In vitro preparation of biosurfactant based herbal-nano topical ointment from Tridax procumbens infused oil using gelatin stabilized silver nanoparticle and its efficacy on fibroblastic cell lines
Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study, Tridax procumbens infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from Lactobacill...
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| Veröffentlicht in: | Applied nanoscience 2023, Vol.13 (1), p.719-734 |
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| creator | Kubendiran, Lavanya Theerthagiri, Sowmiya Al-Dhabi, Naif Abdullah Palaninaicker, Senthilkumar Subramanian, Shivakumar Muthugoundar Srinivasan, Venkatesan Karuppiah, Ponmurugan |
| description | Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study,
Tridax procumbens
infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from
Lactobacillus casei
(MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from
L. casei
confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely
Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa
and
Klebsiella pneumoniae
. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from
T. procumbens
oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound. |
| doi_str_mv | 10.1007/s13204-021-01896-3 |
| format | Article |
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Tridax procumbens
infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from
Lactobacillus casei
(MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from
L. casei
confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely
Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa
and
Klebsiella pneumoniae
. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from
T. procumbens
oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound.</description><identifier>ISSN: 2190-5509</identifier><identifier>EISSN: 2190-5517</identifier><identifier>DOI: 10.1007/s13204-021-01896-3</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Cell adhesion & migration ; Chemistry and Materials Science ; Cytotoxicity ; E coli ; Erythrocytes ; Gelatin ; Klebsiella ; Materials Science ; Membrane Biology ; Microorganisms ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Nanotechnology and Microengineering ; Ointments ; Original Article ; Pseudomonas aeruginosa ; Silver ; Surfactants</subject><ispartof>Applied nanoscience, 2023, Vol.13 (1), p.719-734</ispartof><rights>King Abdulaziz City for Science and Technology 2021</rights><rights>King Abdulaziz City for Science and Technology 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-fd80435e495c038030de84e0f1e0b492e99b14b0a9e656e70cd55832ddd0a1543</citedby><cites>FETCH-LOGICAL-c319t-fd80435e495c038030de84e0f1e0b492e99b14b0a9e656e70cd55832ddd0a1543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13204-021-01896-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13204-021-01896-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kubendiran, Lavanya</creatorcontrib><creatorcontrib>Theerthagiri, Sowmiya</creatorcontrib><creatorcontrib>Al-Dhabi, Naif Abdullah</creatorcontrib><creatorcontrib>Palaninaicker, Senthilkumar</creatorcontrib><creatorcontrib>Subramanian, Shivakumar Muthugoundar</creatorcontrib><creatorcontrib>Srinivasan, Venkatesan</creatorcontrib><creatorcontrib>Karuppiah, Ponmurugan</creatorcontrib><title>In vitro preparation of biosurfactant based herbal-nano topical ointment from Tridax procumbens infused oil using gelatin stabilized silver nanoparticle and its efficacy on fibroblastic cell lines</title><title>Applied nanoscience</title><addtitle>Appl Nanosci</addtitle><description>Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study,
Tridax procumbens
infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from
Lactobacillus casei
(MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from
L. casei
confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely
Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa
and
Klebsiella pneumoniae
. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from
T. procumbens
oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound.</description><subject>Cell adhesion & migration</subject><subject>Chemistry and Materials Science</subject><subject>Cytotoxicity</subject><subject>E coli</subject><subject>Erythrocytes</subject><subject>Gelatin</subject><subject>Klebsiella</subject><subject>Materials Science</subject><subject>Membrane Biology</subject><subject>Microorganisms</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nanotechnology and Microengineering</subject><subject>Ointments</subject><subject>Original Article</subject><subject>Pseudomonas aeruginosa</subject><subject>Silver</subject><subject>Surfactants</subject><issn>2190-5509</issn><issn>2190-5517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kU9rHSEUxYfQQkKaL9DVhaynvY7jzLgsoX8CgW7StahzfTX49EWd0PTz9YPVl1faXd0o3N8558rpurcM3zHE-X1hfMCxx4H1yBY59fysuxiYxF4INr_6-0Z53l2V8oDtiHGeuLjoft1GePI1JzhkOuisq08RkgPjU9my07bqWMHoQit8p2x06KOOCWo6eKsDJB_rnhrictrDffar_tG8kt32hmIBH9121CYfYCs-7mBHoaVEKFUbH_zPNiw-PFGGo3HboXobCHRcwdcC5FwLss_Q9nLe5GSCLg0BSyFA8JHKm-6106HQ1Z_7svv26eP9zZf-7uvn25sPd73lTNberQuOXNAohUW-IMeVlpHQMUIzyoGkNGw0qCVNYqIZ7SrEwod1XVEzMfLL7vrk2_73uFGp6iFtObZINcwzw0kuy9So4UTZnErJ5NQh-73Oz4qhOhamToWpVph6KUzxJuInUWlw3FH-Z_0f1W8wzJ3S</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Kubendiran, Lavanya</creator><creator>Theerthagiri, Sowmiya</creator><creator>Al-Dhabi, Naif Abdullah</creator><creator>Palaninaicker, Senthilkumar</creator><creator>Subramanian, Shivakumar Muthugoundar</creator><creator>Srinivasan, Venkatesan</creator><creator>Karuppiah, Ponmurugan</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2023</creationdate><title>In vitro preparation of biosurfactant based herbal-nano topical ointment from Tridax procumbens infused oil using gelatin stabilized silver nanoparticle and its efficacy on fibroblastic cell lines</title><author>Kubendiran, Lavanya ; Theerthagiri, Sowmiya ; Al-Dhabi, Naif Abdullah ; Palaninaicker, Senthilkumar ; Subramanian, Shivakumar Muthugoundar ; Srinivasan, Venkatesan ; Karuppiah, Ponmurugan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-fd80435e495c038030de84e0f1e0b492e99b14b0a9e656e70cd55832ddd0a1543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Cell adhesion & migration</topic><topic>Chemistry and Materials Science</topic><topic>Cytotoxicity</topic><topic>E coli</topic><topic>Erythrocytes</topic><topic>Gelatin</topic><topic>Klebsiella</topic><topic>Materials Science</topic><topic>Membrane Biology</topic><topic>Microorganisms</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nanotechnology and Microengineering</topic><topic>Ointments</topic><topic>Original Article</topic><topic>Pseudomonas aeruginosa</topic><topic>Silver</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kubendiran, Lavanya</creatorcontrib><creatorcontrib>Theerthagiri, Sowmiya</creatorcontrib><creatorcontrib>Al-Dhabi, Naif Abdullah</creatorcontrib><creatorcontrib>Palaninaicker, Senthilkumar</creatorcontrib><creatorcontrib>Subramanian, Shivakumar Muthugoundar</creatorcontrib><creatorcontrib>Srinivasan, Venkatesan</creatorcontrib><creatorcontrib>Karuppiah, Ponmurugan</creatorcontrib><collection>CrossRef</collection><jtitle>Applied nanoscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kubendiran, Lavanya</au><au>Theerthagiri, Sowmiya</au><au>Al-Dhabi, Naif Abdullah</au><au>Palaninaicker, Senthilkumar</au><au>Subramanian, Shivakumar Muthugoundar</au><au>Srinivasan, Venkatesan</au><au>Karuppiah, Ponmurugan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro preparation of biosurfactant based herbal-nano topical ointment from Tridax procumbens infused oil using gelatin stabilized silver nanoparticle and its efficacy on fibroblastic cell lines</atitle><jtitle>Applied nanoscience</jtitle><stitle>Appl Nanosci</stitle><date>2023</date><risdate>2023</risdate><volume>13</volume><issue>1</issue><spage>719</spage><epage>734</epage><pages>719-734</pages><issn>2190-5509</issn><eissn>2190-5517</eissn><abstract>Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study,
Tridax procumbens
infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from
Lactobacillus casei
(MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from
L. casei
confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely
Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa
and
Klebsiella pneumoniae
. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from
T. procumbens
oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s13204-021-01896-3</doi><tpages>16</tpages></addata></record> |
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| subjects | Cell adhesion & migration Chemistry and Materials Science Cytotoxicity E coli Erythrocytes Gelatin Klebsiella Materials Science Membrane Biology Microorganisms Nanochemistry Nanoparticles Nanotechnology Nanotechnology and Microengineering Ointments Original Article Pseudomonas aeruginosa Silver Surfactants |
| title | In vitro preparation of biosurfactant based herbal-nano topical ointment from Tridax procumbens infused oil using gelatin stabilized silver nanoparticle and its efficacy on fibroblastic cell lines |
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