Quantifying Production of Quorum Sensing Regulated Pigments in Pseudomonas aeruginosa BIOTECH 1335

One more method that can be used in the fight against communicable diseases is greatly important. Numerous pathogenic bacteria use intercellular signaling known as quorum sensing (QS) in defining virulence gene expression as well as gene regulatory mechanisms. Among the most promising sources of QSI agents are the ethnobotanicals. Extraction used 95% n-hexane in ethnobotanical leaves of A. triplinervis, B. pilosa, C. nocturnum, S. glabra, P. pentandrum, O. trinervis, D. elliptica, A. scholaris, A. adenophora, and Lipang daga (no scientific name). Extracts that were negative in the antibacterial testing proceeded to the QSI assay for pyocyanin production. The ten ethnobotanical extracts did not exhibit antibacterial activity against P. aeruginosa and were found to increase the pyocyanin production therefore the absence of QSI. However, all plant extracts can be used to increase the production of pyocyanin to accumulate more metabolites that are proven to have important biological and biotechnological applications.