Molecular cloning and production of recombinant Pcal_0672, a family GH57 glycoside hydrolase from Pyrobaculum calidifontis

An open reading frame of putative gene (designated as Pcal_0672), that belongs to family GH57 (UniProt acc # A3MTY1 and GenBank # ABO08098.1), was found in Pyrobaculum calidifontis . It consists of 485 amino acid residues and was amplified, cloned, and produced in Escherichia coli using T7 expressio...

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Veröffentlicht in:	Biológia 2023-07, Vol.78 (7), p.1861-1874
Hauptverfasser:	Mehboob, Sumaira, Ali, Ramzan, Bashir, Shahzad, Ahmad, Nasir, Ahmad, Tuba, Butt, Hamama Islam, Rashid, Naeem
Format:	Artikel
Sprache:	eng
Schlagworte:	Aggregates Amino acids Amylases Autoclaving Biomedical and Life Sciences Cell Biology Cloning E coli Enzymes Glucan 1,4-a-maltohydrolase Glycosidases Glycoside hydrolase Hydrothermal vents Life Sciences Lysates Microbiology Original Article Plant Sciences Pyrobaculum calidifontis Residues Starch Zoology
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