Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS
In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by...
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| Veröffentlicht in: | Electronics and communications in Japan 2023-12, Vol.106 (4), p.n/a |
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| creator | Miyairi, Akitsugu Hasegawa, Yuki Uchida, Hidekazu |
| description | In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration. |
| doi_str_mv | 10.1002/ecj.12425 |
| format | Article |
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Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.</description><identifier>ISSN: 1942-9533</identifier><identifier>EISSN: 1942-9541</identifier><identifier>DOI: 10.1002/ecj.12425</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc</publisher><subject>acetylcholinesterase ; Chemical sensors ; drug screening ; Electrical measurement ; electrochemical imaging sensor ; Electrodes ; LAAS ; micro array ; Schottky junction ; Substrates</subject><ispartof>Electronics and communications in Japan, 2023-12, Vol.106 (4), p.n/a</ispartof><rights>2023 Wiley Periodicals LLC.</rights><rights>2023 by Wiley Periodicals LLC.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2785-74caee778a1300303e7e4b4c31bdd9b41c6ddf91532f9e830762f2b13a1ebbc53</citedby><cites>FETCH-LOGICAL-c2785-74caee778a1300303e7e4b4c31bdd9b41c6ddf91532f9e830762f2b13a1ebbc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fecj.12425$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fecj.12425$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids></links><search><creatorcontrib>Miyairi, Akitsugu</creatorcontrib><creatorcontrib>Hasegawa, Yuki</creatorcontrib><creatorcontrib>Uchida, Hidekazu</creatorcontrib><title>Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS</title><title>Electronics and communications in Japan</title><description>In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. 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Experimental results showed a decrease in sensor response correlated with inhibitor concentration.</description><subject>acetylcholinesterase</subject><subject>Chemical sensors</subject><subject>drug screening</subject><subject>Electrical measurement</subject><subject>electrochemical imaging sensor</subject><subject>Electrodes</subject><subject>LAAS</subject><subject>micro array</subject><subject>Schottky junction</subject><subject>Substrates</subject><issn>1942-9533</issn><issn>1942-9541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kL1OwzAQxy0EEqUw8AaWmBjS-itxMlZV-VIRAzAwWY5zoa7SuNiJqm48As_Ik2AIYmO6O93vTn_9EDqnZEIJYVMw6wllgqUHaEQLwZIiFfTwr-f8GJ2EsCYkE6ngI_RyDzr0HjbQdtjVWBvo9o1Zuca2EDrwOgDug21fscbdzn2-f1Q2wsG6VjcYGjCdd2YFG2viHOLGebyczR5P0VGtmwBnv3WMnq8WT_ObZPlwfTufLRPDZJ4mUhgNIGWuKSeEEw4SRCkMp2VVFaWgJququqApZ3UBOScyYzUrKdcUytKkfIwuhr9b7976mFmtXe9juKBYXmQkFUTmkbocKONdCB5qtfV2o_1eUaK-zaloTv2Yi-x0YHe2gf3_oFrM74aLL-idcXA</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Miyairi, Akitsugu</creator><creator>Hasegawa, Yuki</creator><creator>Uchida, Hidekazu</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SC</scope><scope>7SP</scope><scope>8FD</scope><scope>JQ2</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>202312</creationdate><title>Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS</title><author>Miyairi, Akitsugu ; Hasegawa, Yuki ; Uchida, Hidekazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2785-74caee778a1300303e7e4b4c31bdd9b41c6ddf91532f9e830762f2b13a1ebbc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>acetylcholinesterase</topic><topic>Chemical sensors</topic><topic>drug screening</topic><topic>Electrical measurement</topic><topic>electrochemical imaging sensor</topic><topic>Electrodes</topic><topic>LAAS</topic><topic>micro array</topic><topic>Schottky junction</topic><topic>Substrates</topic><toplevel>online_resources</toplevel><creatorcontrib>Miyairi, Akitsugu</creatorcontrib><creatorcontrib>Hasegawa, Yuki</creatorcontrib><creatorcontrib>Uchida, Hidekazu</creatorcontrib><collection>CrossRef</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Electronics and communications in Japan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyairi, Akitsugu</au><au>Hasegawa, Yuki</au><au>Uchida, Hidekazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS</atitle><jtitle>Electronics and communications in Japan</jtitle><date>2023-12</date><risdate>2023</risdate><volume>106</volume><issue>4</issue><epage>n/a</epage><issn>1942-9533</issn><eissn>1942-9541</eissn><abstract>In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/ecj.12425</doi><tpages>7</tpages></addata></record> |
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| subjects | acetylcholinesterase Chemical sensors drug screening Electrical measurement electrochemical imaging sensor Electrodes LAAS micro array Schottky junction Substrates |
| title | Measurement of acetylcholinesterase using a two‐dimensional electrochemical sensor LAAS |
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