Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis

Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis

Background: Symptoms and signs of acute viral and bacterial rhinosinusitis that are not specific cause difficulties in diagnostic enforcement, causing difficulties in administering therapy, so often doctors provide antibiotic therapy on acute viral rhinosinusitis. Irrelevant antibiotics will cause f...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bangladesh journal of medical science (Ibn Sina Trust) 2022-02, Vol.21 (2), p.311-317
Hauptverfasser: Widi Wardani, Andriana Tjitria, Purwanto, Bambang, DonoIndarto, -, Wasita, Brian, Cilmiaty AR, Risya
Format: Artikel
Sprache:eng
Schlagworte:
Antibiotics
Ethanol
Rhinitis
Sinusitis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 317
container_issue 2
container_start_page 311
container_title Bangladesh journal of medical science (Ibn Sina Trust)
container_volume 21
creator Widi Wardani, Andriana Tjitria
Purwanto, Bambang
DonoIndarto, -
Wasita, Brian
Cilmiaty AR, Risya
description Background: Symptoms and signs of acute viral and bacterial rhinosinusitis that are not specific cause difficulties in diagnostic enforcement, causing difficulties in administering therapy, so often doctors provide antibiotic therapy on acute viral rhinosinusitis. Irrelevant antibiotics will cause frequent antibiotic resistance. Adjuvant therapy is required to prevent infections that continue to become chronic, shorten the duration of infection, and avoid unwanted complications. Moringa leaves (M. oleifera)contains antioxidant components that can help rebalance redox metabolism and reduce excess free radicals so as to prevent oxidative stress. Method: posttest only control group research design. Male strain Sprague Dawley white rat was maintained and treated at the Nutrition Laboratory of the Center for Food and Nutrition Studies of Gadjah Mada University (UGM). Mice were divided into 4 groups. Group 1: standard feed., group 2: standard feed and staphylococcus-induced aureus, group 3: standard feed, Staphylococcus aureus-induced given amoxicillin dose of 27 mg/day, group 4: standard feed, Staphylococcus aureus-induced was given amoxicillin dose of 27 mg/day and given (EEDK) 200 mg/KgBW/day. Data analysis Of Normality test using Shapiro-Wilk Test and homogeneity using Levene’s Test obtained results p>0.05. Then conducted One Way AnovaTest obtained p
doi_str_mv 10.3329/bjms.v21i2.58063
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2826130543</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2826130543</sourcerecordid><originalsourceid>FETCH-LOGICAL-c271t-4d24b3404fcbea0e960088946b5a103d95f3c8ffec44d928bb73f9ae9c8832673</originalsourceid><addsrcrecordid>eNotkUFOwzAQRSMEElXpnqUltqQ4tpPYy6q0UKkFqZR15Dg2dZXExXYqehrOwEU4E07LbGak_2a-Rj-KbhM4xhixh3LXuPEBJRqNUwozfBENEMyzGCLGLsOMEI5JjpLraOTcDobKIEwZHUTfM6Wk8MAosDJWtx8cLCVXYOa3vDU1mH15y3u9BWtZdUJWQT_I2p02Kn4PNi_z-PcH8LYCj9IJq_deBzrIi1bVvGm4N_YIprIOSzqc4R686bZzYNUJ43jwrWTd8xPReQnWW90a1wPaa3cTXSleOzn678PofT7bTJ_j5evTYjpZxgLliY9JhUiJCSRKlJJDycJ_lDKSlSlPIK5YqrCg_auEVAzRssyxYlwyQSlGWY6H0d357t6az046X-xMZ9tgWSCKsgTDlOBAwTMlrHHOSlXsrW64PRYJLPokij6J4pREcUoC_wHu8H6R</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2826130543</pqid></control><display><type>article</type><title>Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis</title><source>DOAJ Directory of Open Access Journals</source><creator>Widi Wardani, Andriana Tjitria ; Purwanto, Bambang ; DonoIndarto, - ; Wasita, Brian ; Cilmiaty AR, Risya</creator><creatorcontrib>Widi Wardani, Andriana Tjitria ; Purwanto, Bambang ; DonoIndarto, - ; Wasita, Brian ; Cilmiaty AR, Risya</creatorcontrib><description>Background: Symptoms and signs of acute viral and bacterial rhinosinusitis that are not specific cause difficulties in diagnostic enforcement, causing difficulties in administering therapy, so often doctors provide antibiotic therapy on acute viral rhinosinusitis. Irrelevant antibiotics will cause frequent antibiotic resistance. Adjuvant therapy is required to prevent infections that continue to become chronic, shorten the duration of infection, and avoid unwanted complications. Moringa leaves (M. oleifera)contains antioxidant components that can help rebalance redox metabolism and reduce excess free radicals so as to prevent oxidative stress. Method: posttest only control group research design. Male strain Sprague Dawley white rat was maintained and treated at the Nutrition Laboratory of the Center for Food and Nutrition Studies of Gadjah Mada University (UGM). Mice were divided into 4 groups. Group 1: standard feed., group 2: standard feed and staphylococcus-induced aureus, group 3: standard feed, Staphylococcus aureus-induced given amoxicillin dose of 27 mg/day, group 4: standard feed, Staphylococcus aureus-induced was given amoxicillin dose of 27 mg/day and given (EEDK) 200 mg/KgBW/day. Data analysis Of Normality test using Shapiro-Wilk Test and homogeneity using Levene’s Test obtained results p&gt;0.05. Then conducted One Way AnovaTest obtained p&lt;0.05 and continued with post-hoc LSD test. The data is analyzed using SPSS 25.0 for Windows. Result: MDA test Anova obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD all groups (p-value) &lt; 0.05, indicated a significant mean difference in average MDA levels among all groups. The lowest mean difference in MDA levels in group K with P3 was 0.92 pg/mL; while P1 with P3 has the largest average MDA rate difference of 7.47 pg/mL. TNF-α Anova test obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD of all groups (p-value= 0.000) &lt; 0.05, there is a meaningful difference in the average level of TNF-α between all groups. The smallest average difference in TNF-α levels in group K with P3 was 0.31 pg/mL; while P1 with P3 has the largest average TNF-α rate of 5.01 pg/mL. Description of inflammation of the mucosa cavum rice and sinus K score 0; P1 score 3; P2 score 2; P3 score 1. Conclusion: Moringa leaf ethanol extract in this study can be used as adjuvant therapy because it can lower MDA and TNF-α levels in mice induced with Staphylococcus aureus along with amoxicillin standard therapy. Extract ethanol moringa leaves in this study can be used as adjuvant therapy because it can lower the score of inflammatory cells better than by only given standard therapy only Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 311-317</description><identifier>ISSN: 2223-4721</identifier><identifier>EISSN: 2076-0299</identifier><identifier>DOI: 10.3329/bjms.v21i2.58063</identifier><language>eng</language><publisher>Dhaka: Ibn Sina Trust</publisher><subject>Antibiotics ; Ethanol ; Rhinitis ; Sinusitis</subject><ispartof>Bangladesh journal of medical science (Ibn Sina Trust), 2022-02, Vol.21 (2), p.311-317</ispartof><rights>2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-4d24b3404fcbea0e960088946b5a103d95f3c8ffec44d928bb73f9ae9c8832673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27923,27924</link.rule.ids></links><search><creatorcontrib>Widi Wardani, Andriana Tjitria</creatorcontrib><creatorcontrib>Purwanto, Bambang</creatorcontrib><creatorcontrib>DonoIndarto, -</creatorcontrib><creatorcontrib>Wasita, Brian</creatorcontrib><creatorcontrib>Cilmiaty AR, Risya</creatorcontrib><title>Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis</title><title>Bangladesh journal of medical science (Ibn Sina Trust)</title><description>Background: Symptoms and signs of acute viral and bacterial rhinosinusitis that are not specific cause difficulties in diagnostic enforcement, causing difficulties in administering therapy, so often doctors provide antibiotic therapy on acute viral rhinosinusitis. Irrelevant antibiotics will cause frequent antibiotic resistance. Adjuvant therapy is required to prevent infections that continue to become chronic, shorten the duration of infection, and avoid unwanted complications. Moringa leaves (M. oleifera)contains antioxidant components that can help rebalance redox metabolism and reduce excess free radicals so as to prevent oxidative stress. Method: posttest only control group research design. Male strain Sprague Dawley white rat was maintained and treated at the Nutrition Laboratory of the Center for Food and Nutrition Studies of Gadjah Mada University (UGM). Mice were divided into 4 groups. Group 1: standard feed., group 2: standard feed and staphylococcus-induced aureus, group 3: standard feed, Staphylococcus aureus-induced given amoxicillin dose of 27 mg/day, group 4: standard feed, Staphylococcus aureus-induced was given amoxicillin dose of 27 mg/day and given (EEDK) 200 mg/KgBW/day. Data analysis Of Normality test using Shapiro-Wilk Test and homogeneity using Levene’s Test obtained results p&gt;0.05. Then conducted One Way AnovaTest obtained p&lt;0.05 and continued with post-hoc LSD test. The data is analyzed using SPSS 25.0 for Windows. Result: MDA test Anova obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD all groups (p-value) &lt; 0.05, indicated a significant mean difference in average MDA levels among all groups. The lowest mean difference in MDA levels in group K with P3 was 0.92 pg/mL; while P1 with P3 has the largest average MDA rate difference of 7.47 pg/mL. TNF-α Anova test obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD of all groups (p-value= 0.000) &lt; 0.05, there is a meaningful difference in the average level of TNF-α between all groups. The smallest average difference in TNF-α levels in group K with P3 was 0.31 pg/mL; while P1 with P3 has the largest average TNF-α rate of 5.01 pg/mL. Description of inflammation of the mucosa cavum rice and sinus K score 0; P1 score 3; P2 score 2; P3 score 1. Conclusion: Moringa leaf ethanol extract in this study can be used as adjuvant therapy because it can lower MDA and TNF-α levels in mice induced with Staphylococcus aureus along with amoxicillin standard therapy. Extract ethanol moringa leaves in this study can be used as adjuvant therapy because it can lower the score of inflammatory cells better than by only given standard therapy only Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 311-317</description><subject>Antibiotics</subject><subject>Ethanol</subject><subject>Rhinitis</subject><subject>Sinusitis</subject><issn>2223-4721</issn><issn>2076-0299</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNotkUFOwzAQRSMEElXpnqUltqQ4tpPYy6q0UKkFqZR15Dg2dZXExXYqehrOwEU4E07LbGak_2a-Rj-KbhM4xhixh3LXuPEBJRqNUwozfBENEMyzGCLGLsOMEI5JjpLraOTcDobKIEwZHUTfM6Wk8MAosDJWtx8cLCVXYOa3vDU1mH15y3u9BWtZdUJWQT_I2p02Kn4PNi_z-PcH8LYCj9IJq_deBzrIi1bVvGm4N_YIprIOSzqc4R686bZzYNUJ43jwrWTd8xPReQnWW90a1wPaa3cTXSleOzn678PofT7bTJ_j5evTYjpZxgLliY9JhUiJCSRKlJJDycJ_lDKSlSlPIK5YqrCg_auEVAzRssyxYlwyQSlGWY6H0d357t6az046X-xMZ9tgWSCKsgTDlOBAwTMlrHHOSlXsrW64PRYJLPokij6J4pREcUoC_wHu8H6R</recordid><startdate>20220225</startdate><enddate>20220225</enddate><creator>Widi Wardani, Andriana Tjitria</creator><creator>Purwanto, Bambang</creator><creator>DonoIndarto, -</creator><creator>Wasita, Brian</creator><creator>Cilmiaty AR, Risya</creator><general>Ibn Sina Trust</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20220225</creationdate><title>Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis</title><author>Widi Wardani, Andriana Tjitria ; Purwanto, Bambang ; DonoIndarto, - ; Wasita, Brian ; Cilmiaty AR, Risya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-4d24b3404fcbea0e960088946b5a103d95f3c8ffec44d928bb73f9ae9c8832673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotics</topic><topic>Ethanol</topic><topic>Rhinitis</topic><topic>Sinusitis</topic><toplevel>online_resources</toplevel><creatorcontrib>Widi Wardani, Andriana Tjitria</creatorcontrib><creatorcontrib>Purwanto, Bambang</creatorcontrib><creatorcontrib>DonoIndarto, -</creatorcontrib><creatorcontrib>Wasita, Brian</creatorcontrib><creatorcontrib>Cilmiaty AR, Risya</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Bangladesh journal of medical science (Ibn Sina Trust)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Widi Wardani, Andriana Tjitria</au><au>Purwanto, Bambang</au><au>DonoIndarto, -</au><au>Wasita, Brian</au><au>Cilmiaty AR, Risya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis</atitle><jtitle>Bangladesh journal of medical science (Ibn Sina Trust)</jtitle><date>2022-02-25</date><risdate>2022</risdate><volume>21</volume><issue>2</issue><spage>311</spage><epage>317</epage><pages>311-317</pages><issn>2223-4721</issn><eissn>2076-0299</eissn><abstract>Background: Symptoms and signs of acute viral and bacterial rhinosinusitis that are not specific cause difficulties in diagnostic enforcement, causing difficulties in administering therapy, so often doctors provide antibiotic therapy on acute viral rhinosinusitis. Irrelevant antibiotics will cause frequent antibiotic resistance. Adjuvant therapy is required to prevent infections that continue to become chronic, shorten the duration of infection, and avoid unwanted complications. Moringa leaves (M. oleifera)contains antioxidant components that can help rebalance redox metabolism and reduce excess free radicals so as to prevent oxidative stress. Method: posttest only control group research design. Male strain Sprague Dawley white rat was maintained and treated at the Nutrition Laboratory of the Center for Food and Nutrition Studies of Gadjah Mada University (UGM). Mice were divided into 4 groups. Group 1: standard feed., group 2: standard feed and staphylococcus-induced aureus, group 3: standard feed, Staphylococcus aureus-induced given amoxicillin dose of 27 mg/day, group 4: standard feed, Staphylococcus aureus-induced was given amoxicillin dose of 27 mg/day and given (EEDK) 200 mg/KgBW/day. Data analysis Of Normality test using Shapiro-Wilk Test and homogeneity using Levene’s Test obtained results p&gt;0.05. Then conducted One Way AnovaTest obtained p&lt;0.05 and continued with post-hoc LSD test. The data is analyzed using SPSS 25.0 for Windows. Result: MDA test Anova obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD all groups (p-value) &lt; 0.05, indicated a significant mean difference in average MDA levels among all groups. The lowest mean difference in MDA levels in group K with P3 was 0.92 pg/mL; while P1 with P3 has the largest average MDA rate difference of 7.47 pg/mL. TNF-α Anova test obtained p value (Sig.) = 0.000 (p&lt; 0.05), Post Hoc with LSD of all groups (p-value= 0.000) &lt; 0.05, there is a meaningful difference in the average level of TNF-α between all groups. The smallest average difference in TNF-α levels in group K with P3 was 0.31 pg/mL; while P1 with P3 has the largest average TNF-α rate of 5.01 pg/mL. Description of inflammation of the mucosa cavum rice and sinus K score 0; P1 score 3; P2 score 2; P3 score 1. Conclusion: Moringa leaf ethanol extract in this study can be used as adjuvant therapy because it can lower MDA and TNF-α levels in mice induced with Staphylococcus aureus along with amoxicillin standard therapy. Extract ethanol moringa leaves in this study can be used as adjuvant therapy because it can lower the score of inflammatory cells better than by only given standard therapy only Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 311-317</abstract><cop>Dhaka</cop><pub>Ibn Sina Trust</pub><doi>10.3329/bjms.v21i2.58063</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2223-4721
ispartof Bangladesh journal of medical science (Ibn Sina Trust), 2022-02, Vol.21 (2), p.311-317
issn 2223-4721
2076-0299
language eng
recordid cdi_proquest_journals_2826130543
source DOAJ Directory of Open Access Journals
subjects Antibiotics
Ethanol
Rhinitis
Sinusitis
title Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T17%3A09%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Moringa%20Leaf%20Ethanol%20Extract%20on%20Reduced%20Levels%20of%20Mda,%20TNF-%CE%B1%20and%20Description%20of%20Inflammatory%20Cells%20in%20Rat%20Sinus%20Mucosa%20Model%20of%20Acute%20Rhinosinusitis&rft.jtitle=Bangladesh%20journal%20of%20medical%20science%20(Ibn%20Sina%20Trust)&rft.au=Widi%20Wardani,%20Andriana%20Tjitria&rft.date=2022-02-25&rft.volume=21&rft.issue=2&rft.spage=311&rft.epage=317&rft.pages=311-317&rft.issn=2223-4721&rft.eissn=2076-0299&rft_id=info:doi/10.3329/bjms.v21i2.58063&rft_dat=%3Cproquest_cross%3E2826130543%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2826130543&rft_id=info:pmid/&rfr_iscdi=true