Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4 in vitro and 1 HeLa cell in...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2023-01, Vol.59 (9), p.1181-1184 |
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| container_end_page | 1184 |
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| container_issue | 9 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Wang, Li-juan Lv, Meng-meng Hu, Jin-ping Liu, Meng Zhang, Chun-yang |
| description | We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4
in vitro
and 1 HeLa cell
in vivo
. Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. |
| doi_str_mv | 10.1039/d2cc06087f |
| format | Article |
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in vitro
and 1 HeLa cell
in vivo
. Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/d2cc06087f</identifier><identifier>PMID: 36628652</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Amplification ; Circuits ; HeLa Cells ; Humans ; Monitoring ; Telomerase ; Telomerase - metabolism</subject><ispartof>Chemical communications (Cambridge, England), 2023-01, Vol.59 (9), p.1181-1184</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-b66c31456a57817a30ae2c8d294d585b06691dd1cee1c254a7535b89b0fd8d2a3</citedby><cites>FETCH-LOGICAL-c337t-b66c31456a57817a30ae2c8d294d585b06691dd1cee1c254a7535b89b0fd8d2a3</cites><orcidid>0000-0002-8010-1981</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36628652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Li-juan</creatorcontrib><creatorcontrib>Lv, Meng-meng</creatorcontrib><creatorcontrib>Hu, Jin-ping</creatorcontrib><creatorcontrib>Liu, Meng</creatorcontrib><creatorcontrib>Zhang, Chun-yang</creatorcontrib><title>Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4
in vitro
and 1 HeLa cell
in vivo
. Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications.</description><subject>Amplification</subject><subject>Circuits</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Monitoring</subject><subject>Telomerase</subject><subject>Telomerase - metabolism</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0UtrFjEUBuAgir3oxr0ScCPC1Nwns5RPW4VCXSi4GzKZMzUllzHJYPsL_Nud6VcrNJvcnpwceBF6RckJJbz7MDJriSK6nZ6gQ8qVaKTQP59ua9k1LRfyAB2VckXWQaV-jg64UkwryQ7R3285Xbvg6g327tJUl2JTs4nFZjdvO2xdtourzZz-QIYRw_WcIsTqjMcmzN5Nzt69K3hKGRcXLz00IXmwiwccUnQ15fUUpwlX8ClANgWwi_jXEsz6AXhfXqBnk_EFXt7Px-jH6efvuy_N-cXZ193H88Zy3tZmUMpyKqQystW0NZwYYFaPrBOj1HIgSnV0HKkFoJZJYVrJ5aC7gUzjqgw_Ru_2deecfi9Qah9c2TowEdJSetYqIQSnlK_07SN6lZYc1-421clOMKFX9X6vbE6lZJj6Obtg8k1PSb_F039iu91dPKcrfnNfchkCjA_0Xx4reL0HudiH2__58lsiaJfD</recordid><startdate>20230126</startdate><enddate>20230126</enddate><creator>Wang, Li-juan</creator><creator>Lv, Meng-meng</creator><creator>Hu, Jin-ping</creator><creator>Liu, Meng</creator><creator>Zhang, Chun-yang</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8010-1981</orcidid></search><sort><creationdate>20230126</creationdate><title>Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells</title><author>Wang, Li-juan ; Lv, Meng-meng ; Hu, Jin-ping ; Liu, Meng ; Zhang, Chun-yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-b66c31456a57817a30ae2c8d294d585b06691dd1cee1c254a7535b89b0fd8d2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amplification</topic><topic>Circuits</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Monitoring</topic><topic>Telomerase</topic><topic>Telomerase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Li-juan</creatorcontrib><creatorcontrib>Lv, Meng-meng</creatorcontrib><creatorcontrib>Hu, Jin-ping</creatorcontrib><creatorcontrib>Liu, Meng</creatorcontrib><creatorcontrib>Zhang, Chun-yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Li-juan</au><au>Lv, Meng-meng</au><au>Hu, Jin-ping</au><au>Liu, Meng</au><au>Zhang, Chun-yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2023-01-26</date><risdate>2023</risdate><volume>59</volume><issue>9</issue><spage>1181</spage><epage>1184</epage><pages>1181-1184</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4
in vitro
and 1 HeLa cell
in vivo
. Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2023-01, Vol.59 (9), p.1181-1184 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_journals_2769594248 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Amplification Circuits HeLa Cells Humans Monitoring Telomerase Telomerase - metabolism |
| title | Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells |
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