Prevalence of pulpy kidney disease and antimicrobial sensitivity profile of Clostridium perfringens type D recovered from enteric intoxicated diarrheic goat of different age and gender in District Tharparkar, Sindh Pakistan

Present study has been carried out to assess the occurrence of pulpy kidney disease and assessment of antimicrobial sensitivity profile of Clostridium perfringens type D against various antibiotics. Total 100 fecal samples were collected from diarrheic goat in aseptic polyethylene sachets, and then further processed for identification and isolation of Clostridium perfringens type D by different laboratory techniques. Positive samples were processed for detecting their antibiotic susceptibility pattern, through 2-fold micro broth dilution method by using MIC. From total collected samples only 45 samples were found positive. The age wise occurrence of pulpy kidney disease was 11.11 % in 1 month, 33.33% in 6 months, 40.00 % in 1 year, and 15.56 % in 2 year of goat. However; males were 44.44% while females were 55.56% positive for pulpy kidney disease. Among positive samples, 01 sample shown resistant at 16µg/ml and 01 sample at the concentration of 32µg/ml against Gentamycin. Although for Tylosin titrate, 01 sample shown resistant at 16µg/ml, 01 sample at 32µg/ml and 01 sample at the concentration of 64µg/ml. Moreover, for Trimethoprim/Sulphamethaxzol all samples were detected sensitive. Likewise, 01 sample shown resistant at 32µg/ml and 01 sample at the concentration of 64µg/ml concentration of Amoxicillin. In the same way 01 sample shown resistant at 32µg/ml concentration of Chloramphenicol used in this study. This study has been evaluated the occurrence of pulpy kidney disease and also provide the best treatment options and preventive measures against the outbreak of this fatal infection which will prove beneficial for farmers to save their animals from this fatal intoxication.