Optimization of Biolistic Transformation Parameters for Nicotiana tabacum
Biolistics is one of the widely used methods to deliver nucleic acids into plant cells. In this work, we optimized the protocol for biolistic transformation of Nicotiana tabacum with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a m...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2023-06, Vol.59 (3), p.368-372 |
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| container_title | Applied biochemistry and microbiology |
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| creator | Davlekamova, A. A. Zubritsky, A. V. Timofeeva, T. A. Yakovleva, I. V. Kamionskaya, A. M. |
| description | Biolistics is one of the widely used methods to deliver nucleic acids into plant cells. In this work, we optimized the protocol for biolistic transformation of
Nicotiana tabacum
with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a marker. The optimal parameters for the transformation of
N. tabacum
leaf cells were determined as: pressure, 1350 psi; tungsten particle size, 1.3 μm; plasmid DNA purification method, ethanol re-precipitation. The results should be useful for the development of biolistic transformation protocols for plant cells, including application for plant genome editing of agricultural species. |
| doi_str_mv | 10.1134/S0003683823030055 |
| format | Article |
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Nicotiana tabacum
with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a marker. The optimal parameters for the transformation of
N. tabacum
leaf cells were determined as: pressure, 1350 psi; tungsten particle size, 1.3 μm; plasmid DNA purification method, ethanol re-precipitation. The results should be useful for the development of biolistic transformation protocols for plant cells, including application for plant genome editing of agricultural species.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683823030055</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Ethanol ; Fluorescence ; Gene guns ; Genetic transformation ; Genome editing ; Genomes ; Green fluorescent protein ; Life Sciences ; Medical Microbiology ; Microbiology ; Nicotiana tabacum ; Nucleic acids ; Optimization ; Parameters ; Plant cells ; Plasmids ; Transformations ; Tungsten</subject><ispartof>Applied biochemistry and microbiology, 2023-06, Vol.59 (3), p.368-372</ispartof><rights>Pleiades Publishing, Inc. 2023. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2023, Vol. 59, No. 3, pp. 368–372. © Pleiades Publishing, Inc., 2023. Russian Text © The Author(s), 2023, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2023, Vol. 59, No. 3, pp. 289–294.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-36b07c248dbd901cdf3fd9c63e86c51b0865ae68429dd6202cd926e3f1b1e513</citedby><cites>FETCH-LOGICAL-c316t-36b07c248dbd901cdf3fd9c63e86c51b0865ae68429dd6202cd926e3f1b1e513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683823030055$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683823030055$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Davlekamova, A. A.</creatorcontrib><creatorcontrib>Zubritsky, A. V.</creatorcontrib><creatorcontrib>Timofeeva, T. A.</creatorcontrib><creatorcontrib>Yakovleva, I. V.</creatorcontrib><creatorcontrib>Kamionskaya, A. M.</creatorcontrib><title>Optimization of Biolistic Transformation Parameters for Nicotiana tabacum</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Biolistics is one of the widely used methods to deliver nucleic acids into plant cells. In this work, we optimized the protocol for biolistic transformation of
Nicotiana tabacum
with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a marker. The optimal parameters for the transformation of
N. tabacum
leaf cells were determined as: pressure, 1350 psi; tungsten particle size, 1.3 μm; plasmid DNA purification method, ethanol re-precipitation. The results should be useful for the development of biolistic transformation protocols for plant cells, including application for plant genome editing of agricultural species.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Ethanol</subject><subject>Fluorescence</subject><subject>Gene guns</subject><subject>Genetic transformation</subject><subject>Genome editing</subject><subject>Genomes</subject><subject>Green fluorescent protein</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Nicotiana tabacum</subject><subject>Nucleic acids</subject><subject>Optimization</subject><subject>Parameters</subject><subject>Plant cells</subject><subject>Plasmids</subject><subject>Transformations</subject><subject>Tungsten</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWKsfwNuC59WZZDdNjlq0FooV7H3J5o-kdDc1SQ_66d2yggfxNMz83nsDj5BrhFtEVt29AQDjggnKgAHU9QmZIAdRMqDVKZkccXnk5-Qipe2wSi7khCzX--w7_6WyD30RXPHgw86n7HWxiapPLsRuZK8qqs5mG1MxHIsXr0P2qldFVq3Sh-6SnDm1S_bqZ07J5ulxM38uV-vFcn6_KjVDnkvGW5hpWgnTGgmojWPOSM2ZFVzX2ILgtbJcVFQawylQbSTlljls0dbIpuRmjN3H8HGwKTfbcIj98LGhAmWFs2pGBxWOKh1DStG6Zh99p-Jng9AcC2v-FDZ46OhJg7Z_t_E3-X_TN08FbLo</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Davlekamova, A. A.</creator><creator>Zubritsky, A. V.</creator><creator>Timofeeva, T. A.</creator><creator>Yakovleva, I. V.</creator><creator>Kamionskaya, A. M.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20230601</creationdate><title>Optimization of Biolistic Transformation Parameters for Nicotiana tabacum</title><author>Davlekamova, A. A. ; Zubritsky, A. V. ; Timofeeva, T. A. ; Yakovleva, I. V. ; Kamionskaya, A. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-36b07c248dbd901cdf3fd9c63e86c51b0865ae68429dd6202cd926e3f1b1e513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Ethanol</topic><topic>Fluorescence</topic><topic>Gene guns</topic><topic>Genetic transformation</topic><topic>Genome editing</topic><topic>Genomes</topic><topic>Green fluorescent protein</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Nicotiana tabacum</topic><topic>Nucleic acids</topic><topic>Optimization</topic><topic>Parameters</topic><topic>Plant cells</topic><topic>Plasmids</topic><topic>Transformations</topic><topic>Tungsten</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davlekamova, A. A.</creatorcontrib><creatorcontrib>Zubritsky, A. V.</creatorcontrib><creatorcontrib>Timofeeva, T. A.</creatorcontrib><creatorcontrib>Yakovleva, I. V.</creatorcontrib><creatorcontrib>Kamionskaya, A. M.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davlekamova, A. A.</au><au>Zubritsky, A. V.</au><au>Timofeeva, T. A.</au><au>Yakovleva, I. V.</au><au>Kamionskaya, A. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of Biolistic Transformation Parameters for Nicotiana tabacum</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2023-06-01</date><risdate>2023</risdate><volume>59</volume><issue>3</issue><spage>368</spage><epage>372</epage><pages>368-372</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Biolistics is one of the widely used methods to deliver nucleic acids into plant cells. In this work, we optimized the protocol for biolistic transformation of
Nicotiana tabacum
with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a marker. The optimal parameters for the transformation of
N. tabacum
leaf cells were determined as: pressure, 1350 psi; tungsten particle size, 1.3 μm; plasmid DNA purification method, ethanol re-precipitation. The results should be useful for the development of biolistic transformation protocols for plant cells, including application for plant genome editing of agricultural species.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683823030055</doi><tpages>5</tpages></addata></record> |
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| ispartof | Applied biochemistry and microbiology, 2023-06, Vol.59 (3), p.368-372 |
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| language | eng |
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| source | SpringerLink (Online service) |
| subjects | Biochemistry Biomedical and Life Sciences Ethanol Fluorescence Gene guns Genetic transformation Genome editing Genomes Green fluorescent protein Life Sciences Medical Microbiology Microbiology Nicotiana tabacum Nucleic acids Optimization Parameters Plant cells Plasmids Transformations Tungsten |
| title | Optimization of Biolistic Transformation Parameters for Nicotiana tabacum |
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