Application of a quantitative PCR method for monitoring of geosmin-producing Anabaena spp. in a Japanese reservoir
Cyanobacteria blooms occur frequently in lakes and reservoirs worldwide and the blooms are often accompanied by changes in the taste and odor of the water caused by cyanobacterial metabolites, such as geosmin. The amount of geosmin in drinking water in Japan is limited legally and requires regular m...
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| Veröffentlicht in: | Landscape and ecological engineering 2023, Vol.19 (1), p.87-93 |
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| creator | Akamatsu, Yoshihisa Nakao, Ryohei Motozawa, Hiromu Furukawa, Koji Kurita, Yoshihisa |
| description | Cyanobacteria blooms occur frequently in lakes and reservoirs worldwide and the blooms are often accompanied by changes in the taste and odor of the water caused by cyanobacterial metabolites, such as geosmin. The amount of geosmin in drinking water in Japan is limited legally and requires regular monitoring. The approaches for detecting geosmin are generally based on gas chromatography–mass spectrometry (GC/MS), which requires experienced personnel or sophisticated instrumentation. Here, we applied a TaqMan probe method for detecting geosmin-producing cyanobacteria,
Anabaena
(
Dolichospermum
) spp. in a Japanese reservoir. Quantitative PCR amplification using the methods which targeted geosmin synthase gene, worked successfully and the DNA concentrations may correspond with both the number of
Anabaena
spp. cells and the geosmin concentration. The tests of suitable glass microfiber filters for monitoring
Anabaena
spp. revealed that the samples filtered through GF/D (particle retention size 2.7 µm) yielded significantly higher concentrations of
Anabaena
DNA compared with the samples filtered through the GF/F and GF/A. |
| doi_str_mv | 10.1007/s11355-022-00533-7 |
| format | Article |
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Anabaena
(
Dolichospermum
) spp. in a Japanese reservoir. Quantitative PCR amplification using the methods which targeted geosmin synthase gene, worked successfully and the DNA concentrations may correspond with both the number of
Anabaena
spp. cells and the geosmin concentration. The tests of suitable glass microfiber filters for monitoring
Anabaena
spp. revealed that the samples filtered through GF/D (particle retention size 2.7 µm) yielded significantly higher concentrations of
Anabaena
DNA compared with the samples filtered through the GF/F and GF/A.</description><identifier>ISSN: 1860-1871</identifier><identifier>EISSN: 1860-188X</identifier><identifier>DOI: 10.1007/s11355-022-00533-7</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Anabaena ; Biomedical and Life Sciences ; Civil Engineering ; Cyanobacteria ; Deoxyribonucleic acid ; DNA ; Drinking water ; Environmental Management ; Gas chromatography ; Geosmin ; Instrumentation ; Landscape Ecology ; Landscape/Regional and Urban Planning ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Microfibers ; Monitoring ; Monitoring methods ; Nature Conservation ; Plant Ecology ; Polymerase chain reaction ; Reservoirs ; Special Feature: Technical Note ; Water analysis</subject><ispartof>Landscape and ecological engineering, 2023, Vol.19 (1), p.87-93</ispartof><rights>The Author(s) under exclusive licence to International Consortium of Landscape and Ecological Engineering 2022</rights><rights>The Author(s) under exclusive licence to International Consortium of Landscape and Ecological Engineering 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-9a5943a010e41fad7bed99b06e80efda8c8bee7b688f2a1ceff831a09cad628d3</citedby><cites>FETCH-LOGICAL-c346t-9a5943a010e41fad7bed99b06e80efda8c8bee7b688f2a1ceff831a09cad628d3</cites><orcidid>0000-0001-6637-2075</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11355-022-00533-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11355-022-00533-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Akamatsu, Yoshihisa</creatorcontrib><creatorcontrib>Nakao, Ryohei</creatorcontrib><creatorcontrib>Motozawa, Hiromu</creatorcontrib><creatorcontrib>Furukawa, Koji</creatorcontrib><creatorcontrib>Kurita, Yoshihisa</creatorcontrib><title>Application of a quantitative PCR method for monitoring of geosmin-producing Anabaena spp. in a Japanese reservoir</title><title>Landscape and ecological engineering</title><addtitle>Landscape Ecol Eng</addtitle><description>Cyanobacteria blooms occur frequently in lakes and reservoirs worldwide and the blooms are often accompanied by changes in the taste and odor of the water caused by cyanobacterial metabolites, such as geosmin. The amount of geosmin in drinking water in Japan is limited legally and requires regular monitoring. The approaches for detecting geosmin are generally based on gas chromatography–mass spectrometry (GC/MS), which requires experienced personnel or sophisticated instrumentation. Here, we applied a TaqMan probe method for detecting geosmin-producing cyanobacteria,
Anabaena
(
Dolichospermum
) spp. in a Japanese reservoir. Quantitative PCR amplification using the methods which targeted geosmin synthase gene, worked successfully and the DNA concentrations may correspond with both the number of
Anabaena
spp. cells and the geosmin concentration. The tests of suitable glass microfiber filters for monitoring
Anabaena
spp. revealed that the samples filtered through GF/D (particle retention size 2.7 µm) yielded significantly higher concentrations of
Anabaena
DNA compared with the samples filtered through the GF/F and GF/A.</description><subject>Anabaena</subject><subject>Biomedical and Life Sciences</subject><subject>Civil Engineering</subject><subject>Cyanobacteria</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drinking water</subject><subject>Environmental Management</subject><subject>Gas chromatography</subject><subject>Geosmin</subject><subject>Instrumentation</subject><subject>Landscape Ecology</subject><subject>Landscape/Regional and Urban Planning</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Microfibers</subject><subject>Monitoring</subject><subject>Monitoring methods</subject><subject>Nature Conservation</subject><subject>Plant Ecology</subject><subject>Polymerase chain reaction</subject><subject>Reservoirs</subject><subject>Special Feature: Technical Note</subject><subject>Water analysis</subject><issn>1860-1871</issn><issn>1860-188X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKt_wFPA89Z8tLvZYyl-UlBEwVuY3Z3UlDbZJrsF_71ZK3rzMjMM7_vO8BByydmEM1ZcR87lbJYxITLGZlJmxREZcZWzjCv1fvw7F_yUnMW4TiIhBBuRMG_bja2hs95RbyjQXQ-us13a7JE-L17oFrsP31DjA916ZzsfrFsN2hX6uLUua4Nv-npYzh1UgA5obNsJtS7FPUILDiPSkErYexvOyYmBTcSLnz4mb7c3r4v7bPl097CYL7NaTvMuK2FWTiUwznDKDTRFhU1ZVixHxdA0oGpVIRZVrpQRwGs0RkkOrKyhyYVq5JhcHXLTf7seY6fXvg8undSiKBhTUvFpUomDqg4-xoBGt8FuIXxqzvTAVh_Y6sRWf7PVRTLJgym2AwwMf9H_uL4Adhx-kw</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Akamatsu, Yoshihisa</creator><creator>Nakao, Ryohei</creator><creator>Motozawa, Hiromu</creator><creator>Furukawa, Koji</creator><creator>Kurita, Yoshihisa</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-6637-2075</orcidid></search><sort><creationdate>2023</creationdate><title>Application of a quantitative PCR method for monitoring of geosmin-producing Anabaena spp. in a Japanese reservoir</title><author>Akamatsu, Yoshihisa ; Nakao, Ryohei ; Motozawa, Hiromu ; Furukawa, Koji ; Kurita, Yoshihisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-9a5943a010e41fad7bed99b06e80efda8c8bee7b688f2a1ceff831a09cad628d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anabaena</topic><topic>Biomedical and Life Sciences</topic><topic>Civil Engineering</topic><topic>Cyanobacteria</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drinking water</topic><topic>Environmental Management</topic><topic>Gas chromatography</topic><topic>Geosmin</topic><topic>Instrumentation</topic><topic>Landscape Ecology</topic><topic>Landscape/Regional and Urban Planning</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Microfibers</topic><topic>Monitoring</topic><topic>Monitoring methods</topic><topic>Nature Conservation</topic><topic>Plant Ecology</topic><topic>Polymerase chain reaction</topic><topic>Reservoirs</topic><topic>Special Feature: Technical Note</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akamatsu, Yoshihisa</creatorcontrib><creatorcontrib>Nakao, Ryohei</creatorcontrib><creatorcontrib>Motozawa, Hiromu</creatorcontrib><creatorcontrib>Furukawa, Koji</creatorcontrib><creatorcontrib>Kurita, Yoshihisa</creatorcontrib><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Landscape and ecological engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akamatsu, Yoshihisa</au><au>Nakao, Ryohei</au><au>Motozawa, Hiromu</au><au>Furukawa, Koji</au><au>Kurita, Yoshihisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of a quantitative PCR method for monitoring of geosmin-producing Anabaena spp. in a Japanese reservoir</atitle><jtitle>Landscape and ecological engineering</jtitle><stitle>Landscape Ecol Eng</stitle><date>2023</date><risdate>2023</risdate><volume>19</volume><issue>1</issue><spage>87</spage><epage>93</epage><pages>87-93</pages><issn>1860-1871</issn><eissn>1860-188X</eissn><abstract>Cyanobacteria blooms occur frequently in lakes and reservoirs worldwide and the blooms are often accompanied by changes in the taste and odor of the water caused by cyanobacterial metabolites, such as geosmin. The amount of geosmin in drinking water in Japan is limited legally and requires regular monitoring. The approaches for detecting geosmin are generally based on gas chromatography–mass spectrometry (GC/MS), which requires experienced personnel or sophisticated instrumentation. Here, we applied a TaqMan probe method for detecting geosmin-producing cyanobacteria,
Anabaena
(
Dolichospermum
) spp. in a Japanese reservoir. Quantitative PCR amplification using the methods which targeted geosmin synthase gene, worked successfully and the DNA concentrations may correspond with both the number of
Anabaena
spp. cells and the geosmin concentration. The tests of suitable glass microfiber filters for monitoring
Anabaena
spp. revealed that the samples filtered through GF/D (particle retention size 2.7 µm) yielded significantly higher concentrations of
Anabaena
DNA compared with the samples filtered through the GF/F and GF/A.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s11355-022-00533-7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6637-2075</orcidid></addata></record> |
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| subjects | Anabaena Biomedical and Life Sciences Civil Engineering Cyanobacteria Deoxyribonucleic acid DNA Drinking water Environmental Management Gas chromatography Geosmin Instrumentation Landscape Ecology Landscape/Regional and Urban Planning Life Sciences Mass spectrometry Mass spectroscopy Metabolites Microfibers Monitoring Monitoring methods Nature Conservation Plant Ecology Polymerase chain reaction Reservoirs Special Feature: Technical Note Water analysis |
| title | Application of a quantitative PCR method for monitoring of geosmin-producing Anabaena spp. in a Japanese reservoir |
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