A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas
Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition of hTPC activity by naringenin, one of the main flavonoids present...
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| Veröffentlicht in: | Biomolecular Concepts 2023-09, Vol.14 (1), p.1143-55 |
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| creator | Minicozzi, Velia Qi, Tianwen Gradogna, Antonella Pozzolini, Marina Milenkovic, Stefan Filippini, Antonio Ceccarelli, Matteo Carpaneto, Armando |
| description | Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition of hTPC activity by naringenin, one of the main flavonoids present in the human diet, has the potential to be beneficial in severe human diseases such as solid tumor development, melanoma, and viral infections. We attempted to identify the molecular basis of the interaction between hTPC2 and naringenin, using ensemble docking on molecular dynamics (MD) trajectories, but the specific binding site remains elusive, posing a challenge that could potentially be addressed in the future by increased computational power in MD and the combined use of microscopy techniques such as cryo-EM. |
| doi_str_mv | 10.1515/bmc-2022-0036 |
| format | Article |
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| source | De Gruyter Open Access Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Flavonoids Lysosomes Melanoma Naringenin patch-clamp plant vacuole Solid tumors tpc channels Vacuoles |
| title | A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas |
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