Transport and Fate of Ureolytic Sporosarcina pasteurii in Saturated Sand Columns: Experiments and Modelling
Abstarct Despite a broad application of ureolytic bacteria in many bioremediation and biocementation processes, very limited studies have reported their transport and retention behaviors under various physical–chemical–biological conditions. In this study, we report transport and retention of Sporos...
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description | Abstarct
Despite a broad application of ureolytic bacteria in many bioremediation and biocementation processes, very limited studies have reported their transport and retention behaviors under various physical–chemical–biological conditions. In this study, we report transport and retention of
Sporosarcina pasteurii
in saturated sand, based on a series of column breakthrough experiments under different conditions including ionic strengths (ISs: 0.5 mM–1 M), flow velocity (50, 100, 200 cm/h), bacteria optical density (OD
600
= 1.0, 0.48), column length (280 mm, 150 mm), and changes in IS conditions (0.5 M CaCl
2
or deionised water). We use a two-site kinetic model, representing (1) attachment on grain surfaces, and (2) straining at crevices and constrictions, to quantify and predict the bacterial attachment and straining. Model parameters were calibrated by tracer (NaCl) breakthrough curves (BTCs) and bacteria BTCs at different IS/velocity conditions. The model was then applied to successfully predict the bacteria BTCs at lower initial bacteria density (OD
600
= 0.48) and for shorter column lengths (150 mm). We demonstrated that higher ionic strength (from 0.5 to 1000 mM) dramatically enhanced the retention efficiency of
S. pasteurii
through an enhancement of attachment (from 9.4 to 69.6%) and straining (from 8.1 to 34.2%), whilst the bacterial survival and the urease activity were unaffected at high IS conditions (500 and 1000 mM NaCl) within 5 h. Increasing flow velocity (from 50 to 200 cm/h) caused a decrease in attachment (from 39.5 to 22.4%) and decrease in straining (from 40.5 to 19.3%) as a result of the increased hydrodynamic shear forces, which tends to reduce the attachment at the secondary minimum and decrease the extent of flow stagnation regions for straining. Lower initial bacteria OD
600
(from 1.0 to 0.48) enhanced the attachment (from 31.8 to 40.9%) and the straining (from 22.9 to 42.2%) as a result of reducing the site-blockage effect. In addition, 0.5 M CaCl
2
with a stronger IS increased the retention of in the column, whilst deionised water with a lower IS caused bacterial release. These findings provide useful information for a better understanding of the transport and fate of
Sporosarcina pasteurii
in saturated soil, and can be used to optimise bioaugmentation strategy and cementation efficiency for soil improvement.
Article Highlights
Transport of
S. pasteurii
in sands is highly affected by ionic strength, flow velocity, bacteria den |
doi_str_mv | 10.1007/s11242-023-01973-x |
format | Article |
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Despite a broad application of ureolytic bacteria in many bioremediation and biocementation processes, very limited studies have reported their transport and retention behaviors under various physical–chemical–biological conditions. In this study, we report transport and retention of
Sporosarcina pasteurii
in saturated sand, based on a series of column breakthrough experiments under different conditions including ionic strengths (ISs: 0.5 mM–1 M), flow velocity (50, 100, 200 cm/h), bacteria optical density (OD
600
= 1.0, 0.48), column length (280 mm, 150 mm), and changes in IS conditions (0.5 M CaCl
2
or deionised water). We use a two-site kinetic model, representing (1) attachment on grain surfaces, and (2) straining at crevices and constrictions, to quantify and predict the bacterial attachment and straining. Model parameters were calibrated by tracer (NaCl) breakthrough curves (BTCs) and bacteria BTCs at different IS/velocity conditions. The model was then applied to successfully predict the bacteria BTCs at lower initial bacteria density (OD
600
= 0.48) and for shorter column lengths (150 mm). We demonstrated that higher ionic strength (from 0.5 to 1000 mM) dramatically enhanced the retention efficiency of
S. pasteurii
through an enhancement of attachment (from 9.4 to 69.6%) and straining (from 8.1 to 34.2%), whilst the bacterial survival and the urease activity were unaffected at high IS conditions (500 and 1000 mM NaCl) within 5 h. Increasing flow velocity (from 50 to 200 cm/h) caused a decrease in attachment (from 39.5 to 22.4%) and decrease in straining (from 40.5 to 19.3%) as a result of the increased hydrodynamic shear forces, which tends to reduce the attachment at the secondary minimum and decrease the extent of flow stagnation regions for straining. Lower initial bacteria OD
600
(from 1.0 to 0.48) enhanced the attachment (from 31.8 to 40.9%) and the straining (from 22.9 to 42.2%) as a result of reducing the site-blockage effect. In addition, 0.5 M CaCl
2
with a stronger IS increased the retention of in the column, whilst deionised water with a lower IS caused bacterial release. These findings provide useful information for a better understanding of the transport and fate of
Sporosarcina pasteurii
in saturated soil, and can be used to optimise bioaugmentation strategy and cementation efficiency for soil improvement.
Article Highlights
Transport of
S. pasteurii
in sands is highly affected by ionic strength, flow velocity, bacteria density, and even column size
Straining was enhanced (from 8.1% to 34.2%) if increasing IS (from 0.5 to 500 mM) without affecting bacterial survival
Bacteria coagulation among 2–3 bacterial cells occurs under ISs of 500 and 1000 mM without forming large flocculation</description><identifier>ISSN: 0169-3913</identifier><identifier>EISSN: 1573-1634</identifier><identifier>DOI: 10.1007/s11242-023-01973-x</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Attachment ; Bacteria ; Bioremediation ; Calcium chloride ; Civil Engineering ; Classical and Continuum Physics ; Coagulation ; Earth and Environmental Science ; Earth Sciences ; Flow velocity ; Geotechnical Engineering & Applied Earth Sciences ; Hydrogeology ; Hydrology/Water Resources ; Industrial Chemistry/Chemical Engineering ; Optical density ; Retention ; Saturated soils ; Shear forces ; Soil improvement ; Survival</subject><ispartof>Transport in porous media, 2023-09, Vol.149 (2), p.599-624</ispartof><rights>The Author(s) 2023</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-752c675825e8737cb3fa1ffb4f4b4317e8e530b8dbaa6dfd235d73da332f2d33</citedby><cites>FETCH-LOGICAL-c363t-752c675825e8737cb3fa1ffb4f4b4317e8e530b8dbaa6dfd235d73da332f2d33</cites><orcidid>0000-0002-2379-7521</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11242-023-01973-x$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11242-023-01973-x$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sang, Guijie</creatorcontrib><creatorcontrib>Lunn, Rebecca J.</creatorcontrib><creatorcontrib>El Mountassir, Grainne</creatorcontrib><creatorcontrib>Minto, James M.</creatorcontrib><title>Transport and Fate of Ureolytic Sporosarcina pasteurii in Saturated Sand Columns: Experiments and Modelling</title><title>Transport in porous media</title><addtitle>Transp Porous Med</addtitle><description>Abstarct
Despite a broad application of ureolytic bacteria in many bioremediation and biocementation processes, very limited studies have reported their transport and retention behaviors under various physical–chemical–biological conditions. In this study, we report transport and retention of
Sporosarcina pasteurii
in saturated sand, based on a series of column breakthrough experiments under different conditions including ionic strengths (ISs: 0.5 mM–1 M), flow velocity (50, 100, 200 cm/h), bacteria optical density (OD
600
= 1.0, 0.48), column length (280 mm, 150 mm), and changes in IS conditions (0.5 M CaCl
2
or deionised water). We use a two-site kinetic model, representing (1) attachment on grain surfaces, and (2) straining at crevices and constrictions, to quantify and predict the bacterial attachment and straining. Model parameters were calibrated by tracer (NaCl) breakthrough curves (BTCs) and bacteria BTCs at different IS/velocity conditions. The model was then applied to successfully predict the bacteria BTCs at lower initial bacteria density (OD
600
= 0.48) and for shorter column lengths (150 mm). We demonstrated that higher ionic strength (from 0.5 to 1000 mM) dramatically enhanced the retention efficiency of
S. pasteurii
through an enhancement of attachment (from 9.4 to 69.6%) and straining (from 8.1 to 34.2%), whilst the bacterial survival and the urease activity were unaffected at high IS conditions (500 and 1000 mM NaCl) within 5 h. Increasing flow velocity (from 50 to 200 cm/h) caused a decrease in attachment (from 39.5 to 22.4%) and decrease in straining (from 40.5 to 19.3%) as a result of the increased hydrodynamic shear forces, which tends to reduce the attachment at the secondary minimum and decrease the extent of flow stagnation regions for straining. Lower initial bacteria OD
600
(from 1.0 to 0.48) enhanced the attachment (from 31.8 to 40.9%) and the straining (from 22.9 to 42.2%) as a result of reducing the site-blockage effect. In addition, 0.5 M CaCl
2
with a stronger IS increased the retention of in the column, whilst deionised water with a lower IS caused bacterial release. These findings provide useful information for a better understanding of the transport and fate of
Sporosarcina pasteurii
in saturated soil, and can be used to optimise bioaugmentation strategy and cementation efficiency for soil improvement.
Article Highlights
Transport of
S. pasteurii
in sands is highly affected by ionic strength, flow velocity, bacteria density, and even column size
Straining was enhanced (from 8.1% to 34.2%) if increasing IS (from 0.5 to 500 mM) without affecting bacterial survival
Bacteria coagulation among 2–3 bacterial cells occurs under ISs of 500 and 1000 mM without forming large flocculation</description><subject>Attachment</subject><subject>Bacteria</subject><subject>Bioremediation</subject><subject>Calcium chloride</subject><subject>Civil Engineering</subject><subject>Classical and Continuum Physics</subject><subject>Coagulation</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Flow velocity</subject><subject>Geotechnical Engineering & Applied Earth Sciences</subject><subject>Hydrogeology</subject><subject>Hydrology/Water Resources</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Optical density</subject><subject>Retention</subject><subject>Saturated soils</subject><subject>Shear forces</subject><subject>Soil improvement</subject><subject>Survival</subject><issn>0169-3913</issn><issn>1573-1634</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kDFPwzAQhS0EEqXwB5gsMQdsXxK7bKhqAamIoWW2nNiuUlIn2I7U_ntMg8TGdCfd-97dPYRuKbmnhPCHQCnLWUYYZITOOGSHMzShRWpoCfk5mhBazjKYUbhEVyHsCEmYyCfoc-OVC33nI1ZO46WKBncWf3jTtcfY1HidZl1Qvm6cwr0K0Qy-aXDj8FrFwSe9Tl1C51077F14xItDb3yzNy6Gk-dbp03bNm57jS6saoO5-a1TtFkuNvOXbPX-_Dp_WmU1lBAzXrC65IVghREceF2BVdTaKrd5lQPlRpgCSCV0pVSprWZQaA5aATDLNMAU3Y22ve--BhOi3HWDd2mjZCInJQcmRFKxUVWn94I3VvbpaOWPkhL5k6kcM5UpU3nKVB4SBCMUkthtjf-z_of6BiWje_M</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Sang, Guijie</creator><creator>Lunn, Rebecca J.</creator><creator>El Mountassir, Grainne</creator><creator>Minto, James M.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L6V</scope><scope>M7S</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><orcidid>https://orcid.org/0000-0002-2379-7521</orcidid></search><sort><creationdate>20230901</creationdate><title>Transport and Fate of Ureolytic Sporosarcina pasteurii in Saturated Sand Columns: Experiments and Modelling</title><author>Sang, Guijie ; Lunn, Rebecca J. ; El Mountassir, Grainne ; Minto, James M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-752c675825e8737cb3fa1ffb4f4b4317e8e530b8dbaa6dfd235d73da332f2d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Attachment</topic><topic>Bacteria</topic><topic>Bioremediation</topic><topic>Calcium chloride</topic><topic>Civil Engineering</topic><topic>Classical and Continuum Physics</topic><topic>Coagulation</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Flow velocity</topic><topic>Geotechnical Engineering & Applied Earth Sciences</topic><topic>Hydrogeology</topic><topic>Hydrology/Water Resources</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Optical density</topic><topic>Retention</topic><topic>Saturated soils</topic><topic>Shear forces</topic><topic>Soil improvement</topic><topic>Survival</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sang, Guijie</creatorcontrib><creatorcontrib>Lunn, Rebecca J.</creatorcontrib><creatorcontrib>El Mountassir, Grainne</creatorcontrib><creatorcontrib>Minto, James M.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><jtitle>Transport in porous media</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sang, Guijie</au><au>Lunn, Rebecca J.</au><au>El Mountassir, Grainne</au><au>Minto, James M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transport and Fate of Ureolytic Sporosarcina pasteurii in Saturated Sand Columns: Experiments and Modelling</atitle><jtitle>Transport in porous media</jtitle><stitle>Transp Porous Med</stitle><date>2023-09-01</date><risdate>2023</risdate><volume>149</volume><issue>2</issue><spage>599</spage><epage>624</epage><pages>599-624</pages><issn>0169-3913</issn><eissn>1573-1634</eissn><abstract>Abstarct
Despite a broad application of ureolytic bacteria in many bioremediation and biocementation processes, very limited studies have reported their transport and retention behaviors under various physical–chemical–biological conditions. In this study, we report transport and retention of
Sporosarcina pasteurii
in saturated sand, based on a series of column breakthrough experiments under different conditions including ionic strengths (ISs: 0.5 mM–1 M), flow velocity (50, 100, 200 cm/h), bacteria optical density (OD
600
= 1.0, 0.48), column length (280 mm, 150 mm), and changes in IS conditions (0.5 M CaCl
2
or deionised water). We use a two-site kinetic model, representing (1) attachment on grain surfaces, and (2) straining at crevices and constrictions, to quantify and predict the bacterial attachment and straining. Model parameters were calibrated by tracer (NaCl) breakthrough curves (BTCs) and bacteria BTCs at different IS/velocity conditions. The model was then applied to successfully predict the bacteria BTCs at lower initial bacteria density (OD
600
= 0.48) and for shorter column lengths (150 mm). We demonstrated that higher ionic strength (from 0.5 to 1000 mM) dramatically enhanced the retention efficiency of
S. pasteurii
through an enhancement of attachment (from 9.4 to 69.6%) and straining (from 8.1 to 34.2%), whilst the bacterial survival and the urease activity were unaffected at high IS conditions (500 and 1000 mM NaCl) within 5 h. Increasing flow velocity (from 50 to 200 cm/h) caused a decrease in attachment (from 39.5 to 22.4%) and decrease in straining (from 40.5 to 19.3%) as a result of the increased hydrodynamic shear forces, which tends to reduce the attachment at the secondary minimum and decrease the extent of flow stagnation regions for straining. Lower initial bacteria OD
600
(from 1.0 to 0.48) enhanced the attachment (from 31.8 to 40.9%) and the straining (from 22.9 to 42.2%) as a result of reducing the site-blockage effect. In addition, 0.5 M CaCl
2
with a stronger IS increased the retention of in the column, whilst deionised water with a lower IS caused bacterial release. These findings provide useful information for a better understanding of the transport and fate of
Sporosarcina pasteurii
in saturated soil, and can be used to optimise bioaugmentation strategy and cementation efficiency for soil improvement.
Article Highlights
Transport of
S. pasteurii
in sands is highly affected by ionic strength, flow velocity, bacteria density, and even column size
Straining was enhanced (from 8.1% to 34.2%) if increasing IS (from 0.5 to 500 mM) without affecting bacterial survival
Bacteria coagulation among 2–3 bacterial cells occurs under ISs of 500 and 1000 mM without forming large flocculation</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11242-023-01973-x</doi><tpages>26</tpages><orcidid>https://orcid.org/0000-0002-2379-7521</orcidid><oa>free_for_read</oa></addata></record> |
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source | Springer Nature - Complete Springer Journals |
subjects | Attachment Bacteria Bioremediation Calcium chloride Civil Engineering Classical and Continuum Physics Coagulation Earth and Environmental Science Earth Sciences Flow velocity Geotechnical Engineering & Applied Earth Sciences Hydrogeology Hydrology/Water Resources Industrial Chemistry/Chemical Engineering Optical density Retention Saturated soils Shear forces Soil improvement Survival |
title | Transport and Fate of Ureolytic Sporosarcina pasteurii in Saturated Sand Columns: Experiments and Modelling |
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