A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles
We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H 3 PO 4 , KH 2 PO 4 , and K 2 H...
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| creator | Fernández-Penas, Raquel Verdugo-Escamilla, Cristóbal Triunfo, Carla Gärtner, Stefanie D'Urso, Annarita Oltolina, Francesca Follenzi, Antonia Maoloni, Gabriele Cölfen, Helmut Falini, Giuseppe Gómez-Morales, Jaime |
| description | We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species
Crassostrea gigas
(Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H
3
PO
4
, KH
2
PO
4
, and K
2
HPO
4
), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K
2
HPO
4
instead of KH
2
PO
4
at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place
via
a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.
A straightforward, one-pot, low-temperature hydrothermal method has been set up to transform calcium carbonate particles from oyster shell waste into hydroxyapatite micro/nanoparticles with osteoinductive properties. |
| doi_str_mv | 10.1039/d3tb00856h |
| format | Article |
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Crassostrea gigas
(Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H
3
PO
4
, KH
2
PO
4
, and K
2
HPO
4
), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K
2
HPO
4
instead of KH
2
PO
4
at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place
via
a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.
A straightforward, one-pot, low-temperature hydrothermal method has been set up to transform calcium carbonate particles from oyster shell waste into hydroxyapatite micro/nanoparticles with osteoinductive properties.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/d3tb00856h</identifier><identifier>PMID: 37476854</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Calcite ; Calcium carbonate ; Differentiation (biology) ; Digestion ; Dissolution ; Hydroxyapatite ; Low temperature ; Mesenchymal stem cells ; Nanoparticles ; Osteogenesis ; Osteosarcoma ; Osteosarcoma cells ; Oysters ; Potassium phosphate ; Potassium phosphates ; Reagents ; Stem cells ; Tissue engineering ; Transformations</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2023-09, Vol.11 (32), p.7766-7777</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-b468dbef6fe853c0533f114a41bf2723f806410e22031d542412f04af0cc08873</citedby><cites>FETCH-LOGICAL-c373t-b468dbef6fe853c0533f114a41bf2723f806410e22031d542412f04af0cc08873</cites><orcidid>0000-0001-9780-300X ; 0009-0009-1016-8468 ; 0000-0001-5229-0757 ; 0000-0002-0613-6166 ; 0000-0002-9395-7797 ; 0000-0002-2367-3721 ; 0000-0002-8290-9677 ; 0000-0003-2345-8359 ; 0000-0002-1148-0308</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37476854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández-Penas, Raquel</creatorcontrib><creatorcontrib>Verdugo-Escamilla, Cristóbal</creatorcontrib><creatorcontrib>Triunfo, Carla</creatorcontrib><creatorcontrib>Gärtner, Stefanie</creatorcontrib><creatorcontrib>D'Urso, Annarita</creatorcontrib><creatorcontrib>Oltolina, Francesca</creatorcontrib><creatorcontrib>Follenzi, Antonia</creatorcontrib><creatorcontrib>Maoloni, Gabriele</creatorcontrib><creatorcontrib>Cölfen, Helmut</creatorcontrib><creatorcontrib>Falini, Giuseppe</creatorcontrib><creatorcontrib>Gómez-Morales, Jaime</creatorcontrib><title>A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species
Crassostrea gigas
(Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H
3
PO
4
, KH
2
PO
4
, and K
2
HPO
4
), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K
2
HPO
4
instead of KH
2
PO
4
at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place
via
a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.
A straightforward, one-pot, low-temperature hydrothermal method has been set up to transform calcium carbonate particles from oyster shell waste into hydroxyapatite micro/nanoparticles with osteoinductive properties.</description><subject>Calcite</subject><subject>Calcium carbonate</subject><subject>Differentiation (biology)</subject><subject>Digestion</subject><subject>Dissolution</subject><subject>Hydroxyapatite</subject><subject>Low temperature</subject><subject>Mesenchymal stem cells</subject><subject>Nanoparticles</subject><subject>Osteogenesis</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma cells</subject><subject>Oysters</subject><subject>Potassium phosphate</subject><subject>Potassium phosphates</subject><subject>Reagents</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transformations</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpd0U1LwzAYB_AgihPdxbsS8CJCNW9t41Hny4SBlwneSpomLKNNapKqO_nVjU4nmEtCnl8e8vAH4BCjc4zo5UVDY40Qz4vFFtgjKEdZmWO-vTmj5xEYh7BEaXFccMp2wYiWrCx4zvbAxxUMQ4jCWFG3Cjqrst5F2Km4cA2MDkYvbNDOdzAoERaqbeGbCFFBKVpphi7tvnZWpJukXao4Y5tBRvOq4GLVePe-Er2IJoHOSO8yK6zrhY9GtiocgB0t2qDGP_s-eLq7nU-m2ezx_mFyNcskLWnMalbwpla60IrnVKKcUo0xEwzXmpSEao4KhpEiBFHc5IwwTDRiQiMpEecl3Qen6769dy-DCrHqTJBpGmGVG0JFeHpOLktCEj35R5du8Db9LqkCU8ZzzpM6W6s0Ughe6ar3phN-VWFUfSVT3dD59Xcy04SPf1oOdaeaDf3NIYGjNfBBbqp_0dJPTcyUWQ</recordid><startdate>20230906</startdate><enddate>20230906</enddate><creator>Fernández-Penas, Raquel</creator><creator>Verdugo-Escamilla, Cristóbal</creator><creator>Triunfo, Carla</creator><creator>Gärtner, Stefanie</creator><creator>D'Urso, Annarita</creator><creator>Oltolina, Francesca</creator><creator>Follenzi, Antonia</creator><creator>Maoloni, Gabriele</creator><creator>Cölfen, Helmut</creator><creator>Falini, Giuseppe</creator><creator>Gómez-Morales, Jaime</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9780-300X</orcidid><orcidid>https://orcid.org/0009-0009-1016-8468</orcidid><orcidid>https://orcid.org/0000-0001-5229-0757</orcidid><orcidid>https://orcid.org/0000-0002-0613-6166</orcidid><orcidid>https://orcid.org/0000-0002-9395-7797</orcidid><orcidid>https://orcid.org/0000-0002-2367-3721</orcidid><orcidid>https://orcid.org/0000-0002-8290-9677</orcidid><orcidid>https://orcid.org/0000-0003-2345-8359</orcidid><orcidid>https://orcid.org/0000-0002-1148-0308</orcidid></search><sort><creationdate>20230906</creationdate><title>A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles</title><author>Fernández-Penas, Raquel ; Verdugo-Escamilla, Cristóbal ; Triunfo, Carla ; Gärtner, Stefanie ; D'Urso, Annarita ; Oltolina, Francesca ; Follenzi, Antonia ; Maoloni, Gabriele ; Cölfen, Helmut ; Falini, Giuseppe ; Gómez-Morales, Jaime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-b468dbef6fe853c0533f114a41bf2723f806410e22031d542412f04af0cc08873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Calcite</topic><topic>Calcium carbonate</topic><topic>Differentiation (biology)</topic><topic>Digestion</topic><topic>Dissolution</topic><topic>Hydroxyapatite</topic><topic>Low temperature</topic><topic>Mesenchymal stem cells</topic><topic>Nanoparticles</topic><topic>Osteogenesis</topic><topic>Osteosarcoma</topic><topic>Osteosarcoma cells</topic><topic>Oysters</topic><topic>Potassium phosphate</topic><topic>Potassium phosphates</topic><topic>Reagents</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transformations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández-Penas, Raquel</creatorcontrib><creatorcontrib>Verdugo-Escamilla, Cristóbal</creatorcontrib><creatorcontrib>Triunfo, Carla</creatorcontrib><creatorcontrib>Gärtner, Stefanie</creatorcontrib><creatorcontrib>D'Urso, Annarita</creatorcontrib><creatorcontrib>Oltolina, Francesca</creatorcontrib><creatorcontrib>Follenzi, Antonia</creatorcontrib><creatorcontrib>Maoloni, Gabriele</creatorcontrib><creatorcontrib>Cölfen, Helmut</creatorcontrib><creatorcontrib>Falini, Giuseppe</creatorcontrib><creatorcontrib>Gómez-Morales, Jaime</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández-Penas, Raquel</au><au>Verdugo-Escamilla, Cristóbal</au><au>Triunfo, Carla</au><au>Gärtner, Stefanie</au><au>D'Urso, Annarita</au><au>Oltolina, Francesca</au><au>Follenzi, Antonia</au><au>Maoloni, Gabriele</au><au>Cölfen, Helmut</au><au>Falini, Giuseppe</au><au>Gómez-Morales, Jaime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2023-09-06</date><risdate>2023</risdate><volume>11</volume><issue>32</issue><spage>7766</spage><epage>7777</epage><pages>7766-7777</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species
Crassostrea gigas
(Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H
3
PO
4
, KH
2
PO
4
, and K
2
HPO
4
), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K
2
HPO
4
instead of KH
2
PO
4
at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place
via
a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.
A straightforward, one-pot, low-temperature hydrothermal method has been set up to transform calcium carbonate particles from oyster shell waste into hydroxyapatite micro/nanoparticles with osteoinductive properties.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>37476854</pmid><doi>10.1039/d3tb00856h</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9780-300X</orcidid><orcidid>https://orcid.org/0009-0009-1016-8468</orcidid><orcidid>https://orcid.org/0000-0001-5229-0757</orcidid><orcidid>https://orcid.org/0000-0002-0613-6166</orcidid><orcidid>https://orcid.org/0000-0002-9395-7797</orcidid><orcidid>https://orcid.org/0000-0002-2367-3721</orcidid><orcidid>https://orcid.org/0000-0002-8290-9677</orcidid><orcidid>https://orcid.org/0000-0003-2345-8359</orcidid><orcidid>https://orcid.org/0000-0002-1148-0308</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2050-750X |
| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2023-09, Vol.11 (32), p.7766-7777 |
| issn | 2050-750X 2050-7518 |
| language | eng |
| recordid | cdi_proquest_journals_2861348588 |
| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Calcite Calcium carbonate Differentiation (biology) Digestion Dissolution Hydroxyapatite Low temperature Mesenchymal stem cells Nanoparticles Osteogenesis Osteosarcoma Osteosarcoma cells Oysters Potassium phosphate Potassium phosphates Reagents Stem cells Tissue engineering Transformations |
| title | A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles |
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