Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration
Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the ro...
Gespeichert in:
| Veröffentlicht in: | Iranian polymer journal 2023-04, Vol.32 (4), p.469-481 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 481 |
|---|---|
| container_issue | 4 |
| container_start_page | 469 |
| container_title | Iranian polymer journal |
| container_volume | 32 |
| creator | Navarro-Cerón, Aurora Barceló-Santana, Federico Humberto Vera-Graziano, Ricardo Rivera-Torres, Filiberto Jiménez-Ávila, Alberto Rosales-Ibáñez, Raúl Navarro-Cerón, Elizabeth Castell-Rodríguez, Andrés Eliu Maciel-Cerda, Alfredo |
| description | Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the roots of teeth from a 2-year-old cattle. The dentin collagen was characterized by energy dispersive X-ray spectroscopy (EDS), and carbon, nitrogen, and oxygen were found to be the main elements of the protein. Infrared analysis revealed the typical bands of collagen at about 3300, 1631, 1539, and 1234 cm
−1
for amides A, I, II, and III, respectively. Calorimetric and infrared analyses also demonstrated that the collagen was non-denatured. With scanning electron microscopy, it was found that the thinnest fibers with a diameter comparable to that of fibers in the extracellular matrix were obtained when dentin collagen and acetic acid (AAc) were added to the solution of PLA in trifluoroethanol (TFE). The scaffolds with the thinnest diameter had also the highest porosity, and we considered that they could be beneficial in the growth of dentin cell. Human placenta-derived mesenchymal stem cells were seeded onto electrospun scaffolds. After 24, 48 and 96 h of culture, cell proliferation was evaluated by two independent strategies. In both assays, it was found that the pl-MSCs were capable of adhering and proliferating in different scaffolds. It was also observed that cell adhesion and proliferation increased significantly in scaffolds containing collagen, although the addition of AAc slightly decreased this effect on all scaffolds.
Graphical abstract |
| doi_str_mv | 10.1007/s13726-023-01139-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2784402681</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2784402681</sourcerecordid><originalsourceid>FETCH-LOGICAL-c244t-a94ee62ccda69cb3407417a1834df1537d0019d91717629e7c42f7438bb145b83</originalsourceid><addsrcrecordid>eNp9kE9LxDAQxYMouKz7BTwFvOihbiZJm_aoi_9gQQ96Dmk6XSO1WZOssN_eaAVvnubBvPeG-RFyCuwSGFPLCELxqmBcFAxANMX-gMxAibIoeVUeZs3yGrI-JosYXctYKUUly3pGnq79pxuRdjgmN1Lrh8FscFxu_bA_H4xNzlJjXXdBozV974cu0t4HmhDTK00uxh3SgDmCwSTnxxNy1Jsh4uJ3zsnL7c3z6r5YP949rK7WheVSpsI0ErHi1namamwrJFMSlIFayK6HUqiOMWi6BhSoijeorOS9kqJuW5BlW4s5OZt6t8F_7DAm_eZ3YcwnNVe1lPnlGrKLTy4bfIwBe70N7t2EvQamv-HpCZ7O8PQPPL3PITGFYjaPGwx_1f-kvgBcJ3Hp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2784402681</pqid></control><display><type>article</type><title>Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration</title><source>SpringerNature Journals</source><creator>Navarro-Cerón, Aurora ; Barceló-Santana, Federico Humberto ; Vera-Graziano, Ricardo ; Rivera-Torres, Filiberto ; Jiménez-Ávila, Alberto ; Rosales-Ibáñez, Raúl ; Navarro-Cerón, Elizabeth ; Castell-Rodríguez, Andrés Eliu ; Maciel-Cerda, Alfredo</creator><creatorcontrib>Navarro-Cerón, Aurora ; Barceló-Santana, Federico Humberto ; Vera-Graziano, Ricardo ; Rivera-Torres, Filiberto ; Jiménez-Ávila, Alberto ; Rosales-Ibáñez, Raúl ; Navarro-Cerón, Elizabeth ; Castell-Rodríguez, Andrés Eliu ; Maciel-Cerda, Alfredo</creatorcontrib><description>Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the roots of teeth from a 2-year-old cattle. The dentin collagen was characterized by energy dispersive X-ray spectroscopy (EDS), and carbon, nitrogen, and oxygen were found to be the main elements of the protein. Infrared analysis revealed the typical bands of collagen at about 3300, 1631, 1539, and 1234 cm
−1
for amides A, I, II, and III, respectively. Calorimetric and infrared analyses also demonstrated that the collagen was non-denatured. With scanning electron microscopy, it was found that the thinnest fibers with a diameter comparable to that of fibers in the extracellular matrix were obtained when dentin collagen and acetic acid (AAc) were added to the solution of PLA in trifluoroethanol (TFE). The scaffolds with the thinnest diameter had also the highest porosity, and we considered that they could be beneficial in the growth of dentin cell. Human placenta-derived mesenchymal stem cells were seeded onto electrospun scaffolds. After 24, 48 and 96 h of culture, cell proliferation was evaluated by two independent strategies. In both assays, it was found that the pl-MSCs were capable of adhering and proliferating in different scaffolds. It was also observed that cell adhesion and proliferation increased significantly in scaffolds containing collagen, although the addition of AAc slightly decreased this effect on all scaffolds.
Graphical abstract</description><identifier>ISSN: 1026-1265</identifier><identifier>EISSN: 1735-5265</identifier><identifier>DOI: 10.1007/s13726-023-01139-y</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetic acid ; Acids ; Amides ; Cattle ; Cell adhesion ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Collagen ; Composites ; Dentin ; Extracellular matrix ; Fibers ; Glass ; Hydroxyapatite ; Infrared analysis ; Natural Materials ; Original Research ; Polylactic acid ; Polymer Sciences ; Regeneration (physiology) ; Scaffolds ; Stem cells ; Tissue engineering</subject><ispartof>Iranian polymer journal, 2023-04, Vol.32 (4), p.469-481</ispartof><rights>The Author(s) 2023</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c244t-a94ee62ccda69cb3407417a1834df1537d0019d91717629e7c42f7438bb145b83</cites><orcidid>0000-0003-2881-2759 ; 0000-0001-5014-5206 ; 0000-0001-7468-5178 ; 0000-0002-8294-7032 ; 0000-0003-0714-4421 ; 0000-0002-3990-3720 ; 0000-0002-2713-8603 ; 0000-0003-2870-9831 ; 0000-0002-6674-1480</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13726-023-01139-y$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13726-023-01139-y$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Navarro-Cerón, Aurora</creatorcontrib><creatorcontrib>Barceló-Santana, Federico Humberto</creatorcontrib><creatorcontrib>Vera-Graziano, Ricardo</creatorcontrib><creatorcontrib>Rivera-Torres, Filiberto</creatorcontrib><creatorcontrib>Jiménez-Ávila, Alberto</creatorcontrib><creatorcontrib>Rosales-Ibáñez, Raúl</creatorcontrib><creatorcontrib>Navarro-Cerón, Elizabeth</creatorcontrib><creatorcontrib>Castell-Rodríguez, Andrés Eliu</creatorcontrib><creatorcontrib>Maciel-Cerda, Alfredo</creatorcontrib><title>Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration</title><title>Iranian polymer journal</title><addtitle>Iran Polym J</addtitle><description>Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the roots of teeth from a 2-year-old cattle. The dentin collagen was characterized by energy dispersive X-ray spectroscopy (EDS), and carbon, nitrogen, and oxygen were found to be the main elements of the protein. Infrared analysis revealed the typical bands of collagen at about 3300, 1631, 1539, and 1234 cm
−1
for amides A, I, II, and III, respectively. Calorimetric and infrared analyses also demonstrated that the collagen was non-denatured. With scanning electron microscopy, it was found that the thinnest fibers with a diameter comparable to that of fibers in the extracellular matrix were obtained when dentin collagen and acetic acid (AAc) were added to the solution of PLA in trifluoroethanol (TFE). The scaffolds with the thinnest diameter had also the highest porosity, and we considered that they could be beneficial in the growth of dentin cell. Human placenta-derived mesenchymal stem cells were seeded onto electrospun scaffolds. After 24, 48 and 96 h of culture, cell proliferation was evaluated by two independent strategies. In both assays, it was found that the pl-MSCs were capable of adhering and proliferating in different scaffolds. It was also observed that cell adhesion and proliferation increased significantly in scaffolds containing collagen, although the addition of AAc slightly decreased this effect on all scaffolds.
Graphical abstract</description><subject>Acetic acid</subject><subject>Acids</subject><subject>Amides</subject><subject>Cattle</subject><subject>Cell adhesion</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Collagen</subject><subject>Composites</subject><subject>Dentin</subject><subject>Extracellular matrix</subject><subject>Fibers</subject><subject>Glass</subject><subject>Hydroxyapatite</subject><subject>Infrared analysis</subject><subject>Natural Materials</subject><subject>Original Research</subject><subject>Polylactic acid</subject><subject>Polymer Sciences</subject><subject>Regeneration (physiology)</subject><subject>Scaffolds</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>1026-1265</issn><issn>1735-5265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kE9LxDAQxYMouKz7BTwFvOihbiZJm_aoi_9gQQ96Dmk6XSO1WZOssN_eaAVvnubBvPeG-RFyCuwSGFPLCELxqmBcFAxANMX-gMxAibIoeVUeZs3yGrI-JosYXctYKUUly3pGnq79pxuRdjgmN1Lrh8FscFxu_bA_H4xNzlJjXXdBozV974cu0t4HmhDTK00uxh3SgDmCwSTnxxNy1Jsh4uJ3zsnL7c3z6r5YP949rK7WheVSpsI0ErHi1namamwrJFMSlIFayK6HUqiOMWi6BhSoijeorOS9kqJuW5BlW4s5OZt6t8F_7DAm_eZ3YcwnNVe1lPnlGrKLTy4bfIwBe70N7t2EvQamv-HpCZ7O8PQPPL3PITGFYjaPGwx_1f-kvgBcJ3Hp</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Navarro-Cerón, Aurora</creator><creator>Barceló-Santana, Federico Humberto</creator><creator>Vera-Graziano, Ricardo</creator><creator>Rivera-Torres, Filiberto</creator><creator>Jiménez-Ávila, Alberto</creator><creator>Rosales-Ibáñez, Raúl</creator><creator>Navarro-Cerón, Elizabeth</creator><creator>Castell-Rodríguez, Andrés Eliu</creator><creator>Maciel-Cerda, Alfredo</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-2881-2759</orcidid><orcidid>https://orcid.org/0000-0001-5014-5206</orcidid><orcidid>https://orcid.org/0000-0001-7468-5178</orcidid><orcidid>https://orcid.org/0000-0002-8294-7032</orcidid><orcidid>https://orcid.org/0000-0003-0714-4421</orcidid><orcidid>https://orcid.org/0000-0002-3990-3720</orcidid><orcidid>https://orcid.org/0000-0002-2713-8603</orcidid><orcidid>https://orcid.org/0000-0003-2870-9831</orcidid><orcidid>https://orcid.org/0000-0002-6674-1480</orcidid></search><sort><creationdate>20230401</creationdate><title>Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration</title><author>Navarro-Cerón, Aurora ; Barceló-Santana, Federico Humberto ; Vera-Graziano, Ricardo ; Rivera-Torres, Filiberto ; Jiménez-Ávila, Alberto ; Rosales-Ibáñez, Raúl ; Navarro-Cerón, Elizabeth ; Castell-Rodríguez, Andrés Eliu ; Maciel-Cerda, Alfredo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c244t-a94ee62ccda69cb3407417a1834df1537d0019d91717629e7c42f7438bb145b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acetic acid</topic><topic>Acids</topic><topic>Amides</topic><topic>Cattle</topic><topic>Cell adhesion</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Collagen</topic><topic>Composites</topic><topic>Dentin</topic><topic>Extracellular matrix</topic><topic>Fibers</topic><topic>Glass</topic><topic>Hydroxyapatite</topic><topic>Infrared analysis</topic><topic>Natural Materials</topic><topic>Original Research</topic><topic>Polylactic acid</topic><topic>Polymer Sciences</topic><topic>Regeneration (physiology)</topic><topic>Scaffolds</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Navarro-Cerón, Aurora</creatorcontrib><creatorcontrib>Barceló-Santana, Federico Humberto</creatorcontrib><creatorcontrib>Vera-Graziano, Ricardo</creatorcontrib><creatorcontrib>Rivera-Torres, Filiberto</creatorcontrib><creatorcontrib>Jiménez-Ávila, Alberto</creatorcontrib><creatorcontrib>Rosales-Ibáñez, Raúl</creatorcontrib><creatorcontrib>Navarro-Cerón, Elizabeth</creatorcontrib><creatorcontrib>Castell-Rodríguez, Andrés Eliu</creatorcontrib><creatorcontrib>Maciel-Cerda, Alfredo</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><jtitle>Iranian polymer journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Navarro-Cerón, Aurora</au><au>Barceló-Santana, Federico Humberto</au><au>Vera-Graziano, Ricardo</au><au>Rivera-Torres, Filiberto</au><au>Jiménez-Ávila, Alberto</au><au>Rosales-Ibáñez, Raúl</au><au>Navarro-Cerón, Elizabeth</au><au>Castell-Rodríguez, Andrés Eliu</au><au>Maciel-Cerda, Alfredo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration</atitle><jtitle>Iranian polymer journal</jtitle><stitle>Iran Polym J</stitle><date>2023-04-01</date><risdate>2023</risdate><volume>32</volume><issue>4</issue><spage>469</spage><epage>481</epage><pages>469-481</pages><issn>1026-1265</issn><eissn>1735-5265</eissn><abstract>Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the roots of teeth from a 2-year-old cattle. The dentin collagen was characterized by energy dispersive X-ray spectroscopy (EDS), and carbon, nitrogen, and oxygen were found to be the main elements of the protein. Infrared analysis revealed the typical bands of collagen at about 3300, 1631, 1539, and 1234 cm
−1
for amides A, I, II, and III, respectively. Calorimetric and infrared analyses also demonstrated that the collagen was non-denatured. With scanning electron microscopy, it was found that the thinnest fibers with a diameter comparable to that of fibers in the extracellular matrix were obtained when dentin collagen and acetic acid (AAc) were added to the solution of PLA in trifluoroethanol (TFE). The scaffolds with the thinnest diameter had also the highest porosity, and we considered that they could be beneficial in the growth of dentin cell. Human placenta-derived mesenchymal stem cells were seeded onto electrospun scaffolds. After 24, 48 and 96 h of culture, cell proliferation was evaluated by two independent strategies. In both assays, it was found that the pl-MSCs were capable of adhering and proliferating in different scaffolds. It was also observed that cell adhesion and proliferation increased significantly in scaffolds containing collagen, although the addition of AAc slightly decreased this effect on all scaffolds.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s13726-023-01139-y</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-2881-2759</orcidid><orcidid>https://orcid.org/0000-0001-5014-5206</orcidid><orcidid>https://orcid.org/0000-0001-7468-5178</orcidid><orcidid>https://orcid.org/0000-0002-8294-7032</orcidid><orcidid>https://orcid.org/0000-0003-0714-4421</orcidid><orcidid>https://orcid.org/0000-0002-3990-3720</orcidid><orcidid>https://orcid.org/0000-0002-2713-8603</orcidid><orcidid>https://orcid.org/0000-0003-2870-9831</orcidid><orcidid>https://orcid.org/0000-0002-6674-1480</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1026-1265 |
| ispartof | Iranian polymer journal, 2023-04, Vol.32 (4), p.469-481 |
| issn | 1026-1265 1735-5265 |
| language | eng |
| recordid | cdi_proquest_journals_2784402681 |
| source | SpringerNature Journals |
| subjects | Acetic acid Acids Amides Cattle Cell adhesion Ceramics Chemistry Chemistry and Materials Science Collagen Composites Dentin Extracellular matrix Fibers Glass Hydroxyapatite Infrared analysis Natural Materials Original Research Polylactic acid Polymer Sciences Regeneration (physiology) Scaffolds Stem cells Tissue engineering |
| title | Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T17%3A21%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bovine%20dentin%20collagen/poly(lactic%20acid)%20scaffolds%20for%20teeth%20tissue%20regeneration&rft.jtitle=Iranian%20polymer%20journal&rft.au=Navarro-Cer%C3%B3n,%20Aurora&rft.date=2023-04-01&rft.volume=32&rft.issue=4&rft.spage=469&rft.epage=481&rft.pages=469-481&rft.issn=1026-1265&rft.eissn=1735-5265&rft_id=info:doi/10.1007/s13726-023-01139-y&rft_dat=%3Cproquest_cross%3E2784402681%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2784402681&rft_id=info:pmid/&rfr_iscdi=true |