Detecting fossorial salamanders using eDNA: Development and validation of quantitative and end-point PCR assays for the detection of five species of Ambystoma

In the past decade, quantification of environmental DNA (eDNA) has become firmly established as an effective method for detecting the presence of organisms of research and conservation interest. Salamanders of the family Ambystomatidae are large, fossorial species; adults are rarely encountered abov...

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