Maize Methionine Sulfoxide Reductase Genes ZmMSRA2 and ZmMSRA5.1 Involved in the Tolerance to Osmotic or Salinity Stress in Arabidopsis and Maize
Methionine (Met), including the free and protein-bound forms, can easily be oxidized in the presence of excess reactive oxygen species (ROS) under conditions of abiotic stress to form Met sulfoxide (MetO). This reaction can be reversed by MetO reductases (MSRs), which are known to be involved in a v...
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| description | Methionine (Met), including the free and protein-bound forms, can easily be oxidized in the presence of excess reactive oxygen species (ROS) under conditions of abiotic stress to form Met sulfoxide (MetO). This reaction can be reversed by MetO reductases (MSRs), which are known to be involved in a variety of stress response mechanisms in plants, but the functions of their maize MSRA homologs have not been reported to date. Cytoplasmic
ZmMSRA2
and secretary
ZmMSRA5.1
, previously shown to be induced under salinity or drought stress, belong to the
MSRA
subfamily. In the present study, their constitutive expression in
Arabidopsis
resulted in notable increases in MSR enzymatic activity; virus-induced gene silencing (VIGS) analysis in maize indicated that the silencing expression of
ZmMSRA2
or
ZmMSRA5.1
decreased the tolerance of seedlings to osmotic or salinity, respectively; the ectopic expression of
ZmMSRA2
in
Arabidopsis
increased the tolerance of seedlings to mannitol, H
2
O
2
, and ABA, and that of
ZmMSRA5.1
enhanced the tolerance to NaCl and H
2
O
2
. Compared with that of the wild-type, the germination rates of seeds overexpressing
ZmMSRA2
or
ZmMSRA5.1
were higher under osmotic or salinity stress, respectively. The effect of active
ZmMSRA2
in
Arabidopsis
was the suppression of ROS accumulation and the increase in intracellular proline content;
ZmMSRA5.1
led to improved ion transport and decreased ROS content. The expression of the maize MSRA homologs in
Arabidopsis
resulted in no observed changes in the transcription of
Arabidopsis MSRA
subfamily members, while genes of the
MSRB
subfamily were downregulated overall. Together, our findings suggest that
ZmMSRA2
participates in osmotic stress tolerance by decreasing the ROS content, and enhancing the proline and ABA pathways, whereas
ZmMSRA5.1
is involved in tolerance to salinity by reducing ROS accumulation and modifying ion transportation. Characterization of these two genes contributes to the understanding of redox metabolism in maize. |
| doi_str_mv | 10.1007/s11105-022-01354-6 |
| format | Article |
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ZmMSRA2
and secretary
ZmMSRA5.1
, previously shown to be induced under salinity or drought stress, belong to the
MSRA
subfamily. In the present study, their constitutive expression in
Arabidopsis
resulted in notable increases in MSR enzymatic activity; virus-induced gene silencing (VIGS) analysis in maize indicated that the silencing expression of
ZmMSRA2
or
ZmMSRA5.1
decreased the tolerance of seedlings to osmotic or salinity, respectively; the ectopic expression of
ZmMSRA2
in
Arabidopsis
increased the tolerance of seedlings to mannitol, H
2
O
2
, and ABA, and that of
ZmMSRA5.1
enhanced the tolerance to NaCl and H
2
O
2
. Compared with that of the wild-type, the germination rates of seeds overexpressing
ZmMSRA2
or
ZmMSRA5.1
were higher under osmotic or salinity stress, respectively. The effect of active
ZmMSRA2
in
Arabidopsis
was the suppression of ROS accumulation and the increase in intracellular proline content;
ZmMSRA5.1
led to improved ion transport and decreased ROS content. The expression of the maize MSRA homologs in
Arabidopsis
resulted in no observed changes in the transcription of
Arabidopsis MSRA
subfamily members, while genes of the
MSRB
subfamily were downregulated overall. Together, our findings suggest that
ZmMSRA2
participates in osmotic stress tolerance by decreasing the ROS content, and enhancing the proline and ABA pathways, whereas
ZmMSRA5.1
is involved in tolerance to salinity by reducing ROS accumulation and modifying ion transportation. Characterization of these two genes contributes to the understanding of redox metabolism in maize.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-022-01354-6</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Abscisic acid ; Accumulation ; Arabidopsis ; Bioinformatics ; Biomedical and Life Sciences ; Corn ; Drought ; Ectopic expression ; Enzymatic activity ; Gene silencing ; Genes ; Germination ; Homology ; Hydrogen peroxide ; Ion transport ; Life Sciences ; Mannitol ; Metabolomics ; Methionine ; Original Article ; Osmotic stress ; Plant Breeding/Biotechnology ; Plant Sciences ; Proline ; Proteomics ; Reactive oxygen species ; Reductases ; Salinity ; Salinity effects ; Seedlings ; Seeds ; Sodium chloride ; Sulfoxides</subject><ispartof>Plant molecular biology reporter, 2023-03, Vol.41 (1), p.118-133</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-17616e1358f376b6af6db5c843e12a647cbf37df7ea43c4b59611388a5869f183</citedby><cites>FETCH-LOGICAL-c319t-17616e1358f376b6af6db5c843e12a647cbf37df7ea43c4b59611388a5869f183</cites><orcidid>0000-0002-3557-9388</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11105-022-01354-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11105-022-01354-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids></links><search><creatorcontrib>Hu, Dandan</creatorcontrib><creatorcontrib>Guo, Qiang</creatorcontrib><creatorcontrib>Zhang, Yuhua</creatorcontrib><creatorcontrib>Chen, Fanguo</creatorcontrib><title>Maize Methionine Sulfoxide Reductase Genes ZmMSRA2 and ZmMSRA5.1 Involved in the Tolerance to Osmotic or Salinity Stress in Arabidopsis and Maize</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>Methionine (Met), including the free and protein-bound forms, can easily be oxidized in the presence of excess reactive oxygen species (ROS) under conditions of abiotic stress to form Met sulfoxide (MetO). This reaction can be reversed by MetO reductases (MSRs), which are known to be involved in a variety of stress response mechanisms in plants, but the functions of their maize MSRA homologs have not been reported to date. Cytoplasmic
ZmMSRA2
and secretary
ZmMSRA5.1
, previously shown to be induced under salinity or drought stress, belong to the
MSRA
subfamily. In the present study, their constitutive expression in
Arabidopsis
resulted in notable increases in MSR enzymatic activity; virus-induced gene silencing (VIGS) analysis in maize indicated that the silencing expression of
ZmMSRA2
or
ZmMSRA5.1
decreased the tolerance of seedlings to osmotic or salinity, respectively; the ectopic expression of
ZmMSRA2
in
Arabidopsis
increased the tolerance of seedlings to mannitol, H
2
O
2
, and ABA, and that of
ZmMSRA5.1
enhanced the tolerance to NaCl and H
2
O
2
. Compared with that of the wild-type, the germination rates of seeds overexpressing
ZmMSRA2
or
ZmMSRA5.1
were higher under osmotic or salinity stress, respectively. The effect of active
ZmMSRA2
in
Arabidopsis
was the suppression of ROS accumulation and the increase in intracellular proline content;
ZmMSRA5.1
led to improved ion transport and decreased ROS content. The expression of the maize MSRA homologs in
Arabidopsis
resulted in no observed changes in the transcription of
Arabidopsis MSRA
subfamily members, while genes of the
MSRB
subfamily were downregulated overall. Together, our findings suggest that
ZmMSRA2
participates in osmotic stress tolerance by decreasing the ROS content, and enhancing the proline and ABA pathways, whereas
ZmMSRA5.1
is involved in tolerance to salinity by reducing ROS accumulation and modifying ion transportation. Characterization of these two genes contributes to the understanding of redox metabolism in maize.</description><subject>Abscisic acid</subject><subject>Accumulation</subject><subject>Arabidopsis</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Corn</subject><subject>Drought</subject><subject>Ectopic expression</subject><subject>Enzymatic activity</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Germination</subject><subject>Homology</subject><subject>Hydrogen peroxide</subject><subject>Ion transport</subject><subject>Life Sciences</subject><subject>Mannitol</subject><subject>Metabolomics</subject><subject>Methionine</subject><subject>Original Article</subject><subject>Osmotic stress</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Proline</subject><subject>Proteomics</subject><subject>Reactive oxygen species</subject><subject>Reductases</subject><subject>Salinity</subject><subject>Salinity effects</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Sodium chloride</subject><subject>Sulfoxides</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtOwzAQRS0EEuXxA6wssQ544thxllXFS2qF1MKGjeUkEzBK7WK7CPgL_pi0RWLHakaac-_MXELOgF0AY-VlBAAmMpbnGQMuikzukRGIMs8qBWqfjFjJRVbJgh2Soxhf2SBiSo3I98zYL6QzTC_WO-uQLtZ95z9si3SO7bpJJiK9QYeRPi1ni_k4p8a1v724AHrn3n3_ji21jqYXpA--x2BcgzR5eh-XPtmG-kAXprfOpk-6SAFj3ODjYGrb-lW0cWu6veWEHHSmj3j6W4_J4_XVw-Q2m97f3E3G06zhUKUMSgkSh19Vx0tZS9PJthaNKjhCbmRRNvUwaLsSTcGbohaVBOBKGaFk1YHix-R857sK_m2NMelXvw5uWKnzUjGhihyqgcp3VBN8jAE7vQp2acKnBqY30etd9HqIXm-j13IQ8Z0oDrB7xvBn_Y_qBysahjs</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Hu, Dandan</creator><creator>Guo, Qiang</creator><creator>Zhang, Yuhua</creator><creator>Chen, Fanguo</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-3557-9388</orcidid></search><sort><creationdate>20230301</creationdate><title>Maize Methionine Sulfoxide Reductase Genes ZmMSRA2 and ZmMSRA5.1 Involved in the Tolerance to Osmotic or Salinity Stress in Arabidopsis and Maize</title><author>Hu, Dandan ; Guo, Qiang ; Zhang, Yuhua ; Chen, Fanguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-17616e1358f376b6af6db5c843e12a647cbf37df7ea43c4b59611388a5869f183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abscisic acid</topic><topic>Accumulation</topic><topic>Arabidopsis</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Corn</topic><topic>Drought</topic><topic>Ectopic expression</topic><topic>Enzymatic activity</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Germination</topic><topic>Homology</topic><topic>Hydrogen peroxide</topic><topic>Ion transport</topic><topic>Life Sciences</topic><topic>Mannitol</topic><topic>Metabolomics</topic><topic>Methionine</topic><topic>Original Article</topic><topic>Osmotic stress</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Proline</topic><topic>Proteomics</topic><topic>Reactive oxygen species</topic><topic>Reductases</topic><topic>Salinity</topic><topic>Salinity effects</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Sodium chloride</topic><topic>Sulfoxides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Dandan</creatorcontrib><creatorcontrib>Guo, Qiang</creatorcontrib><creatorcontrib>Zhang, Yuhua</creatorcontrib><creatorcontrib>Chen, Fanguo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Dandan</au><au>Guo, Qiang</au><au>Zhang, Yuhua</au><au>Chen, Fanguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maize Methionine Sulfoxide Reductase Genes ZmMSRA2 and ZmMSRA5.1 Involved in the Tolerance to Osmotic or Salinity Stress in Arabidopsis and Maize</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2023-03-01</date><risdate>2023</risdate><volume>41</volume><issue>1</issue><spage>118</spage><epage>133</epage><pages>118-133</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Methionine (Met), including the free and protein-bound forms, can easily be oxidized in the presence of excess reactive oxygen species (ROS) under conditions of abiotic stress to form Met sulfoxide (MetO). This reaction can be reversed by MetO reductases (MSRs), which are known to be involved in a variety of stress response mechanisms in plants, but the functions of their maize MSRA homologs have not been reported to date. Cytoplasmic
ZmMSRA2
and secretary
ZmMSRA5.1
, previously shown to be induced under salinity or drought stress, belong to the
MSRA
subfamily. In the present study, their constitutive expression in
Arabidopsis
resulted in notable increases in MSR enzymatic activity; virus-induced gene silencing (VIGS) analysis in maize indicated that the silencing expression of
ZmMSRA2
or
ZmMSRA5.1
decreased the tolerance of seedlings to osmotic or salinity, respectively; the ectopic expression of
ZmMSRA2
in
Arabidopsis
increased the tolerance of seedlings to mannitol, H
2
O
2
, and ABA, and that of
ZmMSRA5.1
enhanced the tolerance to NaCl and H
2
O
2
. Compared with that of the wild-type, the germination rates of seeds overexpressing
ZmMSRA2
or
ZmMSRA5.1
were higher under osmotic or salinity stress, respectively. The effect of active
ZmMSRA2
in
Arabidopsis
was the suppression of ROS accumulation and the increase in intracellular proline content;
ZmMSRA5.1
led to improved ion transport and decreased ROS content. The expression of the maize MSRA homologs in
Arabidopsis
resulted in no observed changes in the transcription of
Arabidopsis MSRA
subfamily members, while genes of the
MSRB
subfamily were downregulated overall. Together, our findings suggest that
ZmMSRA2
participates in osmotic stress tolerance by decreasing the ROS content, and enhancing the proline and ABA pathways, whereas
ZmMSRA5.1
is involved in tolerance to salinity by reducing ROS accumulation and modifying ion transportation. Characterization of these two genes contributes to the understanding of redox metabolism in maize.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11105-022-01354-6</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-3557-9388</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Plant molecular biology reporter, 2023-03, Vol.41 (1), p.118-133 |
| issn | 0735-9640 1572-9818 |
| language | eng |
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| source | Springer Online Journals Complete |
| subjects | Abscisic acid Accumulation Arabidopsis Bioinformatics Biomedical and Life Sciences Corn Drought Ectopic expression Enzymatic activity Gene silencing Genes Germination Homology Hydrogen peroxide Ion transport Life Sciences Mannitol Metabolomics Methionine Original Article Osmotic stress Plant Breeding/Biotechnology Plant Sciences Proline Proteomics Reactive oxygen species Reductases Salinity Salinity effects Seedlings Seeds Sodium chloride Sulfoxides |
| title | Maize Methionine Sulfoxide Reductase Genes ZmMSRA2 and ZmMSRA5.1 Involved in the Tolerance to Osmotic or Salinity Stress in Arabidopsis and Maize |
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