c57753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acclimatization</topic><topic>Biomedical and Life Sciences</topic><topic>Disinfectants</topic><topic>Environmental conditions</topic><topic>Explants</topic><topic>Life Sciences</topic><topic>Morella pubescens</topic><topic>Original Article</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant production</topic><topic>Plant propagation</topic><topic>Plant Sciences</topic><topic>Propagation</topic><topic>Protected areas</topic><topic>Protected species</topic><topic>Reforestation</topic><topic>Roots</topic><topic>Sodium hypochlorite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borrero, Kevin Brayan Murillo</creatorcontrib><creatorcontrib>Corozo-Quiñónez, Liliana</creatorcontrib><creatorcontrib>Durán, Mateo León</creatorcontrib><creatorcontrib>Ponce, Fátima Macías</creatorcontrib><creatorcontrib>Pinoargote, Miryan</creatorcontrib><creatorcontrib>Saltos-Rezabala, Luis Alberto</creatorcontrib><collection>CrossRef</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borrero, Kevin Brayan Murillo</au><au>Corozo-Quiñónez, Liliana</au><au>Durán, Mateo León</au><au>Ponce, Fátima Macías</au><au>Pinoargote, Miryan</au><au>Saltos-Rezabala, Luis Alberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A protocol for in vitro propagation of Morella pubescens: a protected species in the Tambillo community protected area—Ecuador</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2024</date><risdate>2024</risdate><volume>156</volume><issue>1</issue><spage>20</spage><pages>20-</pages><artnum>20</artnum><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>A protocol was developed for the in vitro propagation of
Morella pubescens
(Humb. & Bonpl. ex Willd.) Wilbur, a species poorly investigated and not previously reported, with the purpose of massively obtaining plants and meeting the goal of reforesting Tambillo, the first protected community area in Ecuador. Apexes of
M. pubescens
as explants, which were aseptically disinfected with a sodium hypochlorite solution, were subcultured in WPM basal medium, supplemented with GA
3
and BAP to determine the number of leaves and shoots. Subsequently, the explants were incubated in WPM supplemented with IBA and IAA and the number and length of roots were evaluated. A completely randomized design was used with five and seven replicates, respectively. And for
ex vitro
acclimatization, the survival of 200 vitroplants was evaluated. The best response in multiplication was observed in WPM with 0.25 mg L
−1
GA
3
+ 1.5 mg L
−1
BAP. The number and length of roots per explant was higher in the treatment with 1 mg L
−1
IBA and IAA. Out of 200 vitroplants, 65% survived the environmental conditions. The proper selection of the concentration and type of the growth phytoregulator and the appropriate environmental conditions are important factors to micropropagate
Morella pubescens
.
Key message
The successful development of the protocol for the in vitro propagation of
Morella pubescens
a protected species in Ecuador, establishes the foundations for mass plant production and reforestation in the area.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02643-y</doi><orcidid>https://orcid.org/0000-0002-3619-9598</orcidid></addata></record> |
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source | SpringerNature Journals |
subjects | Acclimatization Biomedical and Life Sciences Disinfectants Environmental conditions Explants Life Sciences Morella pubescens Original Article Plant Genetics and Genomics Plant Pathology Plant Physiology Plant production Plant propagation Plant Sciences Propagation Protected areas Protected species Reforestation Roots Sodium hypochlorite |
title | A protocol for in vitro propagation of Morella pubescens: a protected species in the Tambillo community protected area—Ecuador |
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