Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels
In this study we investigated the role of voltage dependent (K V ), Ca 2+ -activated (K Ca ), and inward rectifier (K ir ) potassium channels in the effects of hydrogen sulfide donor (H 2 S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-de...
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| Veröffentlicht in: | Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2023-12, Vol.17 (4), p.301-310 |
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| container_title | Biochemistry (Moscow). Supplement series A, Membrane and cell biology |
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| creator | Sorokina, D. M. Shaidullov, I. F. Buchareb, D. Sitdikov, F. G. Sitdikova, G. F. |
| description | In this study we investigated the role of voltage dependent (K
V
), Ca
2+
-activated (K
Ca
), and inward rectifier (K
ir
) potassium channels in the effects of hydrogen sulfide donor (H
2
S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-dependently (10–500 μM) reduced the tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions of jejunum preparations under isometric conditions; the half-maximal effective concentration (EC
50
) of the inhibitory effect of NaHS on the amplitude of contractions was 165 μM. The blocker of K
V
channels 4-AP (200 μM) caused an increase in the amplitude of spontaneous contractions. NaHS (200 μM) decreased the amplitude and frequency of spontaneous activity of the preparation in the presence of 4-AP as well as in the control, and the effect on basal tonus was less pronounced. Blockers of large conductance K
Ca
channels (BK), non-specific TEA (3 mM) and specific paxillin (1 μM), increased the amplitude of spontaneous contractions, while the depressing effect of NaHS was completely preserved. The selective blocker of small conductance K
Ca
channels (SK) NS8593 (4 μM) did not affect the tonus of the preparation and the parameters of spontaneous contractions; it did not prevent the effect of NaHS. The activator of K
ATP
channels diazoxide (100 μM) caused a decrease in the basal tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions. Diazoxide and the K
ATP
channel blocker glibenclamide (50 μM) prevented the effect of NaHS on the tonus of the preparation. BaCl
2
, the K
ir
channel blocker (30 μM), caused an increase in the amplitude of spontaneous contractions and prevented the development of the NaHS inhibitory effects on the frequency and amplitude of spontaneous contractions; the decrease in tonus was less pronounced than in the control. Thus, a decrease in the basal tonus of the rat jejunum preparation under the action of the H
2
S donor was associated with activation of K
ir
channels, including K
ATP
channels, whereas the effect of H
2
S on amplitude and frequency was mediated by an increase in Ba
2+
-sensitive conductivity. |
| doi_str_mv | 10.1134/S1990747823060089 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2900318781</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2900318781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c268t-30abd9fe14edf36f49d3c7cf5159b02cac76be42af18650315ef28f01fd4cbd43</originalsourceid><addsrcrecordid>eNp1kF9LwzAUxYMoOKcfwLeAr5smTZomvkmZTjcQNvW1pPmzdXRJTdqHfXtbJvogPt3Dved3LhwArjG6xZjQuzUWAmU04wlBDCEuTsBoWE0zKujpj874ObiIcYcQI5SxEdjPrDWqhd7C-UEHvzEOrru62VbaQN_rxrtWOuO7CPNeBqnayrs4AO3WwJVs4YvZda7b38OVr81wWHxM4CKXEyidhosqwHwrnTN1vARnVtbRXH3PMXh_nL3l8-ny9ek5f1hOVcJ4OyVIllpYg6nRljBLhSYqUzbFqShRoqTKWGloIi3mLEUEp8Ym3CJsNVWlpmQMbo65TfCfnYltsfNdcP3LIhGoB3jGce_CR5cKPsZgbNGEai_DocCoGFot_rTaM8mRib3XbUz4Tf4f-gLw-XjB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2900318781</pqid></control><display><type>article</type><title>Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels</title><source>SpringerNature Journals</source><creator>Sorokina, D. M. ; Shaidullov, I. F. ; Buchareb, D. ; Sitdikov, F. G. ; Sitdikova, G. F.</creator><creatorcontrib>Sorokina, D. M. ; Shaidullov, I. F. ; Buchareb, D. ; Sitdikov, F. G. ; Sitdikova, G. F.</creatorcontrib><description>In this study we investigated the role of voltage dependent (K
V
), Ca
2+
-activated (K
Ca
), and inward rectifier (K
ir
) potassium channels in the effects of hydrogen sulfide donor (H
2
S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-dependently (10–500 μM) reduced the tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions of jejunum preparations under isometric conditions; the half-maximal effective concentration (EC
50
) of the inhibitory effect of NaHS on the amplitude of contractions was 165 μM. The blocker of K
V
channels 4-AP (200 μM) caused an increase in the amplitude of spontaneous contractions. NaHS (200 μM) decreased the amplitude and frequency of spontaneous activity of the preparation in the presence of 4-AP as well as in the control, and the effect on basal tonus was less pronounced. Blockers of large conductance K
Ca
channels (BK), non-specific TEA (3 mM) and specific paxillin (1 μM), increased the amplitude of spontaneous contractions, while the depressing effect of NaHS was completely preserved. The selective blocker of small conductance K
Ca
channels (SK) NS8593 (4 μM) did not affect the tonus of the preparation and the parameters of spontaneous contractions; it did not prevent the effect of NaHS. The activator of K
ATP
channels diazoxide (100 μM) caused a decrease in the basal tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions. Diazoxide and the K
ATP
channel blocker glibenclamide (50 μM) prevented the effect of NaHS on the tonus of the preparation. BaCl
2
, the K
ir
channel blocker (30 μM), caused an increase in the amplitude of spontaneous contractions and prevented the development of the NaHS inhibitory effects on the frequency and amplitude of spontaneous contractions; the decrease in tonus was less pronounced than in the control. Thus, a decrease in the basal tonus of the rat jejunum preparation under the action of the H
2
S donor was associated with activation of K
ir
channels, including K
ATP
channels, whereas the effect of H
2
S on amplitude and frequency was mediated by an increase in Ba
2+
-sensitive conductivity.</description><identifier>ISSN: 1990-7478</identifier><identifier>EISSN: 1990-7494</identifier><identifier>DOI: 10.1134/S1990747823060089</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Amplitudes ; Barium chloride ; Biomedical and Life Sciences ; Calcium channels (voltage-gated) ; Calcium ions ; Cell Biology ; Channels ; Contractility ; Glibenclamide ; Hydrogen sulfide ; Isometric ; Jejunum ; Life Sciences ; Paxillin ; Potassium channels (calcium-gated) ; Potassium channels (inwardly-rectifying) ; Potassium channels (voltage-gated) ; Small intestine</subject><ispartof>Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2023-12, Vol.17 (4), p.301-310</ispartof><rights>Pleiades Publishing, Ltd. 2023. ISSN 1990-7478, Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2023, Vol. 17, No. 4, pp. 301–310. © Pleiades Publishing, Ltd., 2023. Russian Text © The Author(s), 2023, published in Biologicheskie Membrany, 2023, Vol. 40, No. 6, pp. 432–442.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-30abd9fe14edf36f49d3c7cf5159b02cac76be42af18650315ef28f01fd4cbd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1990747823060089$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1990747823060089$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Sorokina, D. M.</creatorcontrib><creatorcontrib>Shaidullov, I. F.</creatorcontrib><creatorcontrib>Buchareb, D.</creatorcontrib><creatorcontrib>Sitdikov, F. G.</creatorcontrib><creatorcontrib>Sitdikova, G. F.</creatorcontrib><title>Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels</title><title>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</title><addtitle>Biochem. Moscow Suppl. Ser. A</addtitle><description>In this study we investigated the role of voltage dependent (K
V
), Ca
2+
-activated (K
Ca
), and inward rectifier (K
ir
) potassium channels in the effects of hydrogen sulfide donor (H
2
S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-dependently (10–500 μM) reduced the tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions of jejunum preparations under isometric conditions; the half-maximal effective concentration (EC
50
) of the inhibitory effect of NaHS on the amplitude of contractions was 165 μM. The blocker of K
V
channels 4-AP (200 μM) caused an increase in the amplitude of spontaneous contractions. NaHS (200 μM) decreased the amplitude and frequency of spontaneous activity of the preparation in the presence of 4-AP as well as in the control, and the effect on basal tonus was less pronounced. Blockers of large conductance K
Ca
channels (BK), non-specific TEA (3 mM) and specific paxillin (1 μM), increased the amplitude of spontaneous contractions, while the depressing effect of NaHS was completely preserved. The selective blocker of small conductance K
Ca
channels (SK) NS8593 (4 μM) did not affect the tonus of the preparation and the parameters of spontaneous contractions; it did not prevent the effect of NaHS. The activator of K
ATP
channels diazoxide (100 μM) caused a decrease in the basal tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions. Diazoxide and the K
ATP
channel blocker glibenclamide (50 μM) prevented the effect of NaHS on the tonus of the preparation. BaCl
2
, the K
ir
channel blocker (30 μM), caused an increase in the amplitude of spontaneous contractions and prevented the development of the NaHS inhibitory effects on the frequency and amplitude of spontaneous contractions; the decrease in tonus was less pronounced than in the control. Thus, a decrease in the basal tonus of the rat jejunum preparation under the action of the H
2
S donor was associated with activation of K
ir
channels, including K
ATP
channels, whereas the effect of H
2
S on amplitude and frequency was mediated by an increase in Ba
2+
-sensitive conductivity.</description><subject>Amplitudes</subject><subject>Barium chloride</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium channels (voltage-gated)</subject><subject>Calcium ions</subject><subject>Cell Biology</subject><subject>Channels</subject><subject>Contractility</subject><subject>Glibenclamide</subject><subject>Hydrogen sulfide</subject><subject>Isometric</subject><subject>Jejunum</subject><subject>Life Sciences</subject><subject>Paxillin</subject><subject>Potassium channels (calcium-gated)</subject><subject>Potassium channels (inwardly-rectifying)</subject><subject>Potassium channels (voltage-gated)</subject><subject>Small intestine</subject><issn>1990-7478</issn><issn>1990-7494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kF9LwzAUxYMoOKcfwLeAr5smTZomvkmZTjcQNvW1pPmzdXRJTdqHfXtbJvogPt3Dved3LhwArjG6xZjQuzUWAmU04wlBDCEuTsBoWE0zKujpj874ObiIcYcQI5SxEdjPrDWqhd7C-UEHvzEOrru62VbaQN_rxrtWOuO7CPNeBqnayrs4AO3WwJVs4YvZda7b38OVr81wWHxM4CKXEyidhosqwHwrnTN1vARnVtbRXH3PMXh_nL3l8-ny9ek5f1hOVcJ4OyVIllpYg6nRljBLhSYqUzbFqShRoqTKWGloIi3mLEUEp8Ym3CJsNVWlpmQMbo65TfCfnYltsfNdcP3LIhGoB3jGce_CR5cKPsZgbNGEai_DocCoGFot_rTaM8mRib3XbUz4Tf4f-gLw-XjB</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Sorokina, D. M.</creator><creator>Shaidullov, I. F.</creator><creator>Buchareb, D.</creator><creator>Sitdikov, F. G.</creator><creator>Sitdikova, G. F.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20231201</creationdate><title>Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels</title><author>Sorokina, D. M. ; Shaidullov, I. F. ; Buchareb, D. ; Sitdikov, F. G. ; Sitdikova, G. F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-30abd9fe14edf36f49d3c7cf5159b02cac76be42af18650315ef28f01fd4cbd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amplitudes</topic><topic>Barium chloride</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium channels (voltage-gated)</topic><topic>Calcium ions</topic><topic>Cell Biology</topic><topic>Channels</topic><topic>Contractility</topic><topic>Glibenclamide</topic><topic>Hydrogen sulfide</topic><topic>Isometric</topic><topic>Jejunum</topic><topic>Life Sciences</topic><topic>Paxillin</topic><topic>Potassium channels (calcium-gated)</topic><topic>Potassium channels (inwardly-rectifying)</topic><topic>Potassium channels (voltage-gated)</topic><topic>Small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorokina, D. M.</creatorcontrib><creatorcontrib>Shaidullov, I. F.</creatorcontrib><creatorcontrib>Buchareb, D.</creatorcontrib><creatorcontrib>Sitdikov, F. G.</creatorcontrib><creatorcontrib>Sitdikova, G. F.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorokina, D. M.</au><au>Shaidullov, I. F.</au><au>Buchareb, D.</au><au>Sitdikov, F. G.</au><au>Sitdikova, G. F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels</atitle><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle><stitle>Biochem. Moscow Suppl. Ser. A</stitle><date>2023-12-01</date><risdate>2023</risdate><volume>17</volume><issue>4</issue><spage>301</spage><epage>310</epage><pages>301-310</pages><issn>1990-7478</issn><eissn>1990-7494</eissn><abstract>In this study we investigated the role of voltage dependent (K
V
), Ca
2+
-activated (K
Ca
), and inward rectifier (K
ir
) potassium channels in the effects of hydrogen sulfide donor (H
2
S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-dependently (10–500 μM) reduced the tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions of jejunum preparations under isometric conditions; the half-maximal effective concentration (EC
50
) of the inhibitory effect of NaHS on the amplitude of contractions was 165 μM. The blocker of K
V
channels 4-AP (200 μM) caused an increase in the amplitude of spontaneous contractions. NaHS (200 μM) decreased the amplitude and frequency of spontaneous activity of the preparation in the presence of 4-AP as well as in the control, and the effect on basal tonus was less pronounced. Blockers of large conductance K
Ca
channels (BK), non-specific TEA (3 mM) and specific paxillin (1 μM), increased the amplitude of spontaneous contractions, while the depressing effect of NaHS was completely preserved. The selective blocker of small conductance K
Ca
channels (SK) NS8593 (4 μM) did not affect the tonus of the preparation and the parameters of spontaneous contractions; it did not prevent the effect of NaHS. The activator of K
ATP
channels diazoxide (100 μM) caused a decrease in the basal tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions. Diazoxide and the K
ATP
channel blocker glibenclamide (50 μM) prevented the effect of NaHS on the tonus of the preparation. BaCl
2
, the K
ir
channel blocker (30 μM), caused an increase in the amplitude of spontaneous contractions and prevented the development of the NaHS inhibitory effects on the frequency and amplitude of spontaneous contractions; the decrease in tonus was less pronounced than in the control. Thus, a decrease in the basal tonus of the rat jejunum preparation under the action of the H
2
S donor was associated with activation of K
ir
channels, including K
ATP
channels, whereas the effect of H
2
S on amplitude and frequency was mediated by an increase in Ba
2+
-sensitive conductivity.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1990747823060089</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1990-7478 |
| ispartof | Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2023-12, Vol.17 (4), p.301-310 |
| issn | 1990-7478 1990-7494 |
| language | eng |
| recordid | cdi_proquest_journals_2900318781 |
| source | SpringerNature Journals |
| subjects | Amplitudes Barium chloride Biomedical and Life Sciences Calcium channels (voltage-gated) Calcium ions Cell Biology Channels Contractility Glibenclamide Hydrogen sulfide Isometric Jejunum Life Sciences Paxillin Potassium channels (calcium-gated) Potassium channels (inwardly-rectifying) Potassium channels (voltage-gated) Small intestine |
| title | Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels |
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