Induction of shoot and root on planlet using synthetic hormones of BAP and NAA during in vitro multiplication of Chrysanthemum sp. Puma white and Kineta variety

Chrysanthemum is an annual plant with a high commercial value and the greatest development potential. Along with propagation, demand for chrysanthemum plants is inversely proportional to the supply of high-quality parent plants. Thus, there is a need for maximum sustainable production by preserving chrysanthemum plants. Still, conventional multiplication has numerous limitations, necessitating tissue culture techniques to overcome the scarcity of chrysanthemum seeds. This study aimed to determine the effect of shoot multiplication and root induction on plantlets of Chrysanthemum sp—Puma white and Kineta types when growth regulators of BAP and NAA were added in-vitro. The research is quantitative, demonstrating the link between variables. The research approach is experimental, with nine treatments: MS, BAP 2 ml/L, BAP 4 ml/L, NAA 2 ml/L, NAA 4 ml/L, BAP 2 ml/L+NAA 2 ml/L + BAP 2 ml/L+NAA 4 ml/L, BAP 4 + NAA 2 ml/L,and BAP 4 + NAA 4 ml/L. The study results on two Chrysanthemum types, Puma white, and Kineta, indicate that NAA 2 mg/L significantly influences plant height. BAP 2 mg/L has a significant effect on leaf number shoot induction. NAA 4 mg/L has a significant effect on root induction.