Microbial degradation mechanism of historical silk revealed by proteomics and metabolomics
Archaeological silk undergoes destructive and irreversible changes during the natural process of decay. However, in-depth studies on the influence of this biological factor are still lacking. Here, a combination of proteomics and metabolomics is proposed for the first time to explore the interaction...
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| Veröffentlicht in: | Analytical methods 2023-10, Vol.15 (4), p.538-5389 |
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| creator | Pan, Lindan Ding, Chuanmiao Deng, Yefeng Chen, Hao Yang, Hailiang Wang, Biyang Zhou, Yang Wang, Bing |
| description | Archaeological silk undergoes destructive and irreversible changes during the natural process of decay. However, in-depth studies on the influence of this biological factor are still lacking. Here, a combination of proteomics and metabolomics is proposed for the first time to explore the interaction between bacteria and historical silk during biodegradation, which provides information on changes at the molecular level of proteins and bacterial metabolites. Morphological observation revealed biofilms produced by
Stenotrophomonas maltophilia
and
Pseudomonas alcaligenes
when cultured in the stationary phase and confirmed severe deterioration of silk. Proteomics showed that
S. maltophilia
had an unbiased effect on silk fibroin, indicating its ability to disrupt both heavy and light chains, as well as other proteins, while
P. alcaligenes
showed an affinity for more disordered proteins. Analysis of bacterial metabolites showed that overall activity reduction and significant accumulation of fatty acid and phenol metabolites occurred after silk addition, suggesting that the presence of silk may inhibit the activity of an individual strain. This study provides a new insight into the microbial degradation mechanism of archaeological silk.
Archaeological silk undergoes destructive and irreversible changes during the natural process of decay. |
| doi_str_mv | 10.1039/d3ay01033c |
| format | Article |
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Stenotrophomonas maltophilia
and
Pseudomonas alcaligenes
when cultured in the stationary phase and confirmed severe deterioration of silk. Proteomics showed that
S. maltophilia
had an unbiased effect on silk fibroin, indicating its ability to disrupt both heavy and light chains, as well as other proteins, while
P. alcaligenes
showed an affinity for more disordered proteins. Analysis of bacterial metabolites showed that overall activity reduction and significant accumulation of fatty acid and phenol metabolites occurred after silk addition, suggesting that the presence of silk may inhibit the activity of an individual strain. This study provides a new insight into the microbial degradation mechanism of archaeological silk.
Archaeological silk undergoes destructive and irreversible changes during the natural process of decay.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/d3ay01033c</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Alcaligenes ; Archaeology ; Bacteria ; Biodegradation ; Biofilms ; Decay ; Degradation ; Fatty acids ; Light chains ; Metabolites ; Metabolomics ; Microbial degradation ; Microorganisms ; Phenols ; Proteins ; Proteomics ; Silk ; Silk fibroin ; Stationary phase</subject><ispartof>Analytical methods, 2023-10, Vol.15 (4), p.538-5389</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-1fb7dfa9ee7d7ceace647e99f8c2d8f6062154ec1e9871c51ade0fb958082933</citedby><cites>FETCH-LOGICAL-c314t-1fb7dfa9ee7d7ceace647e99f8c2d8f6062154ec1e9871c51ade0fb958082933</cites><orcidid>0000-0002-3499-7828</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Pan, Lindan</creatorcontrib><creatorcontrib>Ding, Chuanmiao</creatorcontrib><creatorcontrib>Deng, Yefeng</creatorcontrib><creatorcontrib>Chen, Hao</creatorcontrib><creatorcontrib>Yang, Hailiang</creatorcontrib><creatorcontrib>Wang, Biyang</creatorcontrib><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Wang, Bing</creatorcontrib><title>Microbial degradation mechanism of historical silk revealed by proteomics and metabolomics</title><title>Analytical methods</title><description>Archaeological silk undergoes destructive and irreversible changes during the natural process of decay. However, in-depth studies on the influence of this biological factor are still lacking. Here, a combination of proteomics and metabolomics is proposed for the first time to explore the interaction between bacteria and historical silk during biodegradation, which provides information on changes at the molecular level of proteins and bacterial metabolites. Morphological observation revealed biofilms produced by
Stenotrophomonas maltophilia
and
Pseudomonas alcaligenes
when cultured in the stationary phase and confirmed severe deterioration of silk. Proteomics showed that
S. maltophilia
had an unbiased effect on silk fibroin, indicating its ability to disrupt both heavy and light chains, as well as other proteins, while
P. alcaligenes
showed an affinity for more disordered proteins. Analysis of bacterial metabolites showed that overall activity reduction and significant accumulation of fatty acid and phenol metabolites occurred after silk addition, suggesting that the presence of silk may inhibit the activity of an individual strain. This study provides a new insight into the microbial degradation mechanism of archaeological silk.
Archaeological silk undergoes destructive and irreversible changes during the natural process of decay.</description><subject>Alcaligenes</subject><subject>Archaeology</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Biofilms</subject><subject>Decay</subject><subject>Degradation</subject><subject>Fatty acids</subject><subject>Light chains</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Microbial degradation</subject><subject>Microorganisms</subject><subject>Phenols</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Silk</subject><subject>Silk fibroin</subject><subject>Stationary phase</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpd0U1LAzEQBuBFFKzVi3ch4EWE1aTZzcex1E-oeOlFL0s2mdjU3U1NtkL_vWkrFTxlCM8MwztZdk7wDcFU3hqq1jhVVB9kA8JLmUvG5eG-Zvg4O4lxgTGTlJFB9v7idPC1Uw0y8BGUUb3zHWpBz1XnYou8RXMXex-cTia65hMF-AbVgEH1Gi2D78G3TkekOpP6elX7Zvtxmh1Z1UQ4-32H2ezhfjZ5yqevj8-T8TTXlBR9TmzNjVUSgBuuQWlgBQcprdAjIyzDbETKAjQBKTjRJVEGsK1lKbAYSUqH2dVubFrlawWxr1oXNTSN6sCvYjUSvBC0KGSR6OU_uvCr0KXlNkowUeKtut6pFEyMAWy1DK5VYV0RXG1Sru7o-G2b8iThix0OUe_d3xXoD4tQep0</recordid><startdate>20231019</startdate><enddate>20231019</enddate><creator>Pan, Lindan</creator><creator>Ding, Chuanmiao</creator><creator>Deng, Yefeng</creator><creator>Chen, Hao</creator><creator>Yang, Hailiang</creator><creator>Wang, Biyang</creator><creator>Zhou, Yang</creator><creator>Wang, Bing</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3499-7828</orcidid></search><sort><creationdate>20231019</creationdate><title>Microbial degradation mechanism of historical silk revealed by proteomics and metabolomics</title><author>Pan, Lindan ; 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However, in-depth studies on the influence of this biological factor are still lacking. Here, a combination of proteomics and metabolomics is proposed for the first time to explore the interaction between bacteria and historical silk during biodegradation, which provides information on changes at the molecular level of proteins and bacterial metabolites. Morphological observation revealed biofilms produced by
Stenotrophomonas maltophilia
and
Pseudomonas alcaligenes
when cultured in the stationary phase and confirmed severe deterioration of silk. Proteomics showed that
S. maltophilia
had an unbiased effect on silk fibroin, indicating its ability to disrupt both heavy and light chains, as well as other proteins, while
P. alcaligenes
showed an affinity for more disordered proteins. Analysis of bacterial metabolites showed that overall activity reduction and significant accumulation of fatty acid and phenol metabolites occurred after silk addition, suggesting that the presence of silk may inhibit the activity of an individual strain. This study provides a new insight into the microbial degradation mechanism of archaeological silk.
Archaeological silk undergoes destructive and irreversible changes during the natural process of decay.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d3ay01033c</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3499-7828</orcidid></addata></record> |
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| language | eng |
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| source | Royal Society Of Chemistry Journals |
| subjects | Alcaligenes Archaeology Bacteria Biodegradation Biofilms Decay Degradation Fatty acids Light chains Metabolites Metabolomics Microbial degradation Microorganisms Phenols Proteins Proteomics Silk Silk fibroin Stationary phase |
| title | Microbial degradation mechanism of historical silk revealed by proteomics and metabolomics |
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