Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion
Although regenerative therapy and bioartificial tissues and organs require a sufficient number of human cells, current cell expansion processes are accompanied by accumulation of senescent cells that are related to deterioration of cellular functions and induction of senescence-associated secretory...
Gespeichert in:
| Veröffentlicht in: | Journal of artificial organs 2023-09, Vol.26 (3), p.246-250 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 250 |
|---|---|
| container_issue | 3 |
| container_start_page | 246 |
| container_title | Journal of artificial organs |
| container_volume | 26 |
| creator | Ashiba, Keisuke Mino, Koki Okido, Yui Sato, Kiyoshi Kawakami, Hiroyoshi |
| description | Although regenerative therapy and bioartificial tissues and organs require a sufficient number of human cells, current cell expansion processes are accompanied by accumulation of senescent cells that are related to deterioration of cellular functions and induction of senescence-associated secretory phenotype (SASP). Therefore, suppression of replicative senescence during expansion is one of the crucial issues for dissemination of regenerative medicine. We herein developed dual drug-encapsulated liposomal nanoparticles (LNPs) to suppress cellular senescence in human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and natural killer (NK) cells by removal of dysfunctional mitochondria from the senescent cells. We found that LNP treatment reduced senescent makers; downregulation of p21 expression and reduction of SA-β-Gal activity in both cells provably due to mitophagy reactivation in the cells. Moreover, SASP secretion in hAT-MSCs and tumor cytotoxicity in NK cells were also improved upon LNP treatments. These findings may contribute to the production of highly effective expanded cells for regenerative medicine and bioartificial tissues and organs. |
| doi_str_mv | 10.1007/s10047-022-01356-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_journals_2849339642</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2849339642</sourcerecordid><originalsourceid>FETCH-LOGICAL-p180t-fec19d21f0b5b2b7c54cf0c4604e7e1626bea8723c367b148f07dce44b70e6a93</originalsourceid><addsrcrecordid>eNpFkUtPwzAQhC0EoqXwBzggS5wN60fi5IgqXlIlDsDZcpxNSeU6wW5Q--9JKYjL7kozmpXmI-SSww0H0LdpnEozEIIBl1nOtkdkynNeMihBHY-3koppIcoJOUtpBcB1puGUTGRWFipTYkr8KwZMDoNDGtF1XxjbsKTVjtaD9bSOw5KNou3T4O0Ga-rbvkvdetSCDV1v46Z1HhNtuki9jUtkyVmP9GNY20Adek9x29uQ2i6ck5PG-oQXv3tG3h_u3-ZPbPHy-Dy_W7CeF7BhDTpe1oI3UGWVqLTLlGvAqRwUauS5yCu0hRbSyVxXXBUN6NqhUpUGzG0pZ-T6kNvH7nPAtDGrbohhfGlEoUopy1yJ0XX16xqqNdamj-3axp35K2c0yIMh9ftSMP7HcDB7BOaAwIwIzA8Cs5Xffxl5RQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2849339642</pqid></control><display><type>article</type><title>Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion</title><source>SpringerLink Journals - AutoHoldings</source><creator>Ashiba, Keisuke ; Mino, Koki ; Okido, Yui ; Sato, Kiyoshi ; Kawakami, Hiroyoshi</creator><creatorcontrib>Ashiba, Keisuke ; Mino, Koki ; Okido, Yui ; Sato, Kiyoshi ; Kawakami, Hiroyoshi</creatorcontrib><description>Although regenerative therapy and bioartificial tissues and organs require a sufficient number of human cells, current cell expansion processes are accompanied by accumulation of senescent cells that are related to deterioration of cellular functions and induction of senescence-associated secretory phenotype (SASP). Therefore, suppression of replicative senescence during expansion is one of the crucial issues for dissemination of regenerative medicine. We herein developed dual drug-encapsulated liposomal nanoparticles (LNPs) to suppress cellular senescence in human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and natural killer (NK) cells by removal of dysfunctional mitochondria from the senescent cells. We found that LNP treatment reduced senescent makers; downregulation of p21 expression and reduction of SA-β-Gal activity in both cells provably due to mitophagy reactivation in the cells. Moreover, SASP secretion in hAT-MSCs and tumor cytotoxicity in NK cells were also improved upon LNP treatments. These findings may contribute to the production of highly effective expanded cells for regenerative medicine and bioartificial tissues and organs.</description><identifier>ISSN: 1434-7229</identifier><identifier>EISSN: 1619-0904</identifier><identifier>DOI: 10.1007/s10047-022-01356-x</identifier><identifier>PMID: 35984542</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Adipose tissue ; Biomedical Engineering and Bioengineering ; Brief Communication ; Cardiac Surgery ; Cytotoxicity ; Encapsulation ; Medicine ; Medicine & Public Health ; Mesenchymal stem cells ; Mitochondria ; Nanoparticles ; Natural killer cells ; Nephrology ; Organs ; Phenotypes ; Regenerative medicine ; Senescence ; Stem cells ; Tissue engineering</subject><ispartof>Journal of artificial organs, 2023-09, Vol.26 (3), p.246-250</ispartof><rights>The Japanese Society for Artificial Organs 2022</rights><rights>2022. The Japanese Society for Artificial Organs.</rights><rights>The Japanese Society for Artificial Organs 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p180t-fec19d21f0b5b2b7c54cf0c4604e7e1626bea8723c367b148f07dce44b70e6a93</cites><orcidid>0000-0002-1268-7386</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10047-022-01356-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10047-022-01356-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35984542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ashiba, Keisuke</creatorcontrib><creatorcontrib>Mino, Koki</creatorcontrib><creatorcontrib>Okido, Yui</creatorcontrib><creatorcontrib>Sato, Kiyoshi</creatorcontrib><creatorcontrib>Kawakami, Hiroyoshi</creatorcontrib><title>Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion</title><title>Journal of artificial organs</title><addtitle>J Artif Organs</addtitle><addtitle>J Artif Organs</addtitle><description>Although regenerative therapy and bioartificial tissues and organs require a sufficient number of human cells, current cell expansion processes are accompanied by accumulation of senescent cells that are related to deterioration of cellular functions and induction of senescence-associated secretory phenotype (SASP). Therefore, suppression of replicative senescence during expansion is one of the crucial issues for dissemination of regenerative medicine. We herein developed dual drug-encapsulated liposomal nanoparticles (LNPs) to suppress cellular senescence in human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and natural killer (NK) cells by removal of dysfunctional mitochondria from the senescent cells. We found that LNP treatment reduced senescent makers; downregulation of p21 expression and reduction of SA-β-Gal activity in both cells provably due to mitophagy reactivation in the cells. Moreover, SASP secretion in hAT-MSCs and tumor cytotoxicity in NK cells were also improved upon LNP treatments. These findings may contribute to the production of highly effective expanded cells for regenerative medicine and bioartificial tissues and organs.</description><subject>Adipose tissue</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Brief Communication</subject><subject>Cardiac Surgery</subject><subject>Cytotoxicity</subject><subject>Encapsulation</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal stem cells</subject><subject>Mitochondria</subject><subject>Nanoparticles</subject><subject>Natural killer cells</subject><subject>Nephrology</subject><subject>Organs</subject><subject>Phenotypes</subject><subject>Regenerative medicine</subject><subject>Senescence</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>1434-7229</issn><issn>1619-0904</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpFkUtPwzAQhC0EoqXwBzggS5wN60fi5IgqXlIlDsDZcpxNSeU6wW5Q--9JKYjL7kozmpXmI-SSww0H0LdpnEozEIIBl1nOtkdkynNeMihBHY-3koppIcoJOUtpBcB1puGUTGRWFipTYkr8KwZMDoNDGtF1XxjbsKTVjtaD9bSOw5KNou3T4O0Ga-rbvkvdetSCDV1v46Z1HhNtuki9jUtkyVmP9GNY20Adek9x29uQ2i6ck5PG-oQXv3tG3h_u3-ZPbPHy-Dy_W7CeF7BhDTpe1oI3UGWVqLTLlGvAqRwUauS5yCu0hRbSyVxXXBUN6NqhUpUGzG0pZ-T6kNvH7nPAtDGrbohhfGlEoUopy1yJ0XX16xqqNdamj-3axp35K2c0yIMh9ftSMP7HcDB7BOaAwIwIzA8Cs5Xffxl5RQ</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Ashiba, Keisuke</creator><creator>Mino, Koki</creator><creator>Okido, Yui</creator><creator>Sato, Kiyoshi</creator><creator>Kawakami, Hiroyoshi</creator><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-1268-7386</orcidid></search><sort><creationdate>20230901</creationdate><title>Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion</title><author>Ashiba, Keisuke ; Mino, Koki ; Okido, Yui ; Sato, Kiyoshi ; Kawakami, Hiroyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p180t-fec19d21f0b5b2b7c54cf0c4604e7e1626bea8723c367b148f07dce44b70e6a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adipose tissue</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Brief Communication</topic><topic>Cardiac Surgery</topic><topic>Cytotoxicity</topic><topic>Encapsulation</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal stem cells</topic><topic>Mitochondria</topic><topic>Nanoparticles</topic><topic>Natural killer cells</topic><topic>Nephrology</topic><topic>Organs</topic><topic>Phenotypes</topic><topic>Regenerative medicine</topic><topic>Senescence</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashiba, Keisuke</creatorcontrib><creatorcontrib>Mino, Koki</creatorcontrib><creatorcontrib>Okido, Yui</creatorcontrib><creatorcontrib>Sato, Kiyoshi</creatorcontrib><creatorcontrib>Kawakami, Hiroyoshi</creatorcontrib><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashiba, Keisuke</au><au>Mino, Koki</au><au>Okido, Yui</au><au>Sato, Kiyoshi</au><au>Kawakami, Hiroyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion</atitle><jtitle>Journal of artificial organs</jtitle><stitle>J Artif Organs</stitle><addtitle>J Artif Organs</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>26</volume><issue>3</issue><spage>246</spage><epage>250</epage><pages>246-250</pages><issn>1434-7229</issn><eissn>1619-0904</eissn><abstract>Although regenerative therapy and bioartificial tissues and organs require a sufficient number of human cells, current cell expansion processes are accompanied by accumulation of senescent cells that are related to deterioration of cellular functions and induction of senescence-associated secretory phenotype (SASP). Therefore, suppression of replicative senescence during expansion is one of the crucial issues for dissemination of regenerative medicine. We herein developed dual drug-encapsulated liposomal nanoparticles (LNPs) to suppress cellular senescence in human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and natural killer (NK) cells by removal of dysfunctional mitochondria from the senescent cells. We found that LNP treatment reduced senescent makers; downregulation of p21 expression and reduction of SA-β-Gal activity in both cells provably due to mitophagy reactivation in the cells. Moreover, SASP secretion in hAT-MSCs and tumor cytotoxicity in NK cells were also improved upon LNP treatments. These findings may contribute to the production of highly effective expanded cells for regenerative medicine and bioartificial tissues and organs.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>35984542</pmid><doi>10.1007/s10047-022-01356-x</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1268-7386</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1434-7229 |
| ispartof | Journal of artificial organs, 2023-09, Vol.26 (3), p.246-250 |
| issn | 1434-7229 1619-0904 |
| language | eng |
| recordid | cdi_proquest_journals_2849339642 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Adipose tissue Biomedical Engineering and Bioengineering Brief Communication Cardiac Surgery Cytotoxicity Encapsulation Medicine Medicine & Public Health Mesenchymal stem cells Mitochondria Nanoparticles Natural killer cells Nephrology Organs Phenotypes Regenerative medicine Senescence Stem cells Tissue engineering |
| title | Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T08%3A40%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Senescence%20recovering%20by%20dual%20drug-encapsulated%20liposomal%20nanoparticles%20for%20large-scale%20human%20cell%20expansion&rft.jtitle=Journal%20of%20artificial%20organs&rft.au=Ashiba,%20Keisuke&rft.date=2023-09-01&rft.volume=26&rft.issue=3&rft.spage=246&rft.epage=250&rft.pages=246-250&rft.issn=1434-7229&rft.eissn=1619-0904&rft_id=info:doi/10.1007/s10047-022-01356-x&rft_dat=%3Cproquest_pubme%3E2849339642%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2849339642&rft_id=info:pmid/35984542&rfr_iscdi=true |