In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality
An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use of different PGRs, basal media and ethylene adsorbents. Incorporation of cytokinins, BAP (8.8 µM) and kinetin (6.97 µM) resulted in best shoot organogenesis with the highest response (8...
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| description | An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use of different PGRs, basal media and ethylene adsorbents. Incorporation of cytokinins, BAP (8.8 µM) and kinetin (6.97 µM) resulted in best shoot organogenesis with the highest response (81.40%), no. of micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) and no. of leaves/micro-shoot (3.55) owing to complementary effect of these factors. However, the regenerated micro-shoots failed to establish due to 100% leaf abscission of micro-shoot. To retard the effect of ethylene accumulation on the regenerated micro-shoots, two types of ethylene adsorbents carrying silver ions, namely, AgNO
3
and Ag
2
S
2
O
3
and two gelling agents (agar–agar and Phytagel™) were tested at different concentrations. Addition of AgNO
3
(5.88 µM) to the medium containing Phytagel™ along with cytokinin (BAP 8.8 µM and kinetin 6.97 µM) led to significant reduction in shoot abscission rate (4.20), while 17.66 µM AgNO
3
supplementation improved no. of micro-shoots/explants (2.19) and micro-shoot length (3.36 cm) whereas Ag
2
S
2
O
3
at 20 µM enhanced the total chlorophyll content (3.47 mg g
−1
FW) three times as compared to control. Similarly, among the tested basal media, MS basal medium induced best response on shoot organogenesis. Rooting of micro-shoots was highest (81.12%) with the supplementation of NAA (5.37 µM), which also affected no. of roots/explant (4.52) and mean root length (5.26 cm). The supplementation of ethylene adsorbents during in vitro micro-shoot multiplication significantly improved their quality, which provides ideal rooting for development of complete plantlets in sweet orange cv. Mosambi.
Key message
Ethylene accumulation in glass containers inhibited micro-shoot establishment of
Citrus sinensis
L. Use of silver ions enhanced culture establishment and quality of the micro-shoot. |
| doi_str_mv | 10.1007/s11240-023-02499-2 |
| format | Article |
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3
and Ag
2
S
2
O
3
and two gelling agents (agar–agar and Phytagel™) were tested at different concentrations. Addition of AgNO
3
(5.88 µM) to the medium containing Phytagel™ along with cytokinin (BAP 8.8 µM and kinetin 6.97 µM) led to significant reduction in shoot abscission rate (4.20), while 17.66 µM AgNO
3
supplementation improved no. of micro-shoots/explants (2.19) and micro-shoot length (3.36 cm) whereas Ag
2
S
2
O
3
at 20 µM enhanced the total chlorophyll content (3.47 mg g
−1
FW) three times as compared to control. Similarly, among the tested basal media, MS basal medium induced best response on shoot organogenesis. Rooting of micro-shoots was highest (81.12%) with the supplementation of NAA (5.37 µM), which also affected no. of roots/explant (4.52) and mean root length (5.26 cm). The supplementation of ethylene adsorbents during in vitro micro-shoot multiplication significantly improved their quality, which provides ideal rooting for development of complete plantlets in sweet orange cv. Mosambi.
Key message
Ethylene accumulation in glass containers inhibited micro-shoot establishment of
Citrus sinensis
L. Use of silver ions enhanced culture establishment and quality of the micro-shoot.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02499-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abscission ; Accumulation ; Adsorbents ; Agar ; Biomedical and Life Sciences ; Chlorophyll ; Citrus fruits ; Citrus sinensis ; Cytokinins ; Ethylene ; Explants ; Fruits ; Ions ; Kinetin ; Leaves ; Life Sciences ; Multiplication ; Oranges ; Organogenesis ; Original Article ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plantlets ; Reagents ; Rooting ; Shoots ; Silver nitrate</subject><ispartof>Plant cell, tissue and organ culture, 2023-06, Vol.153 (3), p.625-637</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-c91070fee44428d9968f01a114b8c1171527912dec407bef075e307f48f041ec3</citedby><cites>FETCH-LOGICAL-c363t-c91070fee44428d9968f01a114b8c1171527912dec407bef075e307f48f041ec3</cites><orcidid>0000-0002-9222-8605</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02499-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02499-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Prusty, Reena</creatorcontrib><creatorcontrib>Awasthi, O. P.</creatorcontrib><creatorcontrib>Singh, S. K.</creatorcontrib><creatorcontrib>Kanika Kumar</creatorcontrib><title>In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use of different PGRs, basal media and ethylene adsorbents. Incorporation of cytokinins, BAP (8.8 µM) and kinetin (6.97 µM) resulted in best shoot organogenesis with the highest response (81.40%), no. of micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) and no. of leaves/micro-shoot (3.55) owing to complementary effect of these factors. However, the regenerated micro-shoots failed to establish due to 100% leaf abscission of micro-shoot. To retard the effect of ethylene accumulation on the regenerated micro-shoots, two types of ethylene adsorbents carrying silver ions, namely, AgNO
3
and Ag
2
S
2
O
3
and two gelling agents (agar–agar and Phytagel™) were tested at different concentrations. Addition of AgNO
3
(5.88 µM) to the medium containing Phytagel™ along with cytokinin (BAP 8.8 µM and kinetin 6.97 µM) led to significant reduction in shoot abscission rate (4.20), while 17.66 µM AgNO
3
supplementation improved no. of micro-shoots/explants (2.19) and micro-shoot length (3.36 cm) whereas Ag
2
S
2
O
3
at 20 µM enhanced the total chlorophyll content (3.47 mg g
−1
FW) three times as compared to control. Similarly, among the tested basal media, MS basal medium induced best response on shoot organogenesis. Rooting of micro-shoots was highest (81.12%) with the supplementation of NAA (5.37 µM), which also affected no. of roots/explant (4.52) and mean root length (5.26 cm). The supplementation of ethylene adsorbents during in vitro micro-shoot multiplication significantly improved their quality, which provides ideal rooting for development of complete plantlets in sweet orange cv. Mosambi.
Key message
Ethylene accumulation in glass containers inhibited micro-shoot establishment of
Citrus sinensis
L. Use of silver ions enhanced culture establishment and quality of the micro-shoot.</description><subject>Abscission</subject><subject>Accumulation</subject><subject>Adsorbents</subject><subject>Agar</subject><subject>Biomedical and Life Sciences</subject><subject>Chlorophyll</subject><subject>Citrus fruits</subject><subject>Citrus sinensis</subject><subject>Cytokinins</subject><subject>Ethylene</subject><subject>Explants</subject><subject>Fruits</subject><subject>Ions</subject><subject>Kinetin</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Multiplication</subject><subject>Oranges</subject><subject>Organogenesis</subject><subject>Original Article</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Reagents</subject><subject>Rooting</subject><subject>Shoots</subject><subject>Silver nitrate</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMFqGzEQhkVJoE7SF-hJ0Et7WGdG0q52j8WkacAll_Qs5PXI3mBLiWad4FfIU1fOFnrrYRAM3_-P-IT4jDBHAHvNiMpABUqXMV1XqQ9ihrXVVQ3GnIkZYGOrpq3tR3HB_AgAjTY4E293Ub4MY06StymNMuWNj2lDkXhgOUTJr0SntY8bkl8XBT2w5CFSPAHL-TfZv8zlr8R-vxqkj2s5bklSCNSXWJA0bo-7Uif9mlNeURxZpij3Q59TNd18PvjdMB6vxHnwO6ZPf99L8fvHzcPiZ7W8v71bfF9WvW70WPUdgoVAZIxR7brrmjYAekSzantEi7WyHao19QbsigLYmjTYYApmkHp9Kb5MvU85PR-IR_eYDjmWk061qG1TN9AVSk1U-SdzpuCe8rD3-egQ3Mm5m5y74ty9O3eqhPQU4gIXYflf9X9SfwCeu4UC</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Prusty, Reena</creator><creator>Awasthi, O. P.</creator><creator>Singh, S. K.</creator><creator>Kanika Kumar</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0002-9222-8605</orcidid></search><sort><creationdate>20230601</creationdate><title>In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality</title><author>Prusty, Reena ; Awasthi, O. P. ; Singh, S. K. ; Kanika Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-c91070fee44428d9968f01a114b8c1171527912dec407bef075e307f48f041ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abscission</topic><topic>Accumulation</topic><topic>Adsorbents</topic><topic>Agar</topic><topic>Biomedical and Life Sciences</topic><topic>Chlorophyll</topic><topic>Citrus fruits</topic><topic>Citrus sinensis</topic><topic>Cytokinins</topic><topic>Ethylene</topic><topic>Explants</topic><topic>Fruits</topic><topic>Ions</topic><topic>Kinetin</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Multiplication</topic><topic>Oranges</topic><topic>Organogenesis</topic><topic>Original Article</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Reagents</topic><topic>Rooting</topic><topic>Shoots</topic><topic>Silver nitrate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prusty, Reena</creatorcontrib><creatorcontrib>Awasthi, O. P.</creatorcontrib><creatorcontrib>Singh, S. 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P.</au><au>Singh, S. K.</au><au>Kanika Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2023-06-01</date><risdate>2023</risdate><volume>153</volume><issue>3</issue><spage>625</spage><epage>637</epage><pages>625-637</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use of different PGRs, basal media and ethylene adsorbents. Incorporation of cytokinins, BAP (8.8 µM) and kinetin (6.97 µM) resulted in best shoot organogenesis with the highest response (81.40%), no. of micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) and no. of leaves/micro-shoot (3.55) owing to complementary effect of these factors. However, the regenerated micro-shoots failed to establish due to 100% leaf abscission of micro-shoot. To retard the effect of ethylene accumulation on the regenerated micro-shoots, two types of ethylene adsorbents carrying silver ions, namely, AgNO
3
and Ag
2
S
2
O
3
and two gelling agents (agar–agar and Phytagel™) were tested at different concentrations. Addition of AgNO
3
(5.88 µM) to the medium containing Phytagel™ along with cytokinin (BAP 8.8 µM and kinetin 6.97 µM) led to significant reduction in shoot abscission rate (4.20), while 17.66 µM AgNO
3
supplementation improved no. of micro-shoots/explants (2.19) and micro-shoot length (3.36 cm) whereas Ag
2
S
2
O
3
at 20 µM enhanced the total chlorophyll content (3.47 mg g
−1
FW) three times as compared to control. Similarly, among the tested basal media, MS basal medium induced best response on shoot organogenesis. Rooting of micro-shoots was highest (81.12%) with the supplementation of NAA (5.37 µM), which also affected no. of roots/explant (4.52) and mean root length (5.26 cm). The supplementation of ethylene adsorbents during in vitro micro-shoot multiplication significantly improved their quality, which provides ideal rooting for development of complete plantlets in sweet orange cv. Mosambi.
Key message
Ethylene accumulation in glass containers inhibited micro-shoot establishment of
Citrus sinensis
L. Use of silver ions enhanced culture establishment and quality of the micro-shoot.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02499-2</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9222-8605</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2023-06, Vol.153 (3), p.625-637 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_journals_2813765609 |
| source | SpringerNature Journals |
| subjects | Abscission Accumulation Adsorbents Agar Biomedical and Life Sciences Chlorophyll Citrus fruits Citrus sinensis Cytokinins Ethylene Explants Fruits Ions Kinetin Leaves Life Sciences Multiplication Oranges Organogenesis Original Article Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plantlets Reagents Rooting Shoots Silver nitrate |
| title | In vitro shoot organogenesis in sweet orange (Citrus sinensis L.) cv. Mosambi and the effect of ethylene adsorbents on micro-shoot quality |
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