Phytochemical Screening and Antioxidant potential of Lepidagathis spinosa Wight ex Nees., Whole plant - In vitro method
Aim: To assess the phytoconstituents, total flavonoid, phenolic content and in vitro antioxidant activity of Lepidagathis spinosa wight ex Nees. (L.spinosa). Objective: To assess the phytochemical content and antioxidant activity of L. spinosa with different solvents like petroleum ether, chloroform...
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| Veröffentlicht in: | Research journal of pharmacy and technology 2023-03, Vol.16 (3), p.1395-1400 |
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| creator | Ponnusamy, Sutha Balakrishnan, Sangameswaran |
| description | Aim: To assess the phytoconstituents, total flavonoid, phenolic content and in vitro antioxidant activity of Lepidagathis spinosa wight ex Nees. (L.spinosa). Objective: To assess the phytochemical content and antioxidant activity of L. spinosa with different solvents like petroleum ether, chloroform, ethyl acetate and hydro alcohol extracts. Total phenolic and flavonoid contents were assessed using spectrophotometric method. The total antioxidant capacity and free radical scavenging activities of various extracts were estimated against DPPH, Hydroxy radical and Nitric oxide radical scavenging assays. Results: The hydro alcohol extract of the whole shrub revealed the maximal polyphenol content when correlated with the other solvent extracts. Total phenolic content of the hydro alcohol extract was found to be 33.53 of GAE/g dry weight of samples. Flavonoid content was found to be 19.63333 mg of quercetin equivalents/g dry weight of the extract. All the extracts were exposed significant antioxidant activity and hydro alcohol extract showing highest antioxidant ability in association with the polyphenol contents. Based on the IC50 values, hydro alcohol extract of the plant revealed the potent DPPH, Hydroxy radical, Nitric oxide radical scavenging ability. Conclusion: This study suggests that hydro alcohol extract of L. spinosa could be a potential source of natural antioxidant and justifies its traditional use in ethnomedicine. |
| doi_str_mv | 10.52711/0974-360X.2023.00230 |
| format | Article |
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(L.spinosa). Objective: To assess the phytochemical content and antioxidant activity of L. spinosa with different solvents like petroleum ether, chloroform, ethyl acetate and hydro alcohol extracts. Total phenolic and flavonoid contents were assessed using spectrophotometric method. The total antioxidant capacity and free radical scavenging activities of various extracts were estimated against DPPH, Hydroxy radical and Nitric oxide radical scavenging assays. Results: The hydro alcohol extract of the whole shrub revealed the maximal polyphenol content when correlated with the other solvent extracts. Total phenolic content of the hydro alcohol extract was found to be 33.53 of GAE/g dry weight of samples. Flavonoid content was found to be 19.63333 mg of quercetin equivalents/g dry weight of the extract. All the extracts were exposed significant antioxidant activity and hydro alcohol extract showing highest antioxidant ability in association with the polyphenol contents. Based on the IC50 values, hydro alcohol extract of the plant revealed the potent DPPH, Hydroxy radical, Nitric oxide radical scavenging ability. Conclusion: This study suggests that hydro alcohol extract of L. spinosa could be a potential source of natural antioxidant and justifies its traditional use in ethnomedicine.</description><identifier>ISSN: 0974-3618</identifier><identifier>EISSN: 0974-360X</identifier><identifier>EISSN: 0974-306X</identifier><identifier>DOI: 10.52711/0974-360X.2023.00230</identifier><language>eng</language><publisher>Raipur: A&V Publications</publisher><subject>Alcohol ; Antioxidants ; Calibration ; Ethanol ; Flavonoids ; Metabolites ; Methods ; Nitric oxide ; Phytochemicals ; Reagents ; Sulfur</subject><ispartof>Research journal of pharmacy and technology, 2023-03, Vol.16 (3), p.1395-1400</ispartof><rights>Copyright A&V Publications Mar 2023</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c144t-8a7bb8b1c5de095f0614c04604083d29d6ef3ec09ec68d90ba7e7291c4f369903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ponnusamy, Sutha</creatorcontrib><creatorcontrib>Balakrishnan, Sangameswaran</creatorcontrib><title>Phytochemical Screening and Antioxidant potential of Lepidagathis spinosa Wight ex Nees., Whole plant - In vitro method</title><title>Research journal of pharmacy and technology</title><description>Aim: To assess the phytoconstituents, total flavonoid, phenolic content and in vitro antioxidant activity of Lepidagathis spinosa wight ex Nees. (L.spinosa). Objective: To assess the phytochemical content and antioxidant activity of L. spinosa with different solvents like petroleum ether, chloroform, ethyl acetate and hydro alcohol extracts. Total phenolic and flavonoid contents were assessed using spectrophotometric method. The total antioxidant capacity and free radical scavenging activities of various extracts were estimated against DPPH, Hydroxy radical and Nitric oxide radical scavenging assays. Results: The hydro alcohol extract of the whole shrub revealed the maximal polyphenol content when correlated with the other solvent extracts. Total phenolic content of the hydro alcohol extract was found to be 33.53 of GAE/g dry weight of samples. Flavonoid content was found to be 19.63333 mg of quercetin equivalents/g dry weight of the extract. All the extracts were exposed significant antioxidant activity and hydro alcohol extract showing highest antioxidant ability in association with the polyphenol contents. Based on the IC50 values, hydro alcohol extract of the plant revealed the potent DPPH, Hydroxy radical, Nitric oxide radical scavenging ability. Conclusion: This study suggests that hydro alcohol extract of L. spinosa could be a potential source of natural antioxidant and justifies its traditional use in ethnomedicine.</description><subject>Alcohol</subject><subject>Antioxidants</subject><subject>Calibration</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Nitric oxide</subject><subject>Phytochemicals</subject><subject>Reagents</subject><subject>Sulfur</subject><issn>0974-3618</issn><issn>0974-360X</issn><issn>0974-306X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNo9kF1LwzAUhoMoOOZ-ghDw1s6kTdv0cgw_BkMFlXkX0vR0zeiSmkTd_r3tJjsX5-PlPefAg9A1JdM0zim9I0XOoiQjn9OYxMmU9ImcodFJPj_1lF-iifcb0kfG05jxEfp9bfbBqga2WskWvykHYLRZY2kqPDNB252upAm4swH6sffYGi-h69W1DI322HfaWC_xSq-bgGGHnwH89BavGtsC7tphO8ILg390cBZvITS2ukIXtWw9TP7rGH083L_Pn6Lly-NiPltGijIWIi7zsuQlVWkFpEhrklGmCMsIIzyp4qLKoE5AkQJUxquClDKHPC6oYnWSFQVJxujmeLdz9usbfBAb--1M_1LEPKE8zhilvSs9upSz3juoRef0Vrq9oEQcMIuBoRh4igGzOGBO_gAGUHBX</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Ponnusamy, Sutha</creator><creator>Balakrishnan, Sangameswaran</creator><general>A&V Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04S</scope><scope>04W</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20230301</creationdate><title>Phytochemical Screening and Antioxidant potential of Lepidagathis spinosa Wight ex Nees., Whole plant - In vitro method</title><author>Ponnusamy, Sutha ; Balakrishnan, Sangameswaran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c144t-8a7bb8b1c5de095f0614c04604083d29d6ef3ec09ec68d90ba7e7291c4f369903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alcohol</topic><topic>Antioxidants</topic><topic>Calibration</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Metabolites</topic><topic>Methods</topic><topic>Nitric oxide</topic><topic>Phytochemicals</topic><topic>Reagents</topic><topic>Sulfur</topic><toplevel>online_resources</toplevel><creatorcontrib>Ponnusamy, Sutha</creatorcontrib><creatorcontrib>Balakrishnan, Sangameswaran</creatorcontrib><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Business</collection><collection>India Database: Science & Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Research journal of pharmacy and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ponnusamy, Sutha</au><au>Balakrishnan, Sangameswaran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical Screening and Antioxidant potential of Lepidagathis spinosa Wight ex Nees., Whole plant - In vitro method</atitle><jtitle>Research journal of pharmacy and technology</jtitle><date>2023-03-01</date><risdate>2023</risdate><volume>16</volume><issue>3</issue><spage>1395</spage><epage>1400</epage><pages>1395-1400</pages><issn>0974-3618</issn><eissn>0974-360X</eissn><eissn>0974-306X</eissn><abstract>Aim: To assess the phytoconstituents, total flavonoid, phenolic content and in vitro antioxidant activity of Lepidagathis spinosa wight ex Nees. (L.spinosa). Objective: To assess the phytochemical content and antioxidant activity of L. spinosa with different solvents like petroleum ether, chloroform, ethyl acetate and hydro alcohol extracts. Total phenolic and flavonoid contents were assessed using spectrophotometric method. The total antioxidant capacity and free radical scavenging activities of various extracts were estimated against DPPH, Hydroxy radical and Nitric oxide radical scavenging assays. Results: The hydro alcohol extract of the whole shrub revealed the maximal polyphenol content when correlated with the other solvent extracts. Total phenolic content of the hydro alcohol extract was found to be 33.53 of GAE/g dry weight of samples. Flavonoid content was found to be 19.63333 mg of quercetin equivalents/g dry weight of the extract. All the extracts were exposed significant antioxidant activity and hydro alcohol extract showing highest antioxidant ability in association with the polyphenol contents. Based on the IC50 values, hydro alcohol extract of the plant revealed the potent DPPH, Hydroxy radical, Nitric oxide radical scavenging ability. Conclusion: This study suggests that hydro alcohol extract of L. spinosa could be a potential source of natural antioxidant and justifies its traditional use in ethnomedicine.</abstract><cop>Raipur</cop><pub>A&V Publications</pub><doi>10.52711/0974-360X.2023.00230</doi><tpages>6</tpages></addata></record> |
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| subjects | Alcohol Antioxidants Calibration Ethanol Flavonoids Metabolites Methods Nitric oxide Phytochemicals Reagents Sulfur |
| title | Phytochemical Screening and Antioxidant potential of Lepidagathis spinosa Wight ex Nees., Whole plant - In vitro method |
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