Overexpression of BnNAC19 in Brassica napus enhances resistance to Leptosphaeria maculans, the blackleg pathogen of canola
Leptosphaeria maculans is a fungal pathogen that causes blackleg disease in canola (Brassica napus), resulting in significant yield and economic losses in Canada and many parts of the world. Plant NAC transcription factors play critical roles in plant development and response to biotic or abiotic st...
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| Veröffentlicht in: | Plant pathology 2024-01, Vol.73 (1), p.104-114 |
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| creator | Zou, Zhongwei Fernando, W. G. Dilantha |
| description | Leptosphaeria maculans is a fungal pathogen that causes blackleg disease in canola (Brassica napus), resulting in significant yield and economic losses in Canada and many parts of the world. Plant NAC transcription factors play critical roles in plant development and response to biotic or abiotic stress. In this study, we identified and characterized a BnNAC19 gene from Brassica napus. The overexpression of BnNAC19 in transgenic canola plants contributed to the improvement of seedling resistance against L. maculans. The mycelial growth of a green fluorescent protein‐tagged strain of L. maculans and production of pycnidiospores were shown to be inhibited in the transgenic canola plants overexpressing BnNAC19. In addition, the canola transgenic line overexpressing BnNAC19 showed increased disease resistance in the adult plant, which was determined by quantitative resistance. Both increased seedling and adult plant resistance in transgenic canola plants overexpressing BnNAC19 indicate that the BnNAC19 gene plays a positive role against L. maculans. The expression pattern of genes BnNAC19 upstream and downstream of BnNAC19 that participate in plant defence pathways were investigated to elucidate the B. napus resistance mechanisms to L. maculans infection, and hence to aid the long‐term blackleg disease‐resistant breeding programmes.
Overexpression of BnNAC19 in canola (Brassica napus) can enhance disease resistance against blackleg, caused by Leptosphaeria maculans, providing a valuable genetic resource for disease breeding. |
| doi_str_mv | 10.1111/ppa.13791 |
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Overexpression of BnNAC19 in canola (Brassica napus) can enhance disease resistance against blackleg, caused by Leptosphaeria maculans, providing a valuable genetic resource for disease breeding.</description><subject>Blackleg</subject><subject>BnNAC19</subject><subject>Brassica</subject><subject>Brassica napus</subject><subject>Canola</subject><subject>Disease resistance</subject><subject>Economic impact</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Green fluorescent protein</subject><subject>Leptosphaeria maculans</subject><subject>overexpression</subject><subject>Pathogens</subject><subject>Plant resistance</subject><subject>Pycnidiospores</subject><subject>Rape plants</subject><subject>resistance</subject><subject>Seedlings</subject><subject>Transcription factors</subject><subject>Transgenic plants</subject><issn>0032-0862</issn><issn>1365-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNp1kE1OwzAQRi0EEqWw4AaWWCGR1o7TNF6Wij-pol3A2po6kyYltY2dABWX4SycjJSyZTYzGr2ZT3qEnHM24F0NnYMBF2PJD0iPi3QUCTaSh6THmIgjlqXxMTkJYc0YH0mZ9cjn_A09fjiPIVTWUFvQa_M4mXJJK0OvPXRrDdSAawNFU4LRGGhHV6HZzbSxdIauscGVgL4CugHd1mDCFW1KpMsa9EuNK-qgKe0KdwnfXxqMreGUHBVQBzz7633yfHvzNL2PZvO7h-lkFmkhYh7lWOSYJILjOGcskVxrDhAnIIucQSxzPV5yngEvEilTkYiMs7xYppkQCAIT0ScX-7_O29cWQ6PWtvWmi1SxZCxNpYjTjrrcU9rbEDwWyvlqA36rOFM7t6pzq37dduxwz75XNW7_B9ViMdlf_AC8bH0J</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Zou, Zhongwei</creator><creator>Fernando, W. G. Dilantha</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-8955-1153</orcidid><orcidid>https://orcid.org/0000-0002-2839-1539</orcidid></search><sort><creationdate>202401</creationdate><title>Overexpression of BnNAC19 in Brassica napus enhances resistance to Leptosphaeria maculans, the blackleg pathogen of canola</title><author>Zou, Zhongwei ; Fernando, W. G. Dilantha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3321-defde4431e7d00491cc1aa24a9fd0a29dc7b118a1f4996343810dfb6833ea3e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Blackleg</topic><topic>BnNAC19</topic><topic>Brassica</topic><topic>Brassica napus</topic><topic>Canola</topic><topic>Disease resistance</topic><topic>Economic impact</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Green fluorescent protein</topic><topic>Leptosphaeria maculans</topic><topic>overexpression</topic><topic>Pathogens</topic><topic>Plant resistance</topic><topic>Pycnidiospores</topic><topic>Rape plants</topic><topic>resistance</topic><topic>Seedlings</topic><topic>Transcription factors</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, Zhongwei</creatorcontrib><creatorcontrib>Fernando, W. G. Dilantha</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Free Content</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, Zhongwei</au><au>Fernando, W. G. Dilantha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of BnNAC19 in Brassica napus enhances resistance to Leptosphaeria maculans, the blackleg pathogen of canola</atitle><jtitle>Plant pathology</jtitle><date>2024-01</date><risdate>2024</risdate><volume>73</volume><issue>1</issue><spage>104</spage><epage>114</epage><pages>104-114</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><abstract>Leptosphaeria maculans is a fungal pathogen that causes blackleg disease in canola (Brassica napus), resulting in significant yield and economic losses in Canada and many parts of the world. Plant NAC transcription factors play critical roles in plant development and response to biotic or abiotic stress. In this study, we identified and characterized a BnNAC19 gene from Brassica napus. The overexpression of BnNAC19 in transgenic canola plants contributed to the improvement of seedling resistance against L. maculans. The mycelial growth of a green fluorescent protein‐tagged strain of L. maculans and production of pycnidiospores were shown to be inhibited in the transgenic canola plants overexpressing BnNAC19. In addition, the canola transgenic line overexpressing BnNAC19 showed increased disease resistance in the adult plant, which was determined by quantitative resistance. Both increased seedling and adult plant resistance in transgenic canola plants overexpressing BnNAC19 indicate that the BnNAC19 gene plays a positive role against L. maculans. The expression pattern of genes BnNAC19 upstream and downstream of BnNAC19 that participate in plant defence pathways were investigated to elucidate the B. napus resistance mechanisms to L. maculans infection, and hence to aid the long‐term blackleg disease‐resistant breeding programmes.
Overexpression of BnNAC19 in canola (Brassica napus) can enhance disease resistance against blackleg, caused by Leptosphaeria maculans, providing a valuable genetic resource for disease breeding.</abstract><cop>Oxford</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/ppa.13791</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8955-1153</orcidid><orcidid>https://orcid.org/0000-0002-2839-1539</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Blackleg BnNAC19 Brassica Brassica napus Canola Disease resistance Economic impact Fluorescence Gene expression Green fluorescent protein Leptosphaeria maculans overexpression Pathogens Plant resistance Pycnidiospores Rape plants resistance Seedlings Transcription factors Transgenic plants |
| title | Overexpression of BnNAC19 in Brassica napus enhances resistance to Leptosphaeria maculans, the blackleg pathogen of canola |
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