An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum
Plant roots can sense the nitrogen level in the environment and change the expression of related genes to maintain normal life activities. However, their regulatory mechanism in Chrysanthemum has not been fully clarified. In this study, an R2R3-MYB transcription factor CmMYB42 was isolated from Chry...
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| Veröffentlicht in: | Journal of plant growth regulation 2023-09, Vol.42 (9), p.5600-5614 |
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| creator | Ma, Rui Yang, Sen Liu, Yanhong Sun, Xia Wang, Wenli Zheng, Chengshu |
| description | Plant roots can sense the nitrogen level in the environment and change the expression of related genes to maintain normal life activities. However, their regulatory mechanism in
Chrysanthemum
has not been fully clarified. In this study, an R2R3-MYB transcription factor
CmMYB42
was isolated from
Chrysanthemum
‘Jinba.’ Tissue expression analysis showed that the gene was mainly expressed in the root and could rapidly up-regulate the expression of
CmMYB42
under low-nitrogen conditions. Subcellular localization analysis showed that
CmMYB42
was located in the nucleus. Under low-nitrogen stress, overexpressing
CmMYB42 Chrysanthemum
’s total root length, lateral root number, plant height, and biomass accumulation were significantly higher than wild type and slowed down the damage to photosynthetic system and improved NO
3
−
content and nitrogen metabolism assimilation enzyme activity. In addition, we also found that
CmMYB42
increased the expression of nitrogen stress-related genes. These results indicated that
CmMYB42
acts as a positive regulator to improve the tolerance of
Chrysanthemum
to low-nitrogen stress, providing a theoretical basis for further exploring the molecular mechanism of low-nitrogen tolerance in
Chrysanthemum
. |
| doi_str_mv | 10.1007/s00344-023-10940-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2858498249</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2858498249</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-d4b75b9a8766cbed8bba52cf34d5640e5bb2187e181807236435ad525af0d07a3</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EEqXwA6wssTb4GTvLEvGoVEAqZcHKOInTpmrsYqeg_j2GILFjNRrNPTNzLwDnBF8SjOVVxJhxjjBliOCcY0QOwIhwJpAiWB6CEZaUIClycQxOYlxjTFIjR-Bt4uCczhl6eL2Gi2BcrEK77Vvv4K2peh9g0aURp3DabYP_sBHO_Cd6bPvgl9bB5z7YGOHCb2yCKwtbB4tV2Efj-pXtdt0pOGrMJtqz3zoGL7c3i-IezZ7upsVkhipG8h7VvJSizI2SWVaVtlZlaQStGsZrkXFsRVlSoqQliqjkhWXJm6kFFabBNZaGjcHFsDd9-b6zsddrvwsundRUCcVzRXmeVHRQVcHHGGyjt6HtTNhrgvV3knpIUqck9U-SmiSIDVBMYre04W_1P9QXgEl1HA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2858498249</pqid></control><display><type>article</type><title>An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum</title><source>SpringerNature Journals</source><creator>Ma, Rui ; Yang, Sen ; Liu, Yanhong ; Sun, Xia ; Wang, Wenli ; Zheng, Chengshu</creator><creatorcontrib>Ma, Rui ; Yang, Sen ; Liu, Yanhong ; Sun, Xia ; Wang, Wenli ; Zheng, Chengshu</creatorcontrib><description>Plant roots can sense the nitrogen level in the environment and change the expression of related genes to maintain normal life activities. However, their regulatory mechanism in
Chrysanthemum
has not been fully clarified. In this study, an R2R3-MYB transcription factor
CmMYB42
was isolated from
Chrysanthemum
‘Jinba.’ Tissue expression analysis showed that the gene was mainly expressed in the root and could rapidly up-regulate the expression of
CmMYB42
under low-nitrogen conditions. Subcellular localization analysis showed that
CmMYB42
was located in the nucleus. Under low-nitrogen stress, overexpressing
CmMYB42 Chrysanthemum
’s total root length, lateral root number, plant height, and biomass accumulation were significantly higher than wild type and slowed down the damage to photosynthetic system and improved NO
3
−
content and nitrogen metabolism assimilation enzyme activity. In addition, we also found that
CmMYB42
increased the expression of nitrogen stress-related genes. These results indicated that
CmMYB42
acts as a positive regulator to improve the tolerance of
Chrysanthemum
to low-nitrogen stress, providing a theoretical basis for further exploring the molecular mechanism of low-nitrogen tolerance in
Chrysanthemum
.</description><identifier>ISSN: 0721-7595</identifier><identifier>EISSN: 1435-8107</identifier><identifier>DOI: 10.1007/s00344-023-10940-1</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Chrysanthemum ; Enzymatic activity ; Enzyme activity ; Gene expression ; Genes ; Life Sciences ; Localization ; Molecular modelling ; Nitrogen ; Nitrogen metabolism ; Plant Anatomy/Development ; Plant Physiology ; Plant roots ; Plant Sciences ; Regulatory mechanisms (biology) ; Transcription factors</subject><ispartof>Journal of plant growth regulation, 2023-09, Vol.42 (9), p.5600-5614</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-d4b75b9a8766cbed8bba52cf34d5640e5bb2187e181807236435ad525af0d07a3</citedby><cites>FETCH-LOGICAL-c319t-d4b75b9a8766cbed8bba52cf34d5640e5bb2187e181807236435ad525af0d07a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00344-023-10940-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00344-023-10940-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids></links><search><creatorcontrib>Ma, Rui</creatorcontrib><creatorcontrib>Yang, Sen</creatorcontrib><creatorcontrib>Liu, Yanhong</creatorcontrib><creatorcontrib>Sun, Xia</creatorcontrib><creatorcontrib>Wang, Wenli</creatorcontrib><creatorcontrib>Zheng, Chengshu</creatorcontrib><title>An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum</title><title>Journal of plant growth regulation</title><addtitle>J Plant Growth Regul</addtitle><description>Plant roots can sense the nitrogen level in the environment and change the expression of related genes to maintain normal life activities. However, their regulatory mechanism in
Chrysanthemum
has not been fully clarified. In this study, an R2R3-MYB transcription factor
CmMYB42
was isolated from
Chrysanthemum
‘Jinba.’ Tissue expression analysis showed that the gene was mainly expressed in the root and could rapidly up-regulate the expression of
CmMYB42
under low-nitrogen conditions. Subcellular localization analysis showed that
CmMYB42
was located in the nucleus. Under low-nitrogen stress, overexpressing
CmMYB42 Chrysanthemum
’s total root length, lateral root number, plant height, and biomass accumulation were significantly higher than wild type and slowed down the damage to photosynthetic system and improved NO
3
−
content and nitrogen metabolism assimilation enzyme activity. In addition, we also found that
CmMYB42
increased the expression of nitrogen stress-related genes. These results indicated that
CmMYB42
acts as a positive regulator to improve the tolerance of
Chrysanthemum
to low-nitrogen stress, providing a theoretical basis for further exploring the molecular mechanism of low-nitrogen tolerance in
Chrysanthemum
.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Chrysanthemum</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Molecular modelling</subject><subject>Nitrogen</subject><subject>Nitrogen metabolism</subject><subject>Plant Anatomy/Development</subject><subject>Plant Physiology</subject><subject>Plant roots</subject><subject>Plant Sciences</subject><subject>Regulatory mechanisms (biology)</subject><subject>Transcription factors</subject><issn>0721-7595</issn><issn>1435-8107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtOwzAQRS0EEqXwA6wssTb4GTvLEvGoVEAqZcHKOInTpmrsYqeg_j2GILFjNRrNPTNzLwDnBF8SjOVVxJhxjjBliOCcY0QOwIhwJpAiWB6CEZaUIClycQxOYlxjTFIjR-Bt4uCczhl6eL2Gi2BcrEK77Vvv4K2peh9g0aURp3DabYP_sBHO_Cd6bPvgl9bB5z7YGOHCb2yCKwtbB4tV2Efj-pXtdt0pOGrMJtqz3zoGL7c3i-IezZ7upsVkhipG8h7VvJSizI2SWVaVtlZlaQStGsZrkXFsRVlSoqQliqjkhWXJm6kFFabBNZaGjcHFsDd9-b6zsddrvwsundRUCcVzRXmeVHRQVcHHGGyjt6HtTNhrgvV3knpIUqck9U-SmiSIDVBMYre04W_1P9QXgEl1HA</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Ma, Rui</creator><creator>Yang, Sen</creator><creator>Liu, Yanhong</creator><creator>Sun, Xia</creator><creator>Wang, Wenli</creator><creator>Zheng, Chengshu</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20230901</creationdate><title>An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum</title><author>Ma, Rui ; Yang, Sen ; Liu, Yanhong ; Sun, Xia ; Wang, Wenli ; Zheng, Chengshu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-d4b75b9a8766cbed8bba52cf34d5640e5bb2187e181807236435ad525af0d07a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Chrysanthemum</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Molecular modelling</topic><topic>Nitrogen</topic><topic>Nitrogen metabolism</topic><topic>Plant Anatomy/Development</topic><topic>Plant Physiology</topic><topic>Plant roots</topic><topic>Plant Sciences</topic><topic>Regulatory mechanisms (biology)</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Rui</creatorcontrib><creatorcontrib>Yang, Sen</creatorcontrib><creatorcontrib>Liu, Yanhong</creatorcontrib><creatorcontrib>Sun, Xia</creatorcontrib><creatorcontrib>Wang, Wenli</creatorcontrib><creatorcontrib>Zheng, Chengshu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Journal of plant growth regulation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Rui</au><au>Yang, Sen</au><au>Liu, Yanhong</au><au>Sun, Xia</au><au>Wang, Wenli</au><au>Zheng, Chengshu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum</atitle><jtitle>Journal of plant growth regulation</jtitle><stitle>J Plant Growth Regul</stitle><date>2023-09-01</date><risdate>2023</risdate><volume>42</volume><issue>9</issue><spage>5600</spage><epage>5614</epage><pages>5600-5614</pages><issn>0721-7595</issn><eissn>1435-8107</eissn><abstract>Plant roots can sense the nitrogen level in the environment and change the expression of related genes to maintain normal life activities. However, their regulatory mechanism in
Chrysanthemum
has not been fully clarified. In this study, an R2R3-MYB transcription factor
CmMYB42
was isolated from
Chrysanthemum
‘Jinba.’ Tissue expression analysis showed that the gene was mainly expressed in the root and could rapidly up-regulate the expression of
CmMYB42
under low-nitrogen conditions. Subcellular localization analysis showed that
CmMYB42
was located in the nucleus. Under low-nitrogen stress, overexpressing
CmMYB42 Chrysanthemum
’s total root length, lateral root number, plant height, and biomass accumulation were significantly higher than wild type and slowed down the damage to photosynthetic system and improved NO
3
−
content and nitrogen metabolism assimilation enzyme activity. In addition, we also found that
CmMYB42
increased the expression of nitrogen stress-related genes. These results indicated that
CmMYB42
acts as a positive regulator to improve the tolerance of
Chrysanthemum
to low-nitrogen stress, providing a theoretical basis for further exploring the molecular mechanism of low-nitrogen tolerance in
Chrysanthemum
.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00344-023-10940-1</doi><tpages>15</tpages></addata></record> |
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| subjects | Agriculture Biomedical and Life Sciences Chrysanthemum Enzymatic activity Enzyme activity Gene expression Genes Life Sciences Localization Molecular modelling Nitrogen Nitrogen metabolism Plant Anatomy/Development Plant Physiology Plant roots Plant Sciences Regulatory mechanisms (biology) Transcription factors |
| title | An R2R3-MYB Transcription Factor CmMYB42 Improves Low-Nitrogen Stress Tolerance in Chrysanthemum |
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