Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica
Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon ca...
Gespeichert in:
| Veröffentlicht in: | Processes 2023-02, Vol.11 (2), p.301 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | 301 |
| container_title | Processes |
| container_volume | 11 |
| creator | Alduraihem, Nuha Suliman Bhat, Ramesa Shafi Al-Zahrani, Sabah Ahmed Elnagar, Doaa M. Alobaid, Hussah M. Daghestani, Maha H. |
| description | Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression levels of eight genes (β-catenin, APC, TP53, Beclin1, DKK3, Axin, Cyclin D1, and C-myc) were checked by a reverse transcription-polymerase chain reaction in cancer cells before and after treatment with A. nilotica extract and A. nilotica-AgNPs. Prepared nanoparticles were characterized through ultraviolet-visible (UV-vis), Zetasizer, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to identify the functional group in extracts. At first, AgNPs were confirmed by a sharp peak of surface plasmon resonance at 375 nm. The Z-average size was 105.4 nm with a polydispersity index of 0.297. TEM showed particle size of 11–30 nm. The prepared AgNPs showed promising antibacterial activity against bacterial strains and cytotoxic activity against the cancer cell lines. Expression levels of all the genes were affected by extract and AgNPs treatment. Overall, this study recommended both A. nilotica pods and A. nilotica-AgNPs as attractive candidates for antibacterial and anticancer applications. |
| doi_str_mv | 10.3390/pr11020301 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2779667457</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2779667457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c295t-be691975f03f8e2a6cf9fc3d315af41723b722da1bcd9c77a75efb3a89892a1a3</originalsourceid><addsrcrecordid>eNpNkFtLxDAQhYMouOi--AsCvgnVXLad5rEs3mBRYfW5TNMEs3STmnQX1l9vywo6LzMD35zDHEKuOLuVUrG7PnLOBJOMn5CZEAIyBRxO_83nZJ7Sho2luCzzYkZs5Qen0WsTKfqWTuvW6Rgahx2t9OD2bjjQYOnadfsRekEfeozjUWcSXR_88GmS-zYttTFs6Vto00RXGrVD6l0XJv1LcmaxS2b-2y_Ix8P9-_IpW70-Pi-rVaaFyoesMYXiCnLLpC2NwEJbZbVsJc_RLjgI2YAQLfJGt0oDIOTGNhJLVSqBHOUFuT7q9jF87Uwa6k3YRT9a1gJAFQUschipmyM1_plSNLbuo9tiPNSc1VOU9V-U8gcROWc8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2779667457</pqid></control><display><type>article</type><title>Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><creator>Alduraihem, Nuha Suliman ; Bhat, Ramesa Shafi ; Al-Zahrani, Sabah Ahmed ; Elnagar, Doaa M. ; Alobaid, Hussah M. ; Daghestani, Maha H.</creator><creatorcontrib>Alduraihem, Nuha Suliman ; Bhat, Ramesa Shafi ; Al-Zahrani, Sabah Ahmed ; Elnagar, Doaa M. ; Alobaid, Hussah M. ; Daghestani, Maha H.</creatorcontrib><description>Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression levels of eight genes (β-catenin, APC, TP53, Beclin1, DKK3, Axin, Cyclin D1, and C-myc) were checked by a reverse transcription-polymerase chain reaction in cancer cells before and after treatment with A. nilotica extract and A. nilotica-AgNPs. Prepared nanoparticles were characterized through ultraviolet-visible (UV-vis), Zetasizer, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to identify the functional group in extracts. At first, AgNPs were confirmed by a sharp peak of surface plasmon resonance at 375 nm. The Z-average size was 105.4 nm with a polydispersity index of 0.297. TEM showed particle size of 11–30 nm. The prepared AgNPs showed promising antibacterial activity against bacterial strains and cytotoxic activity against the cancer cell lines. Expression levels of all the genes were affected by extract and AgNPs treatment. Overall, this study recommended both A. nilotica pods and A. nilotica-AgNPs as attractive candidates for antibacterial and anticancer applications.</description><identifier>ISSN: 2227-9717</identifier><identifier>EISSN: 2227-9717</identifier><identifier>DOI: 10.3390/pr11020301</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Acacia nilotica ; Adenomatous polyposis coli ; Amino acids ; Antibacterial activity ; Antibiotic resistance ; Antibiotics ; Anticancer properties ; Antimicrobial activity ; Antimicrobial agents ; Bacteria ; c-Myc protein ; Colon cancer ; Colorectal cancer ; Cyclin D1 ; Cytotoxicity ; Fourier transforms ; Functional groups ; Gene expression ; Genes ; Infrared spectroscopy ; Myc protein ; Nanoparticles ; p53 Protein ; Pneumonia ; Polydispersity ; Polymerase chain reaction ; Proteins ; Reverse transcription ; Scanning electron microscopy ; Silver ; Spectrum analysis ; Strains (organisms) ; Surface plasmon resonance ; Synthesis ; Transmission electron microscopy ; Tumor cell lines ; Tumors ; β-Catenin</subject><ispartof>Processes, 2023-02, Vol.11 (2), p.301</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-be691975f03f8e2a6cf9fc3d315af41723b722da1bcd9c77a75efb3a89892a1a3</citedby><cites>FETCH-LOGICAL-c295t-be691975f03f8e2a6cf9fc3d315af41723b722da1bcd9c77a75efb3a89892a1a3</cites><orcidid>0000-0001-6478-1238 ; 0000-0002-6735-8758 ; 0000-0003-2149-6239</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Alduraihem, Nuha Suliman</creatorcontrib><creatorcontrib>Bhat, Ramesa Shafi</creatorcontrib><creatorcontrib>Al-Zahrani, Sabah Ahmed</creatorcontrib><creatorcontrib>Elnagar, Doaa M.</creatorcontrib><creatorcontrib>Alobaid, Hussah M.</creatorcontrib><creatorcontrib>Daghestani, Maha H.</creatorcontrib><title>Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica</title><title>Processes</title><description>Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression levels of eight genes (β-catenin, APC, TP53, Beclin1, DKK3, Axin, Cyclin D1, and C-myc) were checked by a reverse transcription-polymerase chain reaction in cancer cells before and after treatment with A. nilotica extract and A. nilotica-AgNPs. Prepared nanoparticles were characterized through ultraviolet-visible (UV-vis), Zetasizer, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to identify the functional group in extracts. At first, AgNPs were confirmed by a sharp peak of surface plasmon resonance at 375 nm. The Z-average size was 105.4 nm with a polydispersity index of 0.297. TEM showed particle size of 11–30 nm. The prepared AgNPs showed promising antibacterial activity against bacterial strains and cytotoxic activity against the cancer cell lines. Expression levels of all the genes were affected by extract and AgNPs treatment. Overall, this study recommended both A. nilotica pods and A. nilotica-AgNPs as attractive candidates for antibacterial and anticancer applications.</description><subject>Acacia nilotica</subject><subject>Adenomatous polyposis coli</subject><subject>Amino acids</subject><subject>Antibacterial activity</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Anticancer properties</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>c-Myc protein</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Cyclin D1</subject><subject>Cytotoxicity</subject><subject>Fourier transforms</subject><subject>Functional groups</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Infrared spectroscopy</subject><subject>Myc protein</subject><subject>Nanoparticles</subject><subject>p53 Protein</subject><subject>Pneumonia</subject><subject>Polydispersity</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Reverse transcription</subject><subject>Scanning electron microscopy</subject><subject>Silver</subject><subject>Spectrum analysis</subject><subject>Strains (organisms)</subject><subject>Surface plasmon resonance</subject><subject>Synthesis</subject><subject>Transmission electron microscopy</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>β-Catenin</subject><issn>2227-9717</issn><issn>2227-9717</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNkFtLxDAQhYMouOi--AsCvgnVXLad5rEs3mBRYfW5TNMEs3STmnQX1l9vywo6LzMD35zDHEKuOLuVUrG7PnLOBJOMn5CZEAIyBRxO_83nZJ7Sho2luCzzYkZs5Qen0WsTKfqWTuvW6Rgahx2t9OD2bjjQYOnadfsRekEfeozjUWcSXR_88GmS-zYttTFs6Vto00RXGrVD6l0XJv1LcmaxS2b-2y_Ix8P9-_IpW70-Pi-rVaaFyoesMYXiCnLLpC2NwEJbZbVsJc_RLjgI2YAQLfJGt0oDIOTGNhJLVSqBHOUFuT7q9jF87Uwa6k3YRT9a1gJAFQUschipmyM1_plSNLbuo9tiPNSc1VOU9V-U8gcROWc8</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Alduraihem, Nuha Suliman</creator><creator>Bhat, Ramesa Shafi</creator><creator>Al-Zahrani, Sabah Ahmed</creator><creator>Elnagar, Doaa M.</creator><creator>Alobaid, Hussah M.</creator><creator>Daghestani, Maha H.</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>LK8</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-6478-1238</orcidid><orcidid>https://orcid.org/0000-0002-6735-8758</orcidid><orcidid>https://orcid.org/0000-0003-2149-6239</orcidid></search><sort><creationdate>20230201</creationdate><title>Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica</title><author>Alduraihem, Nuha Suliman ; Bhat, Ramesa Shafi ; Al-Zahrani, Sabah Ahmed ; Elnagar, Doaa M. ; Alobaid, Hussah M. ; Daghestani, Maha H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-be691975f03f8e2a6cf9fc3d315af41723b722da1bcd9c77a75efb3a89892a1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acacia nilotica</topic><topic>Adenomatous polyposis coli</topic><topic>Amino acids</topic><topic>Antibacterial activity</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Anticancer properties</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>c-Myc protein</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Cyclin D1</topic><topic>Cytotoxicity</topic><topic>Fourier transforms</topic><topic>Functional groups</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Infrared spectroscopy</topic><topic>Myc protein</topic><topic>Nanoparticles</topic><topic>p53 Protein</topic><topic>Pneumonia</topic><topic>Polydispersity</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Reverse transcription</topic><topic>Scanning electron microscopy</topic><topic>Silver</topic><topic>Spectrum analysis</topic><topic>Strains (organisms)</topic><topic>Surface plasmon resonance</topic><topic>Synthesis</topic><topic>Transmission electron microscopy</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alduraihem, Nuha Suliman</creatorcontrib><creatorcontrib>Bhat, Ramesa Shafi</creatorcontrib><creatorcontrib>Al-Zahrani, Sabah Ahmed</creatorcontrib><creatorcontrib>Elnagar, Doaa M.</creatorcontrib><creatorcontrib>Alobaid, Hussah M.</creatorcontrib><creatorcontrib>Daghestani, Maha H.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Processes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alduraihem, Nuha Suliman</au><au>Bhat, Ramesa Shafi</au><au>Al-Zahrani, Sabah Ahmed</au><au>Elnagar, Doaa M.</au><au>Alobaid, Hussah M.</au><au>Daghestani, Maha H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica</atitle><jtitle>Processes</jtitle><date>2023-02-01</date><risdate>2023</risdate><volume>11</volume><issue>2</issue><spage>301</spage><pages>301-</pages><issn>2227-9717</issn><eissn>2227-9717</eissn><abstract>Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression levels of eight genes (β-catenin, APC, TP53, Beclin1, DKK3, Axin, Cyclin D1, and C-myc) were checked by a reverse transcription-polymerase chain reaction in cancer cells before and after treatment with A. nilotica extract and A. nilotica-AgNPs. Prepared nanoparticles were characterized through ultraviolet-visible (UV-vis), Zetasizer, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to identify the functional group in extracts. At first, AgNPs were confirmed by a sharp peak of surface plasmon resonance at 375 nm. The Z-average size was 105.4 nm with a polydispersity index of 0.297. TEM showed particle size of 11–30 nm. The prepared AgNPs showed promising antibacterial activity against bacterial strains and cytotoxic activity against the cancer cell lines. Expression levels of all the genes were affected by extract and AgNPs treatment. Overall, this study recommended both A. nilotica pods and A. nilotica-AgNPs as attractive candidates for antibacterial and anticancer applications.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/pr11020301</doi><orcidid>https://orcid.org/0000-0001-6478-1238</orcidid><orcidid>https://orcid.org/0000-0002-6735-8758</orcidid><orcidid>https://orcid.org/0000-0003-2149-6239</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2227-9717 |
| ispartof | Processes, 2023-02, Vol.11 (2), p.301 |
| issn | 2227-9717 2227-9717 |
| language | eng |
| recordid | cdi_proquest_journals_2779667457 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute |
| subjects | Acacia nilotica Adenomatous polyposis coli Amino acids Antibacterial activity Antibiotic resistance Antibiotics Anticancer properties Antimicrobial activity Antimicrobial agents Bacteria c-Myc protein Colon cancer Colorectal cancer Cyclin D1 Cytotoxicity Fourier transforms Functional groups Gene expression Genes Infrared spectroscopy Myc protein Nanoparticles p53 Protein Pneumonia Polydispersity Polymerase chain reaction Proteins Reverse transcription Scanning electron microscopy Silver Spectrum analysis Strains (organisms) Surface plasmon resonance Synthesis Transmission electron microscopy Tumor cell lines Tumors β-Catenin |
| title | Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T17%3A31%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anticancer%20and%20Antimicrobial%20Activity%20of%20Silver%20Nanoparticles%20Synthesized%20from%20Pods%20of%20Acacia%20nilotica&rft.jtitle=Processes&rft.au=Alduraihem,%20Nuha%20Suliman&rft.date=2023-02-01&rft.volume=11&rft.issue=2&rft.spage=301&rft.pages=301-&rft.issn=2227-9717&rft.eissn=2227-9717&rft_id=info:doi/10.3390/pr11020301&rft_dat=%3Cproquest_cross%3E2779667457%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2779667457&rft_id=info:pmid/&rfr_iscdi=true |