Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)
The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or...
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| Veröffentlicht in: | Annals of animal science 2023-04, Vol.23 (2), p.461-471 |
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| creator | Sherif, Rasha M. Talat, Dalia Alaidaroos, Bothaina A. Farsi, Reem M. Hassoubah, Shahira A. Jaber, Fatima A. Azer, Treza M. El-Masry, Reham M. Abd El-Hack, Mohamed E. Ibrahim, Madiha S. Elbestawy, Ahmed |
| description | The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing
is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against
O26 and its Shiga toxin type 2 (
). Multidrug resistance phenotype was observed in
O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of
O26 (10
, 10
and 10
CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in
level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the
gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in
O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on
O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against
O26. |
| doi_str_mv | 10.2478/aoas-2022-0088 |
| format | Article |
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is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against
O26 and its Shiga toxin type 2 (
). Multidrug resistance phenotype was observed in
O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of
O26 (10
, 10
and 10
CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in
level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the
gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in
O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on
O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against
O26.</description><identifier>ISSN: 2300-8733</identifier><identifier>ISSN: 1642-3402</identifier><identifier>EISSN: 2300-8733</identifier><identifier>DOI: 10.2478/aoas-2022-0088</identifier><language>eng</language><publisher>Kraków: Sciendo</publisher><subject>Antibacterial activity ; Antibacterial agents ; Antimicrobial activity ; Antimicrobial agents ; Bacteria ; Cell density ; Colitis ; E coli ; Electron micrographs ; Escherichia coli ; Foodborne pathogens ; Gastroenteritis ; Gene expression ; Hemolytic uremic syndrome ; Hemorrhage ; Microorganisms ; Minimum inhibitory concentration ; Multidrug resistance ; Multidrug resistant organisms ; Nanoparticles ; O26 ; Phenotypes ; protein expression ; Proteins ; SEM ; Shiga toxin ; Stx2 gene ; Toxins ; Zinc oxide ; Zinc oxides ; ZnO NPs</subject><ispartof>Annals of animal science, 2023-04, Vol.23 (2), p.461-471</ispartof><rights>2023. This work is published under http://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-a23302fc763d62c366e45b8436e7da667fe1a8cbbe20bdf0e22af50c25b4d08b3</citedby><cites>FETCH-LOGICAL-c290t-a23302fc763d62c366e45b8436e7da667fe1a8cbbe20bdf0e22af50c25b4d08b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://sciendo.com/pdf/10.2478/aoas-2022-0088$$EPDF$$P50$$Gwalterdegruyter$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://sciendo.com/article/10.2478/aoas-2022-0088$$EHTML$$P50$$Gwalterdegruyter$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,76164,76165</link.rule.ids></links><search><creatorcontrib>Sherif, Rasha M.</creatorcontrib><creatorcontrib>Talat, Dalia</creatorcontrib><creatorcontrib>Alaidaroos, Bothaina A.</creatorcontrib><creatorcontrib>Farsi, Reem M.</creatorcontrib><creatorcontrib>Hassoubah, Shahira A.</creatorcontrib><creatorcontrib>Jaber, Fatima A.</creatorcontrib><creatorcontrib>Azer, Treza M.</creatorcontrib><creatorcontrib>El-Masry, Reham M.</creatorcontrib><creatorcontrib>Abd El-Hack, Mohamed E.</creatorcontrib><creatorcontrib>Ibrahim, Madiha S.</creatorcontrib><creatorcontrib>Elbestawy, Ahmed</creatorcontrib><title>Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)</title><title>Annals of animal science</title><description>The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing
is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against
O26 and its Shiga toxin type 2 (
). Multidrug resistance phenotype was observed in
O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of
O26 (10
, 10
and 10
CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in
level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the
gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in
O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on
O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against
O26.</description><subject>Antibacterial activity</subject><subject>Antibacterial agents</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Cell density</subject><subject>Colitis</subject><subject>E coli</subject><subject>Electron micrographs</subject><subject>Escherichia coli</subject><subject>Foodborne pathogens</subject><subject>Gastroenteritis</subject><subject>Gene expression</subject><subject>Hemolytic uremic syndrome</subject><subject>Hemorrhage</subject><subject>Microorganisms</subject><subject>Minimum inhibitory concentration</subject><subject>Multidrug resistance</subject><subject>Multidrug resistant organisms</subject><subject>Nanoparticles</subject><subject>O26</subject><subject>Phenotypes</subject><subject>protein expression</subject><subject>Proteins</subject><subject>SEM</subject><subject>Shiga toxin</subject><subject>Stx2 gene</subject><subject>Toxins</subject><subject>Zinc oxide</subject><subject>Zinc oxides</subject><subject>ZnO NPs</subject><issn>2300-8733</issn><issn>1642-3402</issn><issn>2300-8733</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptUEtLAzEQDqJgqb16DnjRw9bsZDcbwUsp9QGFHtRzyGazbco2WZMsWn-9KRX04Fxm4HvMzIfQZU6mUFT8VjoZMiAAGSGcn6ARUEIyXlF6-mc-R5MQtiRVWTDG6QhtZzaanVHe1UZ22Ox6qWLArsVfxirsPk2jsZXW9dJHozqdMItfNmYtcUyozXrvmkEZu8aLoDbaG7UxEivXGXwdtHdx32u8AnZzgc5a2QU9-elj9PaweJ0_ZcvV4_N8tswU3JGYSaCUQKsqRhsGijKmi7LmBWW6aiRjVatzyVVdayB10xININuSKCjroiG8pmN0dfRNl70POkSxdYO3aaUATnhJy5IViTU9stLrIXjdit6bnfR7kRNxiFQcIhWHSMUh0iS4Pwo-ZBe1b_TaD_s0_Lr_LwQKBcvpN9tOfq4</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Sherif, Rasha M.</creator><creator>Talat, Dalia</creator><creator>Alaidaroos, Bothaina A.</creator><creator>Farsi, Reem M.</creator><creator>Hassoubah, Shahira A.</creator><creator>Jaber, Fatima A.</creator><creator>Azer, Treza M.</creator><creator>El-Masry, Reham M.</creator><creator>Abd El-Hack, Mohamed E.</creator><creator>Ibrahim, Madiha S.</creator><creator>Elbestawy, Ahmed</creator><general>Sciendo</general><general>De Gruyter Poland</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>H94</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20230401</creationdate><title>Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)</title><author>Sherif, Rasha M. ; Talat, Dalia ; Alaidaroos, Bothaina A. ; Farsi, Reem M. ; Hassoubah, Shahira A. ; Jaber, Fatima A. ; Azer, Treza M. ; El-Masry, Reham M. ; Abd El-Hack, Mohamed E. ; Ibrahim, Madiha S. ; Elbestawy, Ahmed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-a23302fc763d62c366e45b8436e7da667fe1a8cbbe20bdf0e22af50c25b4d08b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antibacterial activity</topic><topic>Antibacterial agents</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Cell density</topic><topic>Colitis</topic><topic>E coli</topic><topic>Electron micrographs</topic><topic>Escherichia coli</topic><topic>Foodborne pathogens</topic><topic>Gastroenteritis</topic><topic>Gene expression</topic><topic>Hemolytic uremic syndrome</topic><topic>Hemorrhage</topic><topic>Microorganisms</topic><topic>Minimum inhibitory concentration</topic><topic>Multidrug resistance</topic><topic>Multidrug resistant organisms</topic><topic>Nanoparticles</topic><topic>O26</topic><topic>Phenotypes</topic><topic>protein expression</topic><topic>Proteins</topic><topic>SEM</topic><topic>Shiga toxin</topic><topic>Stx2 gene</topic><topic>Toxins</topic><topic>Zinc oxide</topic><topic>Zinc oxides</topic><topic>ZnO NPs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sherif, Rasha M.</creatorcontrib><creatorcontrib>Talat, Dalia</creatorcontrib><creatorcontrib>Alaidaroos, Bothaina A.</creatorcontrib><creatorcontrib>Farsi, Reem M.</creatorcontrib><creatorcontrib>Hassoubah, Shahira A.</creatorcontrib><creatorcontrib>Jaber, Fatima A.</creatorcontrib><creatorcontrib>Azer, Treza M.</creatorcontrib><creatorcontrib>El-Masry, Reham M.</creatorcontrib><creatorcontrib>Abd El-Hack, Mohamed E.</creatorcontrib><creatorcontrib>Ibrahim, Madiha S.</creatorcontrib><creatorcontrib>Elbestawy, Ahmed</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Annals of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sherif, Rasha M.</au><au>Talat, Dalia</au><au>Alaidaroos, Bothaina A.</au><au>Farsi, Reem M.</au><au>Hassoubah, Shahira A.</au><au>Jaber, Fatima A.</au><au>Azer, Treza M.</au><au>El-Masry, Reham M.</au><au>Abd El-Hack, Mohamed E.</au><au>Ibrahim, Madiha S.</au><au>Elbestawy, Ahmed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26)</atitle><jtitle>Annals of animal science</jtitle><date>2023-04-01</date><risdate>2023</risdate><volume>23</volume><issue>2</issue><spage>461</spage><epage>471</epage><pages>461-471</pages><issn>2300-8733</issn><issn>1642-3402</issn><eissn>2300-8733</eissn><abstract>The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing
is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against
O26 and its Shiga toxin type 2 (
). Multidrug resistance phenotype was observed in
O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of
O26 (10
, 10
and 10
CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in
level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the
gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in
O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on
O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against
O26.</abstract><cop>Kraków</cop><pub>Sciendo</pub><doi>10.2478/aoas-2022-0088</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of animal science, 2023-04, Vol.23 (2), p.461-471 |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Walter De Gruyter: Open Access Journals; Alma/SFX Local Collection |
| subjects | Antibacterial activity Antibacterial agents Antimicrobial activity Antimicrobial agents Bacteria Cell density Colitis E coli Electron micrographs Escherichia coli Foodborne pathogens Gastroenteritis Gene expression Hemolytic uremic syndrome Hemorrhage Microorganisms Minimum inhibitory concentration Multidrug resistance Multidrug resistant organisms Nanoparticles O26 Phenotypes protein expression Proteins SEM Shiga toxin Stx2 gene Toxins Zinc oxide Zinc oxides ZnO NPs |
| title | Antimicrobial impacts of zinc oxide nanoparticles on Shiga toxin-producing Escherichia coli (serotype O26) |
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