Exploration and Inhibition Test of Penicillium sp. In Vitro by Trichoderma
Penicillium is an entomopathogenic fungus which can be used as a bio-bactericidal. Its existence in nature can be found together with other microbes including Trichoderma, a biocontrol agent that has the ability to damage the cell walls of other fungi due to the activity of the extracellular enzyme...
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| Veröffentlicht in: | IOP conference series. Earth and environmental science 2023-09, Vol.1242 (1), p.12012 |
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| creator | Yuliantoro, I D Prihatiningrum, A E Sutarman |
| description | Penicillium
is an entomopathogenic fungus which can be used as a bio-bactericidal. Its existence in nature can be found together with other microbes including Trichoderma, a biocontrol agent that has the ability to damage the cell walls of other fungi due to the activity of the extracellular enzyme chitinase it produces. This study aims to obtain potential isolates of
Penicillium
biocontrol agents from vegetable growing fields and to determine their response to inhibition by
Trichoderma esperellum
in vitro. On PDA-chloramphenicol media, a suspension of 10
4
dilution was inoculated containing sample soil from vegetable crops to be isolated and purified and identified as to its species. The entomopathogenic isolates obtained were grown together with
T. esperellum
in dual culture and also grown in monoculture as a comparison. The isolation results obtained
Penicillium
sp. Pc-02. The in vitro test results showed that
Trichoderma
isolates could inhibit
Penicillium
sp. by 35.5 ± 1.9% and supported the growth of this entomopagen fungus by 15.7% at 24 and 72 hours after inoculation, respectively. |
| doi_str_mv | 10.1088/1755-1315/1242/1/012012 |
| format | Article |
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is an entomopathogenic fungus which can be used as a bio-bactericidal. Its existence in nature can be found together with other microbes including Trichoderma, a biocontrol agent that has the ability to damage the cell walls of other fungi due to the activity of the extracellular enzyme chitinase it produces. This study aims to obtain potential isolates of
Penicillium
biocontrol agents from vegetable growing fields and to determine their response to inhibition by
Trichoderma esperellum
in vitro. On PDA-chloramphenicol media, a suspension of 10
4
dilution was inoculated containing sample soil from vegetable crops to be isolated and purified and identified as to its species. The entomopathogenic isolates obtained were grown together with
T. esperellum
in dual culture and also grown in monoculture as a comparison. The isolation results obtained
Penicillium
sp. Pc-02. The in vitro test results showed that
Trichoderma
isolates could inhibit
Penicillium
sp. by 35.5 ± 1.9% and supported the growth of this entomopagen fungus by 15.7% at 24 and 72 hours after inoculation, respectively.</description><identifier>ISSN: 1755-1307</identifier><identifier>EISSN: 1755-1315</identifier><identifier>DOI: 10.1088/1755-1315/1242/1/012012</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Biological control ; Cell culture ; Cell walls ; Chitinase ; Chloramphenicol ; Chloromycetin ; Dilution ; Entomopathogenic fungi ; Fungi ; In vitro methods and tests ; Inhibition Test ; Inoculation ; Monoculture ; ofPenicillium sp ; Penicillium ; Trichoderma ; Vegetables</subject><ispartof>IOP conference series. Earth and environmental science, 2023-09, Vol.1242 (1), p.12012</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>Published under licence by IOP Publishing Ltd. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3272-db7e48c6f833a202c015f10cb0e3efb2721c5bbce0c73ffb0dcff9659d43878b3</citedby><cites>FETCH-LOGICAL-c3272-db7e48c6f833a202c015f10cb0e3efb2721c5bbce0c73ffb0dcff9659d43878b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1755-1315/1242/1/012012/pdf$$EPDF$$P50$$Giop$$Hfree_for_read</linktopdf><link.rule.ids>314,780,784,27924,27925,38868,38890,53840,53867</link.rule.ids></links><search><creatorcontrib>Yuliantoro, I D</creatorcontrib><creatorcontrib>Prihatiningrum, A E</creatorcontrib><creatorcontrib>Sutarman</creatorcontrib><title>Exploration and Inhibition Test of Penicillium sp. In Vitro by Trichoderma</title><title>IOP conference series. Earth and environmental science</title><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><description>Penicillium
is an entomopathogenic fungus which can be used as a bio-bactericidal. Its existence in nature can be found together with other microbes including Trichoderma, a biocontrol agent that has the ability to damage the cell walls of other fungi due to the activity of the extracellular enzyme chitinase it produces. This study aims to obtain potential isolates of
Penicillium
biocontrol agents from vegetable growing fields and to determine their response to inhibition by
Trichoderma esperellum
in vitro. On PDA-chloramphenicol media, a suspension of 10
4
dilution was inoculated containing sample soil from vegetable crops to be isolated and purified and identified as to its species. The entomopathogenic isolates obtained were grown together with
T. esperellum
in dual culture and also grown in monoculture as a comparison. The isolation results obtained
Penicillium
sp. Pc-02. The in vitro test results showed that
Trichoderma
isolates could inhibit
Penicillium
sp. by 35.5 ± 1.9% and supported the growth of this entomopagen fungus by 15.7% at 24 and 72 hours after inoculation, respectively.</description><subject>Biological control</subject><subject>Cell culture</subject><subject>Cell walls</subject><subject>Chitinase</subject><subject>Chloramphenicol</subject><subject>Chloromycetin</subject><subject>Dilution</subject><subject>Entomopathogenic fungi</subject><subject>Fungi</subject><subject>In vitro methods and tests</subject><subject>Inhibition Test</subject><subject>Inoculation</subject><subject>Monoculture</subject><subject>ofPenicillium sp</subject><subject>Penicillium</subject><subject>Trichoderma</subject><subject>Vegetables</subject><issn>1755-1307</issn><issn>1755-1315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>O3W</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkF1LwzAUhoMoOKe_wYBXXnTNR9uklzKqTgYKTm9DkyYso2tqsoH796ZWFEEQAskhz3nP4QHgEqMZRpynmOV5ginOU0wykuIUYRLPEZh8_xx_vxE7BWchbBAqWEbLCXio3vvW-XpnXQfrroGLbm2l_SxXOuygM_BJd1bZtrX7LQz9LCLw1e68g_IAV96qtWu039bn4MTUbdAXX_cUvNxWq_l9sny8W8xvlomihJGkkUxnXBWGU1oTRBTCucFISaSpNjIiWOVSKo0Uo8ZI1ChjyiIvm4xyxiWdgqsxt_fubR93FBu3910cKQgvyoIjxEmk2Egp70Lw2oje223tDwIjMYgTgxIx6BGDOIHFKC52Xo-d1vU_0VX1_JsTfWMiS_9g_5vwAVFgfFA</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Yuliantoro, I D</creator><creator>Prihatiningrum, A E</creator><creator>Sutarman</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope></search><sort><creationdate>20230901</creationdate><title>Exploration and Inhibition Test of Penicillium sp. 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Penicillium
biocontrol agents from vegetable growing fields and to determine their response to inhibition by
Trichoderma esperellum
in vitro. On PDA-chloramphenicol media, a suspension of 10
4
dilution was inoculated containing sample soil from vegetable crops to be isolated and purified and identified as to its species. The entomopathogenic isolates obtained were grown together with
T. esperellum
in dual culture and also grown in monoculture as a comparison. The isolation results obtained
Penicillium
sp. Pc-02. The in vitro test results showed that
Trichoderma
isolates could inhibit
Penicillium
sp. by 35.5 ± 1.9% and supported the growth of this entomopagen fungus by 15.7% at 24 and 72 hours after inoculation, respectively.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1755-1315/1242/1/012012</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Institute of Physics; Institute of Physics Open Access Journal Titles; EZB Electronic Journals Library |
| subjects | Biological control Cell culture Cell walls Chitinase Chloramphenicol Chloromycetin Dilution Entomopathogenic fungi Fungi In vitro methods and tests Inhibition Test Inoculation Monoculture ofPenicillium sp Penicillium Trichoderma Vegetables |
| title | Exploration and Inhibition Test of Penicillium sp. In Vitro by Trichoderma |
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