Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast
Fission yeast, Schizosaccharomyces pombe, possesses eight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measur...
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| Veröffentlicht in: | Journal of general and applied microbiology 2022, Vol.68(6), pp.270-277 |
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| container_title | Journal of general and applied microbiology |
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| creator | Maruyama, Teppei Hayashi, Kanako Matsui, Kotaro Maekawa, Yasukichi Shimasaki, Takafumi Ohtsuka, Hokuto Shigeaki, Saitoh Aiba, Hirofumi |
| description | Fission yeast, Schizosaccharomyces pombe, possesses eight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measured. Under normal growth condition containing 2% glucose, ∆ght5 and ∆ght2 mutants showed large and small decrease in glucose uptake activity, respectively. On the other hand, the other deletion mutants did not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2, the other hexose transporters do not play a significant role in glucose uptake. To understand the relevance between glucose uptake and lifespan regulation, we measured the chronological lifespan of each hexose transporter deletion mutant, and found that only ∆ght5 mutant showed a significant lifespan extension. Based on these results we showed that Ght5 is mainly involved in the glucose uptake in Schizosaccharomyces pombe, and suggested that the ∆ght5 mutant has prolonged lifespan due to physiological changes similar to calorie restriction. |
| doi_str_mv | 10.2323/jgam.2022.05.006 |
| format | Article |
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In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measured. Under normal growth condition containing 2% glucose, ∆ght5 and ∆ght2 mutants showed large and small decrease in glucose uptake activity, respectively. On the other hand, the other deletion mutants did not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2, the other hexose transporters do not play a significant role in glucose uptake. To understand the relevance between glucose uptake and lifespan regulation, we measured the chronological lifespan of each hexose transporter deletion mutant, and found that only ∆ght5 mutant showed a significant lifespan extension. Based on these results we showed that Ght5 is mainly involved in the glucose uptake in Schizosaccharomyces pombe, and suggested that the ∆ght5 mutant has prolonged lifespan due to physiological changes similar to calorie restriction.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.2022.05.006</identifier><identifier>PMID: 35781263</identifier><language>eng</language><publisher>Japan: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>Deletion ; Deletion mutant ; Fission ; fission yeast ; Ght5 ; Glucose ; Glucose transporter ; Growth conditions ; Hexose ; Hexose transporter ; Life span ; lifespan ; Longevity ; Monosaccharide Transport Proteins - genetics ; Mutants ; Nutrient deficiency ; Schizosaccharomyces - genetics ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Yeast ; Yeasts</subject><ispartof>The Journal of General and Applied Microbiology, 2022, Vol.68(6), pp.270-277</ispartof><rights>2022 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</rights><rights>Copyright Japan Science and Technology Agency 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-5bd1452c1a87913b926831a71b92f391492065950d56518fe17370cb1afae2743</citedby><cites>FETCH-LOGICAL-c555t-5bd1452c1a87913b926831a71b92f391492065950d56518fe17370cb1afae2743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,1879,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35781263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maruyama, Teppei</creatorcontrib><creatorcontrib>Hayashi, Kanako</creatorcontrib><creatorcontrib>Matsui, Kotaro</creatorcontrib><creatorcontrib>Maekawa, Yasukichi</creatorcontrib><creatorcontrib>Shimasaki, Takafumi</creatorcontrib><creatorcontrib>Ohtsuka, Hokuto</creatorcontrib><creatorcontrib>Shigeaki, Saitoh</creatorcontrib><creatorcontrib>Aiba, Hirofumi</creatorcontrib><title>Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast</title><title>Journal of general and applied microbiology</title><addtitle>J. Gen. Appl. Microbiol.</addtitle><description>Fission yeast, Schizosaccharomyces pombe, possesses eight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measured. Under normal growth condition containing 2% glucose, ∆ght5 and ∆ght2 mutants showed large and small decrease in glucose uptake activity, respectively. On the other hand, the other deletion mutants did not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2, the other hexose transporters do not play a significant role in glucose uptake. To understand the relevance between glucose uptake and lifespan regulation, we measured the chronological lifespan of each hexose transporter deletion mutant, and found that only ∆ght5 mutant showed a significant lifespan extension. Based on these results we showed that Ght5 is mainly involved in the glucose uptake in Schizosaccharomyces pombe, and suggested that the ∆ght5 mutant has prolonged lifespan due to physiological changes similar to calorie restriction.</description><subject>Deletion</subject><subject>Deletion mutant</subject><subject>Fission</subject><subject>fission yeast</subject><subject>Ght5</subject><subject>Glucose</subject><subject>Glucose transporter</subject><subject>Growth conditions</subject><subject>Hexose</subject><subject>Hexose transporter</subject><subject>Life span</subject><subject>lifespan</subject><subject>Longevity</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>Mutants</subject><subject>Nutrient deficiency</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins - genetics</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0022-1260</issn><issn>1349-8037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc1vEzEQxS1ERUPKnROyxHmDP9b7cUQRBaRKXOjZmjizG4eNvdheaBF_fG2lRBxsjz2_90Z6JuQtZxshhfxwHOG0EUyIDVMbxpoXZMVl3Vcdk-1LsmK5U3HRsGvyOsYjY7IRXf2KXEvVdvldrsjf7QECmITB_oFkvaN-oAd88BFpCuDi7ENu0hEdRmodTQekvyz-jgUsF3MI3vnJj9bARCc7II0zOApuT8dpMcVpmRP8wCIfbIxlyiNCTDfkaoAp4pvnc03ubz99336p7r59_rr9eFcZpVSq1G7PayUMh67tudz1oukkh5bnapA9r3vBGtUrtleN4t2AvJUtMzsOA6Boa7km78--c_A_F4xJH_0SXB6pRSdboaTKa03YmTLBxxhw0HOwJwiPmjNd4tYlbl3i1kzpHHeWvHs2XnYn3F8E__LNwO0ZOMYEI14ACMmaCc-OTaebsv3nfAFM_h6NTj4BSjaVHg</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Maruyama, Teppei</creator><creator>Hayashi, Kanako</creator><creator>Matsui, Kotaro</creator><creator>Maekawa, Yasukichi</creator><creator>Shimasaki, Takafumi</creator><creator>Ohtsuka, Hokuto</creator><creator>Shigeaki, Saitoh</creator><creator>Aiba, Hirofumi</creator><general>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20220101</creationdate><title>Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast</title><author>Maruyama, Teppei ; Hayashi, Kanako ; Matsui, Kotaro ; Maekawa, Yasukichi ; Shimasaki, Takafumi ; Ohtsuka, Hokuto ; Shigeaki, Saitoh ; Aiba, Hirofumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-5bd1452c1a87913b926831a71b92f391492065950d56518fe17370cb1afae2743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Deletion</topic><topic>Deletion mutant</topic><topic>Fission</topic><topic>fission yeast</topic><topic>Ght5</topic><topic>Glucose</topic><topic>Glucose transporter</topic><topic>Growth conditions</topic><topic>Hexose</topic><topic>Hexose transporter</topic><topic>Life span</topic><topic>lifespan</topic><topic>Longevity</topic><topic>Monosaccharide Transport Proteins - genetics</topic><topic>Mutants</topic><topic>Nutrient deficiency</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins - genetics</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maruyama, Teppei</creatorcontrib><creatorcontrib>Hayashi, Kanako</creatorcontrib><creatorcontrib>Matsui, Kotaro</creatorcontrib><creatorcontrib>Maekawa, Yasukichi</creatorcontrib><creatorcontrib>Shimasaki, Takafumi</creatorcontrib><creatorcontrib>Ohtsuka, Hokuto</creatorcontrib><creatorcontrib>Shigeaki, Saitoh</creatorcontrib><creatorcontrib>Aiba, Hirofumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maruyama, Teppei</au><au>Hayashi, Kanako</au><au>Matsui, Kotaro</au><au>Maekawa, Yasukichi</au><au>Shimasaki, Takafumi</au><au>Ohtsuka, Hokuto</au><au>Shigeaki, Saitoh</au><au>Aiba, Hirofumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>68</volume><issue>6</issue><spage>270</spage><epage>277</epage><pages>270-277</pages><artnum>2022.05.006</artnum><issn>0022-1260</issn><eissn>1349-8037</eissn><abstract>Fission yeast, Schizosaccharomyces pombe, possesses eight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucose uptake, a glucose uptake assay system was established and the actual glucose uptake activity of each hexose transporter-deletion mutant was measured. Under normal growth condition containing 2% glucose, ∆ght5 and ∆ght2 mutants showed large and small decrease in glucose uptake activity, respectively. On the other hand, the other deletion mutants did not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2, the other hexose transporters do not play a significant role in glucose uptake. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Deletion Deletion mutant Fission fission yeast Ght5 Glucose Glucose transporter Growth conditions Hexose Hexose transporter Life span lifespan Longevity Monosaccharide Transport Proteins - genetics Mutants Nutrient deficiency Schizosaccharomyces - genetics Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Yeast Yeasts |
| title | Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast |
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