Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.）
Fragrance is one of the important traits for quality improvement in rice, which is mainly controlled by the Badh2 gene encoding a betaine aldehyde dehydrogenase on chromosome 8. The mutation of Badh2 gene can increase the content of 2-acetyl-1-pyrroline（2-AP） and promote the fragrance production in...
Gespeichert in:
| Veröffentlicht in: | Zuo wu xue bao 2023-01, Vol.49 (8), p.2144 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 8 |
| container_start_page | 2144 |
| container_title | Zuo wu xue bao |
| container_volume | 49 |
| creator | Wei, Xin-Yu Zeng, Yue-Hui Yang, Wang-Xing Xiao, Chang-Chun Hou, Xin-Po Huang, Jian-Hong Zou, Wen-Guang Xu, Xu-Ming |
| description | Fragrance is one of the important traits for quality improvement in rice, which is mainly controlled by the Badh2 gene encoding a betaine aldehyde dehydrogenase on chromosome 8. The mutation of Badh2 gene can increase the content of 2-acetyl-1-pyrroline（2-AP） and promote the fragrance production in rice. In this study, the Badh2 gene in Mingtai B（MTB）, an elite maintainer line of Indica hybrid rice showing high eating quality bred by Sanming Academy of Agricultural Sciences, was edited and knocked out by using CRISPR-Cas9 technology. Two T0 transgenic lines carrying homozygous mutation on the loci of Badh2 were generated, and 48 T1 individuals derived from these two plants were genotyped by PCR amplification and sequencing analysis. A mutant line named MTB-badh2, which had a single T nucleotide insertion in the second exon of Badh2 without the vector skeleton, was finally obtained. In our study, the expression of Badh2 and the content of 2-AP were determined by semi-quantitative RT-PCR, qRT-PCR, and gas chrom |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2828913089</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2828913089</sourcerecordid><originalsourceid>FETCH-proquest_journals_28289130893</originalsourceid><addsrcrecordid>eNqNjMFKw0AURWehYNH-wwPXkXGmDZmtUVFQlLb78pq8TJ7EmXZmUohrF_pP_af-ghH8AFcXzjncEzGRM5NnembkmZjGyBsplcq1nucT8XlLe-r89p1cAt9Ay7bNdj12nAZoAtqAo2BXc4VQPi-hY0ewGYBqTuws3GDdKrA00j7-gnLxuHxdZCVGA4mq1vnO22G8gMAVHQ9fL2H4QIiYeI_wdHU8fF-I0wa7SNO_PReX93er8iHbBr_rKab1m--DG9VaFaow11oWRv-v-gGoz1Lm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2828913089</pqid></control><display><type>article</type><title>Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.）</title><source>DOAJ Directory of Open Access Journals</source><creator>Wei, Xin-Yu ; Zeng, Yue-Hui ; Yang, Wang-Xing ; Xiao, Chang-Chun ; Hou, Xin-Po ; Huang, Jian-Hong ; Zou, Wen-Guang ; Xu, Xu-Ming</creator><creatorcontrib>Wei, Xin-Yu ; Zeng, Yue-Hui ; Yang, Wang-Xing ; Xiao, Chang-Chun ; Hou, Xin-Po ; Huang, Jian-Hong ; Zou, Wen-Guang ; Xu, Xu-Ming</creatorcontrib><description>Fragrance is one of the important traits for quality improvement in rice, which is mainly controlled by the Badh2 gene encoding a betaine aldehyde dehydrogenase on chromosome 8. The mutation of Badh2 gene can increase the content of 2-acetyl-1-pyrroline（2-AP） and promote the fragrance production in rice. In this study, the Badh2 gene in Mingtai B（MTB）, an elite maintainer line of Indica hybrid rice showing high eating quality bred by Sanming Academy of Agricultural Sciences, was edited and knocked out by using CRISPR-Cas9 technology. Two T0 transgenic lines carrying homozygous mutation on the loci of Badh2 were generated, and 48 T1 individuals derived from these two plants were genotyped by PCR amplification and sequencing analysis. A mutant line named MTB-badh2, which had a single T nucleotide insertion in the second exon of Badh2 without the vector skeleton, was finally obtained. In our study, the expression of Badh2 and the content of 2-AP were determined by semi-quantitative RT-PCR, qRT-PCR, and gas chrom</description><identifier>ISSN: 0496-3490</identifier><language>chi</language><publisher>Beijing: Chinese Academy of Agricultural Sciences (CAAS)</publisher><subject>Agricultural sciences ; Aldehyde dehydrogenase ; Aldehydes ; Chromosome 8 ; CRISPR ; Crop production ; Crop yield ; Deoxyribonucleic acid ; DNA ; Eating quality ; Fingerprints ; Fragrances ; Gas chromatography ; Genetic diversity ; Grain ; Loci ; Mass spectrometry ; Mass spectroscopy ; Mutants ; Mutation ; Nucleotides ; Oryza sativa indica ; Phenotypes ; Plant breeding ; Polymerase chain reaction ; Quality control ; Rice ; Sequence analysis ; Technology utilization</subject><ispartof>Zuo wu xue bao, 2023-01, Vol.49 (8), p.2144</ispartof><rights>Copyright Chinese Academy of Agricultural Sciences (CAAS) 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Wei, Xin-Yu</creatorcontrib><creatorcontrib>Zeng, Yue-Hui</creatorcontrib><creatorcontrib>Yang, Wang-Xing</creatorcontrib><creatorcontrib>Xiao, Chang-Chun</creatorcontrib><creatorcontrib>Hou, Xin-Po</creatorcontrib><creatorcontrib>Huang, Jian-Hong</creatorcontrib><creatorcontrib>Zou, Wen-Guang</creatorcontrib><creatorcontrib>Xu, Xu-Ming</creatorcontrib><title>Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.）</title><title>Zuo wu xue bao</title><description>Fragrance is one of the important traits for quality improvement in rice, which is mainly controlled by the Badh2 gene encoding a betaine aldehyde dehydrogenase on chromosome 8. The mutation of Badh2 gene can increase the content of 2-acetyl-1-pyrroline（2-AP） and promote the fragrance production in rice. In this study, the Badh2 gene in Mingtai B（MTB）, an elite maintainer line of Indica hybrid rice showing high eating quality bred by Sanming Academy of Agricultural Sciences, was edited and knocked out by using CRISPR-Cas9 technology. Two T0 transgenic lines carrying homozygous mutation on the loci of Badh2 were generated, and 48 T1 individuals derived from these two plants were genotyped by PCR amplification and sequencing analysis. A mutant line named MTB-badh2, which had a single T nucleotide insertion in the second exon of Badh2 without the vector skeleton, was finally obtained. In our study, the expression of Badh2 and the content of 2-AP were determined by semi-quantitative RT-PCR, qRT-PCR, and gas chrom</description><subject>Agricultural sciences</subject><subject>Aldehyde dehydrogenase</subject><subject>Aldehydes</subject><subject>Chromosome 8</subject><subject>CRISPR</subject><subject>Crop production</subject><subject>Crop yield</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Eating quality</subject><subject>Fingerprints</subject><subject>Fragrances</subject><subject>Gas chromatography</subject><subject>Genetic diversity</subject><subject>Grain</subject><subject>Loci</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Oryza sativa indica</subject><subject>Phenotypes</subject><subject>Plant breeding</subject><subject>Polymerase chain reaction</subject><subject>Quality control</subject><subject>Rice</subject><subject>Sequence analysis</subject><subject>Technology utilization</subject><issn>0496-3490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNjMFKw0AURWehYNH-wwPXkXGmDZmtUVFQlLb78pq8TJ7EmXZmUohrF_pP_af-ghH8AFcXzjncEzGRM5NnembkmZjGyBsplcq1nucT8XlLe-r89p1cAt9Ay7bNdj12nAZoAtqAo2BXc4VQPi-hY0ewGYBqTuws3GDdKrA00j7-gnLxuHxdZCVGA4mq1vnO22G8gMAVHQ9fL2H4QIiYeI_wdHU8fF-I0wa7SNO_PReX93er8iHbBr_rKab1m--DG9VaFaow11oWRv-v-gGoz1Lm</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Wei, Xin-Yu</creator><creator>Zeng, Yue-Hui</creator><creator>Yang, Wang-Xing</creator><creator>Xiao, Chang-Chun</creator><creator>Hou, Xin-Po</creator><creator>Huang, Jian-Hong</creator><creator>Zou, Wen-Guang</creator><creator>Xu, Xu-Ming</creator><general>Chinese Academy of Agricultural Sciences (CAAS)</general><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20230101</creationdate><title>Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.）</title><author>Wei, Xin-Yu ; Zeng, Yue-Hui ; Yang, Wang-Xing ; Xiao, Chang-Chun ; Hou, Xin-Po ; Huang, Jian-Hong ; Zou, Wen-Guang ; Xu, Xu-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_28289130893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2023</creationdate><topic>Agricultural sciences</topic><topic>Aldehyde dehydrogenase</topic><topic>Aldehydes</topic><topic>Chromosome 8</topic><topic>CRISPR</topic><topic>Crop production</topic><topic>Crop yield</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Eating quality</topic><topic>Fingerprints</topic><topic>Fragrances</topic><topic>Gas chromatography</topic><topic>Genetic diversity</topic><topic>Grain</topic><topic>Loci</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Oryza sativa indica</topic><topic>Phenotypes</topic><topic>Plant breeding</topic><topic>Polymerase chain reaction</topic><topic>Quality control</topic><topic>Rice</topic><topic>Sequence analysis</topic><topic>Technology utilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Xin-Yu</creatorcontrib><creatorcontrib>Zeng, Yue-Hui</creatorcontrib><creatorcontrib>Yang, Wang-Xing</creatorcontrib><creatorcontrib>Xiao, Chang-Chun</creatorcontrib><creatorcontrib>Hou, Xin-Po</creatorcontrib><creatorcontrib>Huang, Jian-Hong</creatorcontrib><creatorcontrib>Zou, Wen-Guang</creatorcontrib><creatorcontrib>Xu, Xu-Ming</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Zuo wu xue bao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Xin-Yu</au><au>Zeng, Yue-Hui</au><au>Yang, Wang-Xing</au><au>Xiao, Chang-Chun</au><au>Hou, Xin-Po</au><au>Huang, Jian-Hong</au><au>Zou, Wen-Guang</au><au>Xu, Xu-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.）</atitle><jtitle>Zuo wu xue bao</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>49</volume><issue>8</issue><spage>2144</spage><pages>2144-</pages><issn>0496-3490</issn><abstract>Fragrance is one of the important traits for quality improvement in rice, which is mainly controlled by the Badh2 gene encoding a betaine aldehyde dehydrogenase on chromosome 8. The mutation of Badh2 gene can increase the content of 2-acetyl-1-pyrroline（2-AP） and promote the fragrance production in rice. In this study, the Badh2 gene in Mingtai B（MTB）, an elite maintainer line of Indica hybrid rice showing high eating quality bred by Sanming Academy of Agricultural Sciences, was edited and knocked out by using CRISPR-Cas9 technology. Two T0 transgenic lines carrying homozygous mutation on the loci of Badh2 were generated, and 48 T1 individuals derived from these two plants were genotyped by PCR amplification and sequencing analysis. A mutant line named MTB-badh2, which had a single T nucleotide insertion in the second exon of Badh2 without the vector skeleton, was finally obtained. In our study, the expression of Badh2 and the content of 2-AP were determined by semi-quantitative RT-PCR, qRT-PCR, and gas chrom</abstract><cop>Beijing</cop><pub>Chinese Academy of Agricultural Sciences (CAAS)</pub></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0496-3490 |
| ispartof | Zuo wu xue bao, 2023-01, Vol.49 (8), p.2144 |
| issn | 0496-3490 |
| language | chi |
| recordid | cdi_proquest_journals_2828913089 |
| source | DOAJ Directory of Open Access Journals |
| subjects | Agricultural sciences Aldehyde dehydrogenase Aldehydes Chromosome 8 CRISPR Crop production Crop yield Deoxyribonucleic acid DNA Eating quality Fingerprints Fragrances Gas chromatography Genetic diversity Grain Loci Mass spectrometry Mass spectroscopy Mutants Mutation Nucleotides Oryza sativa indica Phenotypes Plant breeding Polymerase chain reaction Quality control Rice Sequence analysis Technology utilization |
| title | Development of high-quality fragrant indica CMS line by editing Badh2 gene using CRISPR-Cas9 technology in rice（Oryza sativa L.） |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T16%3A18%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20high-quality%20fragrant%20indica%20CMS%20line%20by%20editing%20Badh2%20gene%20using%20CRISPR-Cas9%20technology%20in%20rice%EF%BC%88Oryza%20sativa%20L.%EF%BC%89&rft.jtitle=Zuo%20wu%20xue%20bao&rft.au=Wei,%20Xin-Yu&rft.date=2023-01-01&rft.volume=49&rft.issue=8&rft.spage=2144&rft.pages=2144-&rft.issn=0496-3490&rft_id=info:doi/&rft_dat=%3Cproquest%3E2828913089%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2828913089&rft_id=info:pmid/&rfr_iscdi=true |