Genetic stability of cryopreserved ornamental Lilium germplasm

The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated pla...

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Veröffentlicht in:Plant genetic resources: characterization and utilization 2022-02, Vol.20 (1), p.66-68
Hauptverfasser: Song, Jae-young, Yi, Jung-yoon, Bae, Jinjoo, Lee, Jung-ro, Yoon, Mun-sup, Lee, Young-yi
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container_end_page 68
container_issue 1
container_start_page 66
container_title Plant genetic resources: characterization and utilization
container_volume 20
creator Song, Jae-young
Yi, Jung-yoon
Bae, Jinjoo
Lee, Jung-ro
Yoon, Mun-sup
Lee, Young-yi
description The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars ( L . bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety ( L. callosum var. flavum ) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. The results suggest a very low level or the absence of genetic variation in terms of morphological and genetic stability among the plants regenerated after cryopreservation.
doi_str_mv 10.1017/S147926212200003X
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As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars ( L . bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety ( L. callosum var. flavum ) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. 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source CUP_剑桥大学出版社现刊
subjects Cryopreservation
Cultivars
Genetic diversity
Germplasm
Lilium
Lilium bolanderi
Low level
Morphology
Plantlets
Plants (botany)
Pollen
Stability
Stigmas (botany)
title Genetic stability of cryopreserved ornamental Lilium germplasm
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