Bothrops atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy
The use of anti-venom is one of the main control measures for snakebite envenoming when applied immediately after the snakebite. Systemic effects of the envenoming are usually reversed; however, neutralization of local effects is hardly achieved. The need for adjuvant therapies associated with serum...
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| Veröffentlicht in: | Lasers in medical science 2023-01, Vol.38 (1), p.53, Article 53 |
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| creator | Ferreira e Ferreira, Alex Augusto dos Reis, Valdison Pereira Santana, Hallison Mota Nery, Neriane Monteiro Evangelista, Jaína Rodrigues Serrath, Suzanne Nery da Silva Dutra, Ricardo Segundo Rego, Cristina Matiele Alves Tavares, Maria Naiara Macedo Silva, Milena Daniela Souza Soares, Andreimar Martins Rodrigues, Moreno Magalhães S. Zamuner, Stella Regina Zuliani, Juliana Pavan |
| description | The use of anti-venom is one of the main control measures for snakebite envenoming when applied immediately after the snakebite. Systemic effects of the envenoming are usually reversed; however, neutralization of local effects is hardly achieved. The need for adjuvant therapies associated with serum therapy can improve the treatment for local effects of envenoming, with greater effectiveness in preventing or delaying the progression of damage, reducing the clinical signs and symptoms of victims of snakebites. The purpose of the study was to evaluate the photobiomodulation therapy using LED and/or dexamethasone associated with conventional serum therapy for the treatment of local damage caused by
Bothrops atrox
envenomation in a murine model. For this, experimental envenoming was carried out in the gastrocnemius muscle of male Swiss mice weighing 18 to 22 g divided into 8 groups of animals, distributed in groups non-treat, treated with anti-bothropic serum, dexamethasone, and LED, or the associated treatments, by intramuscular inoculation of 50 µg of venom or sterile PBS (control). After 30 min, the proposed treatments were administered alone or in combination. After 3 h, blood and muscle samples were collected for myotoxicity, cytotoxicity, histological analysis, and IL-1β assays. The evaluation of the treatment alone showed that serum therapy is not effective for the treatment of local damage and photobiomodulation demonstrated to be an effective therapy to reduce leukocyte infiltration, hemorrhage, and myotoxicity in experimental envenoming; dexamethasone proved to be a good resource for the treatment of the inflammatory process reducing the leukocyte infiltration. The association of serum therapy, LED, and dexamethasone was the best treatment to reduce the local effects caused by
Bothrops atrox
venom. All in all, the association of photobiomodulation therapy using LED with conventional serum therapy and the anti-inflammatory drug is the best treatment for reducing the undesirable local effects caused by snakebite accidents involving
B. atrox
species. |
| doi_str_mv | 10.1007/s10103-023-03710-8 |
| format | Article |
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Bothrops atrox
envenomation in a murine model. For this, experimental envenoming was carried out in the gastrocnemius muscle of male Swiss mice weighing 18 to 22 g divided into 8 groups of animals, distributed in groups non-treat, treated with anti-bothropic serum, dexamethasone, and LED, or the associated treatments, by intramuscular inoculation of 50 µg of venom or sterile PBS (control). After 30 min, the proposed treatments were administered alone or in combination. After 3 h, blood and muscle samples were collected for myotoxicity, cytotoxicity, histological analysis, and IL-1β assays. The evaluation of the treatment alone showed that serum therapy is not effective for the treatment of local damage and photobiomodulation demonstrated to be an effective therapy to reduce leukocyte infiltration, hemorrhage, and myotoxicity in experimental envenoming; dexamethasone proved to be a good resource for the treatment of the inflammatory process reducing the leukocyte infiltration. The association of serum therapy, LED, and dexamethasone was the best treatment to reduce the local effects caused by
Bothrops atrox
venom. All in all, the association of photobiomodulation therapy using LED with conventional serum therapy and the anti-inflammatory drug is the best treatment for reducing the undesirable local effects caused by snakebite accidents involving
B. atrox
species.</description><identifier>ISSN: 1435-604X</identifier><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-023-03710-8</identifier><identifier>PMID: 36695923</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Animal bites ; Animal models ; Animals ; Anti-inflammatory agents ; Bothrops ; Bothrops atrox ; Crotalid Venoms ; Cytotoxicity ; Damage ; Dentistry ; Dexamethasone ; Dexamethasone - pharmacology ; Dexamethasone - therapeutic use ; Evaluation ; Gastrocnemius muscle ; Hemorrhage ; Infiltration ; Inflammation ; Inoculation ; Lasers ; Leukocytes ; Light emitting diodes ; Light therapy ; Male ; Medicine ; Medicine & Public Health ; Mice ; Muscle, Skeletal - pathology ; Muscles ; Myotoxicity - pathology ; Neutralization ; Optical Devices ; Optics ; Original Article ; Photonics ; Quantum Optics ; Signs and symptoms ; Snake Bites - drug therapy ; Steroids ; Therapy ; Toxicity ; Venom</subject><ispartof>Lasers in medical science, 2023-01, Vol.38 (1), p.53, Article 53</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-2185d9bd815ec09ea65fd0b8185960502b7e10bf45084d2d21d6717bd8da49733</citedby><cites>FETCH-LOGICAL-c375t-2185d9bd815ec09ea65fd0b8185960502b7e10bf45084d2d21d6717bd8da49733</cites><orcidid>0000-0001-9221-7749</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-023-03710-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-023-03710-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36695923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferreira e Ferreira, Alex Augusto</creatorcontrib><creatorcontrib>dos Reis, Valdison Pereira</creatorcontrib><creatorcontrib>Santana, Hallison Mota</creatorcontrib><creatorcontrib>Nery, Neriane Monteiro</creatorcontrib><creatorcontrib>Evangelista, Jaína Rodrigues</creatorcontrib><creatorcontrib>Serrath, Suzanne Nery</creatorcontrib><creatorcontrib>da Silva Dutra, Ricardo Segundo</creatorcontrib><creatorcontrib>Rego, Cristina Matiele Alves</creatorcontrib><creatorcontrib>Tavares, Maria Naiara Macedo</creatorcontrib><creatorcontrib>Silva, Milena Daniela Souza</creatorcontrib><creatorcontrib>Soares, Andreimar Martins</creatorcontrib><creatorcontrib>Rodrigues, Moreno Magalhães S.</creatorcontrib><creatorcontrib>Zamuner, Stella Regina</creatorcontrib><creatorcontrib>Zuliani, Juliana Pavan</creatorcontrib><title>Bothrops atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>The use of anti-venom is one of the main control measures for snakebite envenoming when applied immediately after the snakebite. Systemic effects of the envenoming are usually reversed; however, neutralization of local effects is hardly achieved. The need for adjuvant therapies associated with serum therapy can improve the treatment for local effects of envenoming, with greater effectiveness in preventing or delaying the progression of damage, reducing the clinical signs and symptoms of victims of snakebites. The purpose of the study was to evaluate the photobiomodulation therapy using LED and/or dexamethasone associated with conventional serum therapy for the treatment of local damage caused by
Bothrops atrox
envenomation in a murine model. For this, experimental envenoming was carried out in the gastrocnemius muscle of male Swiss mice weighing 18 to 22 g divided into 8 groups of animals, distributed in groups non-treat, treated with anti-bothropic serum, dexamethasone, and LED, or the associated treatments, by intramuscular inoculation of 50 µg of venom or sterile PBS (control). After 30 min, the proposed treatments were administered alone or in combination. After 3 h, blood and muscle samples were collected for myotoxicity, cytotoxicity, histological analysis, and IL-1β assays. The evaluation of the treatment alone showed that serum therapy is not effective for the treatment of local damage and photobiomodulation demonstrated to be an effective therapy to reduce leukocyte infiltration, hemorrhage, and myotoxicity in experimental envenoming; dexamethasone proved to be a good resource for the treatment of the inflammatory process reducing the leukocyte infiltration. The association of serum therapy, LED, and dexamethasone was the best treatment to reduce the local effects caused by
Bothrops atrox
venom. All in all, the association of photobiomodulation therapy using LED with conventional serum therapy and the anti-inflammatory drug is the best treatment for reducing the undesirable local effects caused by snakebite accidents involving
B. atrox
species.</description><subject>Animal bites</subject><subject>Animal models</subject><subject>Animals</subject><subject>Anti-inflammatory agents</subject><subject>Bothrops</subject><subject>Bothrops atrox</subject><subject>Crotalid Venoms</subject><subject>Cytotoxicity</subject><subject>Damage</subject><subject>Dentistry</subject><subject>Dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>Dexamethasone - therapeutic use</subject><subject>Evaluation</subject><subject>Gastrocnemius muscle</subject><subject>Hemorrhage</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Inoculation</subject><subject>Lasers</subject><subject>Leukocytes</subject><subject>Light emitting diodes</subject><subject>Light therapy</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscles</subject><subject>Myotoxicity - pathology</subject><subject>Neutralization</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Signs and symptoms</subject><subject>Snake Bites - drug 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atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy</title><author>Ferreira e Ferreira, Alex Augusto ; dos Reis, Valdison Pereira ; Santana, Hallison Mota ; Nery, Neriane Monteiro ; Evangelista, Jaína Rodrigues ; Serrath, Suzanne Nery ; da Silva Dutra, Ricardo Segundo ; Rego, Cristina Matiele Alves ; Tavares, Maria Naiara Macedo ; Silva, Milena Daniela Souza ; Soares, Andreimar Martins ; Rodrigues, Moreno Magalhães S. ; Zamuner, Stella Regina ; Zuliani, Juliana Pavan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-2185d9bd815ec09ea65fd0b8185960502b7e10bf45084d2d21d6717bd8da49733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal bites</topic><topic>Animal models</topic><topic>Animals</topic><topic>Anti-inflammatory agents</topic><topic>Bothrops</topic><topic>Bothrops 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Sci</addtitle><date>2023-01-25</date><risdate>2023</risdate><volume>38</volume><issue>1</issue><spage>53</spage><pages>53-</pages><artnum>53</artnum><issn>1435-604X</issn><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>The use of anti-venom is one of the main control measures for snakebite envenoming when applied immediately after the snakebite. Systemic effects of the envenoming are usually reversed; however, neutralization of local effects is hardly achieved. The need for adjuvant therapies associated with serum therapy can improve the treatment for local effects of envenoming, with greater effectiveness in preventing or delaying the progression of damage, reducing the clinical signs and symptoms of victims of snakebites. The purpose of the study was to evaluate the photobiomodulation therapy using LED and/or dexamethasone associated with conventional serum therapy for the treatment of local damage caused by
Bothrops atrox
envenomation in a murine model. For this, experimental envenoming was carried out in the gastrocnemius muscle of male Swiss mice weighing 18 to 22 g divided into 8 groups of animals, distributed in groups non-treat, treated with anti-bothropic serum, dexamethasone, and LED, or the associated treatments, by intramuscular inoculation of 50 µg of venom or sterile PBS (control). After 30 min, the proposed treatments were administered alone or in combination. After 3 h, blood and muscle samples were collected for myotoxicity, cytotoxicity, histological analysis, and IL-1β assays. The evaluation of the treatment alone showed that serum therapy is not effective for the treatment of local damage and photobiomodulation demonstrated to be an effective therapy to reduce leukocyte infiltration, hemorrhage, and myotoxicity in experimental envenoming; dexamethasone proved to be a good resource for the treatment of the inflammatory process reducing the leukocyte infiltration. The association of serum therapy, LED, and dexamethasone was the best treatment to reduce the local effects caused by
Bothrops atrox
venom. All in all, the association of photobiomodulation therapy using LED with conventional serum therapy and the anti-inflammatory drug is the best treatment for reducing the undesirable local effects caused by snakebite accidents involving
B. atrox
species.</abstract><cop>London</cop><pub>Springer London</pub><pmid>36695923</pmid><doi>10.1007/s10103-023-03710-8</doi><orcidid>https://orcid.org/0000-0001-9221-7749</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1435-604X |
| ispartof | Lasers in medical science, 2023-01, Vol.38 (1), p.53, Article 53 |
| issn | 1435-604X 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_journals_2768982547 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal bites Animal models Animals Anti-inflammatory agents Bothrops Bothrops atrox Crotalid Venoms Cytotoxicity Damage Dentistry Dexamethasone Dexamethasone - pharmacology Dexamethasone - therapeutic use Evaluation Gastrocnemius muscle Hemorrhage Infiltration Inflammation Inoculation Lasers Leukocytes Light emitting diodes Light therapy Male Medicine Medicine & Public Health Mice Muscle, Skeletal - pathology Muscles Myotoxicity - pathology Neutralization Optical Devices Optics Original Article Photonics Quantum Optics Signs and symptoms Snake Bites - drug therapy Steroids Therapy Toxicity Venom |
| title | Bothrops atrox mice experimental envenoming treatment using light-emitting diode (led) as an adjunct therapy to conventional serum therapy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T03%3A21%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bothrops%20atrox%20mice%20experimental%20envenoming%20treatment%20using%20light-emitting%20diode%20(led)%20as%20an%20adjunct%20therapy%20to%20conventional%20serum%20therapy&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Ferreira%20e%20Ferreira,%20Alex%20Augusto&rft.date=2023-01-25&rft.volume=38&rft.issue=1&rft.spage=53&rft.pages=53-&rft.artnum=53&rft.issn=1435-604X&rft.eissn=1435-604X&rft_id=info:doi/10.1007/s10103-023-03710-8&rft_dat=%3Cproquest_cross%3E2768982547%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2768982547&rft_id=info:pmid/36695923&rfr_iscdi=true |