Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement
This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe 3 O 4 ) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe 3 O 4 . The synthesized material αCS@Fe 3 O 4 wa...
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| Veröffentlicht in: | Cellulose (London) 2023-03, Vol.30 (4), p.2385-2398 |
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| creator | Lee, Seung-Cheol Lee, Seung-Ho Kang, Da-Hyun Kim, Min Sung, Jung-Suk Kadam, Avinash A. |
| description | This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe
3
O
4
) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe
3
O
4
. The synthesized material αCS@Fe
3
O
4
was structurally and morphologically characterized by SEM, TEM, XRD, TGA, and XPS analyses. The characterizations confirmed a nanostructural modification of αCS using Fe
3
O
4
nanoparticles. Next, the fabricated material αCS@Fe
3
O
4
was assessed for biocompatibility. The obtained data confirmed the biocompatible nature of the αCS@Fe
3
O
4
. Then, the αCS@Fe
3
O
4
was applied for the osteoconductive differentiation of the hADSCs. The hADSCs osteoconduction was enhanced significantly (11.325 fold increase) in the presence of the αCS@Fe
3
O
4
compared to the control hADSCs. The Alizarin Red S (ARS) staining microscopic images corroborated the osteoconduction enhancement. Furthermore, the relative gene expression of the important osteogenic markers ALP, OCN, and RUNX2 was analyzed. The expression levels were significantly enhanced in the presence of the target material αCS@Fe
3
O
4
. Finally, the immunofluorescent staining of ALP, OCN, and RUNX2 corroborated the enhanced osteoconduction. Thus, in conclusion, αCS@Fe
3
O
4
is a low-cost, economical, biocompatible nano-biomaterial with significant osteoconduction enhancement potential that can be applied in bone defect treatments in the future. |
| doi_str_mv | 10.1007/s10570-023-05045-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2778478404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2778478404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c270t-c18e640b8114f4822568a00b5c748feec72759352d9fb9d4676d73e3be2aac673</originalsourceid><addsrcrecordid>eNp9kM9KxDAQxoMouK6-gKeC5-gkTZr2KIv_QPCggreQptPdrm1Sk1bwsXwRn8nWFbwJAzPDfN838CPklME5A1AXkYFUQIGnFCQISdUeWTCpOM1z_rJPFlBkxXwuDslRjFsAKBRnC_L6OPYYOrN2ODQ2-fqkFtt2bH2cNmecp9GauvZtFZPah2QzdsYlpmp6H5FWGJp3rJI4YJfMxpj4afbWu2q0Q-Ndgm5jnMUO3XBMDmrTRjz57UvyfH31tLql9w83d6vLe2q5goFalmMmoMwZE7XIOZdZbgBKaZXIa0SruJJFKnlV1GVRiUxllUoxLZEbYzOVLsnZLrcP_m3EOOitH4ObXmquVC6mAjGp-E5lg48xYK370HQmfGgGeoaqd1D1RE3_QNVzdLozxUns1hj-ov9xfQMys3zG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2778478404</pqid></control><display><type>article</type><title>Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement</title><source>SpringerLink Journals - AutoHoldings</source><creator>Lee, Seung-Cheol ; Lee, Seung-Ho ; Kang, Da-Hyun ; Kim, Min ; Sung, Jung-Suk ; Kadam, Avinash A.</creator><creatorcontrib>Lee, Seung-Cheol ; Lee, Seung-Ho ; Kang, Da-Hyun ; Kim, Min ; Sung, Jung-Suk ; Kadam, Avinash A.</creatorcontrib><description>This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe
3
O
4
) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe
3
O
4
. The synthesized material αCS@Fe
3
O
4
was structurally and morphologically characterized by SEM, TEM, XRD, TGA, and XPS analyses. The characterizations confirmed a nanostructural modification of αCS using Fe
3
O
4
nanoparticles. Next, the fabricated material αCS@Fe
3
O
4
was assessed for biocompatibility. The obtained data confirmed the biocompatible nature of the αCS@Fe
3
O
4
. Then, the αCS@Fe
3
O
4
was applied for the osteoconductive differentiation of the hADSCs. The hADSCs osteoconduction was enhanced significantly (11.325 fold increase) in the presence of the αCS@Fe
3
O
4
compared to the control hADSCs. The Alizarin Red S (ARS) staining microscopic images corroborated the osteoconduction enhancement. Furthermore, the relative gene expression of the important osteogenic markers ALP, OCN, and RUNX2 was analyzed. The expression levels were significantly enhanced in the presence of the target material αCS@Fe
3
O
4
. Finally, the immunofluorescent staining of ALP, OCN, and RUNX2 corroborated the enhanced osteoconduction. Thus, in conclusion, αCS@Fe
3
O
4
is a low-cost, economical, biocompatible nano-biomaterial with significant osteoconduction enhancement potential that can be applied in bone defect treatments in the future.</description><identifier>ISSN: 0969-0239</identifier><identifier>EISSN: 1572-882X</identifier><identifier>DOI: 10.1007/s10570-023-05045-7</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alizarin ; Biocompatibility ; Biomedical materials ; Bioorganic Chemistry ; Cellulose ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Composites ; Gene expression ; Glass ; Image enhancement ; Iron oxides ; Nanomaterials ; Nanoparticles ; Natural Materials ; Organic Chemistry ; Original Research ; Physical Chemistry ; Polymer Sciences ; Staining ; Stem cells ; Sustainable Development ; Synthesis ; X ray photoelectron spectroscopy</subject><ispartof>Cellulose (London), 2023-03, Vol.30 (4), p.2385-2398</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-c18e640b8114f4822568a00b5c748feec72759352d9fb9d4676d73e3be2aac673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10570-023-05045-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10570-023-05045-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Lee, Seung-Cheol</creatorcontrib><creatorcontrib>Lee, Seung-Ho</creatorcontrib><creatorcontrib>Kang, Da-Hyun</creatorcontrib><creatorcontrib>Kim, Min</creatorcontrib><creatorcontrib>Sung, Jung-Suk</creatorcontrib><creatorcontrib>Kadam, Avinash A.</creatorcontrib><title>Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement</title><title>Cellulose (London)</title><addtitle>Cellulose</addtitle><description>This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe
3
O
4
) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe
3
O
4
. The synthesized material αCS@Fe
3
O
4
was structurally and morphologically characterized by SEM, TEM, XRD, TGA, and XPS analyses. The characterizations confirmed a nanostructural modification of αCS using Fe
3
O
4
nanoparticles. Next, the fabricated material αCS@Fe
3
O
4
was assessed for biocompatibility. The obtained data confirmed the biocompatible nature of the αCS@Fe
3
O
4
. Then, the αCS@Fe
3
O
4
was applied for the osteoconductive differentiation of the hADSCs. The hADSCs osteoconduction was enhanced significantly (11.325 fold increase) in the presence of the αCS@Fe
3
O
4
compared to the control hADSCs. The Alizarin Red S (ARS) staining microscopic images corroborated the osteoconduction enhancement. Furthermore, the relative gene expression of the important osteogenic markers ALP, OCN, and RUNX2 was analyzed. The expression levels were significantly enhanced in the presence of the target material αCS@Fe
3
O
4
. Finally, the immunofluorescent staining of ALP, OCN, and RUNX2 corroborated the enhanced osteoconduction. Thus, in conclusion, αCS@Fe
3
O
4
is a low-cost, economical, biocompatible nano-biomaterial with significant osteoconduction enhancement potential that can be applied in bone defect treatments in the future.</description><subject>Alizarin</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bioorganic Chemistry</subject><subject>Cellulose</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Gene expression</subject><subject>Glass</subject><subject>Image enhancement</subject><subject>Iron oxides</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Natural Materials</subject><subject>Organic Chemistry</subject><subject>Original Research</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Staining</subject><subject>Stem cells</subject><subject>Sustainable Development</subject><subject>Synthesis</subject><subject>X ray photoelectron spectroscopy</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kM9KxDAQxoMouK6-gKeC5-gkTZr2KIv_QPCggreQptPdrm1Sk1bwsXwRn8nWFbwJAzPDfN838CPklME5A1AXkYFUQIGnFCQISdUeWTCpOM1z_rJPFlBkxXwuDslRjFsAKBRnC_L6OPYYOrN2ODQ2-fqkFtt2bH2cNmecp9GauvZtFZPah2QzdsYlpmp6H5FWGJp3rJI4YJfMxpj4afbWu2q0Q-Ndgm5jnMUO3XBMDmrTRjz57UvyfH31tLql9w83d6vLe2q5goFalmMmoMwZE7XIOZdZbgBKaZXIa0SruJJFKnlV1GVRiUxllUoxLZEbYzOVLsnZLrcP_m3EOOitH4ObXmquVC6mAjGp-E5lg48xYK370HQmfGgGeoaqd1D1RE3_QNVzdLozxUns1hj-ov9xfQMys3zG</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Lee, Seung-Cheol</creator><creator>Lee, Seung-Ho</creator><creator>Kang, Da-Hyun</creator><creator>Kim, Min</creator><creator>Sung, Jung-Suk</creator><creator>Kadam, Avinash A.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20230301</creationdate><title>Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement</title><author>Lee, Seung-Cheol ; Lee, Seung-Ho ; Kang, Da-Hyun ; Kim, Min ; Sung, Jung-Suk ; Kadam, Avinash A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-c18e640b8114f4822568a00b5c748feec72759352d9fb9d4676d73e3be2aac673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alizarin</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bioorganic Chemistry</topic><topic>Cellulose</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Gene expression</topic><topic>Glass</topic><topic>Image enhancement</topic><topic>Iron oxides</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Natural Materials</topic><topic>Organic Chemistry</topic><topic>Original Research</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Staining</topic><topic>Stem cells</topic><topic>Sustainable Development</topic><topic>Synthesis</topic><topic>X ray photoelectron spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Seung-Cheol</creatorcontrib><creatorcontrib>Lee, Seung-Ho</creatorcontrib><creatorcontrib>Kang, Da-Hyun</creatorcontrib><creatorcontrib>Kim, Min</creatorcontrib><creatorcontrib>Sung, Jung-Suk</creatorcontrib><creatorcontrib>Kadam, Avinash A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Seung-Cheol</au><au>Lee, Seung-Ho</au><au>Kang, Da-Hyun</au><au>Kim, Min</au><au>Sung, Jung-Suk</au><au>Kadam, Avinash A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2023-03-01</date><risdate>2023</risdate><volume>30</volume><issue>4</issue><spage>2385</spage><epage>2398</epage><pages>2385-2398</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe
3
O
4
) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe
3
O
4
. The synthesized material αCS@Fe
3
O
4
was structurally and morphologically characterized by SEM, TEM, XRD, TGA, and XPS analyses. The characterizations confirmed a nanostructural modification of αCS using Fe
3
O
4
nanoparticles. Next, the fabricated material αCS@Fe
3
O
4
was assessed for biocompatibility. The obtained data confirmed the biocompatible nature of the αCS@Fe
3
O
4
. Then, the αCS@Fe
3
O
4
was applied for the osteoconductive differentiation of the hADSCs. The hADSCs osteoconduction was enhanced significantly (11.325 fold increase) in the presence of the αCS@Fe
3
O
4
compared to the control hADSCs. The Alizarin Red S (ARS) staining microscopic images corroborated the osteoconduction enhancement. Furthermore, the relative gene expression of the important osteogenic markers ALP, OCN, and RUNX2 was analyzed. The expression levels were significantly enhanced in the presence of the target material αCS@Fe
3
O
4
. Finally, the immunofluorescent staining of ALP, OCN, and RUNX2 corroborated the enhanced osteoconduction. Thus, in conclusion, αCS@Fe
3
O
4
is a low-cost, economical, biocompatible nano-biomaterial with significant osteoconduction enhancement potential that can be applied in bone defect treatments in the future.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10570-023-05045-7</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cellulose (London), 2023-03, Vol.30 (4), p.2385-2398 |
| issn | 0969-0239 1572-882X |
| language | eng |
| recordid | cdi_proquest_journals_2778478404 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Alizarin Biocompatibility Biomedical materials Bioorganic Chemistry Cellulose Ceramics Chemistry Chemistry and Materials Science Composites Gene expression Glass Image enhancement Iron oxides Nanomaterials Nanoparticles Natural Materials Organic Chemistry Original Research Physical Chemistry Polymer Sciences Staining Stem cells Sustainable Development Synthesis X ray photoelectron spectroscopy |
| title | Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement |
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