Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India
The present study aims to assess the physicochemical properties and prevalence of microbial communities in soils samples collected from different locations of Cachar district, Assam, India. Bacterial communities in the soil were screened by morphological, biochemical and 16S rDNA sequence analysis a...
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| Veröffentlicht in: | SN applied sciences 2019-07, Vol.1 (7), p.727, Article 727 |
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| creator | Nath, Soumitra Paul, Payal Roy, Rajdeep Bhattacharjee, Satabdi Deb, Bibhas |
| description | The present study aims to assess the physicochemical properties and prevalence of microbial communities in soils samples collected from different locations of Cachar district, Assam, India. Bacterial communities in the soil were screened by morphological, biochemical and 16S rDNA sequence analysis and were identified as
Bacillus megaterium
,
Bacillus cereus
,
Pseudomonas aeruginosa
and
Chromobacterium pseudoviolaceum
. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Cd, Pb, Fe and Cu by agar dilution technique. Co-resistance of antibiotic was also determined, which demonstrated that most of the metal-tolerant isolates were resistant to Methicillin and Penicillin. However,
P. aeruginosa
showed resistance to other antibiotics such as Cefdinir, Ampicillin, Kanamycin, Rifampicin and Vancomycin. The development and evolution of antibiotic resistance in soil bacteria occurs very likely naturally as a result of unethical and non-scientific disposal of toxic substances and industrial discharge, which also includes heavy-metal effluents and other clinical by-products. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcare-associated infections. |
| doi_str_mv | 10.1007/s42452-019-0762-3 |
| format | Article |
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Bacillus megaterium
,
Bacillus cereus
,
Pseudomonas aeruginosa
and
Chromobacterium pseudoviolaceum
. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Cd, Pb, Fe and Cu by agar dilution technique. Co-resistance of antibiotic was also determined, which demonstrated that most of the metal-tolerant isolates were resistant to Methicillin and Penicillin. However,
P. aeruginosa
showed resistance to other antibiotics such as Cefdinir, Ampicillin, Kanamycin, Rifampicin and Vancomycin. The development and evolution of antibiotic resistance in soil bacteria occurs very likely naturally as a result of unethical and non-scientific disposal of toxic substances and industrial discharge, which also includes heavy-metal effluents and other clinical by-products. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcare-associated infections.</description><identifier>ISSN: 2523-3963</identifier><identifier>EISSN: 2523-3971</identifier><identifier>DOI: 10.1007/s42452-019-0762-3</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Ampicillin ; Antibiotic resistance ; Antibiotics ; Antimicrobial resistance ; Applied and Technical Physics ; Bacteria ; Cadmium ; Cefdinir ; Chemistry/Food Science ; Copper ; Dilution ; Drug resistance ; Earth and Environmental Sciences: Pollution and Health Impacts ; Earth Sciences ; Engineering ; Environment ; Genes ; Heavy metals ; Kanamycin ; Materials Science ; Metal concentrations ; Metals ; Methicillin ; Microbial activity ; Microorganisms ; Minimum inhibitory concentration ; Moisture content ; Organic chemicals ; PCB ; Penicillin ; Physicochemical properties ; Polychlorinated biphenyls ; Potassium ; Pseudomonas aeruginosa ; Pulp & paper industry ; Research Article ; Rifampin ; rRNA 16S ; Sequence analysis ; Soil bacteria ; Soil contamination ; Soil fertility ; Soil microorganisms ; Soil resistance ; Toxic substances ; Vancomycin</subject><ispartof>SN applied sciences, 2019-07, Vol.1 (7), p.727, Article 727</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>Springer Nature Switzerland AG 2019.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-2be24c51243bec10592dd44dca045cae4de2c8047a8d1e270f8fdb94fe38a70f3</citedby><cites>FETCH-LOGICAL-c359t-2be24c51243bec10592dd44dca045cae4de2c8047a8d1e270f8fdb94fe38a70f3</cites><orcidid>0000-0003-3678-2297</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Nath, Soumitra</creatorcontrib><creatorcontrib>Paul, Payal</creatorcontrib><creatorcontrib>Roy, Rajdeep</creatorcontrib><creatorcontrib>Bhattacharjee, Satabdi</creatorcontrib><creatorcontrib>Deb, Bibhas</creatorcontrib><title>Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India</title><title>SN applied sciences</title><addtitle>SN Appl. Sci</addtitle><description>The present study aims to assess the physicochemical properties and prevalence of microbial communities in soils samples collected from different locations of Cachar district, Assam, India. Bacterial communities in the soil were screened by morphological, biochemical and 16S rDNA sequence analysis and were identified as
Bacillus megaterium
,
Bacillus cereus
,
Pseudomonas aeruginosa
and
Chromobacterium pseudoviolaceum
. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Cd, Pb, Fe and Cu by agar dilution technique. Co-resistance of antibiotic was also determined, which demonstrated that most of the metal-tolerant isolates were resistant to Methicillin and Penicillin. However,
P. aeruginosa
showed resistance to other antibiotics such as Cefdinir, Ampicillin, Kanamycin, Rifampicin and Vancomycin. The development and evolution of antibiotic resistance in soil bacteria occurs very likely naturally as a result of unethical and non-scientific disposal of toxic substances and industrial discharge, which also includes heavy-metal effluents and other clinical by-products. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcare-associated infections.</description><subject>Ampicillin</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Applied and Technical Physics</subject><subject>Bacteria</subject><subject>Cadmium</subject><subject>Cefdinir</subject><subject>Chemistry/Food Science</subject><subject>Copper</subject><subject>Dilution</subject><subject>Drug resistance</subject><subject>Earth and Environmental Sciences: Pollution and Health Impacts</subject><subject>Earth Sciences</subject><subject>Engineering</subject><subject>Environment</subject><subject>Genes</subject><subject>Heavy metals</subject><subject>Kanamycin</subject><subject>Materials Science</subject><subject>Metal concentrations</subject><subject>Metals</subject><subject>Methicillin</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Minimum inhibitory concentration</subject><subject>Moisture content</subject><subject>Organic chemicals</subject><subject>PCB</subject><subject>Penicillin</subject><subject>Physicochemical properties</subject><subject>Polychlorinated biphenyls</subject><subject>Potassium</subject><subject>Pseudomonas aeruginosa</subject><subject>Pulp & paper industry</subject><subject>Research Article</subject><subject>Rifampin</subject><subject>rRNA 16S</subject><subject>Sequence analysis</subject><subject>Soil bacteria</subject><subject>Soil contamination</subject><subject>Soil fertility</subject><subject>Soil microorganisms</subject><subject>Soil resistance</subject><subject>Toxic substances</subject><subject>Vancomycin</subject><issn>2523-3963</issn><issn>2523-3971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKAzEUhoMoWGofwF3ArdFcOzPLUrwUCm50HTLJGU2ZTmqSLnwA39uMI7pydW7f_x_4Ebpk9IZRWt0myaXihLKG0GrJiThBM664IKKp2OlvvxTnaJHSjlLKq0bIWszQ5yaF3mQfBmwGh72DIfvO22kVOryHbHqSQw_RDPkbKtW3PmRvSYTkUx4PrbEZoje4i2GPU_A9TmZ_6CGNLmtj30zErsDR2zyuVqncr_FmcN5coLPO9AkWP3WOXu7vntePZPv0sFmvtsQK1WTCW-DSKsalaMEyqhrunJTOGiqVNSAdcFtTWZnaMeAV7erOtY3sQNSmTGKOribfQwzvR0hZ78IxDuWl5lVdS9kwpQrFJsrGkFKETh-i35v4oRnVY-B6ClyXwPUYuBZFwydNKuzwCvHP-X_RF4-IhXY</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Nath, Soumitra</creator><creator>Paul, Payal</creator><creator>Roy, Rajdeep</creator><creator>Bhattacharjee, Satabdi</creator><creator>Deb, Bibhas</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-3678-2297</orcidid></search><sort><creationdate>20190701</creationdate><title>Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India</title><author>Nath, Soumitra ; Paul, Payal ; Roy, Rajdeep ; Bhattacharjee, Satabdi ; Deb, Bibhas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-2be24c51243bec10592dd44dca045cae4de2c8047a8d1e270f8fdb94fe38a70f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Ampicillin</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Applied and Technical Physics</topic><topic>Bacteria</topic><topic>Cadmium</topic><topic>Cefdinir</topic><topic>Chemistry/Food Science</topic><topic>Copper</topic><topic>Dilution</topic><topic>Drug resistance</topic><topic>Earth and Environmental Sciences: Pollution and Health Impacts</topic><topic>Earth Sciences</topic><topic>Engineering</topic><topic>Environment</topic><topic>Genes</topic><topic>Heavy metals</topic><topic>Kanamycin</topic><topic>Materials Science</topic><topic>Metal concentrations</topic><topic>Metals</topic><topic>Methicillin</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Minimum inhibitory concentration</topic><topic>Moisture content</topic><topic>Organic chemicals</topic><topic>PCB</topic><topic>Penicillin</topic><topic>Physicochemical properties</topic><topic>Polychlorinated biphenyls</topic><topic>Potassium</topic><topic>Pseudomonas aeruginosa</topic><topic>Pulp & paper industry</topic><topic>Research Article</topic><topic>Rifampin</topic><topic>rRNA 16S</topic><topic>Sequence analysis</topic><topic>Soil bacteria</topic><topic>Soil contamination</topic><topic>Soil fertility</topic><topic>Soil microorganisms</topic><topic>Soil resistance</topic><topic>Toxic substances</topic><topic>Vancomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nath, Soumitra</creatorcontrib><creatorcontrib>Paul, Payal</creatorcontrib><creatorcontrib>Roy, Rajdeep</creatorcontrib><creatorcontrib>Bhattacharjee, Satabdi</creatorcontrib><creatorcontrib>Deb, Bibhas</creatorcontrib><collection>CrossRef</collection><jtitle>SN applied sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nath, Soumitra</au><au>Paul, Payal</au><au>Roy, Rajdeep</au><au>Bhattacharjee, Satabdi</au><au>Deb, Bibhas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India</atitle><jtitle>SN applied sciences</jtitle><stitle>SN Appl. Sci</stitle><date>2019-07-01</date><risdate>2019</risdate><volume>1</volume><issue>7</issue><spage>727</spage><pages>727-</pages><artnum>727</artnum><issn>2523-3963</issn><eissn>2523-3971</eissn><abstract>The present study aims to assess the physicochemical properties and prevalence of microbial communities in soils samples collected from different locations of Cachar district, Assam, India. Bacterial communities in the soil were screened by morphological, biochemical and 16S rDNA sequence analysis and were identified as
Bacillus megaterium
,
Bacillus cereus
,
Pseudomonas aeruginosa
and
Chromobacterium pseudoviolaceum
. High concentrations of toxic metals negatively affect bacterial growth, and therefore, the minimum inhibitory concentration of isolated bacteria was determined against Cd, Pb, Fe and Cu by agar dilution technique. Co-resistance of antibiotic was also determined, which demonstrated that most of the metal-tolerant isolates were resistant to Methicillin and Penicillin. However,
P. aeruginosa
showed resistance to other antibiotics such as Cefdinir, Ampicillin, Kanamycin, Rifampicin and Vancomycin. The development and evolution of antibiotic resistance in soil bacteria occurs very likely naturally as a result of unethical and non-scientific disposal of toxic substances and industrial discharge, which also includes heavy-metal effluents and other clinical by-products. Therefore, pragmatic measures must be taken to limit the spread of antimicrobial resistance across the environment and to reduce the incidence of healthcare-associated infections.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s42452-019-0762-3</doi><orcidid>https://orcid.org/0000-0003-3678-2297</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Ampicillin Antibiotic resistance Antibiotics Antimicrobial resistance Applied and Technical Physics Bacteria Cadmium Cefdinir Chemistry/Food Science Copper Dilution Drug resistance Earth and Environmental Sciences: Pollution and Health Impacts Earth Sciences Engineering Environment Genes Heavy metals Kanamycin Materials Science Metal concentrations Metals Methicillin Microbial activity Microorganisms Minimum inhibitory concentration Moisture content Organic chemicals PCB Penicillin Physicochemical properties Polychlorinated biphenyls Potassium Pseudomonas aeruginosa Pulp & paper industry Research Article Rifampin rRNA 16S Sequence analysis Soil bacteria Soil contamination Soil fertility Soil microorganisms Soil resistance Toxic substances Vancomycin |
| title | Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India |
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