Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence

Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility...

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Veröffentlicht in:Angewandte Chemie 2023-04, Vol.135 (16), p.n/a
Hauptverfasser: Descamps, Julie, Colin, Camille, Tessier, Gilles, Arbault, Stéphane, Sojic, Neso
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container_issue 16
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container_title Angewandte Chemie
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creator Descamps, Julie
Colin, Camille
Tessier, Gilles
Arbault, Stéphane
Sojic, Neso
description Here we report on a label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility to record ECL images of cells and mitochondria at concentrations down to nM and pM. This is 7 orders of magnitude lower than classically‐used concentrations and corresponds to a few hundreds of luminophores diffusing around the biological entities. Yet, it produces remarkably sharp negative optical contrast ECL images, as demonstrated by structural similarity index metric analyses and supported by predictions of the ECL image covering time. Finally, we show that the reported approach is a simple, fast, and highly sensitive method, which opens new avenues for ultrasensitive ECL imaging and ECL reactivity at the single molecule level. Here, we report on label‐free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations (nano‐ to picomolar) of the [Ru(bpy)3]2+ luminophore. This work addresses the central issue of the minimal concentration of the ECL luminophore required to image single entities such as cells or mitochondria.
doi_str_mv 10.1002/ange.202218574
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subjects Chemistry
Electrochemiluminescence
Image contrast
Low concentrations
Mechanism
Microscopy
Mitochondria
Single Cell
Single Molecule
title Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence
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