Investigation of hemocompatibility and physicochemical properties of acellular caprine pericardium for biomedical applications

In this study, the combination of sodium dodecyl sulfate (SDS) and Triton X-100 was used to investigate the hemocompatibility and physicochemical properties of the native and decellularized caprine pericardium (DCP). Histology and infrared spectroscopy were used to characterize the DCP. The DCP was sterilized with ethanol and antibiotics, and the detergent’s residual cytotoxicity was investigated. The native pericardium and DCP were then tested for hemocompatibility and in vitro enzyme biodegradability. The histology shows no cell nuclei with intact matrix, while infrared spectroscopy shows identical peaks in DCP and native pericardium and antibiotic treatment only make DCP completely sterile. The SDS and Triton X-100 detergents are cytotoxic, and the cytotoxicity was significantly reduced by the addition of washing steps. According to the hemocompatibility study, the hemolytic indices of native and DCP are 2% and 5%. DCP showed higher degradation resistance with the trypsin enzyme than with the collagenase. Thus the scaffold created from this xenogeneic model has promising qualities that are useful for tissue engineering applications. Graphical abstract