In-vitro antimicrobial & biocompatibility study of Spherical 52S4.6 Submicron-Bioactive glass synthesized by Stöber Method: Effect of Ag Doping
Bioactive glass has become a promising material due to its biocompatibility and bioactivity toward both soft tissue and hard tissue regeneration. In the present study, nearly monodisperse, dense and spherical shaped un-doped and Ag-doped 52S4.6 submicron bioactive glass (SBG) particles were synthesi...
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| Veröffentlicht in: | Journal of sol-gel science and technology 2023-04, Vol.106 (1), p.67-84 |
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| description | Bioactive glass has become a promising material due to its biocompatibility and bioactivity toward both soft tissue and hard tissue regeneration. In the present study, nearly monodisperse, dense and spherical shaped un-doped and Ag-doped 52S4.6 submicron bioactive glass (SBG) particles were synthesized using sol-gel method with one-step base condensation i.e., Stöber method using tetraethyl orthosilicate (TEOS), calcium nitrate tetrahydrate (Ca(NO
3
)
2
•4H
2
O), triethyl phosphate (TEP), silver nitrate (AgNO
3
) and sodium nitrate (NaNO
3
) as precursors. The silver doping was performed at a concentration of 0.5 mole% and 1 mole% of total glass composition by changing the concentration of sodium nitrate in the bioactive glass network. The synthesized 52S4.6 SBG, both un-doped and Ag-doped powders showed predominately amorphous phase as suggested by the XRD data and indicated the presence of all the corresponding functional groups in the FT-IR spectra analysis. The synthesized powder exhibited spherical shape within the range of 448–671 nm as obtained from FE-SEM image. The synthesized 52S4.6 SBG elicited antimicrobial property against
Staphylococcus aureus
(
S. aureus
) and
Escherichia coli
(
E. coli
) at a concentration of 5 mg/ml and with an efficacy of 58.6% after 16 h incubation period. However, upon incorporation of Ag into the bioactive glass matrix efficacy in inhibiting the growth of
S. aureus
and
E. coli
was significantly enhanced to 99.6% and 99.2% with Minimum Inhibitory Concentration (MIC) of 10 mg/ml and 15 mg/ml, respectively at 0.5 mole% Ag-doped SBG at the same incubation interval. In-vitro cell culture study on biocompatibility and viability, namely live/dead assay and cell viability and proliferation assay such as MTT assay on MG-63 cell cultured Ag-doped and un-doped 52S4.6 SBG revealed that 0.5 mole% Ag-doped SBG exerted minimal cytotoxicity towards the cultured MG-63 cells.
Graphical Abstract
Highlights
Monodispersed, and spherical-shaped 52S4.6 bioactive glass (SBG) particles were synthesized.
0.5 mol% Ag-doped 52S4.6 SBG showed excellent bioactivity.
Minimum inhibitory concentrations for
E.coli
and
S. aureus
were 0.5 mole% Ag-doped 52S4.6 SBG at 10 mg/ml and 15 mg/ml, respectively. |
| doi_str_mv | 10.1007/s10971-023-06064-3 |
| format | Article |
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3
)
2
•4H
2
O), triethyl phosphate (TEP), silver nitrate (AgNO
3
) and sodium nitrate (NaNO
3
) as precursors. The silver doping was performed at a concentration of 0.5 mole% and 1 mole% of total glass composition by changing the concentration of sodium nitrate in the bioactive glass network. The synthesized 52S4.6 SBG, both un-doped and Ag-doped powders showed predominately amorphous phase as suggested by the XRD data and indicated the presence of all the corresponding functional groups in the FT-IR spectra analysis. The synthesized powder exhibited spherical shape within the range of 448–671 nm as obtained from FE-SEM image. The synthesized 52S4.6 SBG elicited antimicrobial property against
Staphylococcus aureus
(
S. aureus
) and
Escherichia coli
(
E. coli
) at a concentration of 5 mg/ml and with an efficacy of 58.6% after 16 h incubation period. However, upon incorporation of Ag into the bioactive glass matrix efficacy in inhibiting the growth of
S. aureus
and
E. coli
was significantly enhanced to 99.6% and 99.2% with Minimum Inhibitory Concentration (MIC) of 10 mg/ml and 15 mg/ml, respectively at 0.5 mole% Ag-doped SBG at the same incubation interval. In-vitro cell culture study on biocompatibility and viability, namely live/dead assay and cell viability and proliferation assay such as MTT assay on MG-63 cell cultured Ag-doped and un-doped 52S4.6 SBG revealed that 0.5 mole% Ag-doped SBG exerted minimal cytotoxicity towards the cultured MG-63 cells.
Graphical Abstract
Highlights
Monodispersed, and spherical-shaped 52S4.6 bioactive glass (SBG) particles were synthesized.
0.5 mol% Ag-doped 52S4.6 SBG showed excellent bioactivity.
Minimum inhibitory concentrations for
E.coli
and
S. aureus
were 0.5 mole% Ag-doped 52S4.6 SBG at 10 mg/ml and 15 mg/ml, respectively.</description><identifier>ISSN: 0928-0707</identifier><identifier>EISSN: 1573-4846</identifier><identifier>DOI: 10.1007/s10971-023-06064-3</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antiinfectives and antibacterials ; Assaying ; Biocompatibility ; Bioglass ; Biological activity ; Calcium nitrate ; Ceramics ; Chemistry and Materials Science ; Composites ; Condensates ; Doping ; E coli ; Effectiveness ; Functional groups ; Glass ; Infrared spectroscopy ; Inorganic Chemistry ; Materials Science ; Nanotechnology ; Natural Materials ; Nitrates ; Optical and Electronic Materials ; Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications ; Regeneration (physiology) ; Silver nitrate ; Sodium nitrates ; Soft tissues ; Sol-gel processes ; Spherical powders ; Synthesis ; Tetraethyl orthosilicate ; Tissue engineering ; Toxicity</subject><ispartof>Journal of sol-gel science and technology, 2023-04, Vol.106 (1), p.67-84</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-8b7d3297500c674ea6e179dcbd92e403238e4aca95cc253f50bfea3ca043038c3</citedby><cites>FETCH-LOGICAL-c319t-8b7d3297500c674ea6e179dcbd92e403238e4aca95cc253f50bfea3ca043038c3</cites><orcidid>0000-0002-9370-8862</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10971-023-06064-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10971-023-06064-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Ray, Sambit</creatorcontrib><creatorcontrib>Yadav, Jayprakash</creatorcontrib><creatorcontrib>Mishra, Amrita</creatorcontrib><creatorcontrib>Dasgupta, Sudip</creatorcontrib><title>In-vitro antimicrobial & biocompatibility study of Spherical 52S4.6 Submicron-Bioactive glass synthesized by Stöber Method: Effect of Ag Doping</title><title>Journal of sol-gel science and technology</title><addtitle>J Sol-Gel Sci Technol</addtitle><description>Bioactive glass has become a promising material due to its biocompatibility and bioactivity toward both soft tissue and hard tissue regeneration. In the present study, nearly monodisperse, dense and spherical shaped un-doped and Ag-doped 52S4.6 submicron bioactive glass (SBG) particles were synthesized using sol-gel method with one-step base condensation i.e., Stöber method using tetraethyl orthosilicate (TEOS), calcium nitrate tetrahydrate (Ca(NO
3
)
2
•4H
2
O), triethyl phosphate (TEP), silver nitrate (AgNO
3
) and sodium nitrate (NaNO
3
) as precursors. The silver doping was performed at a concentration of 0.5 mole% and 1 mole% of total glass composition by changing the concentration of sodium nitrate in the bioactive glass network. The synthesized 52S4.6 SBG, both un-doped and Ag-doped powders showed predominately amorphous phase as suggested by the XRD data and indicated the presence of all the corresponding functional groups in the FT-IR spectra analysis. The synthesized powder exhibited spherical shape within the range of 448–671 nm as obtained from FE-SEM image. The synthesized 52S4.6 SBG elicited antimicrobial property against
Staphylococcus aureus
(
S. aureus
) and
Escherichia coli
(
E. coli
) at a concentration of 5 mg/ml and with an efficacy of 58.6% after 16 h incubation period. However, upon incorporation of Ag into the bioactive glass matrix efficacy in inhibiting the growth of
S. aureus
and
E. coli
was significantly enhanced to 99.6% and 99.2% with Minimum Inhibitory Concentration (MIC) of 10 mg/ml and 15 mg/ml, respectively at 0.5 mole% Ag-doped SBG at the same incubation interval. In-vitro cell culture study on biocompatibility and viability, namely live/dead assay and cell viability and proliferation assay such as MTT assay on MG-63 cell cultured Ag-doped and un-doped 52S4.6 SBG revealed that 0.5 mole% Ag-doped SBG exerted minimal cytotoxicity towards the cultured MG-63 cells.
Graphical Abstract
Highlights
Monodispersed, and spherical-shaped 52S4.6 bioactive glass (SBG) particles were synthesized.
0.5 mol% Ag-doped 52S4.6 SBG showed excellent bioactivity.
Minimum inhibitory concentrations for
E.coli
and
S. aureus
were 0.5 mole% Ag-doped 52S4.6 SBG at 10 mg/ml and 15 mg/ml, respectively.</description><subject>Antiinfectives and antibacterials</subject><subject>Assaying</subject><subject>Biocompatibility</subject><subject>Bioglass</subject><subject>Biological activity</subject><subject>Calcium nitrate</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Condensates</subject><subject>Doping</subject><subject>E coli</subject><subject>Effectiveness</subject><subject>Functional groups</subject><subject>Glass</subject><subject>Infrared spectroscopy</subject><subject>Inorganic Chemistry</subject><subject>Materials Science</subject><subject>Nanotechnology</subject><subject>Natural Materials</subject><subject>Nitrates</subject><subject>Optical and Electronic Materials</subject><subject>Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications</subject><subject>Regeneration (physiology)</subject><subject>Silver nitrate</subject><subject>Sodium nitrates</subject><subject>Soft tissues</subject><subject>Sol-gel processes</subject><subject>Spherical powders</subject><subject>Synthesis</subject><subject>Tetraethyl orthosilicate</subject><subject>Tissue engineering</subject><subject>Toxicity</subject><issn>0928-0707</issn><issn>1573-4846</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kM1OGzEURq0KpIbQF-jKUqXuDNc_Mx53x18BiYpF2rVlezyJUTIebCfS8BR9mr4AL9YhqcSuK2_O-a58EPpM4YwCyPNMQUlKgHECNdSC8A9oRivJiWhEfYRmoFhDQIL8iE5yfgKASlA5Q7_ve7ILJUVs-hI2waVog1njr9iG6OJmMCXYsA5lxLls2xHHDi-GlU_BTVTFFuKsxout3Zs9uQzRuBJ2Hi_XJmecx76sfA4vvsV2xIvy-sf6hH_4sortN3zTdd6Vt82LJb6OQ-iXp-i4M-vsP_175-jX95ufV3fk4fH2_urigThOVSGNlS1nSlYArpbCm9pTqVpnW8W8AM5444VxRlXOsYp3FdjOG-4MCA68cXyOvhx2hxSftz4X_RS3qZ9OaiYVMGC0URPFDtT0u5yT7_SQwsakUVPQb-X1obyeyut9ec0niR-kPMH90qf36f9YfwESRYga</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Ray, Sambit</creator><creator>Yadav, Jayprakash</creator><creator>Mishra, Amrita</creator><creator>Dasgupta, Sudip</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L6V</scope><scope>M7S</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><orcidid>https://orcid.org/0000-0002-9370-8862</orcidid></search><sort><creationdate>20230401</creationdate><title>In-vitro antimicrobial & biocompatibility study of Spherical 52S4.6 Submicron-Bioactive glass synthesized by Stöber Method: Effect of Ag Doping</title><author>Ray, Sambit ; Yadav, Jayprakash ; Mishra, Amrita ; Dasgupta, Sudip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-8b7d3297500c674ea6e179dcbd92e403238e4aca95cc253f50bfea3ca043038c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Assaying</topic><topic>Biocompatibility</topic><topic>Bioglass</topic><topic>Biological activity</topic><topic>Calcium nitrate</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Condensates</topic><topic>Doping</topic><topic>E coli</topic><topic>Effectiveness</topic><topic>Functional groups</topic><topic>Glass</topic><topic>Infrared spectroscopy</topic><topic>Inorganic Chemistry</topic><topic>Materials Science</topic><topic>Nanotechnology</topic><topic>Natural Materials</topic><topic>Nitrates</topic><topic>Optical and Electronic Materials</topic><topic>Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications</topic><topic>Regeneration (physiology)</topic><topic>Silver nitrate</topic><topic>Sodium nitrates</topic><topic>Soft tissues</topic><topic>Sol-gel processes</topic><topic>Spherical powders</topic><topic>Synthesis</topic><topic>Tetraethyl orthosilicate</topic><topic>Tissue engineering</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ray, Sambit</creatorcontrib><creatorcontrib>Yadav, Jayprakash</creatorcontrib><creatorcontrib>Mishra, Amrita</creatorcontrib><creatorcontrib>Dasgupta, Sudip</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Journal of sol-gel science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ray, Sambit</au><au>Yadav, Jayprakash</au><au>Mishra, Amrita</au><au>Dasgupta, Sudip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vitro antimicrobial & biocompatibility study of Spherical 52S4.6 Submicron-Bioactive glass synthesized by Stöber Method: Effect of Ag Doping</atitle><jtitle>Journal of sol-gel science and technology</jtitle><stitle>J Sol-Gel Sci Technol</stitle><date>2023-04-01</date><risdate>2023</risdate><volume>106</volume><issue>1</issue><spage>67</spage><epage>84</epage><pages>67-84</pages><issn>0928-0707</issn><eissn>1573-4846</eissn><abstract>Bioactive glass has become a promising material due to its biocompatibility and bioactivity toward both soft tissue and hard tissue regeneration. In the present study, nearly monodisperse, dense and spherical shaped un-doped and Ag-doped 52S4.6 submicron bioactive glass (SBG) particles were synthesized using sol-gel method with one-step base condensation i.e., Stöber method using tetraethyl orthosilicate (TEOS), calcium nitrate tetrahydrate (Ca(NO
3
)
2
•4H
2
O), triethyl phosphate (TEP), silver nitrate (AgNO
3
) and sodium nitrate (NaNO
3
) as precursors. The silver doping was performed at a concentration of 0.5 mole% and 1 mole% of total glass composition by changing the concentration of sodium nitrate in the bioactive glass network. The synthesized 52S4.6 SBG, both un-doped and Ag-doped powders showed predominately amorphous phase as suggested by the XRD data and indicated the presence of all the corresponding functional groups in the FT-IR spectra analysis. The synthesized powder exhibited spherical shape within the range of 448–671 nm as obtained from FE-SEM image. The synthesized 52S4.6 SBG elicited antimicrobial property against
Staphylococcus aureus
(
S. aureus
) and
Escherichia coli
(
E. coli
) at a concentration of 5 mg/ml and with an efficacy of 58.6% after 16 h incubation period. However, upon incorporation of Ag into the bioactive glass matrix efficacy in inhibiting the growth of
S. aureus
and
E. coli
was significantly enhanced to 99.6% and 99.2% with Minimum Inhibitory Concentration (MIC) of 10 mg/ml and 15 mg/ml, respectively at 0.5 mole% Ag-doped SBG at the same incubation interval. In-vitro cell culture study on biocompatibility and viability, namely live/dead assay and cell viability and proliferation assay such as MTT assay on MG-63 cell cultured Ag-doped and un-doped 52S4.6 SBG revealed that 0.5 mole% Ag-doped SBG exerted minimal cytotoxicity towards the cultured MG-63 cells.
Graphical Abstract
Highlights
Monodispersed, and spherical-shaped 52S4.6 bioactive glass (SBG) particles were synthesized.
0.5 mol% Ag-doped 52S4.6 SBG showed excellent bioactivity.
Minimum inhibitory concentrations for
E.coli
and
S. aureus
were 0.5 mole% Ag-doped 52S4.6 SBG at 10 mg/ml and 15 mg/ml, respectively.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s10971-023-06064-3</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-9370-8862</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0928-0707 |
| ispartof | Journal of sol-gel science and technology, 2023-04, Vol.106 (1), p.67-84 |
| issn | 0928-0707 1573-4846 |
| language | eng |
| recordid | cdi_proquest_journals_2790202189 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Antiinfectives and antibacterials Assaying Biocompatibility Bioglass Biological activity Calcium nitrate Ceramics Chemistry and Materials Science Composites Condensates Doping E coli Effectiveness Functional groups Glass Infrared spectroscopy Inorganic Chemistry Materials Science Nanotechnology Natural Materials Nitrates Optical and Electronic Materials Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications Regeneration (physiology) Silver nitrate Sodium nitrates Soft tissues Sol-gel processes Spherical powders Synthesis Tetraethyl orthosilicate Tissue engineering Toxicity |
| title | In-vitro antimicrobial & biocompatibility study of Spherical 52S4.6 Submicron-Bioactive glass synthesized by Stöber Method: Effect of Ag Doping |
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