Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245
— Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123:: aphAI mutant of Azospirillum baldaniorum Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O po...
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| Veröffentlicht in: | Microbiology (New York) 2023-06, Vol.92 (3), p.389-397 |
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| creator | Petrova, L. P. Evstigneeva, S. S. Filip’echeva, Y. A. Volokhina, I. V. Burygin, G. L. Matora, L. Y. Shelud’ko, A. V. |
| description | —
Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::
aphAI
mutant of
Azospirillum baldaniorum
Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::
aphAI
mutant Sp245.4-1-1 accumulated two times less biomass than did those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains. |
| doi_str_mv | 10.1134/S0026261723600283 |
| format | Article |
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Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::
aphAI
mutant of
Azospirillum baldaniorum
Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::
aphAI
mutant Sp245.4-1-1 accumulated two times less biomass than did those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains.</description><identifier>ISSN: 0026-2617</identifier><identifier>EISSN: 1608-3237</identifier><identifier>DOI: 10.1134/S0026261723600283</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>ABC transporter ; ATP-binding protein ; Azospirillum ; Biofilms ; Biomass ; Biomedical and Life Sciences ; Experimental Articles ; Life Sciences ; Lipopolysaccharides ; Medical Microbiology ; Microbiology ; Mutants ; Polysaccharides ; Protein biosynthesis ; Protein transport</subject><ispartof>Microbiology (New York), 2023-06, Vol.92 (3), p.389-397</ispartof><rights>Pleiades Publishing, Ltd. 2023. ISSN 0026-2617, Microbiology, 2023, Vol. 92, No. 3, pp. 389–397. © Pleiades Publishing, Ltd., 2023. Russian Text © The Author(s), 2023, published in Mikrobiologiya, 2023, Vol. 92, No. 3, pp. 289–299.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-37c2a97d16a93b7e8975e9e76a9c4182c9901f6dbf29fdce1a003def164616f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026261723600283$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026261723600283$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Petrova, L. P.</creatorcontrib><creatorcontrib>Evstigneeva, S. S.</creatorcontrib><creatorcontrib>Filip’echeva, Y. A.</creatorcontrib><creatorcontrib>Volokhina, I. V.</creatorcontrib><creatorcontrib>Burygin, G. L.</creatorcontrib><creatorcontrib>Matora, L. Y.</creatorcontrib><creatorcontrib>Shelud’ko, A. V.</creatorcontrib><title>Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245</title><title>Microbiology (New York)</title><addtitle>Microbiology</addtitle><description>—
Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::
aphAI
mutant of
Azospirillum baldaniorum
Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::
aphAI
mutant Sp245.4-1-1 accumulated two times less biomass than did those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains.</description><subject>ABC transporter</subject><subject>ATP-binding protein</subject><subject>Azospirillum</subject><subject>Biofilms</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Experimental Articles</subject><subject>Life Sciences</subject><subject>Lipopolysaccharides</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mutants</subject><subject>Polysaccharides</subject><subject>Protein biosynthesis</subject><subject>Protein transport</subject><issn>0026-2617</issn><issn>1608-3237</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kctKAzEUhoMoWKsP4C7gejSXTjKzbKVWodBiFcHNkOaiKTPJmEwX7QP5nGas4EJc5RzO9_0HcgC4xOgaYzq6WSFEGGGYE8pSWdAjMMAMFRkllB-DQT_O-vkpOItxgxDKSZ4PwOfUGC076A3s3jUcvy4mj1XLECYULmsRG6vgTDsNp056Zd3bN_ay7-Ay-E5bB72Dc9v61te7KKR8F8EqDVc7l8BoIxROwYn1xtYNvPOhEZ1NShL7oImQnQ5228Dx3sfWBlvXqVmLWglnfUj1qiWj_BycGFFHffHzDsHz3fTp9j6bL2YPt-N5JgkruoxySUTJFWaipGuui5LnutQ8tXKECyLLEmHD1NqQ0iipsUCIKm0wGzHMTE6H4OqQ2wb_sdWxqzZ-G1xaWZGCYMbTN_JE4QMlg48xaFO1wTYi7CqMqv4c1Z9zJIccnJhY96bDb_L_0hfS84zY</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Petrova, L. P.</creator><creator>Evstigneeva, S. S.</creator><creator>Filip’echeva, Y. A.</creator><creator>Volokhina, I. V.</creator><creator>Burygin, G. L.</creator><creator>Matora, L. Y.</creator><creator>Shelud’ko, A. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20230601</creationdate><title>Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245</title><author>Petrova, L. P. ; Evstigneeva, S. S. ; Filip’echeva, Y. A. ; Volokhina, I. V. ; Burygin, G. L. ; Matora, L. Y. ; Shelud’ko, A. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-37c2a97d16a93b7e8975e9e76a9c4182c9901f6dbf29fdce1a003def164616f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>ABC transporter</topic><topic>ATP-binding protein</topic><topic>Azospirillum</topic><topic>Biofilms</topic><topic>Biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Experimental Articles</topic><topic>Life Sciences</topic><topic>Lipopolysaccharides</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Mutants</topic><topic>Polysaccharides</topic><topic>Protein biosynthesis</topic><topic>Protein transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petrova, L. P.</creatorcontrib><creatorcontrib>Evstigneeva, S. S.</creatorcontrib><creatorcontrib>Filip’echeva, Y. A.</creatorcontrib><creatorcontrib>Volokhina, I. V.</creatorcontrib><creatorcontrib>Burygin, G. L.</creatorcontrib><creatorcontrib>Matora, L. Y.</creatorcontrib><creatorcontrib>Shelud’ko, A. V.</creatorcontrib><collection>CrossRef</collection><jtitle>Microbiology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petrova, L. P.</au><au>Evstigneeva, S. S.</au><au>Filip’echeva, Y. A.</au><au>Volokhina, I. V.</au><au>Burygin, G. L.</au><au>Matora, L. Y.</au><au>Shelud’ko, A. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245</atitle><jtitle>Microbiology (New York)</jtitle><stitle>Microbiology</stitle><date>2023-06-01</date><risdate>2023</risdate><volume>92</volume><issue>3</issue><spage>389</spage><epage>397</epage><pages>389-397</pages><issn>0026-2617</issn><eissn>1608-3237</eissn><abstract>—
Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::
aphAI
mutant of
Azospirillum baldaniorum
Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::
aphAI
mutant Sp245.4-1-1 accumulated two times less biomass than did those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0026261723600283</doi><tpages>9</tpages></addata></record> |
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| subjects | ABC transporter ATP-binding protein Azospirillum Biofilms Biomass Biomedical and Life Sciences Experimental Articles Life Sciences Lipopolysaccharides Medical Microbiology Microbiology Mutants Polysaccharides Protein biosynthesis Protein transport |
| title | Effect of the AZOBR_p60123 Plasmid Gene Encoding the Wzt Protein on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245 |
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