DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori
The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bombyx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the polyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygote...
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| Veröffentlicht in: | Journal of Insect Biotechnology and Sericology 2022, Vol.91(3), pp.3_041-3_050 |
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| container_title | Journal of Insect Biotechnology and Sericology |
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| creator | Fujii, Tsuguru Kubo, Maki Kuwazaki, Seigou Yamamoto, Kimiko Ohnuma, Akio Banno, Yutaka Shimada, Toru |
| description | The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bombyx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the polyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, morphogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypothesized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed. |
| doi_str_mv | 10.11416/jibs.91.3_041 |
| format | Article |
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Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the polyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, morphogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypothesized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed.</description><identifier>ISSN: 1346-8073</identifier><identifier>EISSN: 1884-7978</identifier><identifier>DOI: 10.11416/jibs.91.3_041</identifier><language>eng</language><publisher>Ibaraki: The Japanese Society of Sericultural Science</publisher><subject>Bombyx mori ; chromosomal aberration ; Chromosome 11 ; Chromosome aberrations ; Chromosomes ; deletion ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA sequencing ; Embryogenesis ; Embryonic growth stage ; Feeding behavior ; Gene sequencing ; Heterozygotes ; Homozygotes ; Irradiation ; Larvae ; lethal mutant ; Lethality ; Morphogenesis ; Mutation ; Nucleotide sequence ; Phenotypes ; polyphagous mutant ; Radiation ; Radiation effects ; Sequence analysis ; Trisomy</subject><ispartof>Journal of Insect Biotechnology and Sericology, 2022, Vol.91(3), pp.3_041-3_050</ispartof><rights>2022 by Japan Academic Association for Copyright Clearance (Except in the USA), Copyright Clearance Center, Inc. (In the USA)</rights><rights>Copyright Japan Science and Technology Agency 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Fujii, Tsuguru</creatorcontrib><creatorcontrib>Kubo, Maki</creatorcontrib><creatorcontrib>Kuwazaki, Seigou</creatorcontrib><creatorcontrib>Yamamoto, Kimiko</creatorcontrib><creatorcontrib>Ohnuma, Akio</creatorcontrib><creatorcontrib>Banno, Yutaka</creatorcontrib><creatorcontrib>Shimada, Toru</creatorcontrib><title>DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori</title><title>Journal of Insect Biotechnology and Sericology</title><addtitle>J. Insect Biotechnol. Sericol.</addtitle><description>The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bombyx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the polyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, morphogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypothesized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed.</description><subject>Bombyx mori</subject><subject>chromosomal aberration</subject><subject>Chromosome 11</subject><subject>Chromosome aberrations</subject><subject>Chromosomes</subject><subject>deletion</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA sequencing</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Feeding behavior</subject><subject>Gene sequencing</subject><subject>Heterozygotes</subject><subject>Homozygotes</subject><subject>Irradiation</subject><subject>Larvae</subject><subject>lethal mutant</subject><subject>Lethality</subject><subject>Morphogenesis</subject><subject>Mutation</subject><subject>Nucleotide sequence</subject><subject>Phenotypes</subject><subject>polyphagous mutant</subject><subject>Radiation</subject><subject>Radiation effects</subject><subject>Sequence analysis</subject><subject>Trisomy</subject><issn>1346-8073</issn><issn>1884-7978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNo9UE1PwzAMjRBIjMGVcyQucOhImqRJbmzjU5rgAufIbTOWqm1G0krbvydogGTZz_aTPx5Cl5TMKOW0uG1cGWeazpghnB6hCVWKZ1JLdZww40WmiGSn6CzGhhDGhOAT5O5f5zjar9H2lcXQQ7uPLmK_xoCrTfCdj76DFkNpQ4DB-R67ZAnX7i-tx8rWuBxDP-DrxXiDu3GAhNOQhe_K_Q53PrhzdLKGNtqL3zhFH48P78vnbPX29LKcr7KGETFkoiK65IWuwbKCsYpqKYikPNdsDTkQZYWkxPJSWJULIWxd0lpCncpMW6nYFF0d5m6DT2_FwTQ-nZZWmlwlGZQmBU-suwOriQN8WrMNroOwNxAGV7XW_GhpNDXs4JKg_61qA8HYnn0D1OlxJw</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Fujii, Tsuguru</creator><creator>Kubo, Maki</creator><creator>Kuwazaki, Seigou</creator><creator>Yamamoto, Kimiko</creator><creator>Ohnuma, Akio</creator><creator>Banno, Yutaka</creator><creator>Shimada, Toru</creator><general>The Japanese Society of Sericultural Science</general><general>Japan Science and Technology Agency</general><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2022</creationdate><title>DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori</title><author>Fujii, Tsuguru ; Kubo, Maki ; Kuwazaki, Seigou ; Yamamoto, Kimiko ; Ohnuma, Akio ; Banno, Yutaka ; Shimada, Toru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j305t-5c09b469dae3633c19750714293fa2a08e5710e4b5e82555edb1d7ade5739e783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bombyx mori</topic><topic>chromosomal aberration</topic><topic>Chromosome 11</topic><topic>Chromosome aberrations</topic><topic>Chromosomes</topic><topic>deletion</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA sequencing</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Feeding behavior</topic><topic>Gene sequencing</topic><topic>Heterozygotes</topic><topic>Homozygotes</topic><topic>Irradiation</topic><topic>Larvae</topic><topic>lethal mutant</topic><topic>Lethality</topic><topic>Morphogenesis</topic><topic>Mutation</topic><topic>Nucleotide sequence</topic><topic>Phenotypes</topic><topic>polyphagous mutant</topic><topic>Radiation</topic><topic>Radiation effects</topic><topic>Sequence analysis</topic><topic>Trisomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujii, Tsuguru</creatorcontrib><creatorcontrib>Kubo, Maki</creatorcontrib><creatorcontrib>Kuwazaki, Seigou</creatorcontrib><creatorcontrib>Yamamoto, Kimiko</creatorcontrib><creatorcontrib>Ohnuma, Akio</creatorcontrib><creatorcontrib>Banno, Yutaka</creatorcontrib><creatorcontrib>Shimada, Toru</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of Insect Biotechnology and Sericology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujii, Tsuguru</au><au>Kubo, Maki</au><au>Kuwazaki, Seigou</au><au>Yamamoto, Kimiko</au><au>Ohnuma, Akio</au><au>Banno, Yutaka</au><au>Shimada, Toru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori</atitle><jtitle>Journal of Insect Biotechnology and Sericology</jtitle><addtitle>J. Insect Biotechnol. Sericol.</addtitle><date>2022</date><risdate>2022</risdate><volume>91</volume><issue>3</issue><spage>3_041</spage><epage>3_050</epage><pages>3_041-3_050</pages><issn>1346-8073</issn><eissn>1884-7978</eissn><abstract>The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bombyx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the polyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, morphogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypothesized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed.</abstract><cop>Ibaraki</cop><pub>The Japanese Society of Sericultural Science</pub><doi>10.11416/jibs.91.3_041</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Bombyx mori chromosomal aberration Chromosome 11 Chromosome aberrations Chromosomes deletion Deoxyribonucleic acid DNA DNA damage DNA sequencing Embryogenesis Embryonic growth stage Feeding behavior Gene sequencing Heterozygotes Homozygotes Irradiation Larvae lethal mutant Lethality Morphogenesis Mutation Nucleotide sequence Phenotypes polyphagous mutant Radiation Radiation effects Sequence analysis Trisomy |
| title | DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori |
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