A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP
Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsyna...
Gespeichert in:
| Veröffentlicht in: | Biophysics (Oxford) 2022-12, Vol.67 (6), p.1007-1010 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1010 |
|---|---|
| container_issue | 6 |
| container_start_page | 1007 |
| container_title | Biophysics (Oxford) |
| container_volume | 67 |
| creator | Khairullin, A. E. Grishin, S. N. Teplov, A. Yu Eremeev, A. A. Baltina, T. V. Ziganshin, A. U. |
| description | Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In
in vitro
experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca
2+
content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia. |
| doi_str_mv | 10.1134/S0006350922050086 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2782225861</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2782225861</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1836-f2bfe7cb09fdad386300d7b7b6289e5d1fd71771d908fbfd43a8bff0eb4efc073</originalsourceid><addsrcrecordid>eNp1kEtLw0AUhQdRsFZ_gLsB19E7k8wjy1qqLUQsWNch87g1JU3qTCr035tSwYW4uovzfefCIeSWwT1jafbwBgAyFZBzDgJAyzMyYkKIREqRnZPRMU6O-SW5inEDwDLIxIgUEzqv1x90WjW23m9p4b98kzxW0Tv60jnfUOwCXTjf9jUe6nZNl13s46Gtdn1t6QzR2z7SDulktbwmF1g10d_83DF5f5qtpvOkeH1eTCdFYplOZYLcoFfWQI6ucqmWKYBTRhnJde6FY-gUU4q5HDQadFlaaYMI3mQeLah0TO5OvbvQfe597MtNtw_t8LLkSnPOhZZsoNiJsqGLMXgsd6HeVuFQMiiPo5V_RhscfnLiwLZrH36b_5e-AXPrbQM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2782225861</pqid></control><display><type>article</type><title>A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP</title><source>SpringerLink Journals - AutoHoldings</source><creator>Khairullin, A. E. ; Grishin, S. N. ; Teplov, A. Yu ; Eremeev, A. A. ; Baltina, T. V. ; Ziganshin, A. U.</creator><creatorcontrib>Khairullin, A. E. ; Grishin, S. N. ; Teplov, A. Yu ; Eremeev, A. A. ; Baltina, T. V. ; Ziganshin, A. U.</creatorcontrib><description>Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In
in vitro
experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca
2+
content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia.</description><identifier>ISSN: 0006-3509</identifier><identifier>EISSN: 1555-6654</identifier><identifier>DOI: 10.1134/S0006350922050086</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biological and Medical Physics ; Biophysics ; Calcium (intracellular) ; Carbachol ; Complex Systems Biophysics ; Electrical stimuli ; Electromyography ; Hypothermia ; Muscle contraction ; Physics ; Physics and Astronomy ; Skeletal muscle ; Synaptic transmission</subject><ispartof>Biophysics (Oxford), 2022-12, Vol.67 (6), p.1007-1010</ispartof><rights>Pleiades Publishing, Inc. 2022. ISSN 0006-3509, Biophysics, 2022, Vol. 67, No. 6, pp. 1007–1010. © Pleiades Publishing, Inc., 2022. Russian Text © The Author(s), 2022, published in Biofizika, 2022, Vol. 67, No. 6, pp. 1232–1235.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1836-f2bfe7cb09fdad386300d7b7b6289e5d1fd71771d908fbfd43a8bff0eb4efc073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006350922050086$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006350922050086$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Khairullin, A. E.</creatorcontrib><creatorcontrib>Grishin, S. N.</creatorcontrib><creatorcontrib>Teplov, A. Yu</creatorcontrib><creatorcontrib>Eremeev, A. A.</creatorcontrib><creatorcontrib>Baltina, T. V.</creatorcontrib><creatorcontrib>Ziganshin, A. U.</creatorcontrib><title>A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP</title><title>Biophysics (Oxford)</title><addtitle>BIOPHYSICS</addtitle><description>Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In
in vitro
experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca
2+
content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia.</description><subject>Biological and Medical Physics</subject><subject>Biophysics</subject><subject>Calcium (intracellular)</subject><subject>Carbachol</subject><subject>Complex Systems Biophysics</subject><subject>Electrical stimuli</subject><subject>Electromyography</subject><subject>Hypothermia</subject><subject>Muscle contraction</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Skeletal muscle</subject><subject>Synaptic transmission</subject><issn>0006-3509</issn><issn>1555-6654</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLw0AUhQdRsFZ_gLsB19E7k8wjy1qqLUQsWNch87g1JU3qTCr035tSwYW4uovzfefCIeSWwT1jafbwBgAyFZBzDgJAyzMyYkKIREqRnZPRMU6O-SW5inEDwDLIxIgUEzqv1x90WjW23m9p4b98kzxW0Tv60jnfUOwCXTjf9jUe6nZNl13s46Gtdn1t6QzR2z7SDulktbwmF1g10d_83DF5f5qtpvOkeH1eTCdFYplOZYLcoFfWQI6ucqmWKYBTRhnJde6FY-gUU4q5HDQadFlaaYMI3mQeLah0TO5OvbvQfe597MtNtw_t8LLkSnPOhZZsoNiJsqGLMXgsd6HeVuFQMiiPo5V_RhscfnLiwLZrH36b_5e-AXPrbQM</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Khairullin, A. E.</creator><creator>Grishin, S. N.</creator><creator>Teplov, A. Yu</creator><creator>Eremeev, A. A.</creator><creator>Baltina, T. V.</creator><creator>Ziganshin, A. U.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20221201</creationdate><title>A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP</title><author>Khairullin, A. E. ; Grishin, S. N. ; Teplov, A. Yu ; Eremeev, A. A. ; Baltina, T. V. ; Ziganshin, A. U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1836-f2bfe7cb09fdad386300d7b7b6289e5d1fd71771d908fbfd43a8bff0eb4efc073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological and Medical Physics</topic><topic>Biophysics</topic><topic>Calcium (intracellular)</topic><topic>Carbachol</topic><topic>Complex Systems Biophysics</topic><topic>Electrical stimuli</topic><topic>Electromyography</topic><topic>Hypothermia</topic><topic>Muscle contraction</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><topic>Skeletal muscle</topic><topic>Synaptic transmission</topic><toplevel>online_resources</toplevel><creatorcontrib>Khairullin, A. E.</creatorcontrib><creatorcontrib>Grishin, S. N.</creatorcontrib><creatorcontrib>Teplov, A. Yu</creatorcontrib><creatorcontrib>Eremeev, A. A.</creatorcontrib><creatorcontrib>Baltina, T. V.</creatorcontrib><creatorcontrib>Ziganshin, A. U.</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biophysics (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khairullin, A. E.</au><au>Grishin, S. N.</au><au>Teplov, A. Yu</au><au>Eremeev, A. A.</au><au>Baltina, T. V.</au><au>Ziganshin, A. U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP</atitle><jtitle>Biophysics (Oxford)</jtitle><stitle>BIOPHYSICS</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>67</volume><issue>6</issue><spage>1007</spage><epage>1010</epage><pages>1007-1010</pages><issn>0006-3509</issn><eissn>1555-6654</eissn><abstract>Identification of the pre- and postsynaptic effects of ATP is a methodological challenge. In our previous study, the role of P2 receptor signaling in synaptic transmission processes was evaluated using carbachol-induced skeletal muscle contractions. The search for models that can record the postsynaptic side of purinergic signaling during the application of electrical stimulation led to the idea of controlling the presynaptic terminal of ATP-mediated modulation. In
in vitro
experiments, electromyograms and mechanomyograms during isometric contractions of isolated nerve-muscle preparations of rat soleus and extensor digitorum longus (EDL) muscles revealed postsynaptic effects of ATP in the presence of a high intracellular calcium level. Thus, the effects of ATP in the presence of increased Ca
2+
content were seen through contraction of soleus muscles that started to contract quicker by fifty percent and inhibition of contractility of EDL muscles; this was in accord with data obtained earlier on carbachol-induced contractions. We have demonstrated an ATP-dependent processes in the postsynaptic site that may contribute significantly to adaptation mechanisms in hypothermia.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0006350922050086</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3509 |
| ispartof | Biophysics (Oxford), 2022-12, Vol.67 (6), p.1007-1010 |
| issn | 0006-3509 1555-6654 |
| language | eng |
| recordid | cdi_proquest_journals_2782225861 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biological and Medical Physics Biophysics Calcium (intracellular) Carbachol Complex Systems Biophysics Electrical stimuli Electromyography Hypothermia Muscle contraction Physics Physics and Astronomy Skeletal muscle Synaptic transmission |
| title | A High Calcium Level-Based Model for Identifying Postsynaptic Effects of ATP |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A28%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20High%20Calcium%20Level-Based%20Model%20for%20Identifying%20Postsynaptic%20Effects%20of%20ATP&rft.jtitle=Biophysics%20(Oxford)&rft.au=Khairullin,%20A.%20E.&rft.date=2022-12-01&rft.volume=67&rft.issue=6&rft.spage=1007&rft.epage=1010&rft.pages=1007-1010&rft.issn=0006-3509&rft.eissn=1555-6654&rft_id=info:doi/10.1134/S0006350922050086&rft_dat=%3Cproquest_cross%3E2782225861%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2782225861&rft_id=info:pmid/&rfr_iscdi=true |