Production of human neurotrophin-3 in baculovirus expression system
Human NT-3 gene was inserted into pPAK9 and the resultant plasmid was used to co-transfect sf21 cells with Bsu36 I digested Bac6 genomic DNA. Recombinant baculovirus was isolated by plaque assay. Slot-hybridization, immunocytochemistry and serum-free culture of DRG demonstrated that NT-3 had been in...
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| Veröffentlicht in: | Chinese science bulletin 1998-09, Vol.43 (18), p.1562-1566 |
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| container_issue | 18 |
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| container_title | Chinese science bulletin |
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| creator | Mu, Yuanyue Liu, Hong Zhang, Jie Liu, Shuhong Wu, Yan Wang, Jiazheng Fan, Ming |
| description | Human NT-3 gene was inserted into pPAK9 and the resultant plasmid was used to co-transfect sf21 cells with Bsu36 I digested Bac6 genomic DNA. Recombinant baculovirus was isolated by plaque assay. Slot-hybridization, immunocytochemistry and serum-free culture of DRG demonstrated that NT-3 had been integrated into Bac6 DNA, expressed and secreted. The yield of purified hNT-3 was 200 μg from 200 mL cultural supernatant after passing CM Sepharose Fast Flow column, the concentration for its maximum bioactivity was 4 ng/mL. |
| doi_str_mv | 10.1007/BF02883449 |
| format | Article |
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Recombinant baculovirus was isolated by plaque assay. Slot-hybridization, immunocytochemistry and serum-free culture of DRG demonstrated that NT-3 had been integrated into Bac6 DNA, expressed and secreted. 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| fulltext | fulltext |
| identifier | ISSN: 1001-6538 |
| ispartof | Chinese science bulletin, 1998-09, Vol.43 (18), p.1562-1566 |
| issn | 1001-6538 2095-9273 1861-9541 2095-9281 |
| language | eng |
| recordid | cdi_proquest_journals_2786916112 |
| source | Alma/SFX Local Collection |
| subjects | Baculovirus Biological activity Cell culture Deoxyribonucleic acid DNA expression Gene expression Hybridization Immunocytochemistry Neurotrophin 3 neurotrophin3 Plaque assay |
| title | Production of human neurotrophin-3 in baculovirus expression system |
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