Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence
Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility...
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Veröffentlicht in: | Angewandte Chemie 2023-04, Vol.135 (16), p.n/a |
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description | Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility to record ECL images of cells and mitochondria at concentrations down to nM and pM. This is 7 orders of magnitude lower than classically‐used concentrations and corresponds to a few hundreds of luminophores diffusing around the biological entities. Yet, it produces remarkably sharp negative optical contrast ECL images, as demonstrated by structural similarity index metric analyses and supported by predictions of the ECL image covering time. Finally, we show that the reported approach is a simple, fast, and highly sensitive method, which opens new avenues for ultrasensitive ECL imaging and ECL reactivity at the single molecule level.
Here, we report on label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations (nano‐ to picomolar) of the [Ru(bpy)3]2+ luminophore. This work addresses the central issue of the minimal concentration of the ECL luminophore required to image single entities such as cells or mitochondria. |
doi_str_mv | 10.1002/ange.202218574 |
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Here, we report on label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations (nano‐ to picomolar) of the [Ru(bpy)3]2+ luminophore. This work addresses the central issue of the minimal concentration of the ECL luminophore required to image single entities such as cells or mitochondria.</description><identifier>ISSN: 0044-8249</identifier><identifier>EISSN: 1521-3757</identifier><identifier>DOI: 10.1002/ange.202218574</identifier><language>eng</language><publisher>Weinheim: Wiley Subscription Services, Inc</publisher><subject>Chemistry ; Electrochemiluminescence ; Image contrast ; Low concentrations ; Mechanism ; Microscopy ; Mitochondria ; Single Cell ; Single Molecule</subject><ispartof>Angewandte Chemie, 2023-04, Vol.135 (16), p.n/a</ispartof><rights>2023 The Authors. Angewandte Chemie published by Wiley-VCH GmbH</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2684-c07d483ba294cffa34adb185e62ee158c17caabd563647123cdd2d3a96433eda3</citedby><cites>FETCH-LOGICAL-c2684-c07d483ba294cffa34adb185e62ee158c17caabd563647123cdd2d3a96433eda3</cites><orcidid>0000-0001-7518-3735 ; 0000-0002-4994-2213 ; 0000-0003-3558-925X ; 0000-0001-5144-1015</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fange.202218574$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fange.202218574$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids></links><search><creatorcontrib>Descamps, Julie</creatorcontrib><creatorcontrib>Colin, Camille</creatorcontrib><creatorcontrib>Tessier, Gilles</creatorcontrib><creatorcontrib>Arbault, Stéphane</creatorcontrib><creatorcontrib>Sojic, Neso</creatorcontrib><title>Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence</title><title>Angewandte Chemie</title><description>Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility to record ECL images of cells and mitochondria at concentrations down to nM and pM. This is 7 orders of magnitude lower than classically‐used concentrations and corresponds to a few hundreds of luminophores diffusing around the biological entities. Yet, it produces remarkably sharp negative optical contrast ECL images, as demonstrated by structural similarity index metric analyses and supported by predictions of the ECL image covering time. Finally, we show that the reported approach is a simple, fast, and highly sensitive method, which opens new avenues for ultrasensitive ECL imaging and ECL reactivity at the single molecule level.
Here, we report on label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations (nano‐ to picomolar) of the [Ru(bpy)3]2+ luminophore. This work addresses the central issue of the minimal concentration of the ECL luminophore required to image single entities such as cells or mitochondria.</description><subject>Chemistry</subject><subject>Electrochemiluminescence</subject><subject>Image contrast</subject><subject>Low concentrations</subject><subject>Mechanism</subject><subject>Microscopy</subject><subject>Mitochondria</subject><subject>Single Cell</subject><subject>Single Molecule</subject><issn>0044-8249</issn><issn>1521-3757</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNqFkLtOwzAUhi0EEqWwMltiTvEtt7GqQlupggEqRsuxT0oqxyl2AurGI_CMPAmpimBkOjrS953Lj9A1JRNKCLtVbgMTRhijWZyKEzSiMaMRT-P0FI0IESLKmMjP0UUIW0JIwtJ8hJ7XtvMqgAt1V78BXjZqU7sNbis8A2sDVs7gx778-vg89L1VHheuG2AIuNzjwoLufKtfoKlt39QOggan4RKdVcoGuPqpY7S-K55mi2j1MF_OpqtIsyQTkSapERkvFcuFrirFhTLlcD8kDIDGmaapVqo0ccITkVLGtTHMcJUngnMwio_RzXHuzrevPYRObtveu2GlHP7jWU5iKgZqcqS0b0PwUMmdrxvl95ISeQhPHsKTv-ENQn4U3msL-39oOb2fF3_uNyzMdYA</recordid><startdate>20230411</startdate><enddate>20230411</enddate><creator>Descamps, Julie</creator><creator>Colin, Camille</creator><creator>Tessier, Gilles</creator><creator>Arbault, Stéphane</creator><creator>Sojic, Neso</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0001-7518-3735</orcidid><orcidid>https://orcid.org/0000-0002-4994-2213</orcidid><orcidid>https://orcid.org/0000-0003-3558-925X</orcidid><orcidid>https://orcid.org/0000-0001-5144-1015</orcidid></search><sort><creationdate>20230411</creationdate><title>Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence</title><author>Descamps, Julie ; Colin, Camille ; Tessier, Gilles ; Arbault, Stéphane ; Sojic, Neso</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2684-c07d483ba294cffa34adb185e62ee158c17caabd563647123cdd2d3a96433eda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Chemistry</topic><topic>Electrochemiluminescence</topic><topic>Image contrast</topic><topic>Low concentrations</topic><topic>Mechanism</topic><topic>Microscopy</topic><topic>Mitochondria</topic><topic>Single Cell</topic><topic>Single Molecule</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Descamps, Julie</creatorcontrib><creatorcontrib>Colin, Camille</creatorcontrib><creatorcontrib>Tessier, Gilles</creatorcontrib><creatorcontrib>Arbault, Stéphane</creatorcontrib><creatorcontrib>Sojic, Neso</creatorcontrib><collection>Wiley Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Angewandte Chemie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Descamps, Julie</au><au>Colin, Camille</au><au>Tessier, Gilles</au><au>Arbault, Stéphane</au><au>Sojic, Neso</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence</atitle><jtitle>Angewandte Chemie</jtitle><date>2023-04-11</date><risdate>2023</risdate><volume>135</volume><issue>16</issue><epage>n/a</epage><issn>0044-8249</issn><eissn>1521-3757</eissn><abstract>Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. 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Here, we report on label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations (nano‐ to picomolar) of the [Ru(bpy)3]2+ luminophore. This work addresses the central issue of the minimal concentration of the ECL luminophore required to image single entities such as cells or mitochondria.</abstract><cop>Weinheim</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/ange.202218574</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-7518-3735</orcidid><orcidid>https://orcid.org/0000-0002-4994-2213</orcidid><orcidid>https://orcid.org/0000-0003-3558-925X</orcidid><orcidid>https://orcid.org/0000-0001-5144-1015</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Chemistry Electrochemiluminescence Image contrast Low concentrations Mechanism Microscopy Mitochondria Single Cell Single Molecule |
title | Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence |
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