Changes of cell adhesion and extracellular matrix (ECM) components in cervical intraepithelial neoplasia
Cell-cell and cell-extracellular matrix interaction is crucial in tumor progression. Tight junction (TJ) proteins as occludin and claudins (CLDNs) play important role in this process together with several extracellular matrix components, as syndecan. Our previous work suggested significant changes i...
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| creator | Sobel, Gábor Szabó, István Páska, Csilla Kiss, András Kovalszky, Ilona Kádár, Anna Paulin, Ferenc Schaff, Zsuzsa |
| description | Cell-cell and cell-extracellular matrix interaction is crucial in tumor progression. Tight junction (TJ) proteins as occludin and claudins (CLDNs) play important role in this process together with several extracellular matrix components, as syndecan. Our previous work suggested significant changes in the expression of claudins even in the early stages of cervical carcinogenesis. The aim of our present work was to study the expression of occludin and syndecan-1, as compared to CLDNs, in early phases of cervical carcinogenesis. Paraffin sections of 50 samples were studied by immunohistochemistry, including cervical intraepithelial neoplasias (CINI-II-III), in situ carcinomas (CIS) and normal cervical samples. Occludin and CLDN-2 were found colocalized in the basal layer, while syndecan-1 and CLDN-1, -4 and -7 were coexpressed in the parabasal and intermedier layers in normal epithelia. Intensity of occludin staining decreased in CIN/CIS lesions, although it was more extended towards the upper epithelial layers with inverse relation with grades, as seen in the case of CLDN-2 expression. CLDN-1, -2, -4 and -7 were detected in the entire epithelium in CIN, showing decrease in CIS. The progression of CIN was associated with reduced syndecan-1 expression, in contrast to CLDN-1, -4 and -7 which increased toward CIS. The obtained data suggest that significant changes occur in the composition of cell adhesion complexes even in early stages of cervical carcinogenesis. The pattern of expression is characteristic for the alteration, the changes in the different components, however, are not parallel with each other. |
| doi_str_mv | 10.1007/bf03032402 |
| format | Article |
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Tight junction (TJ) proteins as occludin and claudins (CLDNs) play important role in this process together with several extracellular matrix components, as syndecan. Our previous work suggested significant changes in the expression of claudins even in the early stages of cervical carcinogenesis. The aim of our present work was to study the expression of occludin and syndecan-1, as compared to CLDNs, in early phases of cervical carcinogenesis. Paraffin sections of 50 samples were studied by immunohistochemistry, including cervical intraepithelial neoplasias (CINI-II-III), in situ carcinomas (CIS) and normal cervical samples. Occludin and CLDN-2 were found colocalized in the basal layer, while syndecan-1 and CLDN-1, -4 and -7 were coexpressed in the parabasal and intermedier layers in normal epithelia. Intensity of occludin staining decreased in CIN/CIS lesions, although it was more extended towards the upper epithelial layers with inverse relation with grades, as seen in the case of CLDN-2 expression. CLDN-1, -2, -4 and -7 were detected in the entire epithelium in CIN, showing decrease in CIS. The progression of CIN was associated with reduced syndecan-1 expression, in contrast to CLDN-1, -4 and -7 which increased toward CIS. The obtained data suggest that significant changes occur in the composition of cell adhesion complexes even in early stages of cervical carcinogenesis. The pattern of expression is characteristic for the alteration, the changes in the different components, however, are not parallel with each other.</description><identifier>ISSN: 1219-4956</identifier><identifier>EISSN: 1532-2807</identifier><identifier>DOI: 10.1007/bf03032402</identifier><identifier>PMID: 15800679</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Biomarkers, Tumor - metabolism ; Carcinogenesis ; Carcinoma in Situ - metabolism ; Carcinoma in Situ - pathology ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Adhesion ; Cell adhesion & migration ; Cells ; Cervical carcinoma ; Cervical Intraepithelial Neoplasia - metabolism ; Cervical Intraepithelial Neoplasia - pathology ; Cervix Uteri - metabolism ; Cervix Uteri - pathology ; Claudin-1 ; Claudin-4 ; Claudins ; Epithelial Cells - metabolism ; Epithelium ; Extracellular matrix ; Extracellular Matrix - metabolism ; Extracellular Matrix - pathology ; Female ; Humans ; Immunoenzyme Techniques ; Immunohistochemistry ; Medical research ; Membrane Glycoproteins - metabolism ; Membrane Proteins - metabolism ; Neoplasm Staging ; Occludin ; Oncology ; Paraffin ; Pathology ; Proteoglycans - metabolism ; Syndecan ; Syndecan-1 ; Syndecans ; Uterine Cervical Neoplasms - metabolism ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Pathology oncology research, 2005-01, Vol.11 (1), p.26-31</ispartof><rights>Arányi Lajos Foundation 2005</rights><rights>Arányi Lajos Foundation 2005.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-74a184eb4bdf7de849f070fa573678153bdee5901517bf3b90d61aed54f5b2353</citedby><cites>FETCH-LOGICAL-c407t-74a184eb4bdf7de849f070fa573678153bdee5901517bf3b90d61aed54f5b2353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15800679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sobel, Gábor</creatorcontrib><creatorcontrib>Szabó, István</creatorcontrib><creatorcontrib>Páska, Csilla</creatorcontrib><creatorcontrib>Kiss, András</creatorcontrib><creatorcontrib>Kovalszky, Ilona</creatorcontrib><creatorcontrib>Kádár, Anna</creatorcontrib><creatorcontrib>Paulin, Ferenc</creatorcontrib><creatorcontrib>Schaff, Zsuzsa</creatorcontrib><title>Changes of cell adhesion and extracellular matrix (ECM) components in cervical intraepithelial neoplasia</title><title>Pathology oncology research</title><addtitle>Pathol Oncol Res</addtitle><description>Cell-cell and cell-extracellular matrix interaction is crucial in tumor progression. Tight junction (TJ) proteins as occludin and claudins (CLDNs) play important role in this process together with several extracellular matrix components, as syndecan. Our previous work suggested significant changes in the expression of claudins even in the early stages of cervical carcinogenesis. The aim of our present work was to study the expression of occludin and syndecan-1, as compared to CLDNs, in early phases of cervical carcinogenesis. Paraffin sections of 50 samples were studied by immunohistochemistry, including cervical intraepithelial neoplasias (CINI-II-III), in situ carcinomas (CIS) and normal cervical samples. Occludin and CLDN-2 were found colocalized in the basal layer, while syndecan-1 and CLDN-1, -4 and -7 were coexpressed in the parabasal and intermedier layers in normal epithelia. Intensity of occludin staining decreased in CIN/CIS lesions, although it was more extended towards the upper epithelial layers with inverse relation with grades, as seen in the case of CLDN-2 expression. CLDN-1, -2, -4 and -7 were detected in the entire epithelium in CIN, showing decrease in CIS. The progression of CIN was associated with reduced syndecan-1 expression, in contrast to CLDN-1, -4 and -7 which increased toward CIS. The obtained data suggest that significant changes occur in the composition of cell adhesion complexes even in early stages of cervical carcinogenesis. The pattern of expression is characteristic for the alteration, the changes in the different components, however, are not parallel with each other.</description><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinogenesis</subject><subject>Carcinoma in Situ - metabolism</subject><subject>Carcinoma in Situ - pathology</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cells</subject><subject>Cervical carcinoma</subject><subject>Cervical Intraepithelial Neoplasia - metabolism</subject><subject>Cervical Intraepithelial Neoplasia - pathology</subject><subject>Cervix Uteri - metabolism</subject><subject>Cervix Uteri - pathology</subject><subject>Claudin-1</subject><subject>Claudin-4</subject><subject>Claudins</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Medical research</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane Proteins - metabolism</subject><subject>Neoplasm Staging</subject><subject>Occludin</subject><subject>Oncology</subject><subject>Paraffin</subject><subject>Pathology</subject><subject>Proteoglycans - metabolism</subject><subject>Syndecan</subject><subject>Syndecan-1</subject><subject>Syndecans</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1219-4956</issn><issn>1532-2807</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1Lw0AQhhdRbP24-ANkwYsK0dmvbHLU0qpQ8aLnsElmbUqaxN1E6r93ayvePM07wzPvMC8hZwxuGIC-zS0IEFwC3yNjpgSPeAJ6P2jO0kimKh6RI--XEOA4jQ_JiKkEINbpmCwmC9O8o6etpQXWNTXlAn3VNtQ0JcV178xmPNTG0ZXpXbWml9PJ8xUt2lXXNtj0nlZNWHWfVWHqoMMGdlW_wLoKfYNtVxtfmRNyYE3t8XRXj8nbbPo6eYzmLw9Pk7t5VEjQfaSlYYnEXOal1SUmMrWgwRqlRayT8FxeIqoUmGI6tyJPoYyZwVJJq3IulDgmF1vfzrUfA_o-W7aDa8LJjOtE82DEkv8oxphIJIcf6npLFa713qHNOletjPvKGGSb6LP72W_0AT7fWQ75Css_dJe1-AbtAH1j</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Sobel, Gábor</creator><creator>Szabó, István</creator><creator>Páska, Csilla</creator><creator>Kiss, András</creator><creator>Kovalszky, Ilona</creator><creator>Kádár, Anna</creator><creator>Paulin, Ferenc</creator><creator>Schaff, Zsuzsa</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20050101</creationdate><title>Changes of cell adhesion and extracellular matrix (ECM) components in cervical intraepithelial neoplasia</title><author>Sobel, Gábor ; Szabó, István ; Páska, Csilla ; Kiss, András ; Kovalszky, Ilona ; Kádár, Anna ; Paulin, Ferenc ; Schaff, Zsuzsa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-74a184eb4bdf7de849f070fa573678153bdee5901517bf3b90d61aed54f5b2353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biomarkers, Tumor - 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Tight junction (TJ) proteins as occludin and claudins (CLDNs) play important role in this process together with several extracellular matrix components, as syndecan. Our previous work suggested significant changes in the expression of claudins even in the early stages of cervical carcinogenesis. The aim of our present work was to study the expression of occludin and syndecan-1, as compared to CLDNs, in early phases of cervical carcinogenesis. Paraffin sections of 50 samples were studied by immunohistochemistry, including cervical intraepithelial neoplasias (CINI-II-III), in situ carcinomas (CIS) and normal cervical samples. Occludin and CLDN-2 were found colocalized in the basal layer, while syndecan-1 and CLDN-1, -4 and -7 were coexpressed in the parabasal and intermedier layers in normal epithelia. Intensity of occludin staining decreased in CIN/CIS lesions, although it was more extended towards the upper epithelial layers with inverse relation with grades, as seen in the case of CLDN-2 expression. CLDN-1, -2, -4 and -7 were detected in the entire epithelium in CIN, showing decrease in CIS. The progression of CIN was associated with reduced syndecan-1 expression, in contrast to CLDN-1, -4 and -7 which increased toward CIS. The obtained data suggest that significant changes occur in the composition of cell adhesion complexes even in early stages of cervical carcinogenesis. The pattern of expression is characteristic for the alteration, the changes in the different components, however, are not parallel with each other.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15800679</pmid><doi>10.1007/bf03032402</doi><tpages>6</tpages></addata></record> |
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| subjects | Biomarkers, Tumor - metabolism Carcinogenesis Carcinoma in Situ - metabolism Carcinoma in Situ - pathology Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Adhesion Cell adhesion & migration Cells Cervical carcinoma Cervical Intraepithelial Neoplasia - metabolism Cervical Intraepithelial Neoplasia - pathology Cervix Uteri - metabolism Cervix Uteri - pathology Claudin-1 Claudin-4 Claudins Epithelial Cells - metabolism Epithelium Extracellular matrix Extracellular Matrix - metabolism Extracellular Matrix - pathology Female Humans Immunoenzyme Techniques Immunohistochemistry Medical research Membrane Glycoproteins - metabolism Membrane Proteins - metabolism Neoplasm Staging Occludin Oncology Paraffin Pathology Proteoglycans - metabolism Syndecan Syndecan-1 Syndecans Uterine Cervical Neoplasms - metabolism Uterine Cervical Neoplasms - pathology |
| title | Changes of cell adhesion and extracellular matrix (ECM) components in cervical intraepithelial neoplasia |
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