Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin
Caralluma umbellata Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an...
Gespeichert in:
| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2023-04, Vol.153 (1), p.105-118 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 118 |
|---|---|
| container_issue | 1 |
| container_start_page | 105 |
| container_title | Plant cell, tissue and organ culture |
| container_volume | 153 |
| creator | Jayaprakash, K. Manokari, M. Cokulraj, M. Dey, Abhijit Faisal, Mohammad Alatar, Abdulrahman A. Joshee, Nirmal Shekhawat, Mahipal S. |
| description | Caralluma umbellata
Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an effective in vitro propagation system was developed using 6-(3-hydroxybenzylamino) purine (
Meta
-Topolin;
m
T) for rapid clonal production of
C. umbellata
planting material. The highest shoot induction frequency (93.0%) and multiple shoot regeneration (6.0 shoots/nodal explants) was achieved on Murashige and Skoog’s (MS) medium fortified with 2.0 mg L
−1
of
m
T in 4 weeks. The synergistic impact of 1.0 mg L
−1
m
T and 0.25 mg L
−1
NAA (α-Naphthalene acetic acid) was evident in shoot proliferation (43.6 shoots per nodal explant with 6.2 cm length after 3rd subculture) as compared to 6-benzylaminopurine (BAP) and NAA combinations. The structural traits concerning optimized plant growth regulators (PGRs) were enumerated from the in vitro raised shootlets. Microscopic results revealed that
m
T and NAA combination promoted the structural developments in shoot systems with well-developed cuticle, epidermis, and the presence of a large area of ground and vascular tissues. Whereas, the shoots developed on BAP and NAA had poorly developed tissue systems with thin cuticle, underdeveloped epidermis, reduced cortex, xylem, and phloem tissues. The shoots derived from
m
T and NAA supplemented medium exhibited 100% rooting averaging with 4.0 roots per shoot and 2.7 cm in length on half-strength MS medium containing 1.0 mg L
−1
indole-3-butyric acid (IBA). In contrast, BAP and NAA-derived shoots exhibited 75% rooting with 3 roots per shoot averaging 1.9 cm in length on 1.0 mg L
−1
IBA. The plantlets were hardened and acclimatized well in greenhouse and shade-net. The plantlets derived from
m
T and NAA presented a significant increase in survival rate (94%) after transplantation in the field as compared to BAP and NAA-derived plantlets (81%). The presence of
m
T in the medium reduced structural abnormalities and mortality that may better serve the purpose of germplasm conservation, large-scale production, and the sustainable utilization of
C. umbellata
as a medicinal plant.
Key message
An improved shoot proliferation with well-developed tissue systems could be obtained in
Caralluma umbellata
by selecting 6-(3-hydroxybenzylamino) purine as a growth regulator, which reduced in vi |
| doi_str_mv | 10.1007/s11240-023-02447-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2799290675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2799290675</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-90b91848ab56d3ed469a8cb985bc71146925b9cca7fe96e0caba5d87ff9f7b043</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMouH78AU8Bz9FJmzbNURZ1BcWLnsO0TZcubVKTVPEX-LfNWsGbh2EY5n3m4yXkgsMVB5DXgfNMAIMsTyGEZHBAVryQOStAiEOyAl5KVlaFPCYnIewAoMwFX5Gvh3Hy7t201PktWrc11oQ-ULQtHfvGOxain5s4exxo9NjHQHu7tBI44RZjgqcBbRxMarqOrjGJh3lEOo-1GQaMSDf4cbUHvQmTs8HQ6OiTiche3OSG3p6Row6HYM5_8yl5vbt9WW_Y4_P9w_rmkTU5V5EpqBWvRIV1Uba5aUWpsGpqVRV1IzlPZVbUqmlQdkaVBhqssWgr2XWqkzWI_JRcLnPT8W-zCVHv3OxtWqkzqVSmoJRFUmWLKn0Zgjednnw_ov_UHPTecL0YrpPh-sdwDQnKFygksd0a_zf6H-ob88iG2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2799290675</pqid></control><display><type>article</type><title>Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin</title><source>SpringerLink Journals</source><creator>Jayaprakash, K. ; Manokari, M. ; Cokulraj, M. ; Dey, Abhijit ; Faisal, Mohammad ; Alatar, Abdulrahman A. ; Joshee, Nirmal ; Shekhawat, Mahipal S.</creator><creatorcontrib>Jayaprakash, K. ; Manokari, M. ; Cokulraj, M. ; Dey, Abhijit ; Faisal, Mohammad ; Alatar, Abdulrahman A. ; Joshee, Nirmal ; Shekhawat, Mahipal S.</creatorcontrib><description>Caralluma umbellata
Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an effective in vitro propagation system was developed using 6-(3-hydroxybenzylamino) purine (
Meta
-Topolin;
m
T) for rapid clonal production of
C. umbellata
planting material. The highest shoot induction frequency (93.0%) and multiple shoot regeneration (6.0 shoots/nodal explants) was achieved on Murashige and Skoog’s (MS) medium fortified with 2.0 mg L
−1
of
m
T in 4 weeks. The synergistic impact of 1.0 mg L
−1
m
T and 0.25 mg L
−1
NAA (α-Naphthalene acetic acid) was evident in shoot proliferation (43.6 shoots per nodal explant with 6.2 cm length after 3rd subculture) as compared to 6-benzylaminopurine (BAP) and NAA combinations. The structural traits concerning optimized plant growth regulators (PGRs) were enumerated from the in vitro raised shootlets. Microscopic results revealed that
m
T and NAA combination promoted the structural developments in shoot systems with well-developed cuticle, epidermis, and the presence of a large area of ground and vascular tissues. Whereas, the shoots developed on BAP and NAA had poorly developed tissue systems with thin cuticle, underdeveloped epidermis, reduced cortex, xylem, and phloem tissues. The shoots derived from
m
T and NAA supplemented medium exhibited 100% rooting averaging with 4.0 roots per shoot and 2.7 cm in length on half-strength MS medium containing 1.0 mg L
−1
indole-3-butyric acid (IBA). In contrast, BAP and NAA-derived shoots exhibited 75% rooting with 3 roots per shoot averaging 1.9 cm in length on 1.0 mg L
−1
IBA. The plantlets were hardened and acclimatized well in greenhouse and shade-net. The plantlets derived from
m
T and NAA presented a significant increase in survival rate (94%) after transplantation in the field as compared to BAP and NAA-derived plantlets (81%). The presence of
m
T in the medium reduced structural abnormalities and mortality that may better serve the purpose of germplasm conservation, large-scale production, and the sustainable utilization of
C. umbellata
as a medicinal plant.
Key message
An improved shoot proliferation with well-developed tissue systems could be obtained in
Caralluma umbellata
by selecting 6-(3-hydroxybenzylamino) purine as a growth regulator, which reduced in vitro induced abnormalities and improved the survival of plantlets.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02447-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abnormalities ; Acclimatization ; Acetic acid ; Benzyladenine ; Biomedical and Life Sciences ; Butyric acid ; Caralluma umbellata ; Epidermis ; Explants ; Germplasm ; Growth regulators ; Herbal medicine ; Indole-3-butyric acid ; Life Sciences ; Medicinal plants ; Naphthalene ; Organogenesis ; Original Article ; Plant cuticle ; Plant Genetics and Genomics ; Plant growth ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Plant tissues ; Plantlets ; Propagation ; Rooting ; Roots ; Shoots ; Subculture ; Survival ; Transplantation ; Vascular tissue ; Weight control ; Weight loss ; Xylem</subject><ispartof>Plant cell, tissue and organ culture, 2023-04, Vol.153 (1), p.105-118</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-90b91848ab56d3ed469a8cb985bc71146925b9cca7fe96e0caba5d87ff9f7b043</citedby><cites>FETCH-LOGICAL-c319t-90b91848ab56d3ed469a8cb985bc71146925b9cca7fe96e0caba5d87ff9f7b043</cites><orcidid>0000-0002-0990-8766</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02447-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02447-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Jayaprakash, K.</creatorcontrib><creatorcontrib>Manokari, M.</creatorcontrib><creatorcontrib>Cokulraj, M.</creatorcontrib><creatorcontrib>Dey, Abhijit</creatorcontrib><creatorcontrib>Faisal, Mohammad</creatorcontrib><creatorcontrib>Alatar, Abdulrahman A.</creatorcontrib><creatorcontrib>Joshee, Nirmal</creatorcontrib><creatorcontrib>Shekhawat, Mahipal S.</creatorcontrib><title>Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Caralluma umbellata
Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an effective in vitro propagation system was developed using 6-(3-hydroxybenzylamino) purine (
Meta
-Topolin;
m
T) for rapid clonal production of
C. umbellata
planting material. The highest shoot induction frequency (93.0%) and multiple shoot regeneration (6.0 shoots/nodal explants) was achieved on Murashige and Skoog’s (MS) medium fortified with 2.0 mg L
−1
of
m
T in 4 weeks. The synergistic impact of 1.0 mg L
−1
m
T and 0.25 mg L
−1
NAA (α-Naphthalene acetic acid) was evident in shoot proliferation (43.6 shoots per nodal explant with 6.2 cm length after 3rd subculture) as compared to 6-benzylaminopurine (BAP) and NAA combinations. The structural traits concerning optimized plant growth regulators (PGRs) were enumerated from the in vitro raised shootlets. Microscopic results revealed that
m
T and NAA combination promoted the structural developments in shoot systems with well-developed cuticle, epidermis, and the presence of a large area of ground and vascular tissues. Whereas, the shoots developed on BAP and NAA had poorly developed tissue systems with thin cuticle, underdeveloped epidermis, reduced cortex, xylem, and phloem tissues. The shoots derived from
m
T and NAA supplemented medium exhibited 100% rooting averaging with 4.0 roots per shoot and 2.7 cm in length on half-strength MS medium containing 1.0 mg L
−1
indole-3-butyric acid (IBA). In contrast, BAP and NAA-derived shoots exhibited 75% rooting with 3 roots per shoot averaging 1.9 cm in length on 1.0 mg L
−1
IBA. The plantlets were hardened and acclimatized well in greenhouse and shade-net. The plantlets derived from
m
T and NAA presented a significant increase in survival rate (94%) after transplantation in the field as compared to BAP and NAA-derived plantlets (81%). The presence of
m
T in the medium reduced structural abnormalities and mortality that may better serve the purpose of germplasm conservation, large-scale production, and the sustainable utilization of
C. umbellata
as a medicinal plant.
Key message
An improved shoot proliferation with well-developed tissue systems could be obtained in
Caralluma umbellata
by selecting 6-(3-hydroxybenzylamino) purine as a growth regulator, which reduced in vitro induced abnormalities and improved the survival of plantlets.</description><subject>Abnormalities</subject><subject>Acclimatization</subject><subject>Acetic acid</subject><subject>Benzyladenine</subject><subject>Biomedical and Life Sciences</subject><subject>Butyric acid</subject><subject>Caralluma umbellata</subject><subject>Epidermis</subject><subject>Explants</subject><subject>Germplasm</subject><subject>Growth regulators</subject><subject>Herbal medicine</subject><subject>Indole-3-butyric acid</subject><subject>Life Sciences</subject><subject>Medicinal plants</subject><subject>Naphthalene</subject><subject>Organogenesis</subject><subject>Original Article</subject><subject>Plant cuticle</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plant tissues</subject><subject>Plantlets</subject><subject>Propagation</subject><subject>Rooting</subject><subject>Roots</subject><subject>Shoots</subject><subject>Subculture</subject><subject>Survival</subject><subject>Transplantation</subject><subject>Vascular tissue</subject><subject>Weight control</subject><subject>Weight loss</subject><subject>Xylem</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LxDAQhoMouH78AU8Bz9FJmzbNURZ1BcWLnsO0TZcubVKTVPEX-LfNWsGbh2EY5n3m4yXkgsMVB5DXgfNMAIMsTyGEZHBAVryQOStAiEOyAl5KVlaFPCYnIewAoMwFX5Gvh3Hy7t201PktWrc11oQ-ULQtHfvGOxain5s4exxo9NjHQHu7tBI44RZjgqcBbRxMarqOrjGJh3lEOo-1GQaMSDf4cbUHvQmTs8HQ6OiTiche3OSG3p6Row6HYM5_8yl5vbt9WW_Y4_P9w_rmkTU5V5EpqBWvRIV1Uba5aUWpsGpqVRV1IzlPZVbUqmlQdkaVBhqssWgr2XWqkzWI_JRcLnPT8W-zCVHv3OxtWqkzqVSmoJRFUmWLKn0Zgjednnw_ov_UHPTecL0YrpPh-sdwDQnKFygksd0a_zf6H-ob88iG2Q</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Jayaprakash, K.</creator><creator>Manokari, M.</creator><creator>Cokulraj, M.</creator><creator>Dey, Abhijit</creator><creator>Faisal, Mohammad</creator><creator>Alatar, Abdulrahman A.</creator><creator>Joshee, Nirmal</creator><creator>Shekhawat, Mahipal S.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0002-0990-8766</orcidid></search><sort><creationdate>20230401</creationdate><title>Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin</title><author>Jayaprakash, K. ; Manokari, M. ; Cokulraj, M. ; Dey, Abhijit ; Faisal, Mohammad ; Alatar, Abdulrahman A. ; Joshee, Nirmal ; Shekhawat, Mahipal S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-90b91848ab56d3ed469a8cb985bc71146925b9cca7fe96e0caba5d87ff9f7b043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abnormalities</topic><topic>Acclimatization</topic><topic>Acetic acid</topic><topic>Benzyladenine</topic><topic>Biomedical and Life Sciences</topic><topic>Butyric acid</topic><topic>Caralluma umbellata</topic><topic>Epidermis</topic><topic>Explants</topic><topic>Germplasm</topic><topic>Growth regulators</topic><topic>Herbal medicine</topic><topic>Indole-3-butyric acid</topic><topic>Life Sciences</topic><topic>Medicinal plants</topic><topic>Naphthalene</topic><topic>Organogenesis</topic><topic>Original Article</topic><topic>Plant cuticle</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plant tissues</topic><topic>Plantlets</topic><topic>Propagation</topic><topic>Rooting</topic><topic>Roots</topic><topic>Shoots</topic><topic>Subculture</topic><topic>Survival</topic><topic>Transplantation</topic><topic>Vascular tissue</topic><topic>Weight control</topic><topic>Weight loss</topic><topic>Xylem</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jayaprakash, K.</creatorcontrib><creatorcontrib>Manokari, M.</creatorcontrib><creatorcontrib>Cokulraj, M.</creatorcontrib><creatorcontrib>Dey, Abhijit</creatorcontrib><creatorcontrib>Faisal, Mohammad</creatorcontrib><creatorcontrib>Alatar, Abdulrahman A.</creatorcontrib><creatorcontrib>Joshee, Nirmal</creatorcontrib><creatorcontrib>Shekhawat, Mahipal S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jayaprakash, K.</au><au>Manokari, M.</au><au>Cokulraj, M.</au><au>Dey, Abhijit</au><au>Faisal, Mohammad</au><au>Alatar, Abdulrahman A.</au><au>Joshee, Nirmal</au><au>Shekhawat, Mahipal S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2023-04-01</date><risdate>2023</risdate><volume>153</volume><issue>1</issue><spage>105</spage><epage>118</epage><pages>105-118</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Caralluma umbellata
Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an effective in vitro propagation system was developed using 6-(3-hydroxybenzylamino) purine (
Meta
-Topolin;
m
T) for rapid clonal production of
C. umbellata
planting material. The highest shoot induction frequency (93.0%) and multiple shoot regeneration (6.0 shoots/nodal explants) was achieved on Murashige and Skoog’s (MS) medium fortified with 2.0 mg L
−1
of
m
T in 4 weeks. The synergistic impact of 1.0 mg L
−1
m
T and 0.25 mg L
−1
NAA (α-Naphthalene acetic acid) was evident in shoot proliferation (43.6 shoots per nodal explant with 6.2 cm length after 3rd subculture) as compared to 6-benzylaminopurine (BAP) and NAA combinations. The structural traits concerning optimized plant growth regulators (PGRs) were enumerated from the in vitro raised shootlets. Microscopic results revealed that
m
T and NAA combination promoted the structural developments in shoot systems with well-developed cuticle, epidermis, and the presence of a large area of ground and vascular tissues. Whereas, the shoots developed on BAP and NAA had poorly developed tissue systems with thin cuticle, underdeveloped epidermis, reduced cortex, xylem, and phloem tissues. The shoots derived from
m
T and NAA supplemented medium exhibited 100% rooting averaging with 4.0 roots per shoot and 2.7 cm in length on half-strength MS medium containing 1.0 mg L
−1
indole-3-butyric acid (IBA). In contrast, BAP and NAA-derived shoots exhibited 75% rooting with 3 roots per shoot averaging 1.9 cm in length on 1.0 mg L
−1
IBA. The plantlets were hardened and acclimatized well in greenhouse and shade-net. The plantlets derived from
m
T and NAA presented a significant increase in survival rate (94%) after transplantation in the field as compared to BAP and NAA-derived plantlets (81%). The presence of
m
T in the medium reduced structural abnormalities and mortality that may better serve the purpose of germplasm conservation, large-scale production, and the sustainable utilization of
C. umbellata
as a medicinal plant.
Key message
An improved shoot proliferation with well-developed tissue systems could be obtained in
Caralluma umbellata
by selecting 6-(3-hydroxybenzylamino) purine as a growth regulator, which reduced in vitro induced abnormalities and improved the survival of plantlets.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02447-0</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-0990-8766</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2023-04, Vol.153 (1), p.105-118 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_journals_2799290675 |
| source | SpringerLink Journals |
| subjects | Abnormalities Acclimatization Acetic acid Benzyladenine Biomedical and Life Sciences Butyric acid Caralluma umbellata Epidermis Explants Germplasm Growth regulators Herbal medicine Indole-3-butyric acid Life Sciences Medicinal plants Naphthalene Organogenesis Original Article Plant cuticle Plant Genetics and Genomics Plant growth Plant Pathology Plant Physiology Plant Sciences Plant tissues Plantlets Propagation Rooting Roots Shoots Subculture Survival Transplantation Vascular tissue Weight control Weight loss Xylem |
| title | Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T02%3A04%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20organogenesis%20and%20micro-structural%20traits%20in%20micropropagated%20plantlets%20of%20Caralluma%20umbellata%20Haw.%20in%20response%20to%20Meta-Topolin&rft.jtitle=Plant%20cell,%20tissue%20and%20organ%20culture&rft.au=Jayaprakash,%20K.&rft.date=2023-04-01&rft.volume=153&rft.issue=1&rft.spage=105&rft.epage=118&rft.pages=105-118&rft.issn=0167-6857&rft.eissn=1573-5044&rft_id=info:doi/10.1007/s11240-023-02447-0&rft_dat=%3Cproquest_cross%3E2799290675%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2799290675&rft_id=info:pmid/&rfr_iscdi=true |