Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa
This study determined the prevalence, risk factors, and molecular characteristics of Listeria species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emala...
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| Veröffentlicht in: | Journal of food safety 2023-10, Vol.43 (5) |
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| creator | Moabelo, Khomotso C. Gcebe, Nomakorinte Gana, James Ngoshe, Yusuf B. Adesiyun, Abiodun A. |
| description | This study determined the prevalence, risk factors, and molecular characteristics of
Listeria
species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emalahleni, and Middelburg) within the province. Standard bacteriological and polymerase chain reaction (PCR) assays were used in the study. The overall prevalence of
L. monocytogenes
and other
Listeria
spp. in the samples was 8.3% (33/400) and 30% (120/400) (
p
|
| doi_str_mv | 10.1111/jfs.13055 |
| format | Article |
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Listeria
species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emalahleni, and Middelburg) within the province. Standard bacteriological and polymerase chain reaction (PCR) assays were used in the study. The overall prevalence of
L. monocytogenes
and other
Listeria
spp. in the samples was 8.3% (33/400) and 30% (120/400) (
p
< .05), respectively. For the five variables investigated, statistically significant effects were evident only for the region (
p
< .001) and type of product (
p
< .0001) for
L. monocytogenes
, the type of outlet (
p
= .011) and the type of product (
p
< .0001) for
Listeria
spp. Of the 20 types of beef and beef products tested, 15 (75%) and 17 (85%) were contaminated by
L. monocytogenes
and
Listeria
spp., respectively (
p
= .429). Among the four categories of products tested, the prevalence of
L. monocytogenes
was 7.3% (8/109), 10.6% (11/104), 7.5% (8/106), and 7.4% (6/81) for raw beef, ready‐to‐eat (RTE) products, milled beef, and offal & organs, respectively (
p
> .799). Among the 33
L. monocytogenes
isolates, PCR genoserogroup IIa (42.4%, 1/2a‐3a) was most frequently detected. All (100%) of the isolates carried one or more of the eight virulence‐associated genes assessed, with genes
inlC
and
inlJ
detected in all the isolates
.
The overall prevalence of
L. monocytogenes
(8.3%) and the high frequency of virulent serogroups of
L. monocytogenes
commonly associated with human listeriosis pose a food safety risk to consumers of beef and beef‐based products contaminated by
L. monocytogenes
.</description><identifier>ISSN: 0149-6085</identifier><identifier>EISSN: 1745-4565</identifier><identifier>DOI: 10.1111/jfs.13055</identifier><language>eng</language><publisher>Westport: Blackwell Publishers Inc</publisher><subject>Beef ; Contamination ; Food contamination ; Food safety ; Genes ; Listeria ; Listeriosis ; Polymerase chain reaction ; Risk factors ; Statistical analysis ; Virulence</subject><ispartof>Journal of food safety, 2023-10, Vol.43 (5)</ispartof><rights>2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-a825119c6a640d27eee0a777e4b3c59a1ac155792669501fc87a712fdb15c8553</citedby><cites>FETCH-LOGICAL-c292t-a825119c6a640d27eee0a777e4b3c59a1ac155792669501fc87a712fdb15c8553</cites><orcidid>0000-0002-7559-6617 ; 0000-0001-9415-5297 ; 0000-0001-9470-9421</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Moabelo, Khomotso C.</creatorcontrib><creatorcontrib>Gcebe, Nomakorinte</creatorcontrib><creatorcontrib>Gana, James</creatorcontrib><creatorcontrib>Ngoshe, Yusuf B.</creatorcontrib><creatorcontrib>Adesiyun, Abiodun A.</creatorcontrib><title>Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa</title><title>Journal of food safety</title><description>This study determined the prevalence, risk factors, and molecular characteristics of
Listeria
species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emalahleni, and Middelburg) within the province. Standard bacteriological and polymerase chain reaction (PCR) assays were used in the study. The overall prevalence of
L. monocytogenes
and other
Listeria
spp. in the samples was 8.3% (33/400) and 30% (120/400) (
p
< .05), respectively. For the five variables investigated, statistically significant effects were evident only for the region (
p
< .001) and type of product (
p
< .0001) for
L. monocytogenes
, the type of outlet (
p
= .011) and the type of product (
p
< .0001) for
Listeria
spp. Of the 20 types of beef and beef products tested, 15 (75%) and 17 (85%) were contaminated by
L. monocytogenes
and
Listeria
spp., respectively (
p
= .429). Among the four categories of products tested, the prevalence of
L. monocytogenes
was 7.3% (8/109), 10.6% (11/104), 7.5% (8/106), and 7.4% (6/81) for raw beef, ready‐to‐eat (RTE) products, milled beef, and offal & organs, respectively (
p
> .799). Among the 33
L. monocytogenes
isolates, PCR genoserogroup IIa (42.4%, 1/2a‐3a) was most frequently detected. All (100%) of the isolates carried one or more of the eight virulence‐associated genes assessed, with genes
inlC
and
inlJ
detected in all the isolates
.
The overall prevalence of
L. monocytogenes
(8.3%) and the high frequency of virulent serogroups of
L. monocytogenes
commonly associated with human listeriosis pose a food safety risk to consumers of beef and beef‐based products contaminated by
L. monocytogenes
.</description><subject>Beef</subject><subject>Contamination</subject><subject>Food contamination</subject><subject>Food safety</subject><subject>Genes</subject><subject>Listeria</subject><subject>Listeriosis</subject><subject>Polymerase chain reaction</subject><subject>Risk factors</subject><subject>Statistical analysis</subject><subject>Virulence</subject><issn>0149-6085</issn><issn>1745-4565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNo9kM9KxDAQh4MouK4efIOAJ8HWJG2a9rgs_oOKB_Vcpmmy26VNapIe1hfwte1uxbnMwHz8hvkQuqYkplPd77SPaUI4P0ELKlIepTzjp2hBaFpEGcn5ObrwfkdIkjGWLNDP2poAfWsgtNZgq3GtlMZgmnkYnG1GGTyu97hsfVCuBeyHIT4ive2UHDtwWG7BgTysv_-TyngCjJX7YDfKKI9bg1-HsYcOzAbu8LsdwxavtGslXKIzDZ1XV399iT4fHz7Wz1H59vSyXpWRZAULEeSMU1rIDLKUNEwopQgIIVRaJ5IXQEFSzkXBsqzghGqZCxCU6aamXOacJ0t0M-dOj32NyodqZ0dnppMVy0U6iUt5PlG3MyWd9d4pXQ2u7cHtK0qqg-dq8lwdPSe_wspxOQ</recordid><startdate>202310</startdate><enddate>202310</enddate><creator>Moabelo, Khomotso C.</creator><creator>Gcebe, Nomakorinte</creator><creator>Gana, James</creator><creator>Ngoshe, Yusuf B.</creator><creator>Adesiyun, Abiodun A.</creator><general>Blackwell Publishers Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T2</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-7559-6617</orcidid><orcidid>https://orcid.org/0000-0001-9415-5297</orcidid><orcidid>https://orcid.org/0000-0001-9470-9421</orcidid></search><sort><creationdate>202310</creationdate><title>Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa</title><author>Moabelo, Khomotso C. ; Gcebe, Nomakorinte ; Gana, James ; Ngoshe, Yusuf B. ; Adesiyun, Abiodun A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-a825119c6a640d27eee0a777e4b3c59a1ac155792669501fc87a712fdb15c8553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Beef</topic><topic>Contamination</topic><topic>Food contamination</topic><topic>Food safety</topic><topic>Genes</topic><topic>Listeria</topic><topic>Listeriosis</topic><topic>Polymerase chain reaction</topic><topic>Risk factors</topic><topic>Statistical analysis</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moabelo, Khomotso C.</creatorcontrib><creatorcontrib>Gcebe, Nomakorinte</creatorcontrib><creatorcontrib>Gana, James</creatorcontrib><creatorcontrib>Ngoshe, Yusuf B.</creatorcontrib><creatorcontrib>Adesiyun, Abiodun A.</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of food safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moabelo, Khomotso C.</au><au>Gcebe, Nomakorinte</au><au>Gana, James</au><au>Ngoshe, Yusuf B.</au><au>Adesiyun, Abiodun A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa</atitle><jtitle>Journal of food safety</jtitle><date>2023-10</date><risdate>2023</risdate><volume>43</volume><issue>5</issue><issn>0149-6085</issn><eissn>1745-4565</eissn><abstract>This study determined the prevalence, risk factors, and molecular characteristics of
Listeria
species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emalahleni, and Middelburg) within the province. Standard bacteriological and polymerase chain reaction (PCR) assays were used in the study. The overall prevalence of
L. monocytogenes
and other
Listeria
spp. in the samples was 8.3% (33/400) and 30% (120/400) (
p
< .05), respectively. For the five variables investigated, statistically significant effects were evident only for the region (
p
< .001) and type of product (
p
< .0001) for
L. monocytogenes
, the type of outlet (
p
= .011) and the type of product (
p
< .0001) for
Listeria
spp. Of the 20 types of beef and beef products tested, 15 (75%) and 17 (85%) were contaminated by
L. monocytogenes
and
Listeria
spp., respectively (
p
= .429). Among the four categories of products tested, the prevalence of
L. monocytogenes
was 7.3% (8/109), 10.6% (11/104), 7.5% (8/106), and 7.4% (6/81) for raw beef, ready‐to‐eat (RTE) products, milled beef, and offal & organs, respectively (
p
> .799). Among the 33
L. monocytogenes
isolates, PCR genoserogroup IIa (42.4%, 1/2a‐3a) was most frequently detected. All (100%) of the isolates carried one or more of the eight virulence‐associated genes assessed, with genes
inlC
and
inlJ
detected in all the isolates
.
The overall prevalence of
L. monocytogenes
(8.3%) and the high frequency of virulent serogroups of
L. monocytogenes
commonly associated with human listeriosis pose a food safety risk to consumers of beef and beef‐based products contaminated by
L. monocytogenes
.</abstract><cop>Westport</cop><pub>Blackwell Publishers Inc</pub><doi>10.1111/jfs.13055</doi><orcidid>https://orcid.org/0000-0002-7559-6617</orcidid><orcidid>https://orcid.org/0000-0001-9415-5297</orcidid><orcidid>https://orcid.org/0000-0001-9470-9421</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Beef Contamination Food contamination Food safety Genes Listeria Listeriosis Polymerase chain reaction Risk factors Statistical analysis Virulence |
| title | Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa |
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